• Journal of Animal Science and Technology
• /
• v.49 no.1
• /
• pp.79-88
• /
• 2007

This study was conducted to determine the minimal yield of spent mushroom substrates (SMS) and physicochemical characteristics of Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes according to mushroom species and cultivation types. The annual yield of SMS in 2004 was minimally 1,670,182M/T and 10.7-fold of the mushroom yield. The yield of SMS for Pleurotus ryngii, Pleurotus osteratus and Ammulina velutipes was 972,141M/T and was 58.2% of the total yield of SMS. Data from the chemical analysis of totally 109 SMS samples revealed that bed type cultivation showed low NDF (65.2%), high NFC (12.7%) and high ash (11.5%) contents (P<0.05), resulting in better nutritional characteristics compared with bottle or vinyl bag cultivation. In general, it was more desirable to classify SMS by cultivation types rather than by mushroom species for the effective use of SMS as an animal feed. Among cultivation types, SMS from bed type cultivation needed to be preferably used as feed.

• Journal of Mushroom
• /
• v.5 no.2
• /
• pp.76-80
• /
• 2007

After bottle culture of Pleurotus eryngii, media were taken out the bottle and normally utilized compost. However, nutritional elements were remained in the waste media. This study was carried out to investigate the reusable possibility and the optimal additive rate of waste media in an artificial cultivation of Pleurotus eryngii. The pH had tendency to decrease as the waste media was added from 6.0 to 4.8. Based on the additive rate of 10, 20, 30, 40, and 50%, each treatment waste media was added to new media for culturing Pleurotus eryngii. Among various treatments, the mycelial growth and primordia formation of Pleurotus eryngii were more favorable in the addition of 10-30% waste median than in the addition of 40 and 50%. The yield of its fruiting body was increased slightly in the treatment of 10-30% waste media.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.18 no.4
• /
• pp.45-53
• /
• 2010

Feeding rate and growth rate of earthworm population on the variously pretreated spent materials of the agaric-mushroom cultivation were investigated. When the spent mushroom substrates with different aging periods were supplied to earthworm, feeding rate and growth rate of earthworm population on spent mushroom substrates aged less than 10 days were higher than that on spent mushroom substrates aged more than 20 days. Feeding rate and growth rate were not increased when the spent mushroom substrate mixed with vermicasts or nitrogenous fertilizer was supplied. Feeding rate and growth rate on the ground mushroom substrate were higher than that on the non-ground mushroom substrate. Especially when the ground mushroom substrate was mixed with rice bran and supplied to earthworms, growth rate was much higher than that on the non-ground spent mushroom substrate; it increased 1.85 times.

• Journal of Animal Science and Technology
• /
• v.48 no.2
• /
• pp.237-246
• /
• 2006

This study was conducted to evaluate the feed-nutritional value of spent mushroom substrates(SMS) as a roughage source for ruminants through in vitro and in situ experiments. The SMS was classified into a roughage source with high fiber(NDF 64～78%) and low protein(CP 7～11%). The chemical composition of SMS was affected mainly by the primary culture ingredient rather than mushroom species. Compared with sawdust-SMS, cotton waste-SMS contained less(P<0.05) NDF and more(P<0.05) nonfibrous carbohydrate and ash(P<0.05). In vitro DM and NDF disappearances were high in the order of corn cob-, cotton waste-, and sawdust-SMS, in situ DM, NDF and ADF disappearances at 24hr incubation also showed the same pattern with in vitro trials. Compared with sawdust-SMS, cotton waste-SMS had higher digestible fractions and lower non-digestible fractions of NDF and ADF(P<0.05), resulting in higher in situ DM and NDF disappearances(P<0.05) and higher ruminal degradability(P<0.05). Therefore, the preferential use of cotton waste-SMS to sawdust-SMS is recommended as a roughage source for ruminants.

• Journal of Mushroom
• /
• v.7 no.1
• /
• pp.22-26
• /
• 2009

These experiments were conducted to find the optimum mixing rate of used media for saving the production cost of Flammulina velutipes. The materials for media formation was used needle-leaf tree sawdust, media that finished 1th cultivation of F. velutipes, corncob meal, and rice bran, wheat bran as nutrition source. We inoculated the F. velutipes in 14 kinds of different media types and checked the spawn growth speed, fruit body quality and quantity. Two nutrition agents, which is rice bran and wheat bran, did not affected the incubation period, but the effective stem number, quality and quantity of fruit body was better at rice bran than wheat bran. The quality of fruit body produced at mixed 20% of used media (needle-leaf tree sawdust 60% + used media 20% + rice bran 20%) was similar to control plot (needle-leaf tree sawdust 80% + rice bran 20%), but the yield was improved 10% than control plot 130g. According as the used-media mixing amount increases, quality and quantity of fruit body became low remarkably. Therefore, the optimum mixing amount of used-media was 20% and it increased 10% of fruitbody yield.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.7 no.1
• /
• pp.118-130
• /
• 2005

This study was conducted to evaluate the spent composts of selenium-enriched mushrooms as a feed selenium Source. Total selenium (Se) contents and Se profiles in the spent mushroom composts (SMC) were determined. In addtion, we also investigated the metabolism in relation to Se accumulation in the mushroom. Mushrooms used in this study were Flammulina velutipes and Se enriched mushrooms were grown for 60 days by adding 2 mg of inorganic Se (Na₂SeO₃) per kg of mushroom composts (MC) on as-fed basis and it was compared with mushrooms not to add Se to the MC. Total Se contents for Se-treated mushrooms were significantly increased (P<0.0001) by 20-fold (4.51 ㎍/g of dry) compared to Se-untreated (0.23 ㎍/g of dry). On the contrary, organic Se proportion was significantly lower (P<0.0001) in the Se-treated mushroom (72.3%) than Se-untreated (100%, not analytically detected of inorganic Se). Se distribution upon a length in the Se-treated mushrooms was the highest in the bottom part (6.86 ㎍/g of dry) near to MC, and top and middle parts were significantly lower (3.71 and 3.01 ㎍/g of dry, respectively) than the bottom (P<0.001). In the SMC from Se-treated mushrooms, a high concentration of Se (5.04 ㎍/g of dry) was still remained, but that from Se-untreated mushrooms was significantly low (P<0.0001) as 0.08 ㎍/g of dry. Se-treated SMC showed a high rate of organic Se (65.67%), suggesting that most of inorganic Se in the SMC was converted to organic Se by mushroom mycelia, and Se-untreated SMC showed 100% of organic Se, not being detected of inorganic Se. Prior to mycelia inoculation in the mushroom culture, the sterilization of MC brought approximately 18% of Se loss in the MC. This result is in accordance with facts generally known that Se is weak in the high temperature and it is consequently volatilized under that condition. Apparent and net accumulation rates (%) for Se into mushrooms were 14.81 and 10.14%, respectively and their difference (4.67%) is considered that it is due to the volatilization into the air via metabolic process of mushroom itself. From the result of this study, inorganic Se addition to MC for mushroom improved the Se content in the mushroom and SMC from Se-enriched mushrooms contained a high concentration of Se. Mycelium and fruiting body from mushrooms converted inorganic Se in MC to organic Se, indicating a high proportion of organic Se in the mushroom and SMC. Therefore, Se in Se-enriched mushroom and SMC was recognized as Se sources of food for human as well as feed for livestock.

• Korean journal of applied entomology
• /
• v.53 no.4
• /
• pp.367-373
• /
• 2014

In order to investigate the impact of recycling agricultural wastes as feed for mealworm (Tenebrio molitor), we evaluated the replacing effect by the different level of tangerine shell, Chinese cabbage, king oyster mushroom (Pleurotus eryngii) and Enoki mushroom (Flammulina velutipes) substrates on wheat bran feed. Larval survival rate, larval weight, developmental period of larva, pupation rate and pupal weight were evaluated. In tangerine shell and Chinese cabbage replacement group, no replacing effects found. In all groups replacing by spent King oyster mushroom (Pleurotus eryngii) substrate, survival rate of larva was similar to that of control group but larvae weighed less than control group significantly. Developmental period of larva increases in the group of King oyster mushroom substrate replacement. Larval and pupal weight in Enoki mushroom (Flammulina velutipes) substrate by the level of 40 and 50%, replacement effect showed best results in successive breeding considering pupation rate. It is concluded that replacing 40 and 50% of Enoki mushroom (Flammulina velutipes) substrate is appropriate to substituted diet of Tenebrio molitor larvae.

• Feed Journal
• /
• v.4 no.5 s.33
• /
• pp.94-98
• /
• 2006

농진청 한국농업전문학교 김완영 교수와 축산연구소 박범영 박사 연구팀은 농림부(농림기술특정과제)의 지원과 전남 영암소재 믿음영농조합법인(대표:류호진)의 참여로 버섯 폐배지를 이용하여 셀레늄이 강화된 새로운 기능성 한우고기인 "셀렌미트(Selen-Meat)"를 개발하는데 성공하였다.

• Journal of Mushroom
• /
• v.12 no.1
• /
• pp.17-23
• /
• 2014

pH of oriental medicine sludge was 5.3, which was similar to 5.2 of the main ingredient, corncob. Its sugar content, however, was 4.8 mg/g, which was 2.5 times higher than concorb's 1.9 mg/g. According to the addition content analysis of oriental medicine sludge by using blood agar plate, the experimental group showed much more robust growth than the control group. 10% of oriental medicine sludge was added to corncob and pine tree sawdust for test-tube culture. Then they were cultivated at $25^{\circ}C$ for 6 days after inoculating Flammulina velutipes liquid spawn. The control group and experimental group showed 2.2~3.4 and 5.8~6.4 cm hypae growths respectively. At the field test for 10% herbal medicine refuse, mushroom yield dropped by 5% compared to the control group. However, it had distinctively lower number of deformity and the 2nd grade products. An economic analysis was conducted based on the cultivation facility that produces 160,000 mushrooms per day. The analysis demonstrated that the facility can save 50,000,000 won in the starting year and 130,000,000 won in the following years from the unit cost of production excluding labour and operation cost.

• Journal of Animal Science and Technology
• /
• v.51 no.3
• /
• pp.241-248
• /
• 2009

Metabolic trial with 3 fistulated sheep was conducted in a 3 $\times$ 3 Latin square design and feeding trial with 24 Hanwoo steers in 12 month of age for 20 months was conducted to investigate the replacing effect of rice straw with fermented spent mushroom (Flammuliua velutipes) compost (FSMC) on fermentation characteristics, ruminal effective degradabilty and whole tract digestibility of nutrients in sheep, and to examine the growth performance of Hanwoo steers. Experimental diets for the metabolic trial with sheep were commercial concentrates and rice straw in the ratio of 70 : 30 (CON, DM basis). Same concentrate with 30% FSMC and 70% rice straw (FSMC-30) and 60% FSMC and 40% rice straw(FSMC-60). Diets for Hanwoo steers in three treatments were same as for metabolic trial in replacing ratio of rice straw with FSMC. pH of rumen fluid in sheep was not affected by FSMC. Ammonia-N content in the rumen fluid was highest in the sheep fed FSMC-60 at 3h (P<0.045). The CON diet increased (P<0.001) acetate proportion at 1h and 3h post feeding compared to FSMC-60 diet while propionate proportion was highest in the sheep fed FSMC-60 diet for all the sampling times (P<0.027~P<0.002). Increased proportion of butyrate was observed at 30 min prior to feeding (P<0.031), and 1h (P<0.011) and 6h(P<0.039) post feeding from sheep fed FSMC-30 diet compared to those from sheep fed other diets. Effective degradability in the rumen was not influenced by experimental diets. Whole tract digestibility of crude protein (P<0.031) and neutral detergent fiber (P<0.006) tended to be increased in the sheep fed CON diet while corresponding values were lowest in the sheep fed FSMC-60 diet. Total body weight gain of Hanwoo steers for 8 months was not different among diets, thus daily body gain was not influenced by the experimental diets.