• Title/Summary/Keyword: 페리

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Expression of Human Lactoferricin in HC11 Cells (HC11 세포에서 인체 락토페리신의 발현)

  • Nam, Myoung-Soo
    • Korean Journal of Agricultural Science
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    • v.28 no.2
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    • pp.92-98
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    • 2001
  • Lactofenicin is an antibacterial peptide fragment (about 5 kD) derived from lactoferrin (80 kD) that displays the various biological functions. The production of a human lactoferricin (Lactoferricin H) in mouse HC11 mammary epithelial cells was achieved by placing its cDNA under the control of the bovine ${\beta}$-casein gene. To express lactoferricin H in this cell culture system, constructed a hybride-splice signal consisting of bovine ${\beta}$-casein intron I and rabbit ${\beta}$-globin intron II, and a DNA fragment spanning intron 8 of the bovine ${\beta}$-casein gene. Expression of lactofenicin H from this expression vector was identified by RT-PCR, northern and dot blot analysis. RT-PCR using total RNA of HC11 cells transfected with pBL1-cin expression vector yielded a product identified as having a size of the 150bp. Northern blot analysis was identified about 2.3 kb. In dot blot analysis, recombinant lactofenicin H was recognized with anti-human lactofrrnin polyclonal antibody.

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Pyran and Pyridine as Building Blocks in Heterocyclic Synthesis (이중고리 합성에 블록제로서 이용된 피란과 피리딘)

  • El-Hashash, Maher.A.;El-Sawy, Abdallah.A.;Eissa, Abdelmonem.M.F.
    • Journal of the Korean Chemical Society
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    • v.53 no.3
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    • pp.308-324
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    • 2009
  • The present work is devoted to study the interaction of $\beta$-aroylacrylic acid derivative (3) with malononitrile in (DMF) in the presence of piperidine and/or ammonium acetate, then using the formed compounds as starting materials for synthesizing fused and isolated heterocyclic systems. It has been established that the $\beta$-aroylacrylic acid (3) reacts with malononitrile in (DMF) in the presence of piperidine as a catalyst with the formation of 4H-pyran derivative (4). By changing the catalyst into ammonium acetate, pyridine derivative (5) has been obtained. Also the N-maleamic acid derivatives (19) and (27) have been synthesized via the interaction of (4) and (5) with maleic anhydride. The purpose of this step is to study the behavior of the formed maleamic acid derivatives – as analogies of $\beta$-aroylacrylic acids – towards different active methylene compounds under Michael addition reaction.

A Study on the Direction of Christian education in the Age of hyper connectivity Society (초연결성 사회에서의 기독교교육 방향 모색)

  • Chung, Ha Eun
    • Journal of Christian Education in Korea
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    • v.71
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    • pp.371-399
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    • 2022
  • The era we are living in is an era of hyperconnectivity where boundaries and limitations of each field and domain disappear and organically converge and share with each other. Christians living in the age of hyperconnectivity are losing their direction of life due to various divisions and severances, such as holiness and secularity, church and world, soul and body, faith and life, and humans and nature. However, in a hyperconnected society, it is necessary to break free from division and conflict caused by disconnection, and realize the kingdom of God through connection and solidarity between humans, nature, and the world. In order to explore the direction of Christian education for this purpose, this study examined the characteristics of the era of hyperconnectivity and the principle of solidarity, which is the core of hyperconnectivity. The theological meaning of solidarity was examined in terms of humans, nature, and the world, and based on this understanding, the direction of Christian education in the era of hyperconnectivity was sought. It can be summarized as having a religious understanding of human beings of Homoconnectus with a pericoretic mode of existence. Third, education on the kingdom of justice and peace where we can live together in a solidarity relationship can be summarized.

Textural Implications of Fine-Grained Peridotite Xenoliths in Basaltic Rocks from Jeju Island (제주도 현무암에 포획된 세립질 맨틀 페리도타이트 포획암의 조직적 특성)

  • Yang, Kyoung-Hee;Nam, Bok-Hyun;Kim, Jin-Seop;Szabo, Csaba
    • Journal of the Mineralogical Society of Korea
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    • v.22 no.1
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    • pp.1-11
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    • 2009
  • Fine-grained peridotite xenoliths are rarely trapped in the basaltic rocks from the southeastern part of Jeju Island. Based on textural characteristics of the constituent phases showing uniform-sized, fine-grained tabular to mosaic grains with rare porphyroclastic relics, the studied samples can be defined as fine-grained, foliated porphyroclastic peridotites (FPP). Almost no significant difference among the FPPs in textures and major element compositions implies that the FPPs were derived from a structural domain, experiencing similar deformation events and deformation patterns. Moreover, the bimodal distribution with kink-banded porphyroclasts ($2{\sim}3mm$) and stain-free neoblasts ($200{\sim}300{\mu}m$), straight to gently curved grain boundaries with triple junctions, interstitial melt pockets, and microstructures for migrating grain boundary suggest that the studied samples went through dynamic recrystallization (${\pm}$ static recrystallization) in the presence of melt/fluid movement along foliation planes. No notable difference between the FPP and common protogranular xenoliths in major element compositions and geochemical evolution also implies that the FPP and protogranular xenoliths were from a similar horizon. Thus, the textural and geochemical characteristics of the FPPs reflects deformation events occurred at a localized and narrow zone within the lithospheric mantle beneath the Jeju Island. Although further detailed studies are necessary to define deformation events, the most possible process which could trigger deformation in the FPP in the rigid upper mantle was the ascending basaltic magma forming high-stress deformation zones. The suggested high-stress deformation zones in the lithosphere beneath the Jeju Island may be produced by paleo-faulting events related to the ascent of basalt magma before Jeju Island was formed.

Seismic Performance Evaluation of Railway Bridges Using Spherical Elastomeric Bearing (스페리컬 탄성받침을 이용한 철도교량의 내진성능평가)

  • Oh, Ju;Lee, Jae-Uk;Kim, Hu-Seung;Kim, Jae-Wook;Park, Seong-Kyu
    • Proceedings of the KSR Conference
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    • 2011.05a
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    • pp.1236-1241
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    • 2011
  • Railway bridge is the most problematic thing in the railway is due to noise and vibration. To solve these problems, there have been studies. However, a fundamental alternative to the noise and vibration. It has not so far not shown, and to minimize the problem is focused. As a result, developed a lot of noise reduction measures, but the vibration is not much for reduction. In this study, to support the superstructure of the bridge vibration possible for spherical elastomeric bearing is a technical review. And it applies to the railway bridge, the numerical analysis was carried out.

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Reduce in Cosmetic Packaging (화장품 용기감량화 사례 및 지속가능 제품 개발)

  • (사)한국포장협회
    • The monthly packaging world
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    • s.253
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    • pp.50-57
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    • 2014
  • LG생활건강은 '고객의 아름다움과 꿈을 실현하는 최고의 생활문화기업'이 되고자 Healthy, beautiful, Refreshing의 3개 영역에서 소비자에게 가치를 제공하기 위한 사업을 펼치고 있는 매출규모 4조원의 FMCG(Fast Moving Consumer Goods) 회사이다. LG생활건강의 지속가능경영보고서에 따르면 프레스티지 화장품 부문은 기존 브랜드가 지속적으로 성장하는 가운데 스타제품 육성 등 신규 브랜드들의 성과를 자랑하고 있으며 매스티지 화장품 부문은 친환경제품에 대한 소비자들의 높아진 관심 속에 에코브랜드 '비욘드'가 급성장 한 특징을 들 수 있다. 생활용품 사업은 엘라스틴, 테크, 페리오, 샤프란 등 메가브랜드들이 이끄는 주요 카테고리들이 꾸준히 성장해 2위 업체와의 격차를 더욱 벌리며 지속적으로 시장지배력을 높여가고 있다. 이 외에도 음료 사업은 소비자 선택의 폭을 넓히는 다양한 제품보강 및 유통 커버리지 확대 등으로 지속적인 패키지 이노베이션과 소비자 선택의 폭을 넓히며 전 브랜드가 고르게 성장했다. 본 고에서는 LG생활건강의 화장품용기 감량화 사례 및 지속가능 제품 개발 사례에 대해 살펴보도록 한다.

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Characteristics of Micromiling, Farina Milling, and Cooling Quality of Farina Spaghetti from Kansas Hard Red Winter Wheat (켄사스 경질 붉은밀의 소규모 시험 제분 및 페리나 제분 특성과 이들로부터 제조된 스파게티의 조리특성)

  • 김혁일;김창순
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.4
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    • pp.686-697
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    • 1994
  • The hardness of hard red winter (HRW) wheat had an effect on the yield of farina , as it positively correlated with the coarse fraction (over 34W) and negatively correlated with the flour fraction from farina milling. But it did not show any significances on flour milling from the micromilling process. The flour yields was better correlated with the break flour fraction (r=0.730 than with reduction flour (r=0.27). The farina yield was controlled by a fraction over 34W (coarse granule0. Protein was the most important single factor for the quality of cooked farina spaghetti, but not for flour milling or farina milling. On the other hand, hardness was important in the production of farina milling , whereas it did not govern spaghetti cooking quality, Environmental factors affected the quality of cooked spaghetti as much as varieties of HRW wheats. The quality of cooked farina spaghetti generally correlated with the protein in the location composites of wheats. Higher protein content wheats showed better spaghetti cooking quality.

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융합 페리틴의 요소 농도에 따른 재접힘 특성에 관한 연구

  • Kim, Hyeong-Won;Sin, Mi-Yeong;An, Eun-Gyeong;Kim, In-Ho
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.480-483
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    • 2003
  • Fusion ferritin$(F_H+F_L)$, an iron-binding protein, was purified from recombinant E. coli by two-step sonications with urea. Unfolded ferritin was refolded by gel filtration chromatography with various concentration of urea. 50 mM Tris-HCl(pH 8.0) buffers with 1 M to 4 M urea were used in GFC. Objective was to characterize the structure change with urea concentration. Molecular weight was determined using GF-HPLC and RP-HPLC was used to quantify the unfolded and refolded proteins.

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Optimum Grinding Condition for Electroplated Diamond Wheel in Form Grinding of Ferrite (페리이트의 총형 연삭에서 전착 다이아몬드 연삭숫돌의 최적 연삭조건)

  • 김성청;이재우;김관우;한상욱;황선희
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1996.04a
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    • pp.29-33
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    • 1996
  • This paper aims to clatify the potimum grinding condition for the electroplated diamond wheel in form grinding of Sr-ferrite. The main conclusions obtained were as follows. (1) The flexural strength and surface roughness of ferrite became the highest at the peripheral wheel speed of 1700m/min. (2) In the case of depth of cut larger than 0.4mm, crack layers is induced in the ground surface, the fracture type of chips exhibits slight ductile mode in the depth of cut smaller than 0.2mm. (3) When the depth of cut exceed 0.6mm, the tool life becomes extermely short due to large chipping and brackage. However, at the depth of cut .geq. 0.05mm, the diamond grain shows abrasive wear. (4) The flexural strength and surface roughness increases in proportion to the feed rate.

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Identification of Ferritin Using Immunodiffusion Methods (면역확산법을 이용한 페리친의 확인)

  • Ha, Kwang-Won;Cho, Jung-Hee;Kim, Do-Hoon;Kim, Young-Lim;Kim, Hong-Jin;Shim, Young-Hun
    • Korean Journal of Pharmacognosy
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    • v.33 no.3 s.130
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    • pp.257-261
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    • 2002
  • Each ferritin molecule consists of light subunit 19,000 dalton and heavy subunit 22,000 dalton. Twenty-four protein subunit about $440,000{\sim}500,000$ dalton apoferritin which contained $20{\sim}30%$ Fe as ferric hydroxyphosphate polymer form. Horse spleen-derived ferritin consists of 90% light subunit. These genetic characteristics of ferritin preparations were able to determine by cellulose acetate electrophoresis, but these ferritin preparations contained other components to be disturbed during refining, extraction and making finish products and have difficulties in deciding to be just. So, this study was performed to establish the scientific method for determine the quality of ferritin preparations with immunodiffusion methods which has high specificity between heterogeneous proteins.