• Journal of the Korean Society of Food Science and Nutrition
• /
• v.33 no.5
• /
• pp.769-777
• /
• 2004

A Helicobacter pylori urease inhibitor from Rubus coreanus Miquel has been isolated and partially characterized for aiming to Prevent H. pylori growth and decrease harmful accumulation of ammonia in human gastric mucosa. We screened urease inhibitory activities in 519 extracts library prepared by solvent extraction from 173 kinds of edible plants, medicinal herbs, herbs and seaweeds using a colorimetric urease assay system. As results of primary and secondary screening, 70% acetone extract of Rubus coreanus Miquel was selected as potent candidate, showing about 24% inhibitory activity. The acetone extract was sequentially partitioned into RCE/RCWI and RCB/RCW2 layers with ethyl acetate and butanol. The major active component in RCW2, water layer from butanol fractionation was revealed to be peptidic or proteinous substance by inhibitory activity determination after pronase digestion and periodate oxidation. RCW2-IIIc a was isolated by sequential column chromatography on DEAE-Toyopearl 650C, Butrl-Toyopearl 650M and Sephadex LH-20. The isolated urease inhibitor RCW2-IIIc $\alpha$, was highly pure proteinous substance with molecular weight of 13kDa by high-performance gel permeation liquid chromatography. RCW2-IIIc$\alpha$ has about 5 times higher inhibitory activity than 70％ acetone extract, showing high stability against heat treatment and peptic digestion.

• Journal of agriculture & life science
• /
• v.43 no.6
• /
• pp.1-6
• /
• 2009

The purpose of this study was to analyze annual tree ring growth characteristics for major tree species distributed in Chungbuk province. A total of 800 sample trees from 56 permanent sampling plots measured by the 5th Korean National Forest Inventory Program in 2007 was used for the calculation of annual growth rates. According to the results of this study, the species of Robinia pseudoacacia(2.30mm/yr) showed the best annual tree ring growth rates and the others are Quercus serrata(2.27mm/yr)>Prunus sargentii(1.98mm/yr)> and Larix leptolepis(1.98mm/yr) in order. Most of the major tree species in Chungbuk province, as tree age and stand density increased, annual tree ring growth rates tended to decreased. This information could be very useful for forest managers to understand annual tree ring growth characteristics in Chungbuk province.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2019.05a
• /
• pp.214-214
• /
• 2019

가뭄이 '심함' 단계 이상 도달 시에는 매주 수문분석을 수행하여 가뭄전망을 수행하여야 한다. 이를 위해서는 기상청의 강수량과 기온 등의 기상예측 자료가 필요하다. 현재 기상청에서는 3개월 기상전망으로 월단위 강수량과 평균기온을 매월 제공하고 있다. 1개월 전망에서 4주의 강수량합과 평균기온을 제공하고 있다. 하지만, 향후 4주간을 전망하는 1개월 전망에서는 1주단위의 강수량과 평균기온이 아닌, 4주간의 강수량합과 평균기온을 1주일 단위로 업데이트해 WINS에 제공하고 있다. 1주단위의 강수량과 평균기온을 취득하기 어려워, 평년 일단위 강수량과 평균기온 자료를 사용하여 4주간의 자료를 1주 단위로 분할하는 방법을 사용하였다. 주간단위 수문자료의 처리를 위해 국제표준기구(ISO)에서 제시하는 기준(ISO 8601)에 따랐다. ISO 8601은 월요일부터 일요일까지를 1주로 정의하며 현재 사용하고 있는 날짜체계와 1대1로 대응되도록 하였다. 예를 들면 1981년 2월 22일은 '1981-W07-7' 또는 '1981W077'로 표시한다. 표시된 형식은 1981년 7번째 주 일요일을 뜻한다. 이 기준에 따라 수문자료를 정리할 수 있도록 프로그램을 개발하였다. 주간 단위 잠재증발산량 계산은 월잠재증발산량 프로그램을 1주단위로 계산할 수 있도록 수정 및 보완하여 개발하였다. 수정 및 보완한 부분은 외기복사(外氣輻射)량 계산부분이다. 외기복사량은 지구가 태양을 1년 주기로 공전하므로 특정 위도에서 특정날짜에 따라 복사량이 달라지므로 주간단위의 월요일부터 일요일에 해당하는 날짜의 외기복사량을 각각 계산하고 이를 평균하여 주간단위 대푯값으로 사용하도록 하였다. 계산된 주간단위 외기복사량과 최고 최저기온을 입력하여 Hargreaves식에 의해 잠재증발산량을 계산한다. 융적설을 포함한 주단위 강우-유출 모형의 매개변수를 추정하기 위해 전국 24개 지점의 수문자료를 사용하였다. abcd 모형과 융적설모듈의 초기값 포함 11개 매개변수를 SCE-UA 전역최적화 알고리즘으로 추정하였다. 추정된 유역의 매개변수는 토양배수, 토양심도, 수문지질, 유역특성인자를 사용한 군집분석 결과에 의해 113개 중권역에 할당하였다. 개발된 주간단위 강우-유출 모형은 비교적 단기 가뭄전망을 위해 사용된다. 계산된 유량은 자연유량이며, 전국 취수장 수량, 하수처리장 방류수, 회귀수를 반영하여 지점별 유량을 계산하여 가뭄전망에 사용되고 있다.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2018.05a
• /
• pp.480-480
• /
• 2018

전 세계적으로 자연 친화, 하천생태계 보전, 친수하천 등을 조성하기 위한 대대적인 하천 정비사업이 활발히 진행 중에 있다. 최근 홍수로 인한 제방 붕괴에 대응하기 위한 제방의 안정화 및 개선을 위한 방법으로 기존의 시멘트와 같은 혼합물질을 사용하지 않고 환경 친화적이고 지속 가능한 대안에 대한 수요가 증가되고 있는 추세이며 현재 노후화 된 불안정 제방에 대한 보강대책을 수립해나가는 과정으로써 친환경 신소재를 활용하여 제방을 보호하는 연구가 수행되고 있다. 제방사면에 적용되는 신소재는 바이오폴리머를 활용한 재료로써 공동연구기관 카이스트에서 개발된 환경 친화적인 물질로 미생물에 의해 유도된 고인장 및 인체 무해성 등의 특성을 갖고 있으며 경제적 타당성인 측면에서 시멘트와 비교 분석 되어야 하고 실제 현장에서의 적용 가능성, 신뢰성 및 내구성 검토 등 성능을 보장하기 위한 지속적인 연구가 필요한 상황이다. 이에 본 안동하천실험센터에서는 중규모 제방을 직접 제작하여 수리모형실험을 통한 친환경 신소재 활용 제방의 안정성 및 성능 평가를 실시하였다. 수리실험 조건은 카이스트에서 제시된 레시피를 기반으로 먼저 분말형태의 바이이폴리머를 물과 희석하여 만들어진 바이오폴리머 용액을 흙과 혼합한 뒤 제방표면에 직접 미장작업을 수행하여 실험조건에 따라 일정한 두께(1cm, 3cm, 5cm)로 피복하였다. 이후 월류 붕괴 실험이 가능한 3 - 5일 정도의 양생기간을 거쳐 실험을 진행하였다. 실험결과는 다수의 고프로(GoPro) 및 비디오 카메라 등 다양한 영상장치를 이용하여 픽셀기반의 영상분석기법을 활용한 시간 흐름에 따른 제방 사면에서의 붕괴규모를 산정하여 신소재의 피복 두께에 따른 제체의 붕괴 거동 및 안정성을 평가하였으며, 또한 제방 파괴부에서의 흐름 상황 및 유속이 붕괴 발달에 미치는 영향을 분석하기 위하여 PIV 분석을 실시하였다. 이번 연구의 최종목표는 지속적인 예비실험을 수행하여 월류 및 침투, 파이핑 등 파괴 인자 별 신소재의 성능 개선 및 개발된 새로운 공법에 대한 효과 검토를 통한 최적안을 도출함으로써 향후 실규모 실험실증을 통한 신소재 시공 및 공법에 대한 현장적용 가능성 검증을 거쳐 최종적으로 신소재 제방 공법 설계 기술, 신소재 및 공법 표준안, 제방공법 안정성 평가 가이드라인 등을 제시하고자 하며, 이러한 실험데이터를 축적함으로써 실제 제방 붕괴 시 비상대처계획 수립에 필요한 기초자료로 활용이 가능할 것으로 사료된다.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.20 no.6
• /
• pp.768-773
• /
• 2014

In this study, experimental and numerical studies for the synthesis of carbon nanotube(CNT) in methane counterflow diffusion flame have been performed. Methane mixed with acetylene($C_2H_2$) was used as a fuel gas and ferrocene was used as a catalyst for synthesis of CNT. The major parameters was $C_2H_2$ mixing rate and mixing rates were 2 %, 6 %, and 10 %. Characteristics of CNT formation on grid were analyzed from SEM images. the chemical reaction mechanism adopted is GRI-MECH 3.0. Numerical results showed that flame temperature and CO mole fraction were increased with increasing acetylene mixing rate. Experimental results showed that the CNT synthesis in 2% acetylene mixture flame better than that of 6% and 10% acetylene mixture flames. It can be considered that 6% and 10% acetylene mixture flames generated the excessive carbon source and then it interrupted the supplement of the carbon source into ferrocene catalyst. It can be found that the supply of appropriate quantity of carbon source can make effect to synthesis of high purity of CNT.

• Journal of The Korean Society of Clinical Toxicology
• /
• v.8 no.2
• /
• pp.88-96
• /
• 2010

Purpose: Doxylamine is antihistamine drug that is used as a hypnotic. It is also used for suicidal attempts because it can be easily purchased at the pharmacy without a prescription. There were many articles about the complications after doxylamine intoxication such as a rhabdomyolysis, but only a few articles have reported on seizure. We reviewed the cases of doxylamine intoxication with seizure that were treated in the emergency department. Methods: We reviewed the medical records of the patients who were over 15 years old and who were intoxicated by doxylamine at 3 emergency medical centers from January 2006 to June 2010. We reviewed the patients' age, gender, the dose of doxylamine ingested, if gastrointestinal decontamination was done, the time from intoxication to hospital arrival, the seizure history, treatment of seizure, the electroencephalography (EEG) results, the brain computed tomography (CT) results and the blood test results. Results: There were 168 patients who were intoxicated by doxylamine during the study period. Twelve patients had a seizure episode. The differences between the patients who developed seizure and the patients who did not were the dose and the serum levels of sodium and creatinine. The only clinically meaningful difference was the amount of doxylamine. The amount of doxylamine ingested (>29 mg/kg) predicted the development of seizure with a sensitivity of 75% and a specificity of 92% on the ROC curve. One patient among the seizure patients expired in the emergency department. Conclusion: In case of doxylamine intoxicated patients, there is close relationship between seizure and ingested amount, so close observation needs to be done for the patients who ingest too much because doxylamine can cause death. Further prospective studies are needed for doxylamine intoxicated patients with a seizure episode.

• Korean Journal of Soil Science and Fertilizer
• /
• v.38 no.2
• /
• pp.66-71
• /
• 2005

Soil loss into stream and river by runoff shall be considered for non-point source pollution management as national land conservation. The purpose of this study was to develop the mathematical equation to predict soil loss from inclined uplands of maize cultivation due to the runoff by rainfall which mainly converges on July and August. Soil loss was concentrated on May because of low canopy over an entire field in 2002 and on June and July because of heavy rainfall in 2003. By regression analysis the relation between runoff and soil loss can be represented by a linear equation of y =1.5291x - 3.4933, where y is runoff ($Mg\;ha^{-1}$) and x is soil loss ($kg\;ha^{-1}$). The determination coefficient of this equation was 0.839 (P<0.001). Therefore, the mathematical equation derived from the practical experiment at the inclined upland can be applicable to predict soil loss accompanied by runoff due to periodic rainfall converging on short periods within a couple of months.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.6
• /
• pp.1035-1039
• /
• 2010

For 65 national forest areas in 1993 to 2008, the ambient sulfur dioxide ($SO_2$) concentrations were measured monthly using passive samplers and compared to the those of urban areas in order to investigate the characteristics of temporal and spatial distribution. In the forest areas, annual average concentration of sulfur dioxide gradually decreased from the beginning year of monitoring to 1997 and then had no significant change, such as the annual trend in urban areas monitored by Ministry of Environment. For the monitoring term, average concentration of sulfur dioxide in the forest areas was 5.6ppb, which was lower than the 10.1ppb in the urban areas and the EC ecological standard level (7.6 ppb). Seasonally, both in forest areas and urban areas the monthly average concentrations were much higher in winter and spring due to much more heating fuel consumption, and lowest in summer. Regional comparison to other regions of Gyeongbuk and Gyeonggi province showed that the concentration of sulfur dioxide was the highest during year. A significant positive correlation between sulfur oxides' emissions and sulfur dioxide concentration by province was observed, reflecting that the size and proximity of sources of atmospheric sulfur oxides could be important factors in determination.

• Journal of Sericultural and Entomological Science
• /
• v.50 no.2
• /
• pp.145-149
• /
• 2012

Cecropin is a well-studied antimicrobial peptide that play important role as key factor in insect humoral immunity. In this study, cecropin-like antimicrobial peptide was isolated and purified from the larval haemolymph of immune-challenged japanese oak silkworm, Antheraea yamamai. To isolate antimicrobial peptide, we separated and compared acidic extracted hemolymph protein bends between control and immune-challenged larvae using SDS-PAGE analysis. In the immune hemolymph extract, but not of non-immune hemolymph, we detected differential expressed peptide band with molecular mass 4,223.01 Da. To understand this peptide better, we successfully purified this peptide using cation exchange chromatography and gel permeation chromatography. Its N-terminal amino acid sequence obtained by Edman degradation evidenced a significant degree of identity with other lepidopteran cecropins. The purified A. yamamai cecropin-like peptide showed a broad spectrum of activity against fungi, Gram-negative and Gram-positive bacteria.

• The Journal of Korean Institute of Information Technology
• /
• v.15 no.12
• /
• pp.157-164
• /
• 2017

Mobile ECG signal measurement is a technique to measure small signals of several mV, and many studies have been conducted to remove noise including wandering scheme. Removal of the equipotential line noise caused by shaking or movement of the electrode cable is one of the core research contents for the electrocardiogram measurement. In this study, we proposed a modified step-size of combined NLMS(normalized least squares) and DLMS(delayed least squares) adaptive filter to eliminate baseline noise from ECG signals. The proposed method mainly adjusts initial filter step-size to reduce distortion of original ECG signals characteristic after eliminating baseline noise. The modified filter step-size is scaled by filter order size and distortion minimization factor. This method is suitable for portable ECG device with a small processor and less power consumption. This technique also decreases computation time which is essential for real-time filtering. The proposed filter also increase the signal to noise ratio (SNR) compared to conventional NLMS filter.