• Journal of Life Science
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• v.13 no.4
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• pp.516-521
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• 2003

Optimal conditions of protoplast isolation from the seaweed Prophyra pseudolinearis have been described. The P. pseudolinearis is one of indigenous and dominant Porphyra species in the East Sea region. Protoplasts have been released by enzymatic treatment of 2% agarase and 2% hemicellulase in 25mM MES buffer, pH 6.0 containing 0.5 M sorbitol. The protoplasts could be fused with neutral red-stained protoplasts of P. okamurae by the addition of polyethylene glycol 8000 solution.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.85-91
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• 2001

Biological treatment of benzene and toluene contaminated soil was investigated in laboratory microcosm of 16 different types for degrading benzene and toluene by indigenous bacteria. At the experimental conditions of the microcosms fast degrading benzene and toluene, moisture contents were 30% and 60% in a soil gap and content of powdered-activated carbon(PCA) for adhesion of benzene and toluene-degrading bacteria was 1% in total soil mass. At the conclusion of the shifted bacteria community, Case 6 and case 7 were operated until 10 days, and then the total cell number and the number of benzene and toluene degrading bacteria were investigated. The total cell number of Case 6 and Case 7 increased 488 fold and 308 fold of total indigenous cell, respectively. The number of benzene and toluene degrading bacteria increased and maintained the percentages occupied in pre-operating microcosm. Species of benzene and toluene degrading bacteria in microcosm changed from species of Gram negative bacteria to Gram positive bacterial species after soil exposed to benzene and toluene.

• Journal of Korean Society of Environmental Engineers
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• v.31 no.1
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• pp.35-41
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• 2009

This study was conducted to investigate feasibility of feedstuff for animal using food waste by fermentation mechanism of indigenous microorganism. To achieve this purpose, indigenous bacteria was isolated from soils to use as an inoculant. Enzyme test was performed to verify activity of amylase, protease and lipase using isolated bacteria. Bacteria(H1, D1), which vigorously express the enzyme activity, was selected and used in the fermentation experiments of food waste. From the analysis of 16s rDNA sequencing, H1 and D1 were identified as Bacillus subtilis and Paenibacillus polymyxa, respectively. In the fermentation experiment, food waste was mixed with rice bran and popped rice to control moisture and nutrient content. Isolated bacteria(B. subtilis and P. polymyxa) was used as an inoculant. From the measured data such as temperature, pH and ORP, it can be verified that food waste adding the indigenous bacteria was effectively fermented. From the nutritional analysis of manufactured feedstuff, it showed that the contents of crude protein, crude fat and crude fiber were enough to use as feedstuff for animal. In addition, harmful components such as Pb, Hg, Cd, aflatoxin and salmonella concentration were not exceeded permitted standards. Therefore, fermented food waste using indigenous bacteria can be used as feedstuff.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.1
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• pp.61-68
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• 1991

Thirty Bradyrhizobium japonicum isolates (10 strains per each soil) from 1 uncultivated [Sangnam(Soil 1), Milyang]- and 2 cultivated [Dong(Soil 2)and Chinbuk(Soil 3), Changweon] upland soils in Yeongnam area were evaluated on their symbiotic effectiveness to soybean [Glycin max (L.)] cv. Korean Jangbaekkong and American Clark and examined on their serological diversity. The results obtained were summarized as follows : 1. On symbiotic effectiveness of B. japonicum with plant genotypes, isolates showed a relatively high value of nodule mass in Jangbaekkong cv. and of shoot dry weight and total nitrogen in Clark cv. demonstrating the order of Soil 1> Soil 2> Soil 3 isolates. 2. Among 30 B. japonicum isolates, YCK 141 showed the best effectiveness on mean nitrogen fixation of two cultivars. 3. Thirty indigenous B. japonicum showed 6 types of serological diversities in the immunoblot analysis which were present in various proportions at Soil 2(5) and Soil 3(5) except Soil 1 where all isolates fell into the YCK 117 serogroup. And their distribution order was serotype YCK 117( 12 strains) > USDA 1l0(5strains), USDA 123(5 strains) > YCK 150(4 strains) > YCK 141(3 strains) > YCK 226(1 strain). 4. Especially, 10 isolates from Soil 1, an uncultivated orchard, showed a very homologous pattern in not only effectiveness but serological distribution. It seemed to indicate that the isolates were typically affected by numerous physical and environmental factors of the soil.

• Korean Journal of Poultry Science
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• v.41 no.1
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• pp.39-44
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• 2014

The goal of this study was to evaluate the growth performance of crossbred strains using Korean native chicken (KNC). The data were collected from 2012 through 2013 at Poultry Science Division, National Institute of Animal Science, Korea. The number of chicks analyzed in this study was 375. Crossbred were (A) $R{\times}S$, (B) $D{\times}H$, (C) $D{\times}S$, (D) $C{\times}Y$ and (E) $Y{\times}H$. The fertility rates of strains were 94.9% in crossbred A, 91.9% in crossbred B, 91.8% in crossbred C, 89.7% in crossbred D and 93.8% in crossbred E, respectively. The fertility was highest in crossbred A, but crossbred C showed the lowest (p<0.05) based on the hatchability. The crossbreds B and C exhibited a superior performance on body weight gains during growing stages but crossbred D showed the lowest (p<0.05). The feed intake was to the pattern of body weight. The feed intake of crossbred D was significantly lower than other crossbreds. Also, feed conversion ratio of crossbred D showed the lowest (p<0.05). Egg production ratio of crossbred D was significantly higher compared to the other crossbreds. These results suggest that the development of new crossbreds of commercial Korean Native Chickens should be required for better performance.

• The Journal of the Korea Contents Association
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• v.22 no.6
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• pp.629-637
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• 2022

It is at risk of depletion of biodiversity due to indiscriminate overfishing of ecosystems and destruction of habitats. Intensive fertilizers or development of related facilities to increase agricultural production in poor indigenous areas devastate the soil. Preservation of biodiversity is now emerging as an important issue of global human coexistence. After the Post-2020 GBF Declaration, all governance in agricultural development in indigenous agricultural areas should be supported and promoted as biodiversity conservation measures. A compromise plan to reduce ecosystem development and biodiversity loss can help establish public governance policies. In this paper, a viability kernel used for viable control feedback analysis is introduced to solve conflicting economic and ecological problems in ecosystem conservation, and a mathematical model on biodiversity conservation by the viability kernel is examined. Because all species in the ecosystem are interdependent, if the balance is broken, biodiversity is depleted, which is irreversible and eventually leads to extinction. For sustainable use and harmony of biological resources, a lot of policy consideration is required, such as creative governance that can efficiently protect all species. Subsidies or tax incentives have a direct impact on biodiversity conservation. The recovery of species in a state of decreasing biodiversity can be said to be of great economic value. Biodiversity will allow indigenous producers to be proud of their unique traditional knowledge and have a positive impact on local tourism, thereby enhancing regional identity and greatly contributing to the survival and prosperity of mankind.

• The Korean Journal of Mycology
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• v.48 no.4
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• pp.475-484
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• 2020

Various indigenous yeasts were isolated and obtained from flowers in the Republic of Korea, and their distribution and species diversity were studied. Seventy-seven flowers were collected from 25 areas in Korea, and 502 yeast strains were isolated from these flowers. A total of 50 species were identified by comparing large subunit rDNA gene sequence homology with the type strains of yeasts. The analysis of yeast distribution showed that the dominant yeast species were Aureobasidium pullulans, A. leucospermi, and Filobasidium magnum in each region and flower samples. Except for the above three yeast species, no species of yeasts showed any meaningful distribution among the habitat regions and sources. In conclusion, 50 species of indigenous yeasts were obtained from flowers that can be used as industrial resources, and the data could be used for further research on yeast diversity and interactions between yeast and its host.

• Applied Biological Chemistry
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• v.29 no.2
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• pp.175-181
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• 1986

Eightyseven strains of indigenous Rhizobia were isolated from the nodule of soybean cultivar, Danyup, cultivated in four different soils sampled from continuously soybean cultivated and newly reclaimed fields. The strains were grouped into Bradyrhizobium japanicum (slow-grower:55 strains) and Rhizobium fredii (fast-grower: 32 strains). The both groups could be divided into two sub-groups according to the denitrification characteristics, that is, denitrifying fast-grower (F-I), nitrate respiring fast-grower (F-II), denitrifying slow-grower (S-I). and nitrate respiring slow-grower (S-II). Among the 87 isolates, F-I, F-II, S-I and S-II sub-groups were 10, 22, 48 and 7 strains, respectively. The one-and two-dimensional polyacrylamide gel electrophoretic pattern of the four sub-groups were compared and discernible difference was observed between fast and slow-grower, but the difference was not discernible between subgroups within the same growth rate group.

• Korean Journal of Environment and Ecology
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• v.22 no.6
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• pp.648-657
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• 2008

This research was conducted to investigate the breeding density and seasonal food items of the magpies in Jeju Island and the near-manned islets. The examination of nest distribution to determine breeding density was performed during breeding season from February 2006 to April 2008, and that of food items from May 2006 to February 2008. A total of 2,113 nests were found across Jeju Island, the average density was $1.33\;nest/km^2$, and the magpies were distributed up to 600 meters above the sea level. The nest density was the highest in the central areas of Jeju Island, with 688 nests at $3.61\;nest/km^2$, while that in the eastern areas was the lowest, with 214 nests at $0.66\;nest/km^2$. In terms of the number of nests depending on the height above the sea level, 1,172 nests, which was equivalent to the density of $1.85\;nest/km^2$, was observed below 100m and highest among the intervals of height, but 16 nests found at 500-600m were the lowest, corresponding to $0.20\;nest/km^2$. The number of nests found in the manned islets near Jeju Island was eight in Biyang-do with the density of $15.38\;nest/km^2$, nine in U-do with $1.49\;nest/km^2$, and one in Gapa-do with $1.15\;nest/km^2$, whereas none of nests were observed in Mara-do. The contents of stomach consisted of 17 types of prey sources including countless bones, eggshells, plants, and seed, most of which were the individuals of the order Coleoptera. In spring and summer, the foraging frequency for invertebrate animals such as insects was high, but less than 30% in winter. In contrast, the magpies preyed upon plants and seeds at the frequency of 10% and 30%, respectively, in spring, while the foraging frequencies for both of them were 100% in winter and higher than any of other seasons. Eggshells and bones of birds were also detected infrequently. If the density of the magpies, which may play role as the upper predator within the ecosystem, increases, it would be expected to affect directly the reduction of the number of the species and population of endemic animals such as small birds and reptiles, etc. Therefore, it is considered that long-term monitoring for the density of the magpies and precaution is prerequisite to minimize adverse effects on ecosystem.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.277-285
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• 2001

This study was conducted to know about the distribution of naturalized plants growing in Jeiu Island. A field survey and flora lists of relevant literatures were also investigated. The results obtained from this study were as follows: Definition of naturalized plants was determined in this study as “non-native species in Korea which have been introduced anthropologically or naturally and can reproduce and survive in wild habitats by themselves.” It was suggested that the number of naturalized plants in Jeju Island were 31 families, 111 genera, 188 taxa including 178 species, 10 varieties. Family Compositae and Gramineae covered 23% and 14% of naturalized plants, respectively. The number of naturalized plants in Jeju Island were 188 taxa ; 97 taxa in the whole south Korea,39 taxa in the southern part of Korea,15 taxa in the central part of Korea, and 37 taxa only in Jeju.