• Title/Summary/Keyword: 토양발달

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Effects of Mineral Media, Carbon Sources and Phytohormones on Micropropagation of Alnus hirsuta (물오리나무(Alnus hirsuta)의 기내증식에 미치는 기본배지, 탄소원 및 식물호르몬의 영향)

  • 김경희
    • Journal of Plant Biology
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    • v.35 no.2
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    • pp.135-142
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    • 1992
  • Shoot tip explants from germinated seeds of Alnus hirsuta were cultured on NT (Nagata and Takebe, 1971) mineral salts medium supplemented with 6% glucose, MS (Murashige and Skoog, 1962) vitamin mixture, polyvinylpyrrolidone (PVP) and $0-50\;\mu\textrm{M}$ 6-benzylaminopurine (BAP). Five $\mu\textrm{M}$ BAP was found to give the highest shoot multiplication rate. Accordingly about 200 shoots were obtained for further experiments by multiplying shoots on this medium for 4-5 months. Regardless of carbon sources, NT mineral medium produced 3-12 times of shoots than MS mineral medium did. On NT mineral medium, 3% sucrose, 3% glucose and 6% glucose yielded no significant differences. It was observed that media consisting of 1/4-1/2 strength NT mineral salts, 3% sucrose and $1-8\;\mu\textrm{M}$ IBA produced about 100% rooting rate. Almost 100% of the resulting plantlets survived after transfer to the soil by decreasing humidity stepwise.epwise.

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A study on the Removal of Heavy Metals from Industrial Wastewater by Treatment with Discarded Automotive Tires (폐 타이어에 의한 고장폐수 내의 중금속 제거에 관한 연구)

  • Choung, Youn Kyoo;Min, Dal Ki;Oh, Hyun Je
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.6 no.4
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    • pp.29-42
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    • 1986
  • This study is an experimental research on the adsorption capacity and the adsorption system utilizing Discarded Automotive Tires(DAT) and Powdered or Granular Activated Carbon(PAC or GAC) for the removal of heavy metals, Ag(I), Cd(II), Cu(II), Zn(II). Batch shaking test was conducted to determine the adsorption capacity of DA T and PAC in removing the heavy metals from aqueous wastes; and laboratory-scale column experiment was performed to present design factors affecting the optimum design of adsorption column with DAT and GAC, through the concept of Bed Deph/Service Time(BDST). As results, DAT has been proven to be a good adsorbent will its adsorption capacity not falling behind PAC or GAC. Factors affecting heavy metals removals were amount of adsorbents, initial concentrations, pH and so on. BDST equations were compared with values presented by the breakthrough data from adsorption system.

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Modeling Study for Effects of Hydrothermal Clay Vein on Slope Stability (열수변질 점토맥이 사면 안정성에 미치는 영향에 관한 모델링 연구)

  • Jo, Hwan-Ju;Jo, Ho-Young;Jeong, Kyung-Mun
    • Economic and Environmental Geology
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    • v.43 no.2
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    • pp.185-196
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    • 2010
  • Clay veins that occurred in a slope by hydrothermal alteration, can significantly affect its slope stability. The effect of clay veins on the slope stability was investigated by numerical modeling study. Various parameters such as cohesion, internal friction angle, orientation, groundwater level, rainfall intensity and duration, have been modelled. As shear strength increased, factor of safety increased. As groundwater level developed, factor of safety decreased. For the case of slip surface developed on interface, factor of safety was lower than that for case of slip surface developed on either weathered soil or clay vein. The effect of various soil types of the slope stability was also investigated by simulating seepage through the slopes with various soils. The groundwater level significantly increased on the slopes with silty and generic soils. For the slope with sandy soil, almost no change in groundwater level was observed due to rapid drainage.

Genetic Transformation of Sweet Potato by Particle Bombardment (Particle Bombardment에 의한 고구마의 형질전환)

  • 민성란;정원중;이영복;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.5
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    • pp.329-333
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    • 1998
  • $\beta$-Glucuronidase (GUS) gene of Escherichia coli was introduced into sweet potato (Ipomoea batatas (L.) Lam.) cells by particle bombardment and expressed in the regenerated plants. Microprojectiles coated with DNA of a binary vector pBI121 carrying CaMV35S promoter-GUS gene fusion and a neomycin phosphotransferase gene as selection marker were bombarded on embryogenic calli which originated from shoot apical meristem-derived callus and transferred to Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxyacetic acid and 100 mg/L kanamycin. Bombarded calli were subcultured at 4 week intervals for six months. Kanamycin-resistant calli transferred to MS medium supplemented with 0.03 mg/L 2iP, 0.03 mg/L ABA, and 50 mg/L kanamycin gave rise to somatic embryos. Upon transfer to MS basal medium without kanamycin, they developed into plantlets. PCR and northern analyses of six regenerants transplanted to potting soil confirmed that the GUS gene was inserted into the genome of the six regenerated plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the vascular bundle and the epidermal layer of leaf, petiole, and tuberous root.

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In vitro Propagation of Transgenic Ginsengs Introduced with Ferritin Light Heavy Chain Gene through Single Embryo Culture (Ferritin Light Heavy Chain 유전자가 도입된 인삼형질전환체의 단일배발생을 통한 식물체의 기내증식)

  • 윤영상;김종학;김무성;양덕춘
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.161-168
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    • 2004
  • Optimal regeneration conditions of ginseng transformants were studied. It has been known that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal by combination, store and transport. To obtain the ginseng tolerant to heavy metal, binary vector was introduced in Agrobacterium by tri-parental mating and then Agrobacterium tumefaciens MP90/FLHC was selected on the AB media and MS media containing kanamycin. Explants were co-cultured with Agrobacterium tumefaciens MP90/FLHC, which contained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene and human ferritin light chain gene and then a number of embryos were induced. The induced embryo transferred to shooting media consisting of MS medium supplemented with GA 10 mg/L. As a result of examination that induced the normal growth of transfomants, transformants showed the equivalent growth in both root and shoot on the media containing the 1/3 MS.

Development of Semi-Distributed TOPMODEL (준분포형 TOPMODEL 개발)

  • Bae, Deg-Hyo;Kim, Jin-Hoon
    • Journal of Korea Water Resources Association
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    • v.38 no.10 s.159
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    • pp.895-906
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    • 2005
  • The diversity of observed hydrologic data and the development of geographic information system leads significant progress for developing distributed runoff models in the world. One of the typical examples is TOPMODEL, but the spatial coverage of its application Is limited on small headwater basins. The purpose of this study attempts to overcome its limitation and consequently develops a semi-distributed TOPMODEL. The developed model is composed of two components: a watershed runoff component for a lumped representation of hydrologic runoff process on the catchment scale and a kinematic wave type hydraulic channel routing component lot routing the catchment outflows. The application basin is the $2,703km^2$ upper Soyang dam site and several daily and hourly events are selected for model calibrations and verifications. The model parameters are estimated on 1990 daily event. The model performance on correlation coefficient between observed and computed flows are above 0.90 for the verification events. It is concluded that the developed model in this study can be used for flood analysis in large drainage basins.

Morphology of Silica bodies on Cyperaceae in Korea (한국산 사초과(Cyperaceae) 식물의 규소체(Silica body) 형태에 의한 분류)

  • Oh, Yong Cha
    • Korean Journal of Plant Taxonomy
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    • v.35 no.4
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    • pp.313-335
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    • 2005
  • Many plants take up soluble silica from the soil, and they deposit in plant tissues. Silica bodies are most commonly found in the leaf epidermis. Silica body is important factor in growth and development of plant. It help to maintain rigidity in stem and linear leaves, and its content may be correlated with resistance to fungal disease. In this study, several morphologically distinctive forms of silica bodies on Cyperaceae in Korea are recognized, which are eventually classified into two groups. Silica body type of first group (Rhynchospora, Fuirena, Scleria, Carex, Eriophorum) is A-type, which is conical in shape. Silica body type of second group (Cyperus, Scirpus, Eleocharis, Fimbristylis, Kyllinga, Bulbostylis, Lipocarpha) is B-type, which is conical in shape with numerous satellite body. These types are consistent enough to use as characters in taxonomic studies within genus. Silica body type may be associated with habitat: the species in dry region have A-type, whereas the species in wet region have B-type. These results suggested that shapes and presence or absence of silica body might be valuable to systematic analysis.

An Experimental Study on Water-Purification Properties of Concrete Using Effective Micro-Organisms (유용미생물을 이용한 콘크리트의 수질정화특성에 관한 실험적 연구)

  • Seo, Dae-Seuk;Jeon, Jin-yong;Kim, Jeong-Hwan;Kwon, Hyuk-Jun;Park, Seung-Bum
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.757-760
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    • 2008
  • Recently, improvements in the standard of living in industrial area require establishment of a convenient residential environment in order to enhance the quality of living, To achieve such an environment, it is necessary to effectively reduce or prevent various environmental problems occurring in and around residential areas. In this study, We focused on the water-purification properties of concrete using effective micro-organisms. Such as SS, BOD, COD, T-P, T-N. The ability of the removal of SS, T-P, T-N in the test water is superior to concrete using effective micro-organisms. As a result, concrete using effective micro-organisms has sufficient performance of water-purification.

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Application of Geophysical Prospecting to Site Assessment of Waste Landfill (매립지 오염평가를 위한 물리탐사의 적용사례)

  • Lee, Cheol-Hyo;Park, Sam-Gyu
    • 한국지구물리탐사학회:학술대회논문집
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    • 2001.09a
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    • pp.104-121
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    • 2001
  • Recently, the pollution of soil and groundwater becomes a serious social problem, and geophysical exploration methods have been introduced as a remedial investigation method of subsurface. Digital technologies such as personal computer have revolutionized our ability to acquire large volume of data in a short term, and to produce more reliable results for subsurface image. Also, color graphics easily visualizes the survey results in a more understandable manner, and it is widely used for not only characterizing the contaminated subsurface but also monitoring contaminant and remedial process. In this paper, electrical resistivity and electromagnetic (EM) surveys were carried out in order to understand characteristics of waste landfills, and the applicability of geophysical prospecting to site assessment of waste landfill was also tested. According to the result, electrical resistivity and electromagnetic (EM) surveys were effective in estimating distribution of the leachate plume.

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Moor Vegetation of Mt. Shinbul in Yangsan (양산 신불산의 습원 식생)

  • Kim, Jong-Won;Han, Seung-Uk
    • The Korean Journal of Ecology
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    • v.28 no.2
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    • pp.85-92
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    • 2005
  • This study emphasizes syntaxonomy and syndynamics of intermediate (Zwischen) moor (area: 14,000 $m^2$) at Mt. Shinbul in Yangsan, southeastern Korean Peninsula. A total of 105 vascular plant species including 26 monitor-species were recorded. Analysis by the $Z\"{u}rich$-Montpellier School's method distinguished eight vegetation units: Eleocharitis-Blyxetum echinospermae ass. nov., Eriocaulon sikokianum-Utricularia racemosa community, Eleocharis wichurai-Molinia japonica community, Platanthero-Molinietum japonicas, Miscanthus sinensis for. purpurascens community, Tripterygium regelii community, Symplocos chinensis-Quercus mongolica community, Symplocos chinensis-Quercus dentata community. PCoA (Principal Coordinates Analysis) shows that vegetation changes and distributional aspects are associated with both moisture condition and sunlight on the ground layer and soil nutrient level (mesotrophic to oligotrophic). Most important to Molinietea japonicas being representative intermediate moor vegetation at the southeasternmost fringe of the Korean Peninsula is the local cooling effect by mountainous cloud and mist zone resulting in shorter and wetter growing season. The Yangsan moor vegetation was compared with earlier descriptions of related Mujechi moor from anthropogenic and natural moor vegetations.