• Journal of Korean Society of Forest Science
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• v.78 no.3
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• pp.302-313
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• 1989

To investigate the effects of forest fire on the change of vegetation and soil conditions by the lapse of time, the 5th soil survey and vegetation analysis were carried out in July of 1988 at the rocky areas of Mt. Kwanak, where ground fire broke out 5 years ago. Three-year increasing trends of organic matter content, the value of pH, and total nitrogen content stabilized after the restoration of the vegetation. By the comparison of the occurrences of the species for 5 years after the forest fire, woody and herbaceous plants were categorized into 4 groups, i,e., invasive, sensitive, tolerant, and neutral species to the forest fire. Although such diversity indices as the species diversity and similarity index indicate that the restoration of damaged vegetation to the original species composition was proceeded for the period, the restoration of vegetation to the original crown closure and tree growth was not proceeded yet.

• Spatial Information Research
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• v.3 no.1
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• pp.1-18
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• 1995

Despite the widespread use of GIS over the past ten years, it has been limited application for regional modeling of pollutant loadings such as sediment, nitrogen, and phosphorus(non-point source pollution), The goals of this study were to: select important processes and parameters of watersheds that contribute to non-point source pollution degradation, develop a ranking model to use the environmental geologic data and verify the model by comparing results with existing water quality data(Chung-ju Lake) for specific watersheds, The GIS database consisted of topography, geology, soils, precipitation, rainfall erosivity, land use, and watershed boundaries. The index(NPSP) for assessing non-point source pollution was comprised in the following three seperate components: soil loss index(SLI) assesses the potential soil erosion and sedim-ent delivery from field to stream; run-off potential ratio(R.P.R) predicts the potential production of surface runoff; chloropgyll-a index ranks the potential manure(animal or human) production within a watershed. The GIS model was a valuable tool to assess the impact of environmental pollation in watersheds.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.395-403
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• 2011

This study was conducted to investigate the habitat positional environment and vegetation properties of Mankyua chejuense. The habitats were classified into two types depending on the depth of the habitat, the ratio of rock exposure, the dominant species and the surrounding vegetation, which affected the vegetation of the habitats. The habitats with a high ratio of rock exposure showed a distinctive geographical boundary to the adjacent region and most of them were composed of trees that grow in humid environment. On the contrary, in the soil-rich habitats, the depth was shallow, soil layer was well developed, and the trees were introduced from the adjacent areas. However, the dominant species in the herbaceous layer were aquatic plants, which indicated that the habitats had the properties of wetland. Therefore, it was found that thes habitats of Mankyua chejuense have the properties similar to those of marshland. For the preservation of Mankyua chejuense, it is very important to make the habitats maintain such properties of wetland.

• Journal of Environmental Health Sciences
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• v.29 no.2
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• pp.7-15
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• 2003

Di-2-ethylhexyl phthalate (DEHP), is a widely used plasticizer known to be a suspected endocrine disrupter, but its exact effects on aquatic organisms are not yet known. When Japanese medaka (Oryzias latipes) were exposed from the time of hatching to 3 months of age to an aqueous DEHP solution at nominal concentrations of 1, 10, and 50 $\mu\textrm{g}$/l, DEHP treated female fish showed distinct reproductive effect. And the midge (Chironomus riparius.). an aquatic invertebrate, was exposed to DEHP to evaluate the effects on reproductive processes via sediment toxicity. The test endpoints included emergence, sex ratio, fecundity, and the viability of F1 offspring egg ropes. The result implied that the normal developmental and/or reproductive processes in C. riparius had been disrupted when exposed to DEHP, the effect also being displayed in the next generation. In summary, DEHP hinders the development of reproductive organs in the female Japanese medaka and C. riparius.

• Korean Journal of Medicinal Crop Science
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• v.13 no.1
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• pp.57-62
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• 2005

Salt-tolerant transgenic Panax ginseng plants were produced by introducing the SAL1 geue (3'(2'), 5'-bis-phosphate nucleotidase) that confers tolerance to the salts through Agrobacterium tumefaciens co-cultivation. Cotyledon explants of immature ginseng zygotic embryos cultured on Murashige and Skoog medium lacking growth regulators formed somatic embryos directly with below 10%, but the 74% tranformation rate were observed at the treatment of phytohormone with 1.0 mg/l 2,4-D and 0.5 mg/l kinetin. Somatic embryos were initially cultured on MS medium supplemented with 250 mg/l cefotaxime for 3 weeks and subsequently subcultured five times to a medium containing 100 mg/l kanamycin and 250 mg/l cefotaxime. Upon development into the cotyledonary stage, these somatic embryos were transferred to on the medium containing 50 mg/l kanamycin and 10 mg/l gibberellic acid to induce germination and strong selection. Integration of the transgene into the plants was confirmed by polymerase chain reaction with specific primers. The ginseng transformants with well-developed shoots and roots were successfully acclimatized in a greenhouse when they were planted in soil.

• Horticultural Science & Technology
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• v.35 no.1
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• pp.46-58
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• 2017

This study was conducted to investigate the optimal conditions for spore germination, prothallus propagation, sporophyte formation and seedling growth in Microlepia strigosa (Thunb.) C. Presl. Spore germination and prothallus development were promoted by low concentrations of Knop medium nutrient solution. The optimal medium for prothallus propagation and antheridium formation was 2X MS medium with 3% sucrose. The activated charcoal content of the medium did not affect prothallus proliferation. Among the various combinations of culture soil (bedding soil, peat moss, perlite and decomposed granite), a mixture of bedding soil, peat moss and decomposed granite at a ratio of 1 : 1 : 1 (v : v : v) had a positive effect on sporophyte formation. The most efficient conditions for promoting the growth of whole plants (sporophyte seedlings) were 50 - cell plug trays filled with a mixture of bedding soil and decomposed granite at a 2 : 1 (v : v) ratio.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.263-268
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• 1997

Effects of plant growth regulators and condition on shoot induction from internode explants of Actinidia arguta were investigated. Optimal condition for shoot induction was obtained when internode explants were cultured on MS medium containing 3.0 mg/L zeatin. The frequency showed about 62.5% under darks condition at 27$^{\circ}C$ after 6 weeks of culture. Regenerated shoots were rooted on WPM medium supplemented with 0.5 mg/L NAA and the regenerants were acclimated to an artificial soil with mixture (perlite : vermiculite = 1:1, v/v) for further growth and successfully transplanted to the soil in a highly humidified greenhouse. Histological observations revealed that meristemoids were formed from small and isodiametric cells, and then developed to shoot primordia.

• Journal of Forest and Environmental Science
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• v.10 no.1
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• pp.8-24
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• 1994

Because of the underground existence of roots, a few studies have been reported on root system. The developmental information of roots should be understood for the studies of specific tree traits and the influence of such traits on the soil surface fixation. In order to clarify the specific character of pine forest in Kangwon Province, the investigation on the form and distribution of root system of pine trees were carried out for 5 trees in the Campus Forest, Kangwon National Univ.. Root form was very well in flat root. As soil depth was approximatly 50cm, fine roots were very sparsly distributed(+), roots of 0.2cm in diameter were most common and roots > 0.2cm were very rare, also thickness thined. 60~70% all the roots were developed at the depth of 0~30cm, where big roots were below 0.9cm in diameter and fine roots were higly sparse(+).

• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.185-188
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• 2002

Off-white, friable embryogenic calluses were formed on the internal integument of mature seeds of yuzu (Citrus junos) cultured on Murashige and Skoog's basal medium at a frequency of 1.2%. Embryogenic calluses were proliferated when cultured on medium with 1 mg/L 2,4-D. Upon transfer to medium with 0.1 mg/L kinetin, embryogenic calluses produced numerous somatic embryos. Embryogenic suspension cultures were established by placing embryogenic calluses into liquid medium with 1 mg/L 2,4-D. When plated onto medium with 0.5 mg/L ABA, embryogenic cells developed into somatic embryos at a high frequency, and then regenerated into plantlets. Plantlets were successfully transplanted to potting soil and grown in a greenhouse.

• Korean Journal of Organic Agriculture
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• v.18 no.2
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• pp.245-255
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• 2010

The study was conducted to investigate the effects of organic fertilizers and mulches on the growth and $CO_2$ assimilation in MM.106 apple trees. Growth and $CO_2$ assimilation of MM.106 apple trees grown in a greenhouse were affected by the nutrient concentrations and carbon (C) and nitrogen (N) ratio in the raw materials of organic fertilizers and mulches. The optimum C:N ratios, which makes microorganism convert the organic N into the inorganic N, were obtained in the organic fertilizer, poultry litter, green compost, and grass clippings, resulting in increasing single shoot height, SPAD, and $CO_2$ assimilation. The SPAD and $CO_2$ assimilation were affected by the treatments 5 weeks after the treatments, and then the tree growth was affected by the treatments 6 weeks later. The most efficient tree growth and development were observed in the 10 to $15\;mg{\cdot}kg^{-1}$ of the inorganic N in a soil, and the N was strongly related to the tree growth and development.