• Title/Summary/Keyword: 토양대사

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Establishment Aerobic Soil Metabolism System Using [14C]Butachlor ([14C]Butachlor를 이용한 호기성 토양대사 시험법 확립)

  • Kim, Ju-Hye;Kim, Jong-Hwan;Kim, Dae-Wook;Lee, Bong-Jae;Kim, Chan Sub;Ihm, Yangbin;Seo, Jong-Su
    • The Korean Journal of Pesticide Science
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    • v.18 no.4
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    • pp.258-268
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    • 2014
  • The test method of aerobic transformation in soil has established based on international test guideline (OECD TG 307). And then, the case study was conducted with [$^{14}C$]butachlor. Butachlor is commonly used herbicide in Korea. [$^{14}C$]Butachlor was treatrd $6.83mgKg^{-1}$ in loamy soil. The treated soil was incubated in flow-through system for 60 days. The mass balance of applied radioactivity (AR) ranged from 91.1 to 95.5% and from 93.0% to 97.7% for non-sterile and sterile soils, respectively. In non-sterile soil, the concentration of [$^{14}C$]butachlor was declined from 94.4% AR at 0 day to 8.4% AR at 60 days after treatment. 2-Chloro-2',6'-diethylacetanilide was the major degradation product detected in soil extract. The calculated $DT_{50}$ and $DT_{90}$ of butachlor were 10.4 days and 34.6 days, respectively. $^{14}CO_2$ and non-extractable soil residue were increased up to 3.5% and 43.5% AR at 60 DAT. There is no significant decrease of the [$^{14}C$]butachlor through the incubation period in sterile soil.

Patterns of Utilizing Sole Carbon Source by Soil Microbes in a Forest Soil (토양 세균 군집의 유일탄소원 이용에 의한 지문분석)

  • 송인근;최영길;안영범;신규철;조홍범
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.65-71
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    • 1999
  • This study was carried out utilzing ability of sole carbon sources in soil microbial communities used by Biolog GN microplate. Cluster analysis showed that soil microbial cornmuties were categorized into three groups as forest, non-forest soil and naked soil of microbial group. Soil microbial commutites in a forest soil of Qirercus mongoIica was divided into another group microbial communites in Qirercus dendata vegetation soil and Pinus dnzsqlora vegetation soil by Multidimensional scaling(MDS). Generally, sole carbon utilzing abilties were higher in order of polymer, amino acids and carboxylic acids, but it was lower in amides substrates carbon group. From the result: it was supposed that metabolic diversity of microbial communities was corresponded to vegetation succession.

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Influence of Starvation and Humic Acid on Soil Microbial 2- Hydroxypyridine Metabolism (토양 미생물의 2-hydroxypyridine 대사에 미치는 기아상태와 부식산의 영향)

  • 황선형
    • Journal of Korea Soil Environment Society
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    • v.4 no.1
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    • pp.13-23
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    • 1999
  • In this research, 3-hydroxypyridine(2-HP) metabolic ability of starving Arthrobacter crystallopoietes cell and the effect of humic acid on the metabolism of this starving cell were evaluated. 2-HP metabolic ability of exponential phase cell (acclimated cell) was much higher than that of lag phase cell (unacclimated cell) during starvation period. After 3 days of starvation, 2-HP half-life of the acclimated cell was 14 hours and that of the unacclimated cell was 46.5 hours. Humic acid enhanced the stability of 2-HP monooxygenase of starving co]1 and, after 2 days of starvation, the residual activity rate of this enzyme of the microbial cell starved in humic acid solution was 12% while the rate for control condition was 1.5%. After 14 days of starvation, 2-HP half-life for control condition was 43 hours and that for humic acid condition was 1.25 hour.

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Uptake of Soilmicrobial Metabolites and Allelochemicals in Plant Root System (식물의 뿌리에 의한 토양 미생물 대사산물 및 Allelochemicals의 흡수)

  • Kim, M.J.;Lee, Y.S.
    • Korean Journal of Environmental Agriculture
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    • v.16 no.3
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    • pp.264-268
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    • 1997
  • Microbial metabolites from rhizosphere soil samples mainly inhabitated by Streptomyces are selectively uptaken into plants. The culture broth of a Streptomyces strain K9301 showed a major metabolite which disappeared in the medium 24hrs after planting of seedlings. This metabolite was selectively uptaken in the rice plants as well as the wheat plants. We identified the targeted metabolite showing a strong UV-absorbing spot at Rf 0.6 on TLC to be 2-aminobenzamide.

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신대사길항물질 plumbemycin A 및 B에 관한 연구

  • 박부길
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1977.10a
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    • pp.193-194
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    • 1977
  • 대사길항물질에 의한 미생물의 생육조해는 그 시험계에 대사검질을 첨가하므로써 회복되는 것이며 최소검정배지에 대한 대사조해에 대사물질, 예로 amino산, 핵산등을 첨가해서 조해회복을 screening하므로써 그들 물질(amino산 핵산등)의 대사에 길항하는 새로운 화합물을 발견할수가 있다. 이러한 견지에서 고선균이 생산하는 새로운 amino 산대사 길항물질이 탐색을 목적으로하여 screening한 결과 토양에서 새로이 분이한 Streptomyces 1주의 탑양액중에 L-threonine에만 결항작용을 나타내는 물질의 존재를 확인하고 유사구조를 갖는 2종의 tripeptide 대사조항물질의 단이에 성공하였으며 이들을 plumbemycin A 및 B라고 명명하였다.(중략)

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Degradation of Diazinon and Dursban in Submerged Soil (담수양중(湛水壤中) Diazinon 과 Dursban 의 분해(分解)에 관(關)하여)

  • Choi, Jong-Woo;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.6 no.2
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    • pp.1-11
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    • 1987
  • The degradation of two chemicals seem to be clearly affected by soil microbial activity in submerged soil $conditions(30{\pm}1^{\circ}C)$. The Active ingredient of Diazinon disappeared about 5 times faster than that of Dursban. By Applying 300% higher concentrations of both chemicals. under the above soil conditions, however, degradation was retarded by about one day. Some of the metabolites of Diazinon were as follows: 0.0-diethyl phosphorothioate and sulfotep as hydrolytic products, and diazoxon, 0.0-diethyl-0-[2-(1-hydroxy-1, 1-dimethyl)-6-methyl]-pyrimidinyl phosphorothioate and 2-isopropyl-6-methyl-pyrimidine-4-one as degradation products of monooxygenase. But 0. 0-diethyl phosphorothioate was the only methabolite of Dursban.

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Degradation and Metabolism of Phorate in Soil (토양중(토양중) Phorate의 분해(분해)와 대사(대사))

  • Lee, Hae-Keun;Hong, Jong-Uck
    • Applied Biological Chemistry
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    • v.26 no.2
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    • pp.97-103
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    • 1983
  • To get further information on the behavior of phorate(0,0-diethyl S-ethylthiomethyl phosphorodithioate) in soil under the subtropical conditions, a field experiment has been conducted. Phorate granule (10%) was applied to silt loam soil at the rate of 40kg a.i./ha and incorporated to 10cm soil depth. Residues of phorate and its metabolites in soil were determined with GLC and confirmed qualitatively with TLC. Phorate was rapidly oxidized to its sulfoxide and sulfone. Therefore, main metabolic pathway of phorate in soil was the oxidation of phorate to phorate sulfoxide and sulfone. Phorate sulfoxide and sulfone were relatively more persistent than phorate itself. Phoratoxon was detected at low level only up to 30 days after treatment and its sulfoxide and sulfone were not detected during the whole experimental period. Toluene-acetonitrile-nitromethane(40 : 30 : 30, v/v/v) solvent system separated satisfactorily phorate and its five metabolites. Most of the residues was found in the initial incorporation depth $(0{\sim}10cm)$. Consequently, insecticides showed a little downward movement.

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Comparison of metabolic diversity by sole carbon source utilization and genetic diversity by restriction patterns of amplified 16S rDNA (ARDRA)in soil bacterial communities. (토양세균 군집의 대사 다양성과 16S rDNA의 제한효소 지문분석에 의한 유전적 다양성의 비교)

  • 송인근;최영길;김유영;조홍범
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.72-77
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    • 1999
  • To investigate soil bacterial diversity according to vegelalioo types, utilizing ability of sole carbon sources and restriction enzyme patterns of 16s rDNA were analyzed. From the both results; five kinds of soil microbial communities were grouped as forest soil (Quercus mongolica and Pinus densi&ra vegetation), grass-agricultured soil and microbial communities of naked soil. But, both soil microbial communities of directily exlracted from ths soil and indirectly extracted from heterotrophic bacteria that cultured soil in LB medium showed very different similarity.

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