• Korean Journal of Oriental Medicine
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• v.6 no.1
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• pp.99-105
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• 2000

Lithium, a simple monovalent cation, is the mainstay in the treatment of manic-depressive illness, but despite extensive research, its mechanism of action remains to be elucidated. Because lithium requires chronic administration for therapeutic efficacy and because its beneficial effects last well beyond its discontinuation, it has been postulated that lithium may exert major effects at the genomic level. We have previously shown that Yungmijihwangwon (YM) increase TH protein expression through antioxidant activity and unknown effects in vivo. In the pressent study, we have sought to determine if lithium in herbal medicine also increases the expression of endogenous gene known to be regulated by AP-1 and have therefore investigated the effects of herbal medicine on tyrosine hydroxylase (TH) levels. Male mice were treated with LiCl and herbal medicine for 30 days, and TH levels and dopamine level were measured in striatum using immunoblotting and ECD-HPLC. Herbal medicine treatment resulted in statistically nonsignificant increase in TH levels in mouse striatum. The precise therapeutic relevance of these effects is presently unknown.

• Journal of Sericultural and Entomological Science
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• v.45 no.1
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• pp.46-57
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• 2003

This study was carried out to investigate the characteristics of the soluble sericins after degumming and after hydrolysis of insoluble sericin with various enzymes. Especially, the hydrolysis characteristics were examined in terms of molecular weight of the soluble sericin. Amino acid composition and molecular weight characteristics of the soluble sericins were also studied. When the insoluble sericin was hydrolyzed with kojizyme and flavourzyme, the solubility was highest at pH 7 and 50$^{\circ}C$. On the other hand, in the cases of protamex and alcalase, the highest solubility was obtained at 60$^{\circ}C$. In these cases, solubility increased with pH. In enzymatic hydrolysis, the solubility was increased with concentration of enzymes until 4 hours. After then, a slight difference was found along with treatment times. In enzymatic hydrolysis, the absorbance of the soluble sericin was increased with concentration of enzymes and treatment times. Average degree of polymerization was decreased with treatment time and concentration. The amino acid compositions were similar in low(low molecular weight by degumming) and high (high molecular weight by degumming). Those of PK (soluble sericin hydrolyzed with kojizyme), PP (soluble sericin hydrolyzed with protamex), and PA(soluble sericin hydrolyzed with alcalase) were similar to each other. Serine and tyrosine compositions were higher in low and high than those of PK, PP, and PA. However proline was absent in low and high. Molecular weights of the various sericins became higher as KP>high>PP>low>PA and those of KP and PA were 9,800 and 905 respectively.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2004.11a
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• pp.42-49
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• 2004

본 연구는 선천선고혈압쥐(SHR)에서와 마찬가지로, 높은 정상혈압 및 중등 정도의 본태성고혈압 환자에서 Valyl-Tyrosine(VY)의 항고혈압 효과 여부를 결정하기 위해 수행되었다. 29명의 지원자에 대하여 무작위 이중맹검 위약검사를 실시하였으며, 3 mg의 VY를 포함하는 100-ml 음료와 100ml-위약 음료를 조제하여 사용하였다. 연구대상은 VY (남16/여1, 45.5${\pm}$3.2세, 146.4${\pm}$2.3/90.5${\pm}$1.8 mmHg) 집단과 위약(P) (남11/여1, 48.8${\pm}$3.0 세, 145.5${\pm}$2.4/92.3${\pm}$1.8 mmHg) 집단으로 나누었다. 대조(C)기간 3주 째에 VY-음료 또는 P-음료를 하루 2번씩 투여하기 시작하여 4주 간의 실험(E)기간(BP)은 매주 측정되었으며, 아침에 앉은 자세에서 측정하였다. 혈액 표본은 C기간과 E기간의 마지막 날 채취하였다. VY집단에서, 수축기(S) 및 이완기(D) 혈압의 감소는 첫째 주에 각각 9.7 및 5.3 mmHg (P < 0.001)이었으며, E기간의 시작에 따른 넷째 주에는 각각 9.3 및 5.2 mmHg (P < 0.001)이었다. P 집단에서는 SBP와 DBP 모두 변하지 않았다. VY 집단의 혈압은 회복기간의 끝까지 점진적으로 증가하였다. VY집단에서, 혈장 안지오텐신(Ang) I과 VY의 농도는 현저히 증가한 반면 Ang II와 알도스테론은 VY 투여 후에 현저히 감소하였다. VY는 SHR에서와 마찬가지로 중등 정도의 고혈압 환자에서도 Ang I-전환효소 억제를 통하여 현저한 항고혈압 효과를 가지는 것으로 보이며, 어떤 부작용도 검출되지 않았다.