• Journal of Korean Society of Forest Science
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• v.100 no.4
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• pp.715-721
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• 2011

This study was conducted to estimate aboveground biomass and nitrogen storage potential of tree species-eight clones of a poplar and hybrids, one clone of Salix alba L., dawn redwood (Metasequoia glyptostroboides Hu and W.C. Cheng), yellow poplar (Liriodendron tulipifera L.), Okamoto maple (Acer okamotoanum Nakai), and pin oak (Quercus palustris $M{\ddot{u}}nchh.$)- after treating with liquid pig manure. Stems showed the highest percentage of aboveground biomass, and followed by branches and leaves. Nitrogen content in aboveground biomass components was the highest in leaves, and followed by branches and stems. Average aboveground biomass production was higher in the clones and species treated with manure than those of not treated, 30 ton/ha and 16 ton/ha, respectively. In the manure-treated site, clone 'Dorskamp' of Populus deltoides${\times}$Populus nigra showed the highest aboveground biomass (48.3 ton/ha). Average nitrogen storage potential was superior in the clones and species treated with manure than those of not treated, 159 kg/ha and 90 kg/ha, respectively. Clone 'Dorskamp' also showed the greatest nitrogen storage potential (286.5 kg/ha) among tested tree species. Therefore, 'Dorskamp' is the most suitable clone for treating liquid pig manure, but additional studies are needed to determine any damages or tolerance from the treatment.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.15 no.1
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• pp.25-35
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• 2010

The biodiversity of eukaryotic plankton has commonly been used to evaluate the status of aquatic ecosystems. Therefore, an accurate and rapid method for species identification is needed to reveal the biodiversity of environmental water samples. To date, molecular methods have provided a great deal of information that has enabled identification of the hidden biodiversity in environmental samples. In this study, we utilized environmental polymerase chain reaction (PCR) and constructed the 18S nuclear ribosomal RNA clone library from environmental water samples in order to develop more efficient methods for species identification. For the molecular analysis, water samples were collected from the Seonakdong River (Gimhae Bridge) and the coast of Namhae，(Namhaedo). Colony PCR and restriction fragment length polymorphism of PCR (PCR-RFLP) were then adopted to isolate unique clones from the 18S rDNA clone library. Restriction fragment length polymorphism pattern analysis of the Gimhae Bridge sample revealed 44 unique clones from a total of 60 randomly selected clones, while analysis of the Namhae sample revealed 27 unique clones from 150 clones selected at random. A BLAST search and subsequent phylogenetic analysis conducted using the sequences of these clones revealed hidden biodiversity containing a wide range of taxonomic groups (Heterokontophyta (7), Ciliophora (23), Dinophyta (1), Chytridiomycota (1), Rotifera (1) and Arthropoda (11) in the Gimhae Bridge samples Ciliophora (4), Dinophyta (3), Cryptophyta (1), Arthropoda (19) in the Namhae samples). Therefore, the molecular monitoring method developed here can provide additional information regarding the biodiversity and community structure of eukaryotic plankton in environmental samples and helps construct a useful database of biodiversity for aquatic ecosystems.

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.6-12
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• 1990

The preS2 wequence of an adr hepatitis B virus was cloned and expressed in Escherichia coli as a $\beta$-galactosidase fusion polypeptide. Recombinant preS2 product interacted with the preS2-specific monoclonal antibody H8 which was induced by surface antigen particles isolated from a Korean gepatitis patient. The H8 showed only a minor cross-reactivity with recombinant preS2 product of adw2 subtype. Determination of nucleotide sequence of the adr preS2 revealed that twelve amino acid residue substitutions between adr and adw2 subtype sequences. The antigenic determinant to H8 must include some of these differences.

• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.113-116
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• 2001

Chromosomal variation was investigated in the female and male somaclones regenerated from the leaf segment culture of Rumex acetosa L. Difference in phenotype depending on the sexuality was not observed. In female somaclones, 21 among 25 somaclones carried the same chromosome complements (2n＝14) with wildtype and others were tetraploids (2n=28), Considerable chromosomal variation was found in male somaclones. Only 4 among 20 somaclones carried normal chromosome number (2n=15) and 13 somaclones were aneuploids.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.371-374
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• 2012

본 연구는 "사이클론과 관성충돌 및 백필터의 제진원리를 일체화한 고효율 멀티 제진시스템의 최적화 설계를 위한 수치 해석적 연구(I) : 집진기 입구 최적화 설계"에 이은 일련의 연구로서 앞선 연구에서 도출된 사이클론부 하단 벤츄리(Venturi)에서의 강한 하향 기류의 가속에 따른 분진 재비산 가능성에 대한 추가적 검토를 위한 연구이다. 사이클론부를 통과한 기류가 상향 흐름으로 방향을 전환할때 좀 더 가속시켜 빠르게 곡률반경을 형성하여 조대 입자의 분리를 극대화시키기 위한 목적으로 사이클론부 하단을 벤츄리 형상으로 설계하였으나, 유동장 분석 결과 벤츄리를 통과한 처리가스 흐름이 가속되면서 호퍼 하단까지 약 4~5 m/s의 강한 하향 흐름을 형성하고 호퍼 하부의 말단 부근에서 상향 흐름으로 방향 전환을 하고 있는 것으로 예측됨에 따라 설계 의도와는 달리 벤츄리 설치시 호퍼 하단에 포집된 분진의 재비산을 예방하는데는 크게 역할을 하지 못하는 것으로 나타났다.

• Proceedings of the KAIS Fall Conference
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• 2012.05a
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• pp.367-370
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• 2012

본 연구는 하나의 장치 내에서 "싸이클론-관성충돌-여과포 집진"이라는 다단 제진 단계가 동시에 이루어져 입경 분포별로 순차적인 제진이 가능한 멀티 집진기의 처리가스 제어 효율을 극대화시킬 수 있는 기류 흐름을 얻기 위한 최적화 설계 조건을 검토하는 것이다. 우선 1단계 제진 과정인 원심력을 이용하여 조대입자의 유선 이탈을 촉진시킬 수 있는 사이클론 유동을 최적화시키기 위한 집진기 입구 형상 설계 검토를 위해 수치 해석적 연구를 수행하였다. 그 결과, 멀티집진기 입구 형상을 일반적인 설계 방식인 접선 유입식으로만 설계한 것에 비해 선회류를 한번 더 가속화시킬 수 있는 가이드 베인을 추가로 설계한 경우 선회류의 pitch가 짧고 강하게 형성되어 사이클론부에서 조대입자의 유선 이탈을 촉진시키는데 매우 효과적인 것으로 예측되었다. 단, 사이클론부 하단 벤츄리 형상으로 인해 약 4~5 m/s의 강한 하향 흐름이 호퍼 하단까지 형성되고 있어 탈리 분진의 재비산 문제가 발생할 가능성이 크므로 벤츄리 형상 설치 유무에 대한 영향도 추가적으로 연구가 필요할 것으로 판단된다.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.4
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• pp.402-410
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• 2004

In this paper, an optimal design method of clonal selection based Fuzzy-Neural Networks (FNN) model for complex and nonlinear systems is presented. The FNNs use the simplified inference as fuzzy inference method and Error Back Propagation Algorithm as learning rule. Also Advanced Clonal Selection (ACS) is proposed to find the parameters such as parameters of membership functions, learning rates and momentum coefficients. The proposed method is based on an Immune Algorithm (IA) using biological Immune System and The performance is improved by control of differentiation rate. Through that procedure, the antibodies are producted variously and the parameter of FNN are optimized by selecting method of antibody with the best affinity against antigens such as object function and limitation condition. To evaluate the performance of the proposed method, we use the time series data for gas furnace and traffic route choice process.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.2
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• pp.239-245
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• 2012

This paper proposes a novel optimum design method for the PID controller of magnetic levitation-based Rail-Guided Vehicle(RGV) by a genetic algorithm using clone selection method and a new performance index function with performances of both time and frequency domain. Generally, since an attraction type levitation system is intrinsically unstable and requires a delicate controller that is designed considering overshoot and settling time, it is difficult to completely satisfy the desired performance through the methods designed by conventional performance indexes. In the paper, the conventional performance indexes are analyzed and then a new performance index for Maglev-based RGV is proposed. Also, an advanced genetic algorithm which is designed using clonal selection algorithm for performance improvement is proposed. To verify the proposed algorithm and the performance index, we compare the proposed method with a simple genetic algorithm and particle swarm optimization. The simulation results show that the proposed method is more effective than conventional optimization methods.

• Korean Journal of Construction Engineering and Management
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• v.14 no.3
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• pp.115-122
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• 2013

Construction Standard Productivity Manual (CSPM) has been used for estimating the contract amount of public construction projects. CSPM has been annually either revised and/or newly enacted with high costs. Therefore, This study makes use of CYCLONE, which is a simulation modeling and analysis system specialized in construction operation, to improve the revising and enacting processes of CSPM. This paper presents the CSPM revision module that is an Add-in to Web-CYCLONE. The new module can be effectively used to incorporate the auxiliary tasks and the non-productive tasks into AL-Form operation model. The outputs obtained from the models using he CSPM revision module and from work sampling were compared to verify the usability of the add-in modules. This study verifies that the new module can effectively assist enacting and/or revising CSPM.

• Korean Journal of Breeding Science
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• v.41 no.2
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• pp.126-129
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• 2009

The new Rubus coreanus Miq. variety, 'Doo-Ryun', was selected from 227 clones of the clone bank. This breeding was progressed to select the superior variety for high productivity from 1998 to 2005. To evaluate the clones, characteristics of a fruiting lateral and a ripe fruit were investigated. 'Doo-Ryun' was finally selected through selection of three times and stable trial at four different regions. 'Doo-Ryun' has specific morphological characteristics that are fewer thorn in lateral and strong glossiness of leaf upper side than contrast clone. The flowing and fruiting characteristics of 'Doo-Ryun' shown that the number of flower per fruitng lateral, the number of fruit per fruiting lateral, fruit weight and yield per individual were 17.2, 16.9, 1.73g and 4.50kg, respectively. Also, their selection effects were evaluated as 103.6%, 107.6%, 140.6% and 155.7% compared to the mean of whole clones in the clone bank, respectively.