• The Korean Journal of Zoology
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• v.29 no.4
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• pp.283-293
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• 1986

For the preliminary step to make and characterize the monoclonal antibodies of human alpha-fetoprotein (AFP) was purified from 534g of human fetal tissues through the procedures of tissue extraction, DEAE-cellulose, concanavalin A-Sepharose, Cibacron Blue F3GA-agarose and immunoadsorbent affinity chromatography. The isolated AFP preparation showed a single band on polyacrylamide gel electrophoresis and a single precipitin are against rabbit anti-human cord serum and anti-human AFP on immunoelectrophoresis. Our AFP also displayed a single band on SDS-polyacrylamide gel electrophoresis. The recovery of AFP was 8.76mg total.

• Proceedings of the Korea Information Processing Society Conference
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• 2017.11a
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• pp.2-5
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• 2017

기존의 어셈블러는 시각적으로 불편하고 사용자 편의를 위한 기능을 제공하지 않으며 최신의 컴퓨터와의 호환성 문제가 있었다. 이러한 문제점들의 해결책으로 나온 SIC/XE 어셈블러 시뮬레이터 오픈 소스를 GitHub에서 클론하여 분석하고 테스트하였다. 본 논문에서는 오픈 소스 SIC/XE 어셈블러 시뮬레이터의 다양한 오류를 분석하고 이를 수정하였다. 또한 리터럴 테이블, 심볼 테이블, 목적코드 및 오류 메시지의 시각화를 통해 기존의 SIC/XE 어셈블러 시뮬레이터를 개선시켜 사용자 편의를 높인 학습용 SIC/XE 어셈블러 시뮬레이터를 구현하였다.

• YAKHAK HOEJI
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• v.34 no.1
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• pp.11-14
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• 1990

$NH_2-terminal$ amino acid sequence analysis of monoclonal antibody is very important to identify gene family and diversities of antigen-antibody recognition. When we used the PVDF (Polyvinylidene difluoride) membrane blotting method, we could easily analyze $NH_2-terminal$ sequence of monoclonal-antibody which specifically binds to phosphatidylinositol 4,5-biphosphate. PVDF membrane is an ideal solid-phase support for sequence analysis, especially when used with electroblotting method. This method is superior to continual method and will be applied to the sequence analysis of picomole quantities of proteins by gel electrophoresis.

• The Korean Journal of Zoology
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• v.35 no.1
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• pp.29-36
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• 1992

The present study describes the production of monoclonal antibodies against cultured chick myoblast to pursue critical proteins in muscle cell fusion. Among a panel of monoclonal antibodies, three, Mll-3H 13, Mll-3Hl8 and Mll-3H35 were inhibited movblast fusion. A single 101-kDa antigen reactive with monoclonal antibody Mll-3H35 was detected by radioimmu-noprecipitation or by immunoblotting. During the course of myogenesis, the level of the protein remarkably decreased as the cells there differentiated. These results suggest that the protein platys a direct role in the process of myoblast fusion mechanism.

• The Korean Journal of Zoology
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• v.36 no.4
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• pp.522-528
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• 1993

Monoclonal antibodies (MAbs) against human alpha-fetoprotein (AFPI was produced by hybridizing SP 210-Ag 14 mouse myeloma cells with spleen cells of Balb/c mice immunized with purified AFP. Two subclones (D-6 and I-6) were expanded as ascite tumors in svngenic mice, and from ascitic fluid immunoglbulins were Pruified. Each anibodv was identified to be homogeneous by several criteria, and the affinity constant of D-6 and I-6 MAb to AEP was calculated to be 4.2${\times}$10-8 and 6.4${\times}$10-8 M-1, respectively. With these MAbs sensitive and accurate enzyme linked immunosorbent assay method was established.

• YAKHAK HOEJI
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• v.36 no.2
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• pp.188-190
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• 1992

Two clones of monconal antibodies(Co-1 and Co-2) against BSA-benzoylecgonine(BSABE) were produced. Both monoclonal antibodies showed high binding affinity to BSA-BE. Observing from ELISA inhibition assay, Co-1 reacted only weakly with soluble benzoylecgonine, while Co-2 showed considerable reactivity with soluble benzoylecgonine.

• The Magazine for Energy Service Companies
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• s.21
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• pp.62-67
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• 2003

최근 많은 미국 주정부에서는 재생 전기를 지원할 클린에너지기금을 조성하였다. 이 기금을 현재까지 조성했던 15개 주정부에서는 재생에너지 투자에 대해 1998년에서 2012년까지 35억달러를 조성할 예정이다. 이와 같은 현상은 재생에너지를 위한 진취적인 주정부에게는 새로운 경향이지만, 이 기금들의 초기경험을 보고하고, 공유하려는 노력은 거의 없었다. 클린에너지그룹과 제휴하고 있는 Berkeley 연구실은 이러한 클린에너지 기금의 사용에 대해 보고할 사례 연구를 시작했다. 이 연구의 주요한 목적은 정보의 추가적인 원천을 강조하고, 연락처를 확인하기 위해 주정부의(그리고 몇몇의 국제적인 사례) 클린에너지기름의 혁신적인 프로그램과 관리적인 실제에 대해 간단히 보고한다. 우리의 바람은 이 사례 연구가 새로이 확립된 클린에너지기금의 관리자들에게 유용하고, 신규로 클론에너지기금의 조성하려는 선봉적인 재생에너지 노력에 대한 학습에 관심을 가진 기금관리인에게 도움이 될 것이다라는 것이다. 새로운 몇 개의 사례 연구가 매년 추진될 것이다. 이번에 소개하는 사례는 매사추세츠 주정부의 그린빌딩프로그램으로 그린학교와 그린빌딩을 지원하기 위한 것이다(미 에너지부의 선도적인 클린에너지 기금 연구 보고서 중에서 발췌)

• Particle and aerosol research
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• v.10 no.4
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• pp.155-162
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• 2014

The internal temperature will change depending on operation conditions and material of cyclone dust collector. This study compares the results of collection efficiency and temperature distribution on the different heat flux at wall of dust collector. The previous researcher's experiment results were used to confirm the reliability of CFD(Computational Fluid Dynamics) model. Based on this verified CFD model, we extended the analysis on the cyclone dust collectors. In CFD study, we used RNG k-epsilon model for analysis of turbulence flow, fluid is air, the velocity at inlet is 10 m/s, the temperature of air is $600^{\circ}C$. Because of the difference of outer vortex and inner vortex temperature, the collection efficiency will reduce with the increase of heat flux, showed the highest collection efficiency at heat insulation.

• Journal of Plant Biology
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• v.36 no.1
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• pp.105-112
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• 1993

Selection of glyphosate-resistant clones from MNNG-treated mesophyll protoplasts of haploid tobacco and their differentiation were studied. The protoplasts were treated with 0.1 to 100 $\mu\textrm{g}$/mL N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for 30 min when they expanded to oval shapes. After the treatment, the protoplasts in 4-16 cell stages were transferred to the selective medium containing 1 mM glyphosate for the selection of the glyphosate-resistant colonies. The efficiency of the cell division of the protoplasts in the selective medium decreased as the MNNG concentrations in creased. Optimal MNNG concentration for induction of the glyphosate-resistant clones was 10$\mu\textrm{g}$/mL and mutation frequency was 2.66$\times$10-6. The stability of the glypohsate-resistance of the clones was examined by prolonged subculture in the medium with 1 mM glyphosate, and the resistant clones were survived more than 10 months. Among them one clone has been proliferating and greening and the others were proliferating without greening or greening with slower proliferating.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.318-323
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• 1988

MAK18 gene of Saccharomyces cerevisiae, needed for M1 replication, was mapped within 2cM of PET3 on chromosome VIII. From 38kb clone pRE66 carrying SPO11 and PET3, we have localized MAK18 gene whose insert is 2.8kb. MAK18 gene is Iocalized on about 9kb distance from PET3 and about 18kb distance from SPO11 on chromosome VIII.