• Resources Recycling
• /
• v.10 no.3
• /
• pp.14-22
• /
• 2001

For the recycle of coal fly ash generated from power stations, we developed an electrocyclone system which can separate unburned carbon form coal fly ash, based on the fact that coarse fly ash particles contain higher amount of unburned carbon and unburned carbon particles are charged positively, and pure ash particles are charged negatively on contacting each other. Additionally, guide vanes were installed in the cyclone to control the cut size. Two types of electrode, stick and grid type, were designed to investigate the effect of electrode type. Results show that by introducing an electric field inside the cyclone, the yield increases by 5 to 15e1o. But the content of unburned carbon in the clean ash does not change significantly.

• Biomedical Science Letters
• /
• v.1 no.1
• /
• pp.1-8
• /
• 1995

The present study was undertaken to determine the dissociation constant (Kd)of monoclonal antibody to human apolipoprotein A-I (apo A-I) using enzyme-linked immunosorbent assay (ELISA). First the monoclonal antibody was incubated in solution with the antigen until the equilibrium was reached; then the free antibody which remains unsaturated at equilibrium was captured by binding to antigen on the microtiter plate and be measured by a classical indirect ELISA. The value of Kd determined from Scatchard plot was 0.625$\times$10^{-9}$ for purified antibody and 0.720$\times$10$^{-9}$ for unpurified antibody. This method was valuable for the measurement of true dissociation constant and found to be simple, reproducible, and accurate.

• Korean Journal of Microbiology
• /
• v.40 no.2
• /
• pp.65-72
• /
• 2004

Instability of some eukaryotic sequence propagated in prokaryotic hosts is a frequently observed phenomenon. It is well documented that long inverted repeats, AT-rich sequences with structures like Z-DNA are extremely unstable in E. coli. These sequences may either be under-represented or even lost when cloned in E. coli. When we analyzed the polymorphic pattern for several tandom repeat (TR) in human SCKI gene, we found some TR regions were frequently deleted from plasmids and had difficult problem for their sequencing. These regions may result in non-clonability of the DNA sequence. Here we have cloned two difficult TR regions under low temperature and made two library for DNA sequencing using a nebulizer or sonicator. This study will help to determine the unstable genomic elements in complex mammalian genome.

• Clean Technology
• /
• v.20 no.3
• /
• pp.263-268
• /
• 2014

Using the CFD method, the pressure drop in 6 cyclone dust collectors of different shape were calculated. And the results were compared with results of the conventional theories. Equations of Shepherd and Lapple (1939, 1940), First (1950), Alexander (1949), Stairmand (1949), Barth (1956) were used in the theoretical calculation. In CFD calculations, we used standard k-epsilon model for analysis of turbulent flow, fluid is $25^{\circ}C$ air, the velocity at inlet is 10 m/s and the temperature is $25^{\circ}C$. In CFD analysis results, the pressure distributions along the flow showed similar patterns in different cyclone shapes. But the pressure drop distributions estimated on the conventional theories had big difference in different cyclone shapes. Only First's theory and CFD analysis showed similar results.

• The Korean Journal of Mycology
• /
• v.28 no.1
• /
• pp.26-31
• /
• 2000

We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.

• Korean Journal of Microbiology
• /
• v.51 no.2
• /
• pp.148-155
• /
• 2015

The diversity of endobacteria in the edible part (cap and stipe) king oyster mushroom (Pleurotus eryngii) was investigated using 16S rRNA sequence analysis. The bacterial 16S rRNA libraries were constructed from the body cap (BC) and the body stipe (BS) of the king oyster mushroom. The twenty sequenced BC clones were divided into four groups and the largest group was affiliated with the Firmicutes (40% of clones). While, the twenty sequenced BS clones could be divided into six groups and the largest group was affiliated with the Actinobacteria (40% of clones). The predominant bacterial family from both the cap and stipe of the mushroom was corresponded with the Gram positive bacteria (62.5%).

• Microbiology and Biotechnology Letters
• /
• v.16 no.5
• /
• pp.363-368
• /
• 1988

Molecular cloning of gene for $\alpha$-acylamino-$\beta$-lactam acylhydrolase (ALAH) III from Acetobacter turbidans has been attempted by immunochemical detection method, in which polyclonal antibody from mouse Balb/c against this enzyme was employed as a probe. As a cloning vector, λ gtll was chosen for this purpose. Two positive clones has been selected from genomic libraries of A. turbidans, which had somewhat different binding affinities on anti-ALAH III umm and anti-$\beta$-galactosidase. By restriction analysis, both clones has been turned out to lose one of EeoRI sites. From these results, it concluded that deletion of DNA between lacZ gene and inserted DNA has occurred during replication of these clones in host cells.

• Applied Biological Chemistry
• /
• v.49 no.3
• /
• pp.196-201
• /
• 2006

Vitellogenin, which is found in the serum of female and male fishes exposed to environmental endocrine disrupter or estrogen hormone, is used as a biomarker for environmental contamination with an endocrine disrupter. In order to produce antibody against vitellogenin, a synthetic peptide for partial vitellogenin was injected into rabbits. In addition, by using ion exchange chromatography on DE-52, vitellogenin was purified from the serum of carp induced with $17{\beta}$-estradiol. Polyclonal antibody against purified vitellogenin reacted well with vitellogenin in the serum of carp induced with $17{\beta}$-estradiol and the serum of female carp, whereas polyclonal antibody against the vitellogenin peptide did not react with proteins in those samples. This may indicate that vitellogenin proteins, covalently modified largely, could not be detected by Western blotting with the polyclonal antibody against the synthetic vitellogenin peptide.

• Korean Chemical Engineering Research
• /
• v.60 no.2
• /
• pp.289-294
• /
• 2022

Oil contained in domestic and industrial wastewater or marine spilled oil gives rise to severe environmental pollution issues such as water pollution and ecosystem destruction. The membrane filtration method as one of representative oil/water separation strategies has technological challenges such as membrane fouling and low separation rate. In this work, we devise a 3D-printed microhydrocyclone for oil/water separation by utilizing a digital lighting processing-based 3D printer. We demonstrate that the 3D-printed microhydrocyclone can effectively separate oil and water phases from oil-in-water emulsion.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.18 no.1
• /
• pp.93-100
• /
• 2016

This research was conducted to determine a pre-germinated herbicides with iron-coated seeds in water and wet hill seeded rice. Days of rice seedling emergence was the faster germination at the application of benzobicyclon > oxadiazon > thiobencarb > butachlor and the sealed iron-coated seeds with pre-germinated seeds> pre-germinated seeds> iron-coated seeds with pre-soaking. The seedling establishment was relatively high in untreated control and benzobicyclon among seed treatments by 63.7% and 75.7%. There was 100% seeds killed of pre-germinated seeds in terms of herbicide phytotoxicity and sealed iron-coated seeds with pre-germinated seeds in butachlor but benzobicyclon was of safe with 2% rice seeds killed. Infant rice seedling height was of 9.2-12.9cm in benzobicyclon and 11.9-16.3cm in untreated control and thus there was relatively normal development and growth at the initial rice seedling.