• Korean Journal of Microbiology
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• v.34 no.4
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• pp.194-199
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• 1998

Bacterial diversity was determined by amplification and sequencing of 16S rDNA at Tancheon and Jungrang in Han river. Twenty-seven clones constructed were divided 7 groups using RFLP. Fifteen clones were classified 4 groups in Tancheon and the group (HT-1 clone) including many clones was affiliated a high similarity with Aerobacter cryaerophilus (the class Proteobacteria including members of the delta subdivisions). The other two groups (HT-6 and HT-9 clone) including several clones were classified with the class Cytophagales in Tancheon. Twelve clones were classified 3 groups in Jungrang and the group (HJ-1 clone) including many clones was affiliated a high similarity with Sphingomonas sp. (the class Proteobacteria including members of the alpha subdivisions). As a whole results, the class Proteobacteria (alpha, beta and delta subdivision), the order Cytophagales, and the order Actinomycetales were detected.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.149-156
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• 1997

We have employed comparative RFL,P(Restriction Fragment Ixngth Pol~iniorphism) analysis and molecular phylogenetic techniques to investigate the diversity of uncultured microorganisms associated with the anaerobic PCP degradation in PCP-adapted enrichment cultures inoculated by samples from anaerobic cewage sludgc(Jangrim, Pusan) and leachate of landfill site(Kimhae). 16s rDNA cloncs were obtairted by PCR amplification of mixed population DNAs extracted directly from the nonactive and active stage ol each PCP-adapted culture. After three rounds of comparative RFLP analyses. two RFLP types. designated as Ala and Hld, were found prevalent and common in both active stage samples. Thc analysis of phylogenctic diversity bawd on the 5'-terminal 180 nt of sequences from whole clones of the Ala and Bld RFLP types showed close similarity among themselves. In case of Bld clones, 7XQ of them shared identical sequences. Thcse resuliq suggest that the clones of both RFLP types wcre originated from highly affiliated microorganisms which are e~iriched as a result of metabolic activity to PCP. The full-length 16s rRNA sequence of each representative clone from both RFLP types was determined. and an Ala clone w i n found to he related to Clo.strrdiurn ulfutzac~(Genk~ank No. Z69203) and a Bld clone to Thermobacteroides proteolyticus(Genbank No. X09335), with sequence similarities of 89%' and 97%. respectively.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2002.11a
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• pp.83-84
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• 2002

사이클론 집진기(이하 "사이클론"으로 표기)는 1800년대 후반에 기본개념이 싹트기 시작한 이후 100년 이상의 역사를 지닌 집진 장치로서, 구성이 단순하고, 고온 및 고압에서도 동작이 가능하며, 에너지 소모가 적고, 제조비용과 유지비용이 저렴한 특징을 지니고 있다. 현재 산업체에 설치되어 있는 싸이클론은 대용량의 가스처리, 입경 10-200$\mu\textrm{m}$까지의 분진처리, 낮은 초기설치비, 유지보수 및 조작의 간편성 때문에 산업체 여러 분야에서의 응용성은 다양하고 응용범위도 상당히 넓다고 할 수 있으며 석탄가스화 복합발전 플랜트, 쓰레기 소각로, 순환유동층 보일러 등에 널리 사용되고 있다. (중략)

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.485-492
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• 2013

The effect on collection efficiency with 2-stage vortex finder diameter and back mixing of solid flow due to swirling intensity, turbulence eddies, wall bouncing using the residence time distribution of particle flow in a common and modified cyclone. Higher collection efficiencies of fly ash in a modified cyclone(S = 13, 15cm) are showed than common cyclone. Collection efficiency in modified cyclone was highest at 2-stage vortex finder diameter, S = 13 cm. Variances of residence time distribution and average residence times of glass bead were increased with a diameter(S) of 2-stage vortex finder due to swirling intensity and turbulence eddies. Back mixing of solid flow in a modified cyclone were increased with a gas inlet velocity and showed higher than a common cyclone.

• Clean Technology
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• v.20 no.3
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• pp.241-250
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• 2014

In this study, performance and environmental characteristics of cyclone vacuum cleaners are analyzed and product improvement methods are investigated to minimize environmental effect of the cleaners using the result. A simplified LCA method is used to analyze environmental characteristics of the cyclone vacuum cleaners. Two cyclone vacuum cleaners with similar specifications are chosen for the experiment. Typical characteristics of cyclone vacuum cleaners such as energy consumption, suction force, noise and temperature are measured and compared. Most environmental effect was caused by the energy consumption in use phase of life cycle. Some ideas are created to reduce energy consumption of the vacuum cleaners in use phase like installing baffle, and methods to extend exchange period of filter. It is analyzed how recyclability rate of vacuum cleaners could be improved to reduce the environmental effect in whole life of the vacuum cleaners.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.263-268
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• 2008

Estimates of genetic gain (in volume growth) and diversity (expressed as status number, $N_s$) were determined in a clonal seed orchard of Pinus koraiensis. The genetic thinning was based on clonal breeding values (represented by general combining ability) obtained from progeny tests, clonal fertility estimated by strobilus production, and clonal size variation determined by the ramet numbers per clone. Parental GCA values for volume growth were calculated, based on height and diameter at breast height measured from field trials. Clonal fertility was estimated from the assessments of strobilus production over twelve years from 1991 to 2003, and used for the calculation of status number. There are 179 clones and 5,268 ramets in 12ha area of P. koraiensis clonal seed orchard. Genetic gain and diversity estimates were determined under assumptions of 30% pollen contamination and inferior genetic value of contaminating pollen. Genetic gain increased as thinning rates were set from 10% to 60%. However, for the higher thinning intensities, the increase of genetic gain was not remarkable. Genetic thinning by means of truncation selection resulted in a greater genetic gain but a large decrease in status number. Status number was represented around 40 clones for 10% through 60% thinning intensities, but for the higher thinning intensities, it was a bit fluctuated. Based on the present results, it could be concluded that thinning rate should not be stronger than 60% to optimize genetic gain while conserving genetic diversity. Consequently 50% or 60% thinning rate might be appropriate for genetic thinning in the clonal seed orchard of P. koraiensis. The effect of pollen contamination on the genetic gain and the consequence of genetic thinning for seed production in the clonal seed orchard, and seed orchard management scheme were also discussed.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.299-310
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• 1994

Intraspecific clonal interactions have important influences on a population structure of the cellular slime mold (CSM). This study was to investigate whether or not evolutionary change in a population could be induced by clonal competition, and to elucidate how various clones in a population evolve in a homogeneous environment of laboratory culture. The characteristic clones of Polysphondylium pallidum which had different resource consumption rates (RCR) and mating types I and II were selected for study. Investigation was conducted for 4 experimental time interval $(T_0-T_4)$; one experimental time interval took almost 10-14 days from inoculation to havest of fruiting bodies. Two sets of 50 clones were cultured from 50 clones at To, and RCR variations of the population were compared between $(T_0\;and\;T_4)$ for each set of clones. Each clone of the CSM had a diverse resource consumption rate, or growth rate, in a homogeneous and limited Cerophyl agar plate despite the passage of 48-56 generations from the beginning of the experiment. Diverse clones with different growth rate could coexist in one site of the homogeneous agar plate as well as heterogeneous soil microenvironment. When there was high clonal diversity of RCR, a clone in a population had high chances to encounter other clones with resultant increased clonal competition. In one set, 26 of 37 clones of mating type I were changed to mating type Il for the 4 experimental time intervals, which indicated that the rate of competitive exclusion among clones during total experiment from $(T_0\;to\;T_4)$ was 0.703. In another set, 31 of 37 clones of mating type I were changed to mating type II , having the rate of competitive exclusion 0.838. The frequency of each of mat~ng types changed by 0.93-1.29% in each successive generation. The competitive exclusion among clones occurred by 1.26-1.75% when approximately $2.6{\times}10^8$ bacterial cells were provided as food and thereafter one generation of myxamoebae of CSM elapsed at room temperature. This finding implicated that in the vegetative state of P, pallidurn there was 1.26-1.75% probabil~ty of evolutionary change per generation changing from one clone to another clone.

• Microbiology and Biotechnology Letters
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• v.26 no.6
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• pp.497-506
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• 1998

The expression in Escherichia coli of a cloned insecticidal protein (ICP) gene from Bacillus thuringiensis var. kurstaki HD1 in pHLN1-80 (+) and pHLN2-80(-) plasmids was investigated through deletions in promoters, transcription start point, and termination region. Six recombinant plasmids were constructed in an attempt to analyze the overexpression of the ICP in relations to its gene structure. The amounts of ICP produced from the recombinants were measured by SDS-PAGE and confirmed by Western blot analysis. One clone was not overexpressed which having only -80 bp (contained BtI promoter) part of the ICP gene promoter (without Plac promoter), the right-oriented ICP gene and the termination region. Removal of 350 bp from upstream region of the Plac of the clone pHLN2-80 (-) resulted in overexpression of the ICP. One clone was not overexpressed in which the clone consisted of -72 bp part of the ICP promoter without the transcription start point and the transcriptional termination region, and having the right-oriented ICP gene sequence. One clone consisting of the inverted ICP gene sequence, the -72 bp ICP gene promoter, and without the termination region caused overexpression. One clone which consisted of the inverted ICP gene, the -72 bp ICP gene promoter and the termination sequence was overexpressed. These results indicated that the Plac promoter, transcription termination region, the inverted ICP gene insertion, and the -80 bp or -72 bp part of the ICP gene promoters were concerned in the overexpression of the ICP gene in the recombinant plasmid, and also the overexpression mechanism might result from the disruption of the transcription-suppressing regions in the promoter regions.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2000.11a
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• pp.423-424
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• 2000

상대적으로 낮은 미세입자(주로 10$\mu$m이하) 제어효율은 기-고 싸이클론의 최대 약점으로 논의되어 왔다. 이전 연구에서 싸이클론의 유출가스에 포함되어 있는 미세분진 입자들을 2차적으로 분리하고 제거할 수 있는 Post Cyclone(PoC)이라는 장치를 개발하여 그 효용성을 입증하였고(Hoffmann,1996), Mita 등(Mita el. al., 1997)이 이론적 뒷받침을 위한 연구를 진행하여 이론적 모델을 제시하였다. 즉, PoC의 효율은 Reynolds 수와 Stokes 수의 함수로 표시될 수 있을 것으로 추정하였다. (중략)

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.6-12
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• 1990

The preS2 wequence of an adr hepatitis B virus was cloned and expressed in Escherichia coli as a $\beta$-galactosidase fusion polypeptide. Recombinant preS2 product interacted with the preS2-specific monoclonal antibody H8 which was induced by surface antigen particles isolated from a Korean gepatitis patient. The H8 showed only a minor cross-reactivity with recombinant preS2 product of adw2 subtype. Determination of nucleotide sequence of the adr preS2 revealed that twelve amino acid residue substitutions between adr and adw2 subtype sequences. The antigenic determinant to H8 must include some of these differences.