• Title/Summary/Keyword: 크로마토그래피

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Gas Chromatographic Determination of Flavor Stability of Cooking Oils (가스크로마토그래피에 의한 식용유의 향미 안정성 측정)

  • Kim, In-Hwan;Yoon, Suk-Hoo
    • Korean Journal of Food Science and Technology
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    • v.20 no.5
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    • pp.732-735
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    • 1988
  • Flavor stability of cooking oils such as rice bran oil, double fractionated palm olefin and soybean oil were determined by headspace analysis using gas chromatography. In the headspace, the contents of volatile compounds, oxygen and hydrogen were measured. The hydrogen content in the headspace correlated well with the contents of volatile compound (r > 0.95). Therefore, it is proposed that a single measurement of hydrogen and oxygen is used as a index of flavor stability of cooking oils instead of separate measurement of volatile compounds and oxygen. which have conventionally been used.

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Analysis of n-Hexanal in Headspace Vapor over Cooked Brown Rice by Direct Vapor Injection Gas Chromatography (현미취반시 생성되는 n-hexanal의 가스크로마토그래피에 의한 분리및 정량)

  • Shin, Myung-Gon;Rhee, Joon-Shick;Kwon, Tai-Wan
    • Korean Journal of Food Science and Technology
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    • v.17 no.5
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    • pp.406-408
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    • 1985
  • n-Hexanal in headspace over the cooked brown rice stored at $5^{\circ}C$ and $35^{\circ}C$ for 0. 4, 8 and 12 months was determined by a modified direct vapor injection gas chromatographic method. The retention time of n-hexanal was 3.5 min and n-hexanal could be rapidly separated from other compounds at the operational conditions of gas chromatography. n-Hexanal contents of cooked brown rice also showed a standard deviation of less than 10% of the average.

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Reversed-Phase Ion-Pair Chromatography of Aromatic Sulfonic Acids (방향족 슬폰산들의 역상 이온쌍 크로마토그래피)

  • Sam-Woo Kang;Hae-Beom Oh;Seung-Seok Lee
    • Journal of the Korean Chemical Society
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    • v.30 no.2
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    • pp.224-230
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    • 1986
  • Dodecyltrimethylammonium bromide (DTAB) was examined as a counter-ion in the separation of aromatic sulfonic acids on alkylmodified silica (ODS) column by reversed-phase ion-pair chromatography. The capacity factor of acids was investigated and separation was attempted. The capacity factor of samples was influenced by the several factors such as a concentration of counter-ion, concentration of methanol, kinds and concentration of electrolyte in mobile phase as well as kinds and position of functional group in samples. Some mixtures of samples were able to be separated under optimum condition.

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Chromatographic Separation of Lithum Isotopes by Hydrous Managanese(Ⅳ) Oxide (가수된 산화 망간(Ⅳ)에 의한 리튬 동위원소의 크로마토그래피적 분리)

  • Kim, Dong Won
    • Journal of the Korean Chemical Society
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    • v.45 no.3
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    • pp.219-222
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    • 2001
  • Separation of lithium isotopes was investigated by chemical ion exchange with a hydrous manganese(IV) oxide ion exchanger using an elution chromatography. The capacity of manganese(IV) oxide ion exchanger was 0.5 meq/g. The heavier lithium isotope was enriched in the solution phase, while the lighter isotope was enriched in the ion exchanger phase. The separation factor was determined according to the method of Glueckauf from the elution curve and isotopic assays. The separation factor of $^6Li^+$-$^7Li^+$ isotope pair fractionation was 1.018.

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Determination of Low Molecular Weight Organic Acids in Pacific Silver Fir Leachates by Using Ion Chromatography (Ion chromatography에 의(依)한 나뭇잎의 저분자량(低分子量) 유기산(有機酸) 분석(分析))

  • Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.21 no.3
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    • pp.22-29
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    • 1993
  • 나뭇잎으로부터 발생되는 저분자량 유기산을 이온크로마토그래피를 이용하여 분석하였다. 이 방법은 자연상태의 나뭇잎 (needle litter)을 유기용매의 추출 없이 그대로 이용할 수 있어서 사용하기에 편리하다. 본 논문에서는 이온 크로마토그래피의 사용방법과 침엽수잎(Pacific Silver Fir)의 부후정도에 따른 유기산의 종류와 농도의 변이를 간략하게 소개한다. 초기의 침엽수잎 여과액으로부터는 $C_2$에서 $C_4$까지의 아세트산(acetic acid), 프로피온산(propionic acid), 옥살산(oxalic acid) 그리고 포름산(formic acid) 등이 주종을 이루지만 부후정도가 커짐에 따라 방향핵을 가진 방향핵 유기산(aromatic organic acid)이 발생한다.

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Development of Immunochromatography for the Rapid Detection of Listeria monocytogenes (Listeria monocytogenes 신속 검출을 위한 면역크로마토그래피법의 개발)

  • Choi, Jin-Gil;Shim, Won-Bo;Je, Jung-Hyun;Kim, Ji-Young;Lee, Kyu-Ho;Kim, Min-Gon;Ha, Sang-Do;Kim, Keun-Sung;Kim, Kwang-Yup;Kim, Cheol-Ho;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.299-303
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    • 2007
  • The objective of this study was the development of immunochromatography (ICG) for the rapid and accurate detection of Listeria monocytogenes. Here, monoclonal antibodies (MAb) were conjugated with 40 nm colloidal gold particles, where the conjugate was used as the detection reagent in the ICG. The ICG was composed of three pads (sample, conjugate, and absorbance pads) and one nitrocellulose membrane. The colloidal gold-MAb conjugate was applied to the conjugate pad, and the test line and control line on the membrane were treated with MAb (FKLM-3BI2-37) and anti-mouse IgG, respectively. The detection limit of the ICG was $10^{5}$ cell/mL and it showed no cross-reaction to food borne pathogens. We inoculated meat and lettuce samples with various counts of L. monocytogenes, and analyzed them by ICG. All the inoculated meat samples gave positive results after enrichment for 24 h in LEB. These results indicate that ICG was able to serve as a primary screening tool for L. monocytogenes in various foods and agricultural products within 20 min after enrichment.