• 한국임상수의학회지
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• 제31권3호
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• pp.216-219
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• 2014

1세, 암컷, 17 kg의 잡종견이 갑작스러운 허탈증상으로 본원에 내원하였다. 혈액 검사 결과에서 심각한 저칼슘혈증을 보였으며, 방사선 사진 영상에서는 장 폐색을 의심하게 하는 가스로 가득 찬 장확장증 소견을 나타내었다. 칼슘 글루코네이트의 정맥 내 투여를 시작한 후 4시간째에 장 확장 소견은 경감되었다. 혈청 부갑상샘호르몬 농도는 저칼슘혈증에 비해 정상 범위 아래로 측정되었으며, 이를 기초로 하여 환견은 일차성 부갑상샘기능저하증으로 진단되었다. 칼슘 글로코네이트와 칼시트리올의 투여 3일 후, 환견의 임상증상과 전해질 불균형은 개선되었다. 본 보에서는 일차성 부갑상샘기능저하증으로 진단된 개에서 일반적으로 관찰되지 않는 증상인 심각한 위장관 확장증의 발생 예를 보고한다.

• Journal of Pharmaceutical Investigation
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• 제39권4호
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• pp.309-314
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• 2009

An enzyme immunoassay (EIA) was validated for quantitation of cacitriol in human plasma. Calcitriol was immunoextracted with immunocapsules, which contain monoclonal antibodies to calcitriol linked to solid phase particles in suspension with a vitamin D binding protein inhibitor. Calcitrol was eluated and the eluates were evaporated under a gentle stream of nitrogen gas. The absorbance of analytes was determined using a microplate reader (reference wavelength 650 nm; measurement wavelength 450 nm). The method was specific and sensitive enough to detect as low as 6.5 pmol/L of calcitriol. Linear calibration range was 6.5-491 pmol/L with correlation coefficient greater than 0.99. The overall accuracy was in the range of 83.8 to 111.2% and precision C.V. (%) 0.99 to 8.47%. The recovery was approximately 100% and stability was confirmed during storage and sample preparation. The pharmacokinetic parameters were calculated by baseline subtraction because calcitriol is an endogenous material. Following oral dose of calcitriol, the mean AUC$_{24h}$ was 1038${\pm}$539 pmol/Lhr and C$_{max}$ of 128${\pm}$63.1 pmol/L was reached at 3.50${\pm}$1.07 hr. The mean t$_{1/2}$ of calcitriol was 5.13${\pm}$2.10 hr. The present EIA method was successfully applied to study bioavailability after oral administration of 2 ${\mu}$g of calcitriol in healthy Korean subjects.