• Journal of Periodontal and Implant Science
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• v.27 no.4
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• pp.941-946
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• 1997

• Journal of Periodontal and Implant Science
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• v.13 no.1
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• pp.61.1-61.1
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• 1983

• The Journal of the Korean dental association
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• v.43 no.12 s.439
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• pp.799-808
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• 2005

이 연구의 목적은 치수 및 치근단 조직의 감염의 주요 원인들이 Porphyromonas endodontalis의 LPS가 치주인대 섬유아세포의 MCP-1 MIP-1α 그리고 RANTES의 생성에 미치는 영향을 관찰함으로써 치근단 염중의 발병에서 섬유아세포의 역할을 알아보는 것이다. 혐기성 조건에서 배양한 P. endodontalis와 E. coil의 LPS를 추출, 정제한 후, 치주인대 섬유아세포 0.01, 0.1, 1, 10ug/ml 농도의 P. endodontalis와 E. coil의 LPS로 12, 24, 48시간 동안 자극하였다. MCP-1, MIP-1α 그리고 RANTES의 단백질 농도는 ELISA를 이용하여 분석하였다. 이연구의 결과는 아래와 같다. 1. P. endodontalis의 LPS로 자극시킨 치주인대 섬유아세포에서 분비된 MCP-1의 수준은 시간과 농도에 비례하여 증가하였다. 2.E.coil의 LPS로 자극시킨 치주인대 섬유아세포에서 분비된 MCP-1의 양도 시간에 비례하여 증가함을 보였다. 이는 P. endodontalis LPS보다 통계적으로 유의성있게 많은 양을 나타냈다.(p<0.05) 3. 치주인대 섬유아세포에서 MIP-1α 과 RANTES는 거의 분비되지 않았다. 이 연구에서 세균의 LPS로 자극시 치주인대 섬유아세포 MCP-1의 강한 발현은 만성 치근단 염증의 초기에 단핵세포와 대식세포의 recruitment를 증가시키는 것고 관련되어지는 것으로 보인다.

• Journal of Periodontal and Implant Science
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• v.15 no.1
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• pp.89-89
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• 1985

• The Journal of the Korean dental association
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• v.47 no.5
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• pp.252-259
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• 2009

치면세균막관리는 치주병과 치아우식증을 예방하는 데 필수적이다. 이러한 치면세균막관리는 자가관리와 전문가관리로 구분할 수 있는데, 이 두가지를 병행하였을 때 특히 건강한 치주를 유지하는데 도움을 준다. 자가관리는 1회성 교육으로는 불충분하며 체계적인 반복교육이 필요하다. 전문가관리는 대상자의 구강상태에 따라 일정한 주기로 계속 시행되어야 하며 $3{\sim}6$개월 주기가 바람직하다. 반복교육과 계속관리과정으로 진행되는 자가 및 전문가 치면세균막 관리 중에 대상자의 구강병 위험요소에 따라 불소도포와 치면열구 전색 등이 제공될 수 있다.

• Journal of Dental Rehabilitation and Applied Science
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• v.35 no.3
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• pp.160-169
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• 2019

Purpose: The purpose of this study was to evaluate the antimicrobial effects of a toothbrush with light-emitting diodes (LEDs) on periodontitis-associated dental biofilm attached to a zirconia surface by static and dynamic methods. Materials and Methods: Zirconia disks (12 mm diameter, 2.5 mm thickness) were inserted into a 24-well plate (static method) or inside a Center for Disease Control and Prevention (CDC) biofilm reactor (dynamic method) to form dental biofilms using Streptococcus gordonii and Fusobacterium nucleatum. The disks with biofilm were subdivided into five treatment groups-control, commercial photodynamic therapy (PDT), toothbrush alone (B), brush with LED (BL), and brush with LED+erythrosine (BLE). After treatment, the disks were agitated to detach the bacteria, and the resulting solutions were spread directly on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy (SEM) was performed to visualize alterations in bacterial morphology. Results: No significant difference in biofilm formation was observed between dynamic and static methods. A significant difference was observed in the number of viable bacteria between the control and all experimental groups (P < 0.05). The percentage of bacterial reduction in the BLE group was significantly higher than in the other treated groups (P < 0.05). SEM revealed damaged bacterial cell walls in the PDT, BL, and BLE groups, but intact cell walls in the control and B groups. Conclusion: The findings suggest that an LED toothbrush with erythrosine is more effective than other treatments in reducing the viability of periodontitis-associated bacteria attached to zirconia in vitro.

• Proceedings of the Korea Contents Association Conference
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• 2017.05a
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• pp.409-410
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• 2017

본 연구는 편백 에션셜 오일을 이용하여 치아우식증과 치주질환을 예방하기 위한 효과적인 구강양치액을 연구개발하고자 수행되었다. Streptococcus mutans에 대한 항미생물 효과 및 치면세균막 형성에 미치는 영향을 실험실연구(in vitro)와 전임상연구(preclinical observations)를 통해 조사하였다. 편백에센셜오일은 실험실 연구결과 S. mutans 성장 억제효과와 항미생물 효과를 보였고, 전임상시험 결과 자원자들의 치면세균막 지수가 감소하였다. 편백 에센셜 오일은 뛰어난 항미생물 효과와 치면세균막 억제를 보이는 것으로 나타났다.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.4
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• pp.586-590
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• 2009

Leukocyte adhesion deficiency is rare inherited defect on phagocytic function resulting lack of leukocyte cell surface expression of $\beta2$ integrin molecule that are essential for leukocyte adhesion to endothelial cells and chemotaxis. Clinical features of patients with leukocyte adhesion deficiency type I include recurrent necrotic infection of the skin mucous membranes, and intestinal tract with septicemia, and omphalitis arising from delayed umbilical cord separation. Oral manifestations are severe progressive periodontitis with alveolar bone loss, periodontal pockets, and partial and total premature loss of the deciduous and permanent dentitions. We report a case of leukocyte adhesion deficiency type I in a 5-year-old child with severe periodontitis. In order to prevent local and systemic infection, we controlled periodontal disease with periodic oral prophylaxis. Oral swabs and blood cultures were perfomed for suspected infection, so that optimal measures were taken through the use of appropriate antibiotics.

• Journal of Korean society of Dental Hygiene
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• v.20 no.5
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• pp.743-752
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• 2020

Objectives: The objectives of this study were to investigate the distribution and level of periodontopathic bacteria with the general characteristics and oral health condition of the elderly. Methods: A total of 335 elderly individuals aged 65 years or older who lived in Ganghwa-gun, Incheon, were included in the study. Oral examination, investigation through a questionnaire, and collection of saliva were carried out. The collected saliva was analyzed for the distribution and levels of bacteria (red and orange complex bacteria) by real-time polymerase chain reaction. Statistical analyses were performed using chi-square test, t-test, one-way analysis of variance, and Pearson's correlation coefficient with SAS statistical software version 9.4. Results: Among the general characteristics, there were significant differences in the distribution of Porphyromonas gingivalis, Treponema denticola, and Parvimonas micra depending on sex, age, and dental visits (p<0.05). The number of remaining teeth and denture use were related to the distribution of periodontopathic bacteria, except T. denticola (p<0.05). Additionally, periodontitis was related to the distribution of P. gingivalis (p<0.05). As the number of remaining teeth increased, the copy number of red and orange complex bacteria also increased (p<0.05). Those individuals who did not use dentures and had periodontal disease had more periodontopathic bacteria (p<0.05). Conclusions: The distribution and copy number of periodontopathic bacteria in the elderly were more related to oral health condition than to general characteristics. In particular, the distribution and copy number of periodontopathic bacteria were higher in subjects with multiple remaining teeth, no dentures, and periodontal disease.

• Journal of Periodontal and Implant Science
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• v.15 no.1
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• pp.87.2-87.2
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• 1985