• Journal of Periodontal and Implant Science
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• v.28 no.1
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• pp.161-175
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• 1998

치주상태의 진단은 일반적으로 치주탐침과 같은 임상적 검사와 보조적으로 방사선 검사를 이용한다. 방사선학적인 검사가 치주조직의 파괴정도와 골 흡수를 진단하는데 유용하지만 실제적인 골 흡수를 정확하게 나타나는데는 한계가 있다고 하였다. 그리고 임상적 측정과 방사선 사진을 비교연구하여 이들간의 관계를 알아보고자 하는 노력이 있었다. 방사선이 임상에서 참고사항으로는 중요하지만 절대적 신뢰를 주는데는 문제가 있는 것으로 생각되어왔다. 방사선학적 측정과 임상지수 측정간의 상관관계에대하여 계속적인 연구를 통해 규명하는 것이 중요하다고 생각되어 저자는 연세대학교 치과 대학병원 치주과를 내원한환자중 연령 및 성별에 관계없이 전신질환이 없는 환자 130명을 대상으로 소구치의 원.근심부, 대구치의 원.중.근심부에서 치주낭의 깊이와 (P.D), 부착상실 (A.L)을 측정하였다. 방사선 사진상의 원.근심부에서 백악 법랑질 경계부에서 치조골능 상부까지의 치조골 수준 (Rx.B.L)을 측정하였고 이개부 부위에서는 이개부의 천정으로부터 나타나는 골 흡수의 방사선 투과상을 측정하였다. 이를 기초로 임상지수와 방사선학적 측정간에 상관관계를 연구하여 다음과 같은 결론을 얻었다. 1. 원·근심부의 P.D와 Rx.B.L을 비교 했을 때 상관관계는 모든 치아에서 높게 나타났다(r=0.897$\sim$r=0.725, p<0.01). 2. 원·근심부의 A.L와 Rx.B.L을 비교 했을 때 상관관계는 모든 치아에서 높게 나타났다 (r=0.915$\sim$r=0.732, p<0.01 ). 3. 대구치 769개가 실험 대상인었다. 상악 대구치중(356개) 40%가 이개부 병소를 나타냈고 (146개), 하악 대구치중(413개) 41%가 이개부 병소를 나타냈다(170개). 4. 대구치의 이개부 병소의 중앙부에서 측정한 P.D와 Rx.B.L을 비교 하였을 때 상관관계는 상악 제1대구치를 제외한 모든 치아에서 높게 나타 났다(r=0.811$\sim$r=0.435, p<0.01). 5. 대구치의 이개부 병소의 중앙부에서 측정한 A.L와 Rx.B.L을 비교 하였을 때 상관관계는 상악 제1대구치를 제외한 모든 치아에서 높게 나타 났다(r=0.906$\sim$r=0.479, p<0.01). 이상의 결과를 종합하여 볼 때 임상지수와 방사선학적 측정을 비교한 바, 이들의 상관관계는 모든 치아에서 밀접하게 나타났다. 따라서 방사선학적 측정은 임상지수와 더불어 치주질환의 진단과 치료계획에 크게 기여할 수 있을 것으로 사료된다.

• Journal of Dental Rehabilitation and Applied Science
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• v.28 no.1
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• pp.15-25
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• 2012

The relationship between occlusion and periodontal health has been extensively studied. However, reports on the effects of passive eruption using occlusal reduction has not been sufficient. The purpose of the present randomized clinical trial was to assess the influence of passive eruption using occlusal reduction on the clinical periodontal parameters consisting of attachment level, pocket depth, tooth mobility, width of keratinized gingiva and osseous defect. The study was performed on 40 teeth of 16 subjects who have been treated for the moderate periodontitis at the Department of Periodontology, Pusan National University Hospital. At the baseline examination, after hygienic-phase and after 6 month from passive eruption using occlusal reduction, clinical parameters were monitored and radiographs were taken. The 20 teeth in the test group received passive eruption using occlusal reduction while the 20 control teeth did not receive any occlusal reduction. The results were as follows; 1) Degree of inflammation of periodontium was improved by initial therapy 2) Teeth received passive eruption using occlusal reduction demonstrated significantly greater reduction in pocket depth, tooth mobility and amount of bone loss, and increase in the width of keratinized gingiva, but no significant changes in the attachment level compared to the control teeth 3) There was significantly greater reduction in pocket depth, mobility, amount of bone loss and attachment level in the test teeth after initial hygienic phase when compared with baseline data. Taken together, these results suggest that the passive eruption using occlusal reduction would be helpful to improve periodontal health.

• Journal of Periodontal and Implant Science
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• v.35 no.2
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• pp.271-275
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• 2005

본 연구는 치주낭에 biofilm형태로 부착되어 질환을 유발시키고 항생제 빚 항균제에 저항을 일으키는 세균 독성요소를 규명하기 위해 시행된 기초연구이다. 치주질환의 주 병원균의 하나인 Porphyromonas gingivalis 381 biofilm의 세포외막에 특이하게 발현되는 단백질을 규명하기 위한 기초적인 자료를 얻기 위해 시행하였다. Porphyromonas gingivalis 381을 통상적인 세균 배양용 broth를 사용하여 혐기성 세균 배양기로 24시간 배양한 것을 대조군으로 하고, tissue culture plate를 이용하여 혐기성 배양조건 하에서24시간동안 biofilm을 형성하여 실험군으로 설정하였다. 세균을 수획하여 세포외막을 분리하고 isotonic isoelectric focusing을 시행한 결과 주로 약 20-30 kilodaltons에 해당하는 수종의 세균세포막 단백질이biofilm으로 배양한 세균에서 더 상승적으로 발현됨이 관찰되었고, 상이한 수종의 단백질도 planktonic culture broth로 배양한 세균에서 다 상승적으로 발현됨을 관찰할 수 있었다. 이것은 세균의 배양조건과 환경에 따라 그 외막 단백질이 서로 다르게 발현됨을 입증하는 기초적인 자료로서 향후 단백질의 동정과 성격을 규명하는 근간 실험으로 추진할 계획이다.

• Journal of Dental Rehabilitation and Applied Science
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• v.37 no.1
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• pp.31-38
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• 2021

Purpose: The purpose of this study was to investigate effect of heme on periodontopathogens. Materials and Methods: The experiment was performed using 7 types of anaerobic bacteria present in the periodontal pocket. The bacteria were cultured using suitable medium in an anaerobic condition with or without hemin, and the growth of the bacteria was measured every 6 hours by a spectrophotometer. Results: the growth of Porphyromonas gingivalis was different only by the presence or absence of hemin. The growth of other periodontopathogens except Treponema denticola was different in a hemin concentration-dependent manner. The growth of T. denticola was interfered by hemin. Conclusion: Heme may be a factor that leads dysbiosis in the microbial ecosystem of the subgingival plaque and thereby promote a periodontitis-causing environment.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.3
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• pp.448-452
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• 2004

Trauma from occlusion(TFO) is injury to the periodontal tissue as a result of occlusal forces. Mobility is a common clinical sign of occlusal trauma. In acute occlusal trauma, this may be accomanied by pain, tenderness to percussion, thermal sensitivity, and pathologic tooth migration. Chronic occlusal trauma may be marked by excessive wear and gingival recession. Radiographic finding include a widened periodontal ligament space, radiolucence and condensation of the alveolar bone and root resorption. TFO is related to the pathogenesis of periodontal disease. It can cause increased tooth mobility TFO itself does not initate or aggravate marginal gingivitis or initiate periodontal pockets. Active trauma can accelerate bone loss, pocket formation and gingival recession depending on the presence of local irritants and inflammation. Gingival recession associated with occlusal forces includes traumatic crescent, McCall's festoon and Stillman's cleft. TFO plays a minor role in the pathogenesis of early to moderate periodontitis. A 5-year-old male visited Yonsei University Pedodontics clinic with a chief complaint about gingival recession. Mobility, excessive wear, gingival recession were detected by clinical exam on the both mandibular deciduous ca nine. On the radiographic view, vertical alveolar bone loss was observed on both mandibular deciduous canine.

• Journal of Periodontal and Implant Science
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• v.26 no.4
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• pp.779-797
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• 1996

The present study investigated the effects of variations in decalcification time of demineralized freeze-dried bone on the osteogenic potential of DFDB. Sixteen 3-wall intrabony defects with 4mm depth were surgically created in the mesial aspect of upper and lower anterior teeth of 4 dogs. Following the flap procedure, three test groups with 4 defects each received either freeze-dried bone graft (Group I), demineralized freeze-dried bone graft decalcified for 12hours (Group II), or demineralized freeze-dried bone graft decalcified for 24hours(Group III). The rest of the four defects received the flap procedure-only as the control group. The healing was histologically analyzed after 14 weeks on the length of connective tissue adhesion, new bone formation and new cementum formation. The results were as follows: 1. The length of connective tissue adhesion showed no statistically significant difference in all groups with $0.62{\pm}0.14mm$ for Control, $0.42{\pm}0.11mm$ for Group I, $0.63{\pm}0.43mm$ for Group II and $0.52{\pm}0.11mm$ for Group III. 2. The new bone formation showed no statistically significant difference in all groups with $3.17{\pm}0.24mm$ for Control. $3.15{\pm}0.56mm$ for Group I. $3.22{\pm}0.36mm$ for Group II, and $3.28{\pm}0.74mm$ for Group III. 3. The new cementum formation showed no statistically significant difference in all groups with $4.19{\pm}0.46mm$ for Control, $3.23{\pm}0.64mm$ for Group I, $4.13{\pm}1.82mm$ for Group II. and $3.13{\pm}0.62mm$ for Group III.

• Journal of dental hygiene science
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• v.16 no.6
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• pp.472-480
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• 2016

In this study, 110 adults aged 40 to 69 years were surveyed from April 28, 2016, to May 28, 2016 to analyze their periodontal status according to daily stress, coping methods, and oral health behavior. The collected data were analyzed using the t-test, one-way analysis of variance, and hierarchical multiple regression. Daily stress levels of all subjects were most frequent potential risk 64.5% of the subjects, the high risk 19.1% and 16.4% of the health group. Regarding stress coping methods, active methods recorded 2.46, passive methods recorded 2.32. Regarding oral health behaviors, subjects brushed an average of 2.45 times daily, for an average of 2 minutes. Futhermore, 69.1% of subjects brushed before bedtime and 89.1% practiced scaling. Regarding periodontal status, the O'Leary index was 73.45, gingivitis index was 1.30, an average of 2.83 quadrants possessed a periodontal pocket. The hierarchical multiple regression analysis identified, type of employment (${\beta}=-0.348$), scaling (${\beta}=-0.253$), and age (${\beta}=0.244$) as factors that influence the number of quadrants possessing a periodontal pocket. These results confirmed that the oral health behavior of scaling, but not stress levels of coping methods, strongly influenced periodontal status.

• Journal of dental hygiene science
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• v.12 no.5
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• pp.469-476
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• 2012

The purpose of this study to grasp the effects of the stress coping method on the periodontal disease in order to provide raw data for the studies on the development of the training for effective stress copying method resulting in improvement in prevention, occurrence, and progression of periodontal disease. Data were collected from total of 326 subjects in their forties and fifties, during the period from March to October 2011. Using questionnaire and examination of periodontal health, and were analyzed using frequency, t-test, multivariate analysis of variance, multiple regression by SPSS ver. 19.0 (IBM Co., USA). The higher emotion-focused stress coping ($\beta$=0.341), the higher the gingival index, the higher the emotion-focused stress coping ($\beta$=0.239) the deeper the pocket depth, the higher the emotion-focused stress coping ($\beta$=0.158) the higher clinical attachment loss, showed statistically significant positive association. This study suggests that the dental clinic institute needs to implement education program including stress management and coping method improvement in order to prevent periodontal disease and make the occurrence and progression to be managed by professionals.

• Journal of Periodontal and Implant Science
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• v.24 no.3
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• pp.607-617
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• 1994

To determine the effect of tenascin on forming periodontal pocket and pseudopocket, the ginival tissues were surgically obtained from the patients with adult periodontitis(10) and non-inflammatory phenytoin-associated gingival hyperplasia(5). The excised tissue specimens were fixed in neutral formalin for $6{\sim}24$ hours, embedded with paraffin, sectioned at 4-6m in thickness, mounted on glass slides coated with 3-aminopropyltriethoxysilane(Sigma Chemical Co., St. Louis, MO, U.SA.) and immunohistochemically processed by Avidin-Biotin peroxidase complex method for the localization of tenascin, using monoclonal mouse anti-human tenascin antiboday(Chemicon-International Inc., Temecula, CA, U.S.A., 1: 5,000) as the primary antibody. Regardless of periodontal pocket and pseudopocket, tenascin was localized along the connective tissue subjacent to basement membrane of gingival epithelium, and strong positive reactivity was obviously noted in the papillary projections of gingival connective tissue. The results suggest that tenascin may affect the development of papillary projections and the proliferation of epithelial cells.

• Journal of Periodontal and Implant Science
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• v.31 no.4
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• pp.777-785
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• 2001

치주질환이 진행되는 동안이나 치주처치후 치유되는 과정에서 치조골의 변화가 야기되는데 방사선 사진은 치조골 변화를 감지하는 유일한 비외과적인 방법이다. 미묘한 치조골 변화의 진단은 치료시나 유지관리기 환자의 평가시 중요한 바, 최근에는 규격화시킨 디지털 영상을 이용하여 정량적인 골변화 측정이 가능하게 되었다. 본 연구의 목적은 중등도의 치주질환을 지닌 환자에서 국소마취하에 초기 치주처치를 시행한후 참조체와 함께 구내 방사선 사진을 촬영하고 디지털화 한 다음 참조체 당량치를 이용하여 치조골의 밀도변화를 평가하기 위한 것이다. 이 연구를 위하여 치주질환에 이환된 환자 5명(남자 3명, 여자 2명 : 평균 47.4세)에서 탐침깊이가 5㎜ 이상이고 골내낭이 있는 제 $1{\cdot}2$ 소구치, 제 $1{\cdot}2$ 대구치 40개(상악 24개, 하악 16개)를 대상으로 구강위생교육과 치석제거술, 치근면활택술을 시행하였다. 임상지수는 술전과 술후 8주째에 측정하였고, 방사선 사진은 술전, 술후 2주, 4주, 6주, 8주째에 촬영하였고, 구리 스텝웨지를 사용하여 규격화 하였다. 촬영된 영상은 NIH image program(U.S.A)에 의해 분석되어졌고 이들 자료를 통해 다음과 같은 결과를 얻었다. 1. 치조골의 밀도는 초기치료후 2주째 까지는 감소된 양상을 보이다가 4주 이후로는 점차적으로 증가하는 양상을 보였다. 2. 치조골의 밀도는 초기치료전과 비교시 초기치료수 6주째와 8주째에서 유의한 차이를 보였다.(p<0.01) 3. 상하악 간의 치조골 밀도는 유의한 차이를 보이지 않았다(p>0.05). 이상과 같은 결과를 통하여 볼때 초기 치주처치만으로도 치조골의 밀도가 증가됨을 확인할 수 있었다.