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Studios on the Metagonimus fluke in the Daecheong Reservoir and the upper stream of Geum River, Borea (대청호 및 그 상류의 Metagonimus 흡충에 관한 연구)

  • 김종환;김남만
    • Parasites, Hosts and Diseases
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    • v.25 no.1
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    • pp.69-82
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    • 1987
  • The prevalences of the cuke belonging to genus Metagonimus hove been reported along the upper stream of inhabitants by several workers since 1980, however the taxonomical problems of the fluke was not yet settled. The larval flukes; cercaria and metacercaria as well as their intermediate hosts, and adult were studied in order to identify the Mepagonimus in the areas. The results obtained are summarized as follows: 1. The snails, Semisulcospira globus were collected (rom the three different localities along the upper stream of the River. The cercariae were found from 125(7.2%) out of 1,730 snails by natural emerging method, and were identified into 5 species including Metagenimus sp. (3.7%), Pseudexorchis major(1.4%), Cercaria nipponensis (0.9%), Cercaria incerpa(0.6%), and Cercaria yoshidae(0.6%). Cercariae of Metagonimus species had four to dye oral spines on its anterior of the first line. 2. The cercariae of Metagonimus were experimentally exposed to goldfish. nfection rate was 22.9% out of 105 goldfish, and the encysted metacercariae were found in fins(86.7%) and on scales (13.7%) of the fishes, but not in their muscle, head or visceral organs. 3. Seven species of ask were caught in the Daecheong Reservoir and the upper stream. Infestations with metacercaria of Metagonimus were found 100% in Opsariichtys widens and the parasitized numbers of the metacercariae were observed from 250 to 2,400 per fish. In the upper stream, Zacco termmincki, Z. platypus and Pseudogobio esocinus were infected 100% with the metacercaria, on the other hand, the fishes caught in the reservoir showed the lower infestation rates, and a few metacercariae found in the fishes Carassius carassius and Cyprinus carpio in the reservoir and the stream. The majority of metacercariae was detected only on the scales of fishes. 4. In order to know the infectivity and the distribution patterns in the intestine of hosts, rats and dogs were infected with the metacercariae obtained from O. bidens and Z. platypus. In addition the metacercariae obtained from Z. temmincki, P. esocinus and goldfish were given to the rats. The recovery rates of the worms in the small intestine of dogs were higher (63.3~65.8%) than those of the rats (3.5~31.6%). The flukes were found mostly in the middle and the lower part of small intestine of the rats and the dogs, but no worm was collected in the upper part of the intestine of rats. 5. The sixte of adult flukes varied by the hosts. In the adult cukes, oral sucker was smaller than ventral sucker, and the right and left testes were located diagonally, the uterine tubules circled around the upper left testis. The average egg sixte was $29.1{\times}1.7{\mu\textrm{m}}$. According to the above results, the cukes belonging to genus Metagonimus distributed along the Geum River was concluded to be identical with Miyata type of M. yokogawai as that Saito had proposed.

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Histopathological and Serological Observations on Experimental Anisakiasis of Rabbits (실험적 토끼 아니사키스증에서의 조직학적 병변과 혈청 항체가의 경시적 변동 양상)

  • 홍성태;이손형
    • Parasites, Hosts and Diseases
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    • v.25 no.2
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    • pp.168-180
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    • 1987
  • This study was performed to observe histopathological changes and serological reactions in chronic anisakiasis of rabbits. Each rabbit was infected per os with 30 larvae of Anisakis type I. Their sera were collected chronologically and the rabbits were killed for histopathological examination, 3, 13, 20, 30, 60, 90 and 150 days after the infection. The results were summarized as below. 1. Most of the larvae were recovered from the stomach, but a few from the omentum, intestine, mesentery and abdominal wall. The recovery rates and distribution of worms by organ were not differed by duration of infection. 2. Histologically the lesion was abscess type on 13 days, i.e., the dead worms were surrounded by fibrinous exudate, histiocytes and thick zone of numerous inflammatory cells. After 30 days, histiocytes were found to invade the worms and the lesion was changing into abscessgranulomatous type. Also a calcified worm was found on the 30th day. After then the worms were observed to be dissolved slowly until 90 days. On 150 day, only one calcified worm was observed. 3. The levels of serum IgG antibody by ELISA reached their maximum 30 days after the infection. After then, it decreased slowly until 150 days after the infection. Above serological and histopathological findings indicated that antigenic stimulation from degenerating Anisakis larvae was the greatest during the first 30 days after infection. This period was corresponding with the beginning of worm resolution or calcification. Serologic test by ELISA would be a valuable tool for confirming chronic anisakiasis.

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Detection of Toxoplasma antigens and antibodies in mice infected with different strains of Toxoplasma gonnii (톡소포자충의 충주에 따른 항원과 항체의 검출 시기 및 양상)

  • 이영하;김재영
    • Parasites, Hosts and Diseases
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    • v.33 no.3
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    • pp.201-210
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    • 1995
  • This study aims to assess the possible strain-dependent variations in detection of ToxopLosmn antigens and antibodies. The virulent RH strain or avirulent Beverley strain of T gondii were injected into mice, intraperitoneally, and their antigens, antibodies and parasites were identified from the blood or tissues: liver, brain and spleen by ELISA, Western blot and PCR. In mice infected with RH strain, circulating antigens and parasitemia were first detected from 2 days after infection, and ToxopIasma DNA were found in the blood, liver, brain and spleen from 3 days after infection. It was impossible to detect specific IgM and IgG antibodies to T gondij and any specific band was not found by Western blot. In mice infected with Beverley strain, circulating antigens were detected between day 10 and day 35. The Toxoplusma DNA was found in the blood and liver from day 15 until day 60, and in the brain from day 20. But Toxoplosma DNA in the spleen were mainly detected between day 10 and day 30. The IgM antibodies were first appeared on day 10 post-infection, and were noted obviously increased between day 15 and 25. The IgG antibodies were first detected on day 15, and showed progressively increased titers. The antibody binding bands were specific according to infection period. Sera from mice infected with Beverley strain reacted mainly with the antigen of 27.5-kDa and 32.5-kDa. In conclusion, mice infected with RH strain revealed Toxoplosma antigens strongly, but not antibodies. However. mice infected with Beverley strain revealed both the Toxoplasma antigens and antibodies. The present results showed that immune responses are different between avirulent and virulent T gonnii.

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Analysis and Design of Profiling Adaptor for XML based Energy Storage System (XML 기반의 에너지 저장용 프로파일 어댑터 분석 및 설계)

  • Woo, Yongje;Park, Jaehong;Kang, Mingoo;Kwon, Kiwon
    • Journal of Internet Computing and Services
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    • v.16 no.5
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    • pp.29-38
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    • 2015
  • The Energy Storage System stores electricity for later use. This system can store electricity from legacy electric power systems or renewable energy systems into a battery device when demand is low. When there is high electricity demand, it uses the electricity previously stored and enables efficient energy usage and stable operation of the electric power system. It increases the energy usage efficiency, stabilizes the power supply system, and increases the utilization of renewable energy. The recent increase in the global interest for efficient energy consumption has increased the need for an energy storage system that can satisfy both the consumers' demand for stable power supply and the suppliers' demand for power demand normalization. In general, an energy storage system consists of a Power Conditioning System, a Battery Management System, a battery cell and peripheral devices. The specifications of the subsystems that form the energy storage system are manufacturer dependent. Since the core component interfaces are not standardized, there are difficulties in forming and operating the energy storage system. In this paper, the design of the profile structure for energy storage system and realization of private profiling system for energy storage system is presented. The profiling system accommodates diverse component settings that are manufacturer dependent and information needed for effective operation. The settings and operation information of various PCSs, BMSs, battery cells, and other peripheral device are analyzed to define profile specification and structure. A profile adapter software that can be applied to energy storage system is designed and implemented. The profiles for energy storage system generated by the profile authoring tool consist of a settings profile and operation profile. Setting profile consists of configuration information for energy device what composes energy saving system. To be more specific, setting profile has three parts of category as information for electric control module, sub system, and interface for communication between electric devices. Operation profile includes information in relation to the method in which controls Energy Storage system. The profiles are based on standard XML specification to accommodate future extensions. The profile system has been verified by applying it to an energy storage system and testing charge and discharge operations.

A Cases of Crane Breeding(養鶴) in the Palace of the Joseon Dynasty Period (조선시대 궁궐에서의 양학(養鶴) 사례)

  • Hong, Hyoung-Soon
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.3
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    • pp.1-10
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    • 2020
  • The purpose of this study is to identify whether the cranes had been bred in the palace of the Joseon Dynasty period and to consider the related cases. The temporal range of this study is in the Joseon Dynasty period, and the spatial range is throughout the entire palace, including the naejeon(內殿) and oijeon(外殿), and government offices inside(闕內各司) and government offices outside(闕外各司). The reference materials for this study were partly extracted and translated from the original documents to consider, and a Korean version of documents was used in the database of the Institute for the Translation of Korean Classics. The results of this study are summarized as follows. First, the cranes were bred from the early Joseon Dynasty Era in Uijeongbu, the highest government office in the Joseon Dynasty period. After the Japanese Invasion of Korea in 1592, crane breeding in Uijeongbu(議政府) seems to have been suspended due to the damage to the government building and the change in the status of the government office. Second, crane breeding in Hongmungwan(弘文館), which was responsible for the classics colloquium(經筵) and public opinions and assisted the king by the side, continued from the early Joseon Dynasty period(Jungjong's Era) to the late Joseon Dynasty period(Jeongjo's Era) after the Japanese Invasion of Korea in 1592. Third, in the Jeongjo's Era, the cranes were also bred in Gyujanggak(奎章閣), which was newly established as the central institution of learning to strengthen the royal authority. At that time, it seems that several cranes were bred in Gyujanggak. Fourth, it is judged that 'Crane breeding' in the core government offices of Joseon, such as Uijeongbu, Hongmungwan, and Gyujanggak, was meaningful as a symbol of identities, such as the status and character of the institution. Fifth, it seems that the cranes bred in the palace, including Hongmungwan, were conventionally brought by the Baecheon County of Hwanghae-do. This convention caused minor conflicts between the central and local government offices during the Yeongjo's Era, but it seems to have continued throughout the Jeongjo's Era. In this study, there is a limit that most of the studies were conducted based on local data. If further data discovery and translation outcomes are accumulated in the future, more abundant cases will be identified. The deepened follow-up studies are also needed, other than the cases of rearing cranes in the local government offices and temples.

Studies on Witches' Broom of Ligustrum ovalifolium Hasskarl Caused by Mycoplasma-like Organism (MLO) (Mycoplasma성(性) 왕쥐똥나무 빗자루병(病)에 관(關)한 연구(硏究))

  • Chai, Jyung-Ki;Kim, Young-Ho
    • Journal of Korean Society of Forest Science
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    • v.78 no.2
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    • pp.103-118
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    • 1989
  • The occurrence of witches' broom in Ligustrum ovalifolium was first noticed in Korea by author in 1984. The present study was carried out with particular emphasis on the symptomatology, etiology, transmission of the disease and antibiotic treatments. The infected tissue was observed by the fluorescence and electron microscopy and its biochemical characteristics were compared with healthy one by electrophoresis. The results are summarized as follows : 1. symptoms of the infected trees were characterized by the dwarfing of the organs, yellowing and brooming of the foliage. 2. The observation by the trans electron microscopy on the witches' broom of L. ovalifolium revealed the occurrence of numerous mycoplasma-like organisms(MLOs) in the phloem tissue cells of the midribs of infected leaves. 3. The MLOs were surrounded by a single unit membrane, and they appeared to be multiplied by binary fission. 4. The presence of crystals unidentified in the phloem parenchyma cells was noticed by electron rnicroscopy, 5. The disease was able to be transmitted by budding, crown, and greenwood graftings to L. ovalifolium, L. obtusifolium, L, japonicum and also transmitted, even when the stocks and scions were not completely grafted. 6. Insect transmission on L. ovalifolium and L, obtzrsifolium was carried by Hishimonus sellatus. 7. The infected roots dipped in the 1,000 ppm of teracyclin solution was only temporarily effective in controlling the disease. 8. Infected plant with MLOs showed specific fluorescent reactions in phloems with DAPI stain. 9. The protein and peroxidase separated by electrophoresis showed strikingly distinctive difference between the healthy and diseased leaves.

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황금배 동녹 방지용 및 갈색배 방균.방충처리용 봉지 개발

  • 류정용;여성국;신종호;송봉근;한점화
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2000.04a
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    • pp.148-149
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    • 2000
  • 황금배는 1967년에 신고에 이십세기를 교배하여 1977년 1차선발과 1982년 2차선발을 거 쳐 1984년 최종선발, 명명한 품종으로, 당도가 높고 육질이 부드러워서 최근 몇년 사이에 캐 나다, 미국, 호주, 그리고 유럽 지역에서의 수요가 급증하고 있는 수출전망이 매우 밝은 품 종 중의 하나이다(92년 재배면적 lOha 수출량 5. 8M/T, 95년 재배면적 150ha 수출량 2 200.6M/T), 황금배는 비교적 대과이고 과형은 원형에 가까운 편원형으로서 사과 골든처럼 과피가 황금색이고 과육은 연황백색으로 투명하며 보기에 극히 미려한 특징이 있다. 아울러 육질이 유연치밀하고 과즙이 극히 많으며 당도가 높아 13$^{\circ}$ Bx이상, 15도 Bx까지 측정되는 둥 감산이 적화되어 맛이 극히 우수하다. 그러나 이러한 황금배는 동녹, 흑반병 등 병충해로 인한 상품가치의 하락으로 현재 수요를 충족시키지 못하고 있는 실정이다. 1 16세기부터 씌워진 과실봉지는 초기 병해충을 방지할 목적만으로 사용되어 왔지만, 현 재는 방균과 방충의 효과와 함께 자연현상의 최적화를 위한 차광성, 발수성, 투기성을 조절 하며 과실의 외관까지 영향을 미치는 바, 과실봉지의 기능성 부여를 위해서는 고도의 기술 력이 요구되고 있다 하겠다. 상기한 배에 방균방충처리된 과설봉지를 씌워서 재배하면 농 약 살포횟수를 줄이고 배에 농약이 직접 묻지 않아 배의 농약오염을 예방할 수 있으며, 봉 지 안으로의 해충이나 균의 침투를 원천적으로 봉쇄할 수 있다. 그러나 기존의 황금배용 봉 지는 비록 기타 병충해 피해를 방지하는 효과는 있었으나, 동녹을 억제하는 효력이 다소 미 흡하였다. 과피의 비정상적인 코르크화로 인해 발생하는 동녹은 과피의 물리적 할렬과 생리적 장 해에 의해 발생하는 것으로 알려져 있다(永澤 1940). 과실이 비대해짐에 따라 과피의 기공 (과점)이 할렬하면서 코르크화가 진행되는데 그 발생정도나 시기는 배의 품종에 따라 다르 나 일반적으로 코르크화는 기상조건, 특히 습도와 밀접한 관련이 있다고 알려져 있다 황금 배의 재배에 봉지를 적용하면 일반적으로 과피의 코르크화가 억제되는데 그러한 이유는 다 음과 같이 설명할 수 있다. 과실은 하루를 주기로 하여 수축과 팽창을 반복하면서 비대화하 는데 이러한 현상은 과실 내의 수분 조건에 따르는 것으로, 봉지재배의 경우 무대재배보다 단기간에 변화되는 습도의 범위가 좁아 급변을 방지하기에 과점의 할렬이 완화될 수 있다. 즉, 봉지를 씌웅으로서 봉지 내의 대기 환경이 외기보다 안정적으로 유지되고 직사광선이나 농약 및 마찰로부터 과실을 보호해 주기에 동녹이 어느 정도 방지될 수 있는 것이다. 그러나 기존의 황금배봉지는 동녹의 정도를 완화시킬 뿐 완전히 방지할 수 없었으며, 봉지를 적 용한 재배조건에서의 동녹발생 기구를 정확히 이해하지 못했었기에 효과적으로 봉지의 기능 을 개선하는 것이 불가능하였다. 과설의 미려도는 과실의 맛과 함께 그 가치를 결정짓는 중요한 물성으로서 우리나라 황 금배 재배환경과 특성에 알맞은 배봉지의 제작이 선결될 때, 배 품질의 향상, 안정된 공급이 가능하게 될 것이며 아울러 농가의 수업증대와 수출 경쟁력 강화가 이루어질 수 있을 것으로 판단된다. 이러한 측면에서 황금배 재배농가가 당면한 동녹발생의 문제점을 신속한 해결 을 위한 새로운 기능성 국산 황금배 봉지의 개발이 절실히 요구되고 있다. 위와 같은 문제를 해결하기 위하여 본 연구에서는 과실봉지의 종류간에 동녹발생 정도 가 상이한 점에 예의 주시하여 다양한 봉지의 적용실험을 통해 다음과 같은 결과를 얻었다. 황금배의 동녹 발생 정도는 배봉지의 발수성과 투기 및 투습도에 의해 크게 영향받는다. 상기한 바와 같이 과점의 코르크화로 인해 동녹이 발생된다고 할 때, 봉지 내의 습기 및 웅결수의 양은 황금배의 동녹에 중대한 영향을 미친다. 태양광이 내려찍는 낮 시간동안 황 금배는 증산작용을 하며 습기를 배출하는데 봉지 내의 온도가 높은 낮 시간 동안 수분이 습기로 존재하지만 기온이 급격히 떨어지는 일몰 이후에는 상대습도가 높아짐에 따라 결로 현상으로 인해 응결수가 된다. 이때 응결수와 접촉한 과피는 건조한 상태보다 세균의 침입 이 용이할 뿐만 아니라 기공(과점)의 호홉에 지장이 초래됨에 따라 과점의 할렬이 더욱 조 장되어 코르크화를 유발하고 결과적으로 동녹이 발생한다고 판단된다. 따라서 만일에 봉지 의 투기, 투습도가 양호하여 봉지 내의 과다한 수분이 충분히 배출될 수 있었다면, 수분의 응결을 피하고 동녹을 완화시킬 수 있을 것이라 판단되었다.

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A New High Qualilty Rice Variety with High Head Rice Ratio and Milling Recovery, "Chilbo" (완전미율, 도정수율 높은 중만생 고품질 벼 신품종 "칠보(七寶)")

  • Kim, Jeong-Il;Chang, Jae-Ki;Park, No-Bong;Yeo, Un-Sang;Oh, Byeong-Geun;Kang, Jung-Hun;Kwon, Oh-Deog;Shin, Mun-Sik;Park, Dong-Soo;Kwak, Do-Yeon;Lee, Jong-Hee;Song, You-Cheon;Kim, Chun-Song;Cho, Jun-Hyun;Yi, Gihwan;Lee, Ji-Yoon;Nam, Min-Hee;Kim, Sang-Yeol;Ahn, Jong-Woong;Ku, Yeon-chung;Kim, Jae-Kyu
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.547-551
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    • 2009
  • A new commercial rice variety "Chilbo" is a japonica rice (Oryza sativa L.) with resistance to rice stripe virus and high grain quality. It was developed by the rice breeding team of Yeongdeog Substation, NICS, RDA in 2007. This variety was derived from a cross between "Yeongdeog 26" with wind tolerance and lodging resistance and "Koshihikari" with good grain quality. A promising line, YR21324-119-3-2 was selected by a pedigree breeding method and designated as "Yeongdeog 44" in 2004. Regional adaptation yield trials were carried out at eleven locations from 2005 to 2007. As a result, "Yeongdeog 44" was released as a high yielding rice variety with high grain qualilty and virus resistance with the name of "Chilbo". It is short 76cm in culm length and has medium-late growth duration. This variety is resistant to stripe virus and middle resistant to leaf blast disease. It is also tolerant to cold, dried wind. Milled rice kernel of "Chilbo" is translucent, clear in chalkiness. Panel test proved that and it has good eating quality. Head rice ratio of Chilbo is high compared to the check variety, Hwaseongbyeo. Yield potential of "Chilbo" in milled rice is about 5.57MT/ha at ordinary fertilizer level of local adaptability test. This variety would be adaptable to Yeongnam plain, south & east-south coastal, south mid-mountainous, middle plain area of Korean peninsula.

A New High Qualilty Rice Variety with Lodging Resistance and Multiple Resistance to Diseases, "Donghaejinmi" (중만생 고품질 내도복 복합내병성 벼 신품종 "동해진미(東海珍味)")

  • Yeo, Un-Sang;Kim, Jeong-Il;Lee, Jeom-Sig;Park, No-Bong;Chang, Jae-Ki;Oh, Byeong-Geun;Kang, Jung-Hun;Kwak, Do-Yeon;Cho, Jun-Hyun;Lee, Jong-Hee;Kwon, Oh-Deog;Lee, Ji-Yoon;Nam, Min-Hee;Kim, Sang-Yeol;Ku, Yeon-Chung;Kim, Jae-Kyu
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.288-291
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    • 2009
  • A new commercial rice variety "Donghaejinmi" is a japonica rice (Oryza sativa L.) with lodging resistance and high grain quality. It has been developed by the rice breeding team of Yeongdeog Substation, National Institute of Crop Science (NICS), RDA. This variety was derived from a cross between "Milyang 64" as a resistance source of brown planthopper (Bph) and "Milyang 165" as grain quality source. The donor parent, "Milyang64" has been backcrossed three times with recurrent parent, "Milyang165" and selected by the pedigree breeding method. The pedigree of "Donghaejinmi", designated as "Yeongdeog 41" in 2003, was YR21259-B-B-68-1. It has a short culm length with 69 cm and medium-late growth time. This variety is resistant to stripe virus and moderately resistant to leaf blast disease with durable resistance. It also has tolerance to unfavorable environment such as cold, dried wind and storm. Milled rice kernel of "Donghaejinmi" is translucent, clear in chalkness and good at eating quality in panel test. The merit of this variety is high head rice ratio, which is essential element to produce an article of superior quality rice brand. The yield potential of "Donhaejinmi" in milled rice is about 6.05 MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to Yeongnam inland plains and eastern costal area of Yeongnam province.

Detection and Assessment of Forest Cover Change in Gangwon Province, Inter-Korean, Based on Gaussian Probability Density Function (가우시안 확률밀도 함수기반 강원도 남·북한 지역의 산림면적 변화탐지 및 평가)

  • Lee, Sujong;Park, Eunbeen;Song, Cholho;Lim, Chul-Hee;Cha, Sungeun;Lee, Sle-gee;Lee, Woo-Kyun
    • Korean Journal of Remote Sensing
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    • v.35 no.5_1
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    • pp.649-663
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    • 2019
  • The 2018 United Nations Development Programme (UNDP) report announced that deforestation in North Korea is the most extreme situation and in terms of climate change, this deforestation is a global scale issue. To respond deforestation, various study and projects are conducted based on remote sensing, but access to public data in North Korea is limited, and objectivity is difficult to be guaranteed. In this study, the forest detection based on density estimation in statistic using Landsat imagery was conducted in Gangwon province which is the only administrative district divided into South and North. The forest spatial data of South Korea was used as data for the labeling of forest and Non-forest in the Normalized Difference Vegetation Index (NDVI), and a threshold (0.6658) for forest detection was set by Gaussian Probability Density Function (PDF) estimation by category. The results show that the forest area decreased until the 2000s in both Korea, but the area increased in 2010s. It is also confirmed that the reduction of forest area on the local scale is the same as the policy direction of urbanization and industrialization at that time. The Kappa value for validation was strong agreement (0.8) and moderate agreement (0.6), respectively. The detection based on the Gaussian PDF estimation is considered a method for complementing the statistical limitations of the existing detection method using satellite imagery. This study can be used as basic data for deforestation in North Korea and Based on the detection results, it is necessary to protect and restore forest resources.