• Title/Summary/Keyword: 충격 지속 시간

Search Result 78, Processing Time 0.025 seconds

Electroporation Conditions for DNA Transfer into Somatic Embryogenic Cells of Zoysia japonica (들잔디 체세포 배발생 세포로의 DNA 전입을 위한 Electroporation 조건 구명)

  • 박건환;안병준
    • Korean Journal of Plant Tissue Culture
    • /
    • v.25 no.1
    • /
    • pp.13-19
    • /
    • 1998
  • We have reported previously that intact embryogenic cells can be used instead of protoplasts for electroporation-mediated transformation of zoysiagrass and rice. In this study, conditions of the tissue electroporation were examined to optimize the procedures. Embryogenic cell suspensions were established in liquid MS medium containing 2 mg/L of 2,4-D with embryogenic calluses induced from mature embryos of Z. japonica. The suspension-cultured cell clumps were electroporated with 35S-gusA expression vector DNA, and degrees of DNA introduction into the cells were determined by histological expression rates of the gusA marker gene. DNA transfer into the cell clumps occurred in wide range of voltage (100-400 V) and capacitance (10-1980 $\mu\textrm{F}$), but more in the ranges of 200-300 V and 330-800 $\mu\textrm{F}$ DNA concentrations higher than 6 $\mu\textrm{g}$/mL were adequate for GUS expression of the electroporated cells. DNA transfers were confirmed in all three embryogenic cell lines but only in one out of eleven non-embryogenic lines. Positive GUS expressions occurred with DNAs added even 20-40 h after pulse treatments. As a promoter of gusA, Act1 and Ubi1 were effective 7 and 5 times than 35S respectively in number of GUS expression units on electroporated cell clumps. Embryogenic cell clumps survived and regenerated into plantlets after pulse treatments of wide range of conditions.

  • PDF

Damage of Overlaid Concrete Structures Subjected In Thermally Transient Condition by Rainfall (강우에 따른 콘크리트 덧씌우기 보수체의 손상에 관한 연구)

  • 윤우현
    • Journal of the Korea Concrete Institute
    • /
    • v.13 no.5
    • /
    • pp.491-498
    • /
    • 2001
  • The failure phenomenon of overlaid concrete structures, such as surface crack and peel-off failure in the contact zone, was investigated due to temperature shock(rainfall). To investigate this failure phenomenon, the surface tensile stress, and the shear stress, the vertical tensile stress in the contact zone were analysed using the non-linear stress-strain relationship of material such as strain-hardening- and strain-softening diagrams. Rainfall intensity, overlay thickness and overlay material were the main variables in the analyses. It is assumed that the initial temperature of overlaid concrete structures was heated up to 55$\^{C}$ by the solar heat. With a rain temperature 10$\^{C}$ and the rainfall intensity of nR=1/a, tR=10min, 60min, the stress states of overlaid concrete structures were calculated. The result shows that only fictitious cracks occurred in the overlay surface and no shear bond failure occurred in the contact zone. The vortical tensile stress increasing with overlay thickness was proved to be the cause of peel-off failure in the contact zone. The formulae for relationship between the vertical tensile stress and overlay thickness, material properties were derived. Using this formulae, it is possible to select proper material and overlay thickness to prevent failure in the contact zone due to temperature shock caused by rainfall.

A Study on the Characteristics of Dynamic Behaviors for Continuous PSC Girder Bridges with Integral Pier Cap (교각일체형 연속 PSC 거더교의 동적거동 특성 연구)

  • Jeong, Young Do;Koo, Min Se;Yi, Seong Tae;Kim, Hee Sung
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.17 no.1
    • /
    • pp.94-105
    • /
    • 2013
  • Recently, the construction industry has been changed in such a way that the cost for bridge construction should be optimized or reduced. Therefore, bridges are required be cost-effective in terms of initial construction as well as in the maintenance during service stage. In order to reduce the cost for bridge construction, the Rahmen typed structure, in which the bridge components from superstructure to substructure are integral, has many advantages to reduce the size of structural members including girders, since the loadings from superstructure may be transferred to substructure through the connecting rebars such as stud, etc. This paper studied on the continuous Up and Down Prestressed Concrete (UD PSC) girder bridge in which the reinforced concrete pier cap is integral with the part of girders in superstructure. In previous studies, it is known that the structural behavior of continuous UD PSC girder bridge is quite different compared to the one of the bridges with conventional bearings or shoes to support the loading from girders. Nevertheless, it has hardly been studied about the structural behavior of bridge with UD PSC girder. Therefore, in this study, various dynamic behaviors of continuous UD PSC girder bridge with integral pier cap have been analyzed using numerical method. Furthermore, an equation to evaluate the impact factor is suggested for the UD PSC girder bridge which has two to three continuous spans.

주요 외국주가와 우리나라 주가의 상관관계에 관한 연구

  • Yun, Pyeong-Gu
    • The Korean Journal of Financial Studies
    • /
    • v.6 no.1
    • /
    • pp.203-221
    • /
    • 2000
  • 본 연구는 선진국들의 주가변동이 우리나라 증시에 미치는 영향, 아시아 각국과 우리나라 주가변동의 상관관계, 우리나라와 이들 국가간 주가변동 상관관계의 시계열적 안정성, 우리나라와 이들 국가간 주가변동 전이의 방향 및 시차에 관하여 분석 하였다. 우리나라 증시 개방시점을 전후하여 1981년 1월$\sim$1991년 12월을 제1기간, 1992년 1월$\sim$1999년 8월을 제2기간으로 한 각국 주가지수의 월별수익률과 1999년 1월 4일$\sim$1999년 8월 24일 기간중 각국 주가지수의 일별수익률을 자료로 상관분석과 회귀분석을 실시한 결과는 다음과 같다. 첫째, 우리나라 증시에 영향을 미친 선진국으로는 제1기간중에는 영국, 일본, 미국의 순이며, 제2기간중에는 전적으로 일본과의 상관관계가 높게 나타났다. 특히 일본과의 상관관계는 제1기간보다도 제2기간중에 더욱 상승하였다. 또한 선진국들 간에는 독일, 영국, 프랑스 3국간 주가변동의 상관관계가 높게 나타났다. 둘째, 아시아 각국과 우리나라의 상관관계분석에서는 태국, 인도네시아, 말레이시아와는 상관관계가 높게 나타났으나, 대만, 싱가폴과는 상관관계가 비교적 낮게 나타났다. 그리고 아시아 각국간에는 홍콩, 말레이시아, 태국, 인도네시아 국가들의 상호간 상관관계가 높게 나타났다. 셋째, 상관관계의 시계열 안정성에 관한 분석에서는 1995년 이전까지는 전체적으로 우리나라와 각국간 주가변동의 상관관계가 낮거나 국가별로 매우 불안정한 상태를 나타내고 있으나, 1996년 이후부터는 상관관계가 점점 높아지는 추세에서 1998년도에만 우리나라의 IMF충격으로 인하여 일부국가들과의 상관관계가 일시적으로 낮아진 상태를 보이고 있다. 개별국가별로는 우리나라와 일본과의 상관관계가 지속적으로 높아지고 있으며 태국, 인도네시아와는 IMF사태이후 크게 높아진 것으로 나타났다. 넷째, 주가변동방향 및 시차분석에서는 미국, 영국, 독일의 주가변동이 1일의 시차를 두고 우리나라에 영향을 미치고 있으며, 일본을 비롯한 동남아 각국과 우리나라간에는 주가변동에 시차가 없는 것으로 나타났다. 그러나 각국간 표준시차 및 거래소 거래시간을 고려하면 미국, 영국, 독일의 경우에도 그 시차는 1일이내이거나 거의 시차가 없는 것으로 판단된다.

  • PDF

Weathering Phenomena of the Rocks Constituting the Seoggulam (석굴암을 이루는 암석의 풍화현상)

  • Lee, Sang Hun
    • Journal of Conservation Science
    • /
    • v.8 no.1 s.11
    • /
    • pp.1-9
    • /
    • 1999
  • The present aspect of the Seoggulam is the result of natural weathering of the rocks and influences by mankind. The rocks constituting the Seoggulam is granodiorite of medium and coarse grains which show brown to dark brown weathered surface resulted mainly from chemical weathering of feldspars. It also shows partly milky white or black colors due to influences by weathering products of cement used in the concrete dome and cracks. There are cracks and crack zones with very small cracks which may be formed by strong weathering and influences of shocks during operations of reconstitution and repair. However, for the better conservation, it must be considered to maintain the balances of temperature and moisture, especially relative moisture in places within the room, structural stability, and the influences of vibration made up by some reasons.

  • PDF

A Study on the Effect of the Stemming Hole medium to the Blasting Separation Distance of Structure (공내 매질이 구조물의 발파이격거리에 미치는 영향에 관한 연구)

  • Kang, Hee-Seop;Jeong, Jung-Gyu;Bang, Myung-Seok
    • Journal of the Korea institute for structural maintenance and inspection
    • /
    • v.21 no.1
    • /
    • pp.100-108
    • /
    • 2017
  • Because of urbanization, Industrialization and expansion of transportation network, blasting works are recently increasing in construction field. The blasting work influences environmental effects to residents and the safety of facilities around the working place, so the development of blasting technology is needed to reduce the damage to residents. The blasting mechanism in the hole was studied and tested in the blasting sites by the difference of diameter between explosives and drilling hole, which is named by the decoupling effect. This effect was tested by changing the medium between explosives and hole wall in three working sites(railway, highway and industrial complex). The vibration velocity of blasting was recorded and vibration equations were produced by regression analyses. Finally, the structure separation distance was derived using these equations. The testing results show that the specific gravity of medium is larger, the separation distance is smaller and the duration time of blasting is shorter in case of large specific gravity of medium, so the vibration effect stops more fastly in the water compared with the air.

Comovement and Forecast of won/dollar, yuan/dollar, yen/dollar: Application of Fractional Cointegration approach and Causal Analysis of Frequency Domain (한·중·일 환율 사이의 움직임 분석 - 분수공적분과 진동수영역의 인과성 -)

  • Jung, Sukwan;Won, DooHwan
    • International Area Studies Review
    • /
    • v.21 no.2
    • /
    • pp.3-20
    • /
    • 2017
  • Traditional co-integration analysis method is known to be difficult to clearly determine the relationship between the cointegrated variables. This study utilizes a fractional cointegation method and a causal analysis of time and frequency domain among the exchange rates of Korea, China and Japan. The results show that even though traditional cointegration methods did not clarify the existence of cointegration, exchange rates were fractionally cointegrated. Causal analysis of time domain and frequency domain provided somewhat different results, but the yen/dollar was useful for forecasting won/dollar and yuan/dollar. Proper use of causal analysis of frequency domain and fractional cointegration emthods may provide useful information that can not be explained from the traditional method.

Development of Modification Coefficient for Nonlinear Single Degree of Freedom System Considering Plasticity Range for Structures Subjected to Blast Loads (폭발 하중을 받는 구조물의 소성 범위를 고려한 비선형 단자유도 시스템의 수정계수 개발)

  • Tae-Hun Lim;Seung-Hoon Lee;Han-Soo Kim
    • Journal of the Computational Structural Engineering Institute of Korea
    • /
    • v.37 no.3
    • /
    • pp.179-186
    • /
    • 2024
  • In this paper, a modification coefficient for equivalent single degree of freedom (SDOF), considering the plasticity range of the member subjected to shock wave type of blast load, was developed. The modification coefficient for the equivalent SDOF was determined through comparison with the analysis of a multi-degree of freedom (MDOF) system. The parameters influencing the equivalent SDOF system analysis were chosen as the boundary conditions of the member and the ratio of the duration of blast load to the natural period of the member. The modification coefficient was calculated based on the elastic load-mass transformation factor. The modification coefficient curve was derived using an elliptical equation to ensure it exists between the upper and lower parameter bounds. Using the modification coefficient on examples with varying cross sections and boundary conditions reduced the SDOF analysis error rate from 15% to 3%. This study shows that using the modification coefficient significantly improves the accuracy of SDOF analysis. The modification coefficient proposed in this study can be used for blast analysis.

A New Detailed Assessment for Liquefaction Potential Based on the Liquefaction Driving Effect of the Real Earthquake Motion (실지진하중의 액상화 발생특성에 기초한 액상화 상세평가법)

  • 최재순;강한수;김수일
    • Journal of the Korean Geotechnical Society
    • /
    • v.20 no.5
    • /
    • pp.145-159
    • /
    • 2004
  • The conventional method for assessment of liquefaction potential proposed by Seed and Idriss has been widely used in most countries because of simplicity of tests. Even though various data such as stress, strain, stress path, and excess pore water pressure can be obtained from the dynamic test, especially, two simple experimental data such as the maximum deviatoric stress and the number of cycles at liquefaction have been used in the conventional assessment. In this study, a new detailed assessment for liquefaction potential to reflect both characteristics of real earthquake motion and dynamic soil resistance is proposed and verified. In the assessment, the safety factor of the liquefaction potential at a given depth of a site can be obtained by the ratio of a resistible cumulative plastic shear strain determined through the performance of the conventional cyclic test and a driving cumulative plastic shear strain calculated from the shear strain time history through the ground response analysis. The last point to cumulate the driving plastic shear strain to initiate soil liquefaction is important for this assessment. From the result of cyclic triaxial test using real earthquake motions, it was concluded that liquefaction under the impact-type earthquake loads would initiate as soon as a peak loading signal was reached. The driving cumulative plastic shear strain, therefore, can be determined by adding all plastic shear strains obtained from the ground response analysis up to the peak point. Through the verification of the proposed assessment, it can be concluded that the proposed assessment for liquefaction potential can be a progressive method to reflect both characteristics of the unique soil resistance and earthquake parameters such as peak earthquake signal, significant duration time, earthquake loading type, and magnitude.

Evolution of Healthcare Service Disparities: A Case Study of Primary Care Services in Korea, 1995-2021 (보건의료 서비스의 공간적 불균등 분포 변이에 대한 연구: 1995년부터 2021년까지 초기진료기관을 대상으로)

  • Hyun Kim;Yena Song
    • Journal of the Economic Geographical Society of Korea
    • /
    • v.26 no.3
    • /
    • pp.289-309
    • /
    • 2023
  • While South Korea's universal healthcare system has garnered attention in public health, the issue of inequality in healthcare service provision among different age groups has incessantly become a significant concern. The focus of this concern is primarily on essential healthcare services, encompassing fundamental aspects of healthcare such as internal medicine, family medicine, and pediatric and adolescent care. This inequality is not limited to differences among age groups (both junior and senior demographics) but also extends to potential disparities in healthcare services based on geographic location, particularly in urban and rural contexts. This paper aims to investigate disparities in primary healthcare service resources in South Korea's evolving economic landscape between 1995 and 2021. We utilize a set of inequality indices with a spatial perspective through geographic cluster analysis. The findings reveal that concerns about inequality have been amplified during various economic events, including the IMF crisis in 1999, the global financial crisis in 2008, and the COVID-19 pandemic in 2020. These years are identified as significant phases that have contributed to manifesting spatial disparities in primary healthcare provisions, with a particular emphasis on the senior-aged population rather than junior or all population groups. Our findings underscore the pressing need to address the unequal distribution of essential healthcare resources as part of preparedness for potential economic impacts, requiring a comprehensive consideration of the interconnected nature of demographic and spatial dimensions in healthcare services.