• Journal of Life Science
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• v.32 no.7
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• pp.523-531
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• 2022

The purpose of this study was to evaluate the effect of antioxidant and anti-adipogenic activities in ethanol extracts from herb mixture (Ephedra sinica, Atractylodes lance, Gypsum fibrosum, and Theobroma cacao). DPPH, ABTS⁺ radical and xanthine oxidase scavenging activities were measured for antioxidant activity. Extracts of the herb mixture had 75.0, 100.8, and 79.5% scavenging activities at 1,000 ㎍/ml concentration, respectively. We investigated the inhibition of adipogenesis and adipocyte differentiation with an extract of an herb mixture in 3T3-L1 preadipocytes. An extract from the herb mixture at concentrations between 0 and 50 ㎍/ml did not affect 3T3-L1 cell viability. Treatment with herb mixture extracts of 25, 50, and 75 ㎍/ml in 3T3-L1 preadipocytes inhibited lipid accumulation in a dose-dependent manner. As a result of a Western blot experiment, it was shown that the herb mixture inhibited the differentiation transcription factors, PPARγ and C/EBPa, by 44.2 and 77.6%, respectively, at a concentration of 75 ㎍/ml in MDI-induced differentiated 3T3-L1 cells. As a result of RT-PCR, the gene expression of C/EBPa, SREBP-1c, and PPARγ was significantly inhibited by 43.4%, 59.6%, and 55.3%, respectively, at the concentration of 75 ㎍/ml of the herb mixture compared with the MDI-treated group. In addition, the expression of fatty acid synthase (FAS), a fatty acid synthesis regulator, was suppressed. These results can be applied to develop a functional food for anti-obesity with a herb mixture.

• Journal of the Korean GEO-environmental Society
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• v.23 no.8
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• pp.29-36
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• 2022

The importance of subsurface information is becoming crucial in urban area due to increase of underground construction. The position of underground facilities should be identified precisely before excavation work. Geophyiscal exporation method such as ground penetration radar (GPR) can be useful to investigate the subsurface facilities. GPR transmits electromagnetic waves to the ground and analyzes the reflected signals to determine the location and depth of subsurface facilities. Unfortunately, the readability of GPR signal is not favorable. To overcome this deficiency and automate the GPR signal processing, deep learning technique has been introduced recently. The accuracy of deep learning model can be improved with abundant training data. The ground is inherently heteorogeneous and the spacially variable ground properties can affact on the GPR signal. However, the effect of ground heterogeneity on the GPR signal has yet to be fully investigated. In this study, ground heterogeneity is simulated based on the fractal theory and GPR simulation is carried out by using gprMax. It is found that as the fractal dimension increases exceed 2.0, the error of fitting parameter reduces significantly. And the range of water content should be less than 0.14 to secure the validity of analysis.

• Journal of Marine Life Science
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• v.3 no.2
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• pp.74-80
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• 2018

Mercury (Hg) poses a threat to marine ecosystem due to continuous inflow from various industries and bioaccumulation to higher trophic level via food web. Mercury can adversely affect growth, development, reproduction and metabolism to aquatic organisms. In the present study, acute toxicity and oxidative stress markers (total glutathione content, and activities of GST, GR and GPx) were investigated in brackish water flea Disphanosoma celebensis exposed to HgCl₂ for 24 h. As results, Hg showed negative effect in survival of D. celebensis. 24 h-LC₅₀ value was determined as 0.589 mg/l (95% C.I. 0.521~0.655 mg/l). After exposure to Hg (0.08 and 0.4 mg/l) for 24 h, total glutathione content was significantly decreased, whereas GST, GPx and GR activities were enhanced. These findings indicate that Hg induced oxidative stress in D. celebensis, and oxidative stress markers may be involved in cellular defense against Hg - mediated toxicity. This study provides a better understanding of molecular mode of action of Hg toxicity in this specie and potent of molecular markers for heavy metal monitoring in marine ecosystem.

• Convergence Security Journal
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• v.23 no.2
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• pp.27-36
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• 2023

In recent years, ransomware attacks have become more organized and specialized, with the sophistication of attacks targeting specific individuals or organizations using tactics such as social engineering, spear phishing, and even machine learning, some operating as business models. In order to effectively respond to this, various researches and solutions are being developed and operated to detect and prevent attacks before they cause serious damage. In particular, honeypots can be used to minimize the risk of attack on IT systems and networks, as well as act as an early warning and advanced security monitoring tool, but in cases where ransomware does not have priority access to the decoy file, or bypasses it completely. has a disadvantage that effective ransomware response is limited. In this paper, this honeypot is optimized for the user environment to create a reliable real-time dynamic honeypot file, minimizing the possibility of an attacker bypassing the honeypot, and increasing the detection rate by preventing the attacker from recognizing that it is a honeypot file. To this end, four models, including a basic data collection model for dynamic honeypot generation, were designed (basic data collection model / user-defined model / sample statistical model / experience accumulation model), and their validity was verified.

• Food Science and Preservation
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• v.30 no.3
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• pp.459-471
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• 2023

The Bayesian network (BN) model was applied to analyze the characteristic variables that affect compliance with safety inspections of farmed eel during the production stage, using the data from 30,063 cases of eel aquafarm safety inspection in the Integrated Food Safety Information Network (IFSIN) from 2012 to 2021. The dataset for establishing the BN model included 77 non-conforming cases. Relevant HACCP data, geographic information about the aquafarms, and environmental data were collected and mapped to the IFSIN data to derive explanatory variables for nonconformity. Aquafarm HACCP certification, detection history of harmful substances during the last 5 y, history of nonconformity during the last 5 y, and the suitability of the aquatic environment as determined by the levels of total coliform bacteria and total organic carbon were selected as the explanatory variables. The highest achievable eel aquafarm noncompliance rate by manipulating the derived explanatory variables was 24.5%, which was 94 times higher than the overall farmed eel noncompliance rate reported in IFSIN between 2017 and 2021. The established BN model was validated using the IFSIN eel aquafarm inspection results conducted between January and August 2022. The noncompliance rate in the validation set was 0.22% (15 nonconformances out of 6,785 cases). The precision of BN model prediction was 0.1579, which was 71.4 times higher than the non-compliance rate of the validation set.

• Food Science and Preservation
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• v.30 no.3
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• pp.502-513
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• 2023

This study aimed to evaluate the anti-adipogenic and anti-inflammation effects of extract from Maclura tricuspidata twig fermented with Ganoderma lucidum mycelium (EMFG) in 3T3-L1 preadipocytes. 3T3-L1 adipocytes were treated with 100, 200, 300 ㎍/mL of EMFG. The result showed that EMFG dose-dependently inhibited the accumulation of intracellular lipid content in differentiated 3T3-L1 adipocytes and enhanced increase of adiponectin release and inhibition of leptin release. EMFG treatment reduced expression of adipogenic transcriptional factor such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT-enhancer-binding protein α (C/EBPα). EMFG also decreased production of lipopolysaccharide (LPS)-induced inflammatory cytokine [tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1)] and the protein expression of cyclooxygenase-2 (COX-2) and inducible NOS (iNOS) in differentiated 3T3-L1 adipocytes. The study demonstrated that EMFG inhibited adipogenesis and inflammation in a dose-dependent manner. These findings suggest that EMFG may have potential as an anti-obesity and anti-metabolic disease agent that works by inhibiting adipogenesis and inflammation.

• Journal of the Korean Society of Marine Environment & Safety
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• v.30 no.2
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• pp.165-175
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• 2024

Hydrogen is being touted as one of the energy sources to combat the climate change crisis. However, hydrogen can leak into enclosed spaces, rise to the ceiling, accumulate, and cause fires and explosions if it encounters an ignition source. In particular, ships that transport hydrogen or use it as a fuel comprise multiple enclosed spaces. Therefore, the dif usion characteristics within these spaces must be understood to ensure the safe use of hydrogen. The purpose of this study is to experimentally determine the diffusion characteristics of helium, which has similar properties to hydrogen, in a closed space on board a ship, and to determine the change in the oxygen concentration along the leakage direction as the air change per hour(ACH) increases to 25, 30, 35, 40, and 45 through CFD simulation. The study, results revealed that the oxygen concentration reduction rate was 2% for leakage in the -z direction and 1% for leakage in the +x and +z directions, and the ventilation time was 15 min 30 s for leakage in the -z direction, 7 min for leakage in the +x direction, and 9 min for leakage in the +z direction, showing that differences existed in the oxygen concentration and ventilation time depending on the leakage direction. In addition, no significant difference was observed in the rate of oxygen concentration reduction and ventilation time in all leakage directions from the ACH of 35 and above in the experimental space. Therefore, because the oxygen concentration and ventilation time were not improved by increasing the ACH, 35 was noted as the optimal ACH in this experimental environment.

• The Korean Journal of Malacology
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• v.32 no.3
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• pp.231-240
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• 2016

Techniques were developed for holding and conditioning of Pacific oysters, Crassostrea gigas, in a closed recirculating system. Experimental adults were used 500 oysters ( x two system, total 1,000 oysters) which were collected in $20^{th}$ March 2016 from long-line aquaculture farm at the south coast of Korea. During conditioning periods concentrated live microalgae as Isochrysis sp. $15{\times}10^7cells/mL$, Tetraselmis sp. $2{\times}10^7cells/mL$ and Pheaodactylum sp. $18{\times}10^7cells/mL$ were added 5 L every day, respectively which micro algae were functioned as diets and biological filter. Over all experimental periods total water exchange rate was 21.3% (daily 0.5%). Over 42 days conditioning, female and male oysters were maturated 90.9% and 94.4%, respectively. Survival rate was 98.7%. Mean shell hight (8.3 mm), total wet weight (19.2 g), meat wet weight (5.0 g) and shell wet weight (13.6 g) were significantly increased (P < 0.05). Water quality parameters including the water temperature ($22.1{\pm}0.4^{\circ}C$), salinity ($24.9{\pm}04$), dissolved oxygen (5.1-7.9 mg/L) and pH ($7.93{\pm}0.15$) were kept stable. Concentration of dissolved inorganic nutrient as ammonia (1.96-0.35 mg/L), nitrite (0.03-0.16 mg/L), nitrate (1.34-0.47 mg/L), DIP (0.42-0.03 mg/L) and silicate (3.83-0.00 mg/L) were significantly decreased throughout experiment except nitrite which was increased (P < 0.05), but nitrogenous components stayed below toxic levels (ammonia 0.0-5.5 mg/L, nitrite 0.0-460.0 mg/L) which indicated that closed recirculation system with microalgae based bio-filter could supply sufficiently environment condition to holding and conditioning of oyster.

• Journal of Aquaculture
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• v.18 no.1
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• pp.26-30
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• 2005

A 10-week feeding trial was conducted to determine the optimum feeding rate of two extruded pellets (EP1 and EP2) and raw fish-based moist pellet (MP) for juvenile flounder. Two replicate groups of the fish (average weight of 27 g) were fed EP and MP three times daily at different feeding rate (satiation and $85\%$ satiation). Weight gain of fish was not affected by diet, but affected by feeding rate. Weight gain with satiation feeding group showed :t tendency to higher compared to that of fish with $85\%$ satiation feeding at the same diet. Feed efficiency of fish was affected by diet, but not feeding rate. Feed efficiency of fish fed the EP1 and EP2 was higher than that of fish fed the MP at the same feeding rate. Daily feed intake was affected by diet and feeding rate. Daily feed intake of fish fed with satiation was higher than that of fish with $85\%$ satiation at same diet, and this variable of fish fed the MP was higher than that of fish fed the EP 1 and EP2. Protein efficiency ratio of fish fed the MP was significantly lower than that of fish fed the EP1 and EP2 regardless of feeding rate. Lipid content of whole body was affected by diet, and that of fish fed the EP2 and MP at satiation feeding was the highest. It can be concluded that satiation feeding will be good and EP is better than MP for growth of juvenile flounder grown from 27 g to 51 g.

• Korean Journal of Environmental Agriculture
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• v.20 no.4
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• pp.203-210
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• 2001

In order to environmentally use wood chips manufactured from low valued forest resources by forest tendering, wood chips were used for the evaluation on chips characteristics, decomposition capability of organic wastes, and field experiment and determination of conditions for decomposer. Bioclusters manufactured by Cryptomeria japonica, commercially available wood chips in Japan, showed higher pore ratio, water reservation and water resistance, and higher cellulose content with lower hot water solubles than domestic wood chips. The useful size of wood chips for swine manure decomposition was 10 (length) ${\times}$ 5 (width) ${\times}$ 2 (thickness) mm, and cellulose contents and alkali solubles of Pinus densiflora and Populus tomentiglandulosa were similar to those of bioclusters. According to the decomposition ratio depending on wood species, it was ordered as Pinus densiflora > Pinus koraiensis > Cryptomeria japonica. The swine manure decomposition ratio depending on treatment hours by Pinus koraiensis was constant with the ratio of 15 to 16 g per hour by 1 kg of chip, indicating of daily swine decomposition amount of 390 kg by 1 ton of chips which was equal to the amount of daily swine manure production by 70 swines. Analyzing by long term used wood chips during 40 days treatment, the treated wood chips characteristically showed stable total nitrogen content, suitable pH, high accumulation of inorganic contents such as calcium, phosphorus, potassium and sodium, and no odor. During winter, the inner temperature of decomposer was kept at $43^{\circ}C$, but air bubble was occurred due to high pH and viscosity of swine manure. The most appropriate mixing ratio between wood chips and swine manure was 1 versus 2 or 3, and at more than ratio 1 versus 3, ammonia gas was caused because of anaerobic fermentation status by high moisture content of wood chips. The mixing interval of decomposer was 3 mins. per hour for the best swine decomposition.