• Journal of Distribution Research
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• v.16 no.5
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• pp.123-140
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• 2011

This study analyzed large distributors' trade features with local suppliers and their direct purchase features through the survey and the interview targeting some MDs(Merchandisers) in large retailers responsible for buying fresh foods (agricultural, fishery, livestock and proceeded products). As the result of conducting the survey targeting the large purchase MDs, it was found that when they select local suppliers, they importantly consider the factors of stable products supply, the quality management, the aggressive management attitude, the affordable (cheap) price, and the suggestion of differentiated goods in order. Concerning their direct purchases in local places, their preference toward the direct-purchase traders were higher than that toward vendors and whole markets as their existing major traders. Among the traders for direct purchase, they preferred farm corporations (including agricultural corporations) the most, and followed the National Agricultural Cooperative, and the unit agricultural cooperative in order. Regarding to the trades with suppliers, when they set the trade price, the merchandisers (MDs) of large distributors preferred long-term trades over 2 years, and utilized the factors of market rate(50.9%), production cost(31.2%) and sale price in the same industry(18.0%). For the local distribution organizations to aggressively counteract the changes of distribution environment in the consumption places, it needs some strategies to organize the agricultural production, to expand the production size and to specialize the distribution channels. And the supply system's continuality and stability are required, so it needs to establish the counteract system enabling to supply all year round through the link of production organizations. Additionally, as an effort to intensify the market negotiation ability, it should collect various market information from the consumption places.

• Journal of Animal Science and Technology
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• v.44 no.6
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• pp.809-816
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• 2002

Chitosan is the deacetylated product of chitin. Chitosan and its derivatives have many properties that make them attractive for a wide variety of health applications. This study was conducted to investigate change of oligosaccharide content and antiviral effect on rotavirus of chitooligosaccharide and water soluble chitosan after heat treatment. The quantitative analysis of oligosaccharide using colorimetry showed that oligosaccharide contents in water soluble chitosan and chitooligosaccharide were decreased from 62.67% to 60.45% and from 59.48% to 54.31%, respectively, after heating. The inhibitory effect of chitosan derivatives on MA-104 cell infected with human rotavirus(HRV) measured using AEC staining method. The inhibition level of 0.125% water-soluble chitosan against cell infection by human rotavirus was 91.98 3.09% in HRV S2 and was 89.92 1.68% in HRV Wa. But, chitooligosaccharide had not shown inhibitory effect against cell infection by HRV. It considered that most oligosaccharide of chitooligosaccharides consist of oligomer of lower polymerization degree. Heat treatment of water soluble chitosan and chitooligosaccharide did not influence their antiviral effects on rotavirus.

• Journal of Animal Science and Technology
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• v.48 no.3
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• pp.467-478
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• 2006

Maintenance of an optimal air quality in the enclosed pig building is potentially important in terms of pig performance and farmer health. The objective of this on-site experiment is to evaluate and compare efficiencies of currently utilized biological additives to reduce odor emissions from the enclosed pig building. As a result, generally all the additives except for salt water, artificial spice and essential oil were proved ineffective in reducing odor generation. The beneficial effects of salt water, artificial spice and essential oil on odor reduction were highlighted on ammonia, odor intensity and offensiveness, and sulfuric odorous compounds, respectively. To efficiently utilize odor masking agent such as the artificial spice, ventilation rate should keep slightly lower than the optimal level. Essential oil functioned well as not only masking agent but also antimicrobial agent for reducing odor. To precisely quantify odor concentration, it should be measured by not the odor sensor but the olfactometry technique.

• Journal of Animal Science and Technology
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• v.45 no.1
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• pp.123-130
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• 2003

Metabolizable energy requirements for maintenance (MEm) of Hanwoo bulls were estimated in twelves metabolism trials using three different feeds at four stages of body weight(100, 200, 300 and 400kg). Three feeds were composed of 1) concentrates and rice straw, 2) concentrates and mixed grass hay, 3) concentrates and corn silage, respectively. Three energy levels were 1) maintenance (M) requirement, 2) 1.5 ${\times}$ M, and 3) 2.0 ${\times}$ M. All bulls were received 60% of their energy from concentrates and 40% form roughages. Three cattle for each trials fed different energy level were housed in metabolism stalls during the 5days of collection period, a total collection of feces and urine. Thereafter, during the 2days of respiration period the heat production was measured by indirect calorimetry using respiratory chamber. MEm were 99.80, 94.48, 94.80, and 97.68 kcal/W0.75 at 100, 200, 300 and 400kg. Mean value of MEm and efficiency of utilization ME for retained energy(Kg) were 95.80 kcal/W0.75 and 0.44.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.307-314
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• 2006

To search direct fed microbials, we isolated a Candida sp. from kefir grain. The isolated Candida sp. strain showed 99.8% of identity to the species of Candida kefyr by API 20C kit. Enzyme activity of Candida kefyr was higher in amylase (0.33±1.12μmol/min/mg) than that in phytase (0.052±0.98μmol/ min/mg) cellulase(0.051±μmol/min/mg) and xylanase (0.011±0.98mol/min/mg). The maximum numbers of Candida kefyr in growth curve were reached at 30 h fermentation. Candida kefyr showed high resistances to acidic environment, which was not perfectly extincted even at pH 2.0. And it showed high tolerance to bile salt which had almost 97.2% of survival in the presence of 1.0% bile salt.Especially, Candida kefyr showed high heat stability which remained 10% of initial microorganisms at 60℃. Candida kefyr was not generally inhibited by most of 11 antibiotic agent which contained tetracycline groups. These results suggest that the isolated Candida kefyr has a useful properties as probiotics.

• Journal of Animal Science and Technology
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• v.51 no.2
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• pp.129-134
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• 2009

The aim of this study was to estimate genetic parameters and annual trends on the racing performance of Thoroughbred horses by a statistical analysis of the resulting records. We used the racing results of 245,979 observations for 13,458 horses recorded in 19 years of race held at Seoul and Busan racing tracks, provided by Korea Racing Authority. After a careful adjustment of some variables such as racing times, jockey and trainer numbers and the average prize a horse won, we selected significant factors that explain the result of racing records of a horse by stepwise AIC and BIC methods. The estimated heritability and repeatability were 0.322 and 0.332, respectively. The average of annual phenotypic and genetic improvement was -0.166 seconds and -0.161 seconds, respectively. Based on the statistical approach, we established reasonable animal model of well-set variables, which is important in the study on estimating performance of racing horses.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.405-410
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• 2012

This study was carried out to investigate the effect of harvest stage of rape on nutritive values and quality of round baled rape silage in field of forage crops of Department of Animal Resources Development, National Institute of Animal Science, RDA. Rape was harvested at three different growth stages (flowering, milk and dough stage) and ensiled at each harvest stages. The content of moisture of rape increased with delayed harvest maturity. However, the content of moisture of rape was controlled by pre-wilting (24 hr. and 48 hr). The content of moisture in dough stage was similar to that of haylage. The content of crude protein in round baled rape silage increased with delayed harvest maturity, but the contents of acid detergent fiber, neutral detergent fiber and total digestible nutrient decreased. The pH in all rape silage ranged from 3.8 to 4.4 at three different harvest stages, and pH in dough stage was higher than that of flowering and milk stages (p<0.05). The content of lactic acid of all rape silage increased with delayed harvest maturity (p<0.05), but the content of acetic acid decreased (p<0.05). And then, flieg's score revealed that there was an increase in order; flowering stage (100) = milk stage (100) > ripen stage (88).

• Journal of Animal Science and Technology
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• v.48 no.5
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• pp.709-718
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• 2006

In order to know the effects of Garlic, Scallion, Flavonoid, Urushiol, Anthocyanidin and Bio-MOS?? on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethylcellulase(CMCase) activity, and microbial populations were investigated.The growth of pathogens was inhibited by supplementation of 0.1% Flavonoid, Scallion or Bio-MOS?? as biological active materials. And Scallion and Flavonoid had powerful antimicrobial properties on the pathogens applied in paper disc method.Although few effects by biological active materials disappeared in rumen fermentation in vitro, CMCase activity removed with supplementation of 1% of Flavonoid which had antimicrobial property in paper disc method. Scallion, having powerful antimicrobial property on pathogens and no inhibiting on rumen fermentation, might be a source in development of natural antimicrobial agent for ruminants.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.731-738
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• 2003

The technique known as proteomics is useful for characterizing the protein expression pattern of a particular tissue or cell type as well as quantitatively identifying differences in the levels of individual proteins. In present study, we carried out the comparative expression patterns of white and red muscles. We used the two-dimensional electrophoresis(2-DE) for analyzing the protein expression. Proteins isolated from porcine white and red muscles were separated by 12% poly-acrylamide gel and then were detected by coomassie blue and silver staining. More than 600 protein spots were detected on each 2-DE gel. By visual analysis of the stained gel, five proteins were identified to be differentially expressed in the white vs red muscle. By database searching based on the molecular weights and pI(isoelectric point) of the five proteins, three of them were found to be most close to troponin I, T and myoglobin. However, further researche is needed for identification and functional analysis of the unidentified proteins. In conclusion, we found five proteins, which are differentially expressed in the white vs red muscle. The functional analysis of the differentially expressed proteins will provide valuable information on biochemical characteristics of the muscle type.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.805-812
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• 2003

This study was conducted to examine effects of feeding dry TMR(DTMR), wet TMR(WTMR) and fermented TMR(FTMR) on rumen fermentation, enzyme activity and digestibility in the total tract of sheep. Three rumen cannulated sheep were used in a 3${\times}$3 latin square design. The present results showed that pH, NH3-N, total and individual VFA, A/P ratio and enzymes (CMCase, Xylanase and Protease) activity in the rumen were higher in WTMR and FTMR compared with DTMR. In addition, dry matter, organic matter, crude protein, ether extract, NDF and ADF digestibility in the total tract were also higher in WTMR and FTMR compared with DTMR. Therefore, the present results showed that WTMR and FTMR are better than DTMR for rumen fermentation and nutrients digestibility.