• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1461-1466
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• 2013

In this study, we ascertained the characteristics of crude protease extracted from fruits (fig, kiwifruit and pineapple) as well as traditional Korean fermentation starters (bio nuruk, traditional nuruk, meju and rice koji) to determine their suitability for industrial application. Crude protease extracted from traditional Korean fermentation starters was found to have a higher optimum temperature ($70^{\circ}C$) and salt concentration (1~3%) but a lower optimum pH (3~6) value compared to the corresponding values for the protease extracted from fruits. On comparison, the total activities of protease per gram unit follow the order: bio nuruk> rice koji> traditional nuruk> fig> pineapple> meju> kiwifruit. Based on our results, we conclude that protease extracted from traditional Korean fermentation starters has potential for application in food industry, for example, as a meat tenderizer for sausage manufacturing and as a protease for cheese production.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.12
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• pp.4922-4927
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• 2010

In order to investigate whether the ethanol extract of Acanthopanax sp. might inhibit herbicide-induced DNA damage and erythrocyte damage, the suppression of the oxidative DNA damage of lymphocyte and erythrocyte damage in the presence of the extract were evaluated by comet assay and hemolysis assay, respectively. Phenoxy herbicides, named 2,4-D (2,4-dichlorophenoxyacetic acid) and 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) and bipyridyl herbicide paraquat induced oxidative DNA damages of lymphocytes. However, the oxidative DNA damage by 2,4-D, 2,4,5-T or paraquat was inhibited in vitro upon treating Acanthopanax extract. Moreover, the erythrocyte damage was also suppressed in vitro by Acanthopanax extract treatment.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.7 no.1
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• pp.17-23
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• 2009

Dihexyloctanamide(DHOA) was used as an extractant or phase modifier with the diamide extractants in a solvent extraction process for a radioactive liquid waste treatment. The degradation compounds of the DHOA extractant, irradiated with $^{60}Co$ gamma ray, were octanoic acid and dihexylamine which are identified by a Fourier transform infrared(FT-IR) and gas chromatograph/mass spectrometer(GC/MS) analysis, and determined by the GC/MS with selected ion monitoring(SIM) mode. Retention behavior of octanoic acid, tridecane (internal standard) and dihexylamine in total ion chromatogram (TIC) were 8.65 min., 9.79 min., and 10.27 min., respectively. With increasing the absorbed dose of the $\gamma$-ray irradiated DHOA, the concentration of octanoic acid was decreased and that of dihexylamine was increased.

• Journal of Life Science
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• v.27 no.9
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• pp.1059-1063
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• 2017

Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful source of novel therapeutic compounds. The balloon-flower has long been used as a traditional medicinal material and food in Asia. In this study, we investigated whether extract of balloon-flower would inhibit in vitro angiogenesis and vascular-like network formation in human umbilical vein endothelial cells (HUVECs). The extract of Balloon-flower did not affect the viability of HUVECs. However, treatment with the Balloon-flower extract suppressed tube formation of HUVECs. In addition, after treatment with the Balloon-flower extract, cell migration decreased about 80%, and cell invasion was almost completely inhibited. Taken together, these results suggest that extract of Balloon-flower may have potential as an angiogenic inhibitor and that it could be developed as an anticancer agent.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.178-182
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• 2005

The concentration of total phenolic compounds of the water and ethanol extracts of Yerusalem sage were 9.4 mg/g and 11.1 mg/g respectively. The total antioxidant activities of water and ethanol extracts of Yerusalem sage were 96.8% and 91.4% in ABTS assay and 97.6% and 87.2% in DPPH assay, PF of the water and ethanolic extracts were 1.1 and 1.6, respectively. TBARS were $0.9{\times}10^{-3}\;{\mu}M$ and $0.3{\times}10^{-3}\;{\mu}M$, respectively. Antimicrobial effect against H. pylori of the ethanol extract was $9{\sim}15\;mm$ of clear zone diameter at $50\;{\mu}g/ml{\sim}200\;{\mu}g/ml$ of phenolic compound. Phenolic profiles in Yerusalem sage chlorogenic aicd was the highest both in water and ethanol extracts in HPLC analyses.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.310-314
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• 2016

The search for natural antioxidants as alternatives to synthetic products is growing. Microalgae have emerged as a source of natural antioxidants with significant and diverse health-promoting properties. In this study, the effects of hot-water extraction conditions on total polyphenol compounds (TPC) production were investigated for lipid extracted microalgae (LEA). In order to enhance the polyphenol productivity, the extraction variables including solvents, temperature, time and ethanol concentration were optimized. The results showed hot-water extraction provided a higher extraction efficiency than the organic solvents and extraction at high temperatures showed a better extraction efficiency. While hot-water extract showed a higher extraction efficiency compared to 98% ethanol extraction, the mixture of water and ethanol (40:60 v/v) showed the highest production of polyphenols. The maximum polyphenols of 3.35 mg GAE (gallic acid equivalent)/g DM were obtained at the optimized extraction time of 10 min, $100^{\circ}C$ and 40% ethanol, respectively. Although Tetraselmis KCTC 12236BP was preprocessed by hexane to remove lipid for bio-diesel production, the results showed LEA contains relatively high level of polyphenols compared to untreated microalgae which can be used in the production of value-added materials. The predictions obtained from the developed Peleg's model were compared with the experimental data under the same operating conditions. The predicted and experimental data were consistent, indicating the reliability of the model.

• Resources Recycling
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• v.14 no.5 s.67
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• pp.18-23
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• 2005

Recovery of phosphoric acid from waste acid mixture of acetic, nitric and phosphoric acid has been attempted by using solvent extraction method. In this work, organic phosphate was used as an extraction agent. The effect of phosphate concentration, agitation speed and time on the solvent extraction of acetic and nitric acids has been investigated. The optimum concentration of phosphate for preferential extraction of acetic and nitric acids from waste acid was found to be about 50% irrespective of agitation speed and time. Purified phophoric acid was recovered from extraction residue at 1/3 of A/O ratio and 6th stage of extraction stage, which is well consistent with the value calculated by using McCabe-Thiele diagram.

• Journal of Mushroom
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• v.11 no.2
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• pp.99-106
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• 2013

This study was initiated to investigate the skin whitening activities of methanol extracts from fruiting bodies of I. obliquus. The total polyphenols and flavonoids contents of I. obliquus methanol extracts were 31.85 mg/g and 28.33 mg/g, respectively. The methanol extract of the mushroom treated on B16/F10 melanoma and NIH3T3 cell lines did not show cytotoxic activity. 2,2-diphenyl-1-picrylhydrazyl(DPPH) free radical scavenging activity and chelating activity on ferrous ions of I. obliquus methanol extract were lower than those of positive control, tocopherol and BHT. The tyrosinase and L-DOPA inhibitory activities of the extract were lower than those of positive control, kojic acid and ascorbic acid. The tyrosinase and melanin synthesis inhibitory activities of the melanoma cells treated with the extract were comparable with positive control, arbutin. The experimental results suggested that methanol extract of I. obliquus contained inhibitory activities of tyrosinase and melanin synthesis in the B16/F10 melanoma cells by dose dependent manner. High ultra-violet absorption spectra in the range of 280-350 nm showed that I. obliquus extract could protect skin from UV radiation damage. Therefore, fruiting bodies of I. obliquus can be used for developing skin whitening, anti-UV and skin care agents.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.26 no.1
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• pp.41-58
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• 2000

Recently the harmfulness of W radiation is in creasing due to encironmental pollution. Environmental population may also play a role in global decrease of ozone layer, A major consequence of ozone depletion is increase in solar ultra violet radiation received at the earth's surface excessive exposure to W radiation cause a lot of problems in our skin. Plant extract that possess antioxidative activities has been reported to retard the oxidation process in product to which they have been added. Plant are alived under solar light. So it is expect the plants have so many protection mechanisms and UV absorbent ingredients against ultra violet radiation such as UVB, UVA. Plant extract which were flavonoids, alkaloids and others could be transformed into UV absorber by chemical modification. Therefore with the aim of finding alternative natural absorber that can safely be used in cosmetic, we have screened various extract for their UV absorbent effect. Thus, the cosmetic safety against human skin, antimicrobial effects and others could be improved by using the silicon.

• Proceedings of the Korea Multimedia Society Conference
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• 2003.05b
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• pp.380-384
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• 2003

현재 정보의 가치가 높아짐에 따라 생체인식에 대한 연구가 많아지고 있다. 특히 지문에 대한 연구가 활발한데 기존의 융선을 이용한 특징점 추출이 아닌 지문에서 잡음이 적은 골선을 이용하고 에지 보존 평활화를 12방향각에 대해 적용함으로 보다 세밀한 에지 보존 평활화를 사용함으로 보다 정확한 특징점 추출의 방법을 제안한다.