• Journal of Ginseng Research
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• v.24 no.1
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• pp.41-45
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• 2000

Maltooligosaccharides were produced from ginseng starch by hydrolysis of $\alpha$-amylase. And it was investigated that physicochemical properties and intestinal bacteria growing effort of maltooligosaccharides. The optimum level of the ginseng maltooligosaccharides was produced when 10% ginseng starch was hydrolyzed with 50 unit of Amano A $\alpha$-amylase per gram starch at 85。C for 24h. Viscosity and water holding capacity of the ginseng maltooligosaccharides were 37.7 cps at 20。C and 110.1% at 75% relative humidity, respectively. The ginseng maltooligosaccharides enhanced the growth of Bifidobaterium infantis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2005.11a
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• pp.141-143
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• 2005

죽절초는 높은 광도 보다는 낮은 광도에서 생육이 건전하게 유지되고 광합성률이 높은 것으로 나타났다. 그러므로 죽절초가 최적으로 생육할 수 있는 조건은 전광의 약 70%정도로 낮은 광도인 실내환경에서 잘 적응할 것으로 추정되어 실내 조경 식물소재로 도입한다면 좋은 관상수가 될 수 있음을 알 수 있었다. 식물 생장의 중요 조건 중 하나인 기공전도도 또한 70% 비음처리구에서 가장 높게 나타나 실내 조경식물로 죽절초가 생육하는 경우에 실내의 낮은 광도에서도 원활한 기공개폐가 이루어져서 실내공기 오염도를 낮추는데 중요한 역할을 할 수 있음을 보여주었다.

• Microbiology and Biotechnology Letters
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• v.30 no.4
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• pp.367-372
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• 2002

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. Optimal medium compositions for production of cytosine deaminase from Chromobacterium violaceum YK 391 were 0.75% soluble starch, 1.5% peptone, 0.1% meat extract, 0.1% yeast extract, 0.01% NaCl, 0.01% $MgCl_2{\cdot}7H_2O$ and 0.05% $K_2HPO_4$. The optimal pH of medium and incubation temperature were 7.0 and $30^{\circ}C$, respectively. C. violaceum reached stationary phase after 30 hr, and produced a maximum cytosine deaminase (120 units/ml) after 72 h in batch culture.

• Journal of Mushroom
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• v.12 no.1
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• pp.35-40
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• 2014

Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and $20^{\circ}C$, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% $(NH_4)_2HPO_4$, 4mM $FeCl_3$, and 3.0% cysteine.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.41-48
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• 2009

This study was conducted to investigate optimum cultural conditions for Amanita hemibapha isolated in Korea and its hyphae characteristics. Micrographs shows the presence of clamp connection. A. hemibapha grows as mycelial form(M-phase) 2-4 ${\mu}m$ and yeast-like form(Y-phase) 7-8 ${\mu}m$. The fungal spores were broadly elliptical and papillate, 8-11 ${\times}$ 6-9 ${\mu}m$ in size. The nucleotide sequence analysis of the ITS of nuclear ribosomal DNA from sporocarps and in-vitro-grown mycelium supported the fungal species is Amanita hemibapha. A. hemibapha showed sequence similarity in the ITS rDNA with A. caesarea(97.5) and A. jacksonii(98.5%) which are morphologically similar species to A. hemibapha. The optimal pH and temperature for mycelial growth of A. hemibapha were pH 6.0 and $28^{\circ}C$, respectively. The fungal species showed best growth in SYP and GYS medium. A. hemibapha grew well with mannitol and glucose as carbon sources and peptone as a nitrogen source.

• The Korean Journal of Mycology
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• v.49 no.1
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• pp.45-55
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• 2021

Artomyces microspora is a genus of coral fungi from the family Auriscalpiaceae that have sporophores which are clavarioid, profusely and pyxidately branched, and devoid of a conspicuous stipe. These fungi can be found in summer and fall. This study aimed to decipher fundamental information regarding optimal growth conditions of Artomyces microsporus mycelia, including pH, temperature, carbon sources, and nitrogen sources. Based on the assessment of colony diameter and mycelial density, the optimal culture medium, temperature, and pH for mycelial growth were found to be PDA, 25 ℃, and pH 5.0, respectively. Furthermore, the study revealed that the optimal carbon and nitrogen sources for mycelial growth were 1% soluble starch and 2% malt extract, respectively. The other suitable inorganic nitrogen sources were deemed to be 0.1% NH₄H₄PO₄ and 0.1% aspartic acid.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.4
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• pp.24-31
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• 1986

A methanol-assimilating yeast for the production of Single Cell Protein was isolated from the soil and identified. Methanol as a sole carbon source was used in this study. The strain was identified as Candida boidinii which grew best at the initial concentration of methanol at 2% , with the addition of 0.5% methanol at every 12 hours, The Single Cell Protein production was maximal after 72 hours of incubation at pH 5.0, $30^{\circ}C$. Thiamin and biotin were stimulated the growth of this yeast at the levels of $1000{\mu}g/{\ell}$ and $10{\mu}g/{\ell}$respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.68-74
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• 1983

A moderately halophilic bacterium, Listeria denitrificans HB-38, isolated from mud on the seashore in Sooyoung bay, Pusan, showed the requirement of $4\%$ sodium chloride for cell growth in a medium with salts typical of a marine environment, and showed that of $10\%$ in a medium with salts typical of a terrestrial environment. The optimum temperature and pH for growth were $40^{circ}C$ and pH 7.5 in the medium containing $10\%$ sodium chloride and ions typical of a terrestrial environment. Sodium chloride as a protoplast stabilizer gave more stability than sorbitol or sucrose, meanwhile the protoplast did not require higher concentration of stabilizer than that of E. coli protoplast. Succinic dehydrogenase of HB-38 had a halophilic property showing maximal activity in the presence of $9\%$ sodium chloride. The strain HB-38 did not harbor an extrach-romosomal DNA.

• Journal of Life Science
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• v.9 no.1
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• pp.111-120
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• 1999

A bacteriocin-producing strain, J-105, was isolated from Kimchi and identified as Lactococcus sp. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For maximum yield of bacteriocin from Lactotoccus sp. J-105, the cell should be harvested at the early stationary phase and temperature, pH and NaCl concentration should be $25^{\circ}C$, pH 8.0 and without the addition of NaCl, respectively. Maltose should be used as a carbon source and organic nitrogen such as polypeptone should be used as a nitrogen source for the best yield. The bacteriocin from isolate was inhibitory against Acetobacter aceti, Bacillus subtilis and several strains of lactic acid bacteria. The bacteriocin of J-105 was sensitive to pepsin, but stable for heat treatment. It was stable even at autoclaving temperature for 15 min.

• Microbiology and Biotechnology Letters
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• v.10 no.4
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• pp.297-305
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• 1982

Environmental conditions of fungal growth on Korean rice grain, Milyang No.23, were studied and optimum treatment conditions of the selected fumigants for controlling fungal deterioration of rice were investigated The results are as follows. 1. Most of the fungi grew well above 80% relative humidity and 19% moisture content within 10-30 days. But at 12.5-13.0% moisture content of grains, only Aspergillus candidus, Asperillus versicolor, Penicillium chrysogenum, and Trichothecium roseum were developed within 2 months. The other fungi were only detectable for their mycelial growth under microscopic observation. 2. Among the ten fumigants tested to control the fungal growth on the rice, ethylene oxide was found to be the most effective at a leve1 of 16 mg/$\ell$ for 48 hrs treatment and the next was methyl bromide (32 mg/$\ell$), acrilonitrile and methyl iodide (64 mg/$\ell$). The other fumigants, such as ammonia, methyl formate, ethyl formate, carbon dioxide and propionic acid were found less effective under the tested conditions.