• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.435-439
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• 2000

Explants of lettuce (Lactuca sativa L.) were cocultured with A. tumefaciens LBA 4404 harboring ${\gamma}$-tocopherol methyltransferase (${\gamma}$-TMT) gene from Arabidopsis thaliana. These explants were transferred to MS medium supplemented with 50 mg/L kanamycin, 500 mg/L carbenicillin, 0.1 mg/L NAA and 0.5 mg/L BA. After 4 weeks, kanamycin resistant shoots were obtained from the explants on the selection medium. The putative transgenic shoots were transferred to rooting MS medium supplemented with 50 mg/L kanamycin and 250 mg/L carbenicillin. Stable incorporation of the Arabidopsis ${\gamma}$-TMT cDNA into lettuce genomic DNA was confirmed by PCR and Southern analysis. HPLC analysis showed that $\alpha$- to ${\gamma}$-tocopherol ratio increased over four fold in a transgenic lettuce line indicating successful expression of the transgenic Arabidopsis ${\gamma}$-TMT in lettuce.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.28 no.2
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• pp.142-147
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• 2015

The artificial light sources for growth of plant are usually high-pressure sodium lamp, metal haloids lamp, and fluorescent light; however, these light sources have relatively weaker Red and Blue lights that are necessary for growth of plants. Especially the effect of Photosynthetic Photon Flux Density (PPFD) is pointed out as the weakness. Meanwhile, LED light source can be selected by specific wavelength to greatly improve the effect of PPFD. In this regard, this paper aims to investigate the promotion of plant growth by measuring photosynthetic photon flux density (hereafter referred to as PPFD) according to changes in light quality of the LED light sources. Towards this end, LED light sources for plant growth were produced with 4 kinds of mono-chromatic lights and 6 kinds of combined lights by mixing red, blue, green and white lights. A comparative analysis was conducted to investigate the effects of optical properties and PPFD on plants (green leaf lettuce) using the produced light sources. The results monochromatic light has fastest growth rate, but plant growth conditions have poor. This being so, mixed light is suitable for the green leaf lettuce.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.456-464
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• 2017

We examined the effect of nutrient solution concentration in the second half of growing period on the pre- and postharvest characteristics of two leaf lettuce cultivars, 'Geokchima' and 'Cheongchima'. Plants were grown hydroponically in a deep flow technique (DFT) system at different concentrations of National Horticulture Research Institute hydroponic nutrient solution: 1/2 strength (S), 1S, 2S, and 4S. Lettuce leaf growth, number of leaves, and shoot fresh weight of both cultivars were greatest in plants grown in the 1S treatment. Compared to other treatments, pigment and nutrient ion contents were greater in the 4S treatment. Growth of lettuce was greatest in the 1S treatment, and decreased at higher or lower concentrations of nutrient solution. However, postharvest characteristics such as fresh weight loss, leaf chlorophyll level, and external appearance were better in both cultivars when grown in 2S solution. Variations in weight loss and SPAD values were smallest in the 2S treatment. These results show that the optimal nutrient concentration for growth does not necessarily provide the optimal postharvest storability.

• Journal of Bio-Environment Control
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• v.16 no.4
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• pp.314-321
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• 2007

This study was conducted to select leaf vegetables suitable for cultivation in apartment verandas and simple and easy fertigation method for home gardening. In order to develop the convenient fertigation method, hydroponics, wick irrigation, and overhead irrigation methods were compared. For the wick irrigation, two types of nutrient sources were used; one was slow release fertilizers mixed with medium and the other one was nutrient solution filled in container located under pots. The growth of leafy lettuce, leaf mustard, and leaf beet was better in both of the wick irrigation methods rather than in overhead irrigation and hydroponics. The wick irrigation method is very easy, so that it is expected to bring a good result from the cultivating and managing point of view, if it brings with commercialized system along with slow release fertilizer. As a result of investigation of environment such as temperature, relative humidity, and irradiance level in apartment verandas in autumn the highest irradiance level during a day was just 48% and 35% in verandas facing south and feeing southeast, respectively, comparing to that in greenhouse. The light environment was investigated as a limiting factor for vegetable growing in verandas. Therefore, to select the vegetables showing good growth under low irradiance environment, nine leaf vegetables such as romaine lettuce, lent lettuce, head lettuce, endive, pak-choi, leaf mustard, garland chrysanthemum, leaf beet, and Chinese chive were grown under 0%, 50%, 70%, 90% shading. Among them, Chinese chive showed the best growth under low irradiance levels. Endive showed line growth reduction according to shading degree, however, even under 90% shading condition, it showed good growth. And then leafy lettuce, garland chrysanthemum, and pak-choi followed. Therefore, these results will be of help in selecting vegetables for veranda gardening with different light levels.

• Food Science and Preservation
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• v.14 no.4
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• pp.345-350
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• 2007

Shelf-life and quality changes of four leaf lettuce cultivars packaged and stored at four different temperatures (0, 8, 16 and $24^{\circ}C$) were evaluated. Leaf lettuces were packaged in non-perforated polypropylene film (thickness 0.05 mm) bags. Weight loss, respiration rate, freshness degree, pigment content, and surface color were determined. At the storage temperature of $0^{\circ}C$, the weight loss, degree of freshness, pigment content, and change of surface color on packaged leaf lettuces showed no significant difference when compared with all other leaf lettuces. Meanwhile, an increase in storage temperature was observed with the quality changes of each flesh leaf lettuce. The leaf lettuces under storage conditions at $24^{\circ}C$ showed a difference in weight loss of the different types of leaf lettuces during a short storage period. However, there was no clear tendency in weight loss in different types of leaf lettuces as compared with each storage temperature. The rate of respiration and the contents of pigments in the four types of leaf lettuces also showed significant difference as the storage temperature increased.

• Journal of Bio-Environment Control
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• v.26 no.4
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• pp.393-401
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• 2017

The nitrate ($NO_3{^-}$) accumulation of hydroponically grown leafy vegetables may increase in the condition of a closed-type plant production system with low light intensity due to low activity of enzymes involved in nitrogen assimilation and the use of $NO_3-N$ as major nitrogen source. The objective of this study is to investigate the effects of light intensities, nutrient solution compositions and the time of nutrient solution removal before harvest on nitrate contents of hydroponically-grown lettuces in a closed plant production system. The reduction of nitrate contents in leafy lettuces 'Cheongchima' was higher in the treatments of 'TW' (nutrient solution removal) and '$(NH_4)_2CO_3$' (use of ammonium carbonate as nitrogen source) than those in other treatments, which significantly lowered fresh weight and leaf area of the plants. In the light intensity of $100{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, the nitrate content was effectively reduced without causing any growth retardation, by substitution of the nutrient solution composition that $NO_3-N$ was removed ('$NO_3-N$ removal' treatment) or the half strength of standard nutrient solution was applied ('1/2 S' treatment), for 7days before harvest. The effects of light intensity and the time of nutrient solution removal before harvest on growth and nitrate contents in leafy lettuces were investigated. The nitrate contents in leaves under the light condition of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ before nutrient solution removal were lower than those of 100 or $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The removal of nutrient solution for 7 days before harvest quickly reduced the amount of nitrates in leaves in all the light intensities with a greater degree under the $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light condition, while the 7 days-removal with both 200 and $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of light conditions caused decrease in 16~31% of leaf area and 20~35% of fresh weight, compared to the 3 days-removal treatment. The nitrate contents were greatly reduced from 3,018 to 1,035 in $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and 2,021 to 480 ppm in the light condition of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, with the nutrient solution removal for 3 days before harvest, without causing any deterioration in growth and product quality. The vitamin C contents in leaves were higher in the treatment of nutrient solution removal for both 3 and 5 days before harvest with the light condition of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ than those in the light condition of 100 or $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$.

• Korean Journal of Organic Agriculture
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• v.21 no.4
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• pp.659-668
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• 2013

The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Chung Chi Ma'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red+blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red+blue and fluorescent light irradiation had a higher $a^*$ value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red+blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red+blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.