• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.22-29
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• 2011

Host plants of Popillia flavosellata (Coleoptera: Ruteridae) were investigated at the courses of three golf clubs, East Valley Golf Club, Anyang Benest Golf Club, and Anseong Benest Golf Club and their surrounded forests. In addition, seasonal occurrence was examined from East Valley Golf Club using Japanese beetle pheromone lure and a eugenol feeding attractant. Twenty six species of 25 genera in 18 families were recorded as hosts of P. flavosellata from given surveyed golf clubs. Out of them, 22 species of 21 genera in 15 families were newly recorded host plants of P. flavosellata. Thus, host plants of P. flavosellata were 49 species of 41 genera in 23 families in total including hosts from literatures. The main damage part of P. flavosellata adult was flower of host plants. Peak activity of P. flavosellata adult was observed around 20 June. Mean numbers of attracted P. flavosellata adults at the peak day were 197.6/trap/day in 2008 and 268.1 in 2010, respectively. Occurrence of P quadriguttata was much lower compared with P. flavosellata at the given golf club. That is, mean numbers of attracted P quadriguttata adults from the same trap at the peak: day were 0.4/trap/day in 2008 and 2010, respectively.

• Journal of Life Science
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• v.25 no.9
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• pp.993-999
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• 2015

The beetle Popillia flavosellata has been no reported its functional effects. In this study, we investigated the anti-inflammatory effect of P. flavosellata ethanol extract (PFE) on RAW 264.7 mouse macrophage cells treated with lipopolysaccharide (LPS) for the induction of inflammation. First, we examined the cytotoxicity of PFE in the RAW 264.7 cells at a concentration of 2,000 μg/ml or less. To evaluate the anti-inflammatory effects of PFE, we investigated the expression levels of proinflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6, and proinflammatory enzymes, such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-induced RAW 264.7 cells. In addition, we examined whether PFE inhibited the translocation of nuclear factor kappa B (NF-κB) p65 into the nucleus in the LPS-induced RAW 264.7 cells. We found that the protein levels of TNF-α and IL-6 were decreased in the LPS-induced RAW 264.7 cells after the treatment with PFE in a dose-dependent manner. In addition, we confirmed that PFE inhibited the translocation of NF-κB p65 into the nucleus, as well as the protein expression levels of iNOS and COX-2. Accordingly, we propose that PFE exerts an anti-inflammatory effect through the down-regulation of NF-κB p65, TNF-α, IL-6, iNOS, and COX-2 via the toll like receptor (TLR)-4 inflammatory signaling pathway.