• The Korean Journal of Mycology
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• v.26 no.4 s.87
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• pp.466-477
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• 1998

Twenty-two Phellinus strains were characterized using colony morphologies and polymerase chain reaction (PCR) to divide into Phellinus linteus. There were some differences in mycelial growth and colony shapes among the strains when they were grown on various media such as PDA, MCM, MEA and YM. Phellinus linteus was slowly growing, formed golden-yellow colony, and produced blue pigment on PDA media. When the regions of internal transcribed spacer (ITS) were amplified from ribosomal RNA (rRNA) coding genes of P. igniarius and P. linteus strains by means of PCR, two types of band (700 bp and 800 bp) were appeared, respectively. For the amplified intergenic region I (IGRI), P. igniarius strains showed a different band among 500, 600, 700 and 800 bp according to the strains, whereas P. linteus strains did one specific band of 700 bp. By polymorphism analysis after digesting the amplified products with 6 different restriction enzymes, a band specific to P. linteus was generated when the products for ITS region were digested with HaeIII, suggesting that the enzyme digestion could provide effective method to distinguish between P. igniarius and P. linteus. And also, the analysis of genetic relationship showed that the genetic similarities were 89% and 95% in P. igniarius and P. linteus strains, respectively. Random amplification polymorphic DNA (RAPD) analysis using multiple primer sets and arbitrarily primed PCR (AP-PCR) with ITS3 primer could also result in a reproducible way to identify P. linteus strains.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.33 no.3
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• pp.110-121
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• 2021

The importance of tidal flats lost due to industrialization has recently received attention, and attention is being paid to the creation of artificial tidal flats and maintenance of natural tidal flats. However, there is still a lack of understanding about the behavioral characteristics of mud, mud, and sand that form tidal flats. Although research on the movement characteristics of mixed soils such as tidal flats has been conducted through field investigations and hydraulic experiments, interest in developing a numerical model based on these results has not yet reached. In this paper, the purpose of this paper is to establish a mixed soil model that can efficiently manage the low quality of the tidal flats. In constructing a model for reproducing the surface movement of mixed soil, the numerical stability of the reproduction and movement of sand and mud constituting the mixed soil in the numerical model should be considered first, so first, the volume of sand and mud constituting the mixed soil A mixed soil model representing the relationship was proposed based on a topographical diagram representing the geometric structure of the mixed soil. In order to consider the dry bulk density of the mixed soil, it was possible to consider the dry bulk density of the mud by introducing the water content of the mud containing water. In addition, it was confirmed that the mud and sand movement calculation according to the slope collapse of the mixed soil was stably performed through the calculation of the slope collapse of the mixed soil through the numerical analysis model to which the proposed mixed soil model was applied.

• Journal of Mushroom
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• v.15 no.4
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• pp.229-236
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• 2017

This study was carried out to investigate the physicochemical characteristics of cultivated cereals by Phellinus linteus mycelium. Also, we investigated the content of free sugar, organic acid, free amino acid, mineral and fatty acid of cultivated cereals by P. linteus mycelium. The major free sugars were determined the glucose, maltose, and fucose by HPLC. The content of total free sugars was the highest in cultivated red rice by P. linteus mycelium at $36^{\circ}C$. The content of fucose was the highest in cultivated red rice by P. linteus mycelium at all cultivation temperatures. The organic acids were identified oxalic acid, malic acid, citric acid, and succinic acid, as in the case of free sugar, higher contents of organic acids were observed in the cultivated cereals by P. linteus mycelium than control. There was no significant difference in the mineral content between the control and cultivated cereals by P. linteus mycelium. Sixteen kind of free amino acid were detected in cultivated cereals by P. linteus mycelium. And the content of total free amino acids were higher in the cultivated cereals by P. linteus mycelium than the control. And essential amino acids showed significantly differences between control and cultivated cereals by P. linteus mycelium. Fatty acids were detected with seven kinds of fatty acids, and the major fatty acid was determined the linoleic acid by GC-MS. The content of linoleic acid was higher in the cultivated cereals by P. linteus mycelium than the control.

• The Korean Journal of Mycology
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• v.41 no.2
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• pp.104-111
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• 2013

Phellinus baumii is a medicinal mushroom used in Asian countries for a long period of time. The purpose of this study was to investigate the skin whitening activities of methanol extracts from fruiting bodies of P. baumii. To evaluate the antioxidant activities of the extract, polyphenol and flavonoid contents, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and chelating activity on ferrous ions were studied. For assay of skin whitening activities, tyrosinase and DOPA inhibitory activities, and tyrosinase and melanin synthesis inhibitory activities of B16/F10 melanoma cells treated with the methanol extract were investigated. The total polyphenol content of P. baumii methanol extract was 4.19. DPPH scavenging ability of P. baumii methanol extract was 88.26% in $25{\mu}g/mL$ concentration. We tested tyrosinase inhibitory activity and melanin contents in melanoma cells. The tyrosinase activity was inhibited to 65.17% at the concentration of $125{\mu}g/mL$ and melanin synthesis was inhibited to 57.61% at the concentration of $25{\mu}g/mL$. Overall, the experimental results showed that P. baumii methanol extract had inhibitory activities of tyrosinase and melanin synthesis by dose dependent manner in B16/F10 melanoma cells. Strong ultra-violet absorption spectra in the range of 270~370 nm indicated that ethanol extract of P. baumii could protect the skin from UV. Therefore, P. baumii methanol extract might be used for development of skin whitening, anti-UV and skin care agents.

• Proceedings of the Korean Geotechical Society Conference
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• 1995.10a
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• pp.15.1-20
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• 1995

일반적으로 일반 및 유해폐기물 매립지에서 진흙층의 기능적 수행목적은 다음과 같다고 할 수 있다. 지표면 유출의 증진을 위하고, 우수의 침투억제 및 폐기물의 표면 노출 시 완충작용, 따라서 최종복토의 붕괴는 이러한 중요 기능을 마비시키고 있다. 본 연구는 추운 날씨상태의 폐기물 매립지 최종복토의 거동을 수행하였으며, 실제로 설계된 세 개의 거대한 실험실 Tank에서 복토에 대한 실험이 시행되었다. 이 거대한 실험장비의 제원은 0.75m x 2.4m와 2.0m 깊이로 되어 있다. Tank안은 시방서적으로 폐기물 매립지가 요구하는 진흙층과 최종복토를 위한 물질로 채워졌다. 그들의 실험결과는 동질/융해에 따른 진흙과 복토층의 붕괴현상은 물리적, 공학적인 측면에 영향을 주는 것으로 나타났다. 본문은 실험에 사용된 복토층의 묘사, 그들의 실험결과들과 실험을 통한 결과분석 및 결론을 설명하고 있다. 실험결과로서는 각 모형들의 우수와 침출수 자료, 온도변화의 배경과 최종복토에서의 Water Balance를 포함하고 있다. 또한 결과로서 시험된 세 개의 모형들은 매립지에서의 동결깊이(29cm~32cm)를 나타내주며 그들 중 하나의 모형은 배수층을 가지고 있어 침출수억제에 효과적이므로 다른 모형보다 복토층으로서 우위에 있다는 것을 지적하고 있다.

• The Korean Journal of Mycology
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• v.30 no.2
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• pp.124-130
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• 2002

The inductions of phase II enzymes, such as NAD(P)H : quinone reductase (QR), glutathione S-transferase (GST), glutathione (GSH) level and the inhibition of polyamine metabolism were tested for the chemopreventive potentials of the extracts from the soybean fermented with Agrocybe cylindracea (AC) or Phellinus igniarius (PI). The soybean fermented with AC or PI was potent inducer of QR activity in murine hepatoma Hepa1c1c7 cells. GST activities of the extracts from soybean fermented with AC or PI were higher than that of the extract from soybean not fermented with basidiomycetes. In addition, GSH levels of the extracts from soybean fermented with AC or PI were increased about 1.2 fold or 1.4 fold, respectively. In addition, proliferation of Acanthamoeba castellanii in a broth medium was inhibited by the extracts from soybean fermented with AC or PI at the both concentration of 20 and 40 mg/3 ml. These results suggest that soybean fermented with AC or PI may have chemopreventive potentials by inducing QR activity, increasing GSH and GST levels and inhibiting polyamine metabolism.

• Monthly Duck's Village
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• s.79
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• pp.82-85
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• 2010

• Korean Architects
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• no.11 s.343
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• pp.18-19
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• 1997

• Bulletin of Korea Environmental Preservation Association
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• v.25 no.3_4
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• pp.47-47
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• 2003

• Monthly Duck's Village
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• s.149
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• pp.74-77
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• 2015