• Title/Summary/Keyword: 진화 기법

Search Result 488, Processing Time 0.029 seconds

Development of EvaGreen Based Real-time PCR Assay for Detection and Quantification Toxic Dinoflagellate Pfiesteria Piscicida and Field Applications (유독 와편모조류 Pfiesteria Piscicida 탐지 및 정량 분석을 위한 EvaGreen 기반 Real-time PCR기법 개발과 현장 적용)

  • PARK, BUM SOO;JOO, JAE-HYOUNG;KIM, MYO-KYUNG;KIM, JOO-HWAN;KIM, JIN HO;BAEK, SEUNG HO;HAN, MYUNG-SOO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
    • /
    • v.22 no.1
    • /
    • pp.31-44
    • /
    • 2017
  • Pfiesteria piscicida is one of heterotrophic dinoflagellate having toxic metaboliges, and it is difficult to detect and quantify this dinoflagellate via light microscope due to small size and morphological similarity with Pfiesteria-like dinoflagellate (PLD) species. Alternatively, we developed quantitative real-time PCR assay based on EvaGreen and determined field accessibility throughout the investigation of distribution in the entire Korean coastal waters and population dynamics in Shihwa Lake. The P. piscicida-specific primers based on internal transcribed spacer 1 (ITS 1) were designed and the specificity of primers was confirmed by PCR with other genomic DNAs which have genetic similarity with target species. Through real-time PCR assay, a standard curve which had a significant linear correlation between log cell number and $C_T$ value ($r^2{\geq}0.999$) and one informative melting peak ($88^{\circ}C$) were obtained. These results implies that developed real-time PCR can accurately detect and quantify P. piscicida. Throughout the field applications of real-time PCR assay, P. piscicida was distributed in western (Mokpo and Kimje) and easthern (Gangneng) Korean coastal water even though light microscopy failed to identify P. piscicida. In the investigation of population dynamics in Shihwa Lake, the density of P. piscicida was peaked in June, July and August 2007 at St. 1 where salinity (${\leq}15psu$) was lower than the other 2 sites. In this study, we successed to develop EvaGreen bassed real-time PCR for detection and quantification of P. piscicida in fields, so this developed assay will be useful for various ecological studies in the future.

Statistically Analyzed Effects of Coal-Fired Power Plants in West Coast on the Surface Air Pollutants over Seoul Metropolitan Area (통계적 기법을 활용한 서해안 화력발전소 오염물질 배출에 따른 수도권 지표면 대기오염농도 영향의 분석)

  • Ju, Jaemin;Youn, Daeok
    • Journal of the Korean earth science society
    • /
    • v.40 no.6
    • /
    • pp.549-560
    • /
    • 2019
  • The effects of the coal-fired power plant emissions, as the biggest point source of air pollutants, on spatiotemporal surface air pollution over the remote area are investigated in this study, based on a set of date selection and statistical technique to consider meteorological and geographical effects in the emission-concentration (source-receptor) relationship. We here proposed the sophisticated technique of data processing to separate and quantify the effects. The data technique comprises a set of data selection and statistical analysis procedure that include data selection criteria depending on meteorological conditions and statistical methods such as Kolmogorov-Zurbenko filter (K-Z filter) and empirical orthogonal function (EOF) analysis. The data selection procedure is important for filtering measurement data to consider the meteorological and geographical effects on the emission-concentration relationship. Together with meteorological data from the new high resolution ECMWF reanalysis 5 (ERA5) and the Korea Meteorological Administration automated surface observing system, air pollutant emission data from the telemonitoring system (TMS) of Dangjin and Taean power plants as well as spatio-temporal air pollutant concentrations from the air quality monitoring system are used for 4 years period of 2014-2017. Since all the data used in this study have the temporal resolution of 1 hour, the first EOF mode of spatio-temporal changes in air pollutant concentrations over the Seoul metropolitan area (SMA) due to power plant emission have been analyzed to explain over 97% of total variability under favorable meteorological conditions. It is concluded that SO2, NO2, and PM10 concentrations over the SMA would be decreased by 0.468, 1.050 ppb, and 2.045 ㎍ m-3 respectively if SO2, NO2, and TSP emissions from Dangjin power plant were reduced by 10%. In the same way, the 10% emission reduction in Taean power plant emissions would cause SO2, NO2, and PM10 decreased by 0.284, 0.842 ppb, and 1.230 ㎍ m-3 over the SMA respectively. Emissions from Dangjin power plant affect air pollution over the SMA in higher amount, but with lower R value, than those of Taean under the same meteorological condition.

Density Evolution Analysis of RS-A-SISO Algorithms for Serially Concatenated CPM over Fading Channels (페이딩 채널에서 직렬 결합 CPM (SCCPM)에 대한 RS-A-SISO 알고리즘과 확률 밀도 진화 분석)

  • Chung, Kyu-Hyuk;Heo, Jun
    • Journal of the Institute of Electronics Engineers of Korea TC
    • /
    • v.42 no.7 s.337
    • /
    • pp.27-34
    • /
    • 2005
  • Iterative detection (ID) has proven to be a near-optimal solution for concatenated Finite State Machines (FSMs) with interleavers over an additive white Gaussian noise (AWGN) channel. When perfect channel state information (CSI) is not available at the receiver, an adaptive ID (AID) scheme is required to deal with the unknown, and possibly time-varying parameters. The basic building block for ID or AID is the soft-input soft-output (SISO) or adaptive SISO (A-SISO) module. In this paper, Reduced State SISO (RS-SISO) algorithms have been applied for complexity reduction of the A-SISO module. We show that serially concatenated CPM (SCCPM) with AID has turbo-like performance over fading ISI channels and also RS-A-SISO systems have large iteration gains. Various design options for RS-A-SISO algorithms are evaluated. Recently developed density evolution technique is used to analyze RS-A-SISO algorithms. We show that density evolution technique that is usually used for AWGN systems is also a good analysis tool for RS-A-SISO systems over frequency-selective fading channels.

A Study of Development of Transmission Systems for Next-generation Terrestrial 4K UHD & HD Convergence Broadcasting (차세대 지상파 4K UHD & HD 융합방송을 위한 전송 시스템 개발에 관한 연구)

  • Oh, JongGyu;Won, YongJu;Lee, JinSub;Kim, YongHwan;Paik, JongHo;Kim, JoonTae
    • Journal of Broadcast Engineering
    • /
    • v.19 no.6
    • /
    • pp.767-788
    • /
    • 2014
  • The worldwide transition from analog to digital broadcasting has now been completed and the need to study next generation standards for Ultra High Definition TV (UHDTV) broadcasting, and broadcasting & communication convergence systems is rapidly growing. In particular, high resolution mobile broadcasting services are needed to satisfy recent consumers. Therefore, the development of highly efficient convergence broadcasting systems that provide fixed/mobile broadcasting through a single channel is needed. In this paper, a service scenario and requirements for providing 4K UHD & HD convergence broadcasting services through a terrestrial single channel are analyzed by employing the latest transmission and A/V codec technologies. Optimized transmission parameters for 6 MHz & 8 MHz terrestrial bandwidths are drawn, and receiving performances are measured under Additive White Gaussian Noise (AWGN) and time-varying multipath channels. From the results, in a 6 MHz bandwidth, the reliable receiving of HD layer data can be achieved when the receiver velocity is maximum 140 Km/h and is not achieved when the velocity is over 140 Km/h due to the limit of bandwidth. When the bandwidth is extended to 8 MHz, the reliable receiving of both 4K UHD and HD layer data is achieved under a very fast fading multipath channel.

Expression Factors of Pace and Dynamics in Drawing Animation - Focused on Japanese Hero TV Animation Series - (드로잉 애니메이션에서 속도감과 역동성의 표현 요소 연구 - 일본 초인물 TV 애니메이션 중심으로-)

  • Kim, Hyun-Woo;Kim, Jae-Woong
    • Cartoon and Animation Studies
    • /
    • s.40
    • /
    • pp.109-137
    • /
    • 2015
  • As Digital technology developed, drawing animation, using traditional production method which expresses many feelings with drawn lines loses the youth group, the primary audience, to the realism of the digital cinema. Drawing animation, which drew attention of the youth by the spectacles in the film such as hectic pace, dynamics and punch, declined for a while. However, it has been developed a way to express pace and dynamics of its own by establishing an effective directing method, which combines digital technology as it is needed. This study has a purpose to investigate what causes the dynamics and feeling of fast movement of the character in Japanese limited animation. Though some action-animated films that heroes with supernatural powers take the leading role that feeling of velocity and dynamics are emphasized we compare the directing method before and after the introduction of the digital technology. This research reaches the conclusion by factoring each Bergson and McLuhan's discussion to the intervention of indexical signs and the audience's participation according to skipping technique. This study has a significance of researching the element of drawing animation that maximizes the expansion of the senses by defying the limitation of the law of physics through its unique way of directing together with growth of the hero films, which will continue.

A Method to Manage Faults in SOA using Autonomic Computing (자율 컴퓨팅을 적용한 SOA 서비스 결함 관리 기법)

  • Cheun, Du-Wan;Lee, Jae-Yoo;La, Hyun-Jung;Kim, Soo-Dong
    • Journal of KIISE:Software and Applications
    • /
    • v.35 no.12
    • /
    • pp.716-730
    • /
    • 2008
  • In Service-Oriented Architecture (SOA), service providers develop and deploy reusable services on the repositories, and service consumers utilize blackbox form of services through their interfaces. Services are also highly evolvable and often heterogeneous. Due to these characteristics of the service, it is hard to manage the faults if faults occur on the services. Autonomic Computing (AC) is a way of designing systems which can manage themselves without direct human intervention. Applying the key disciplines of AC to service management is appealing since key technical issues for service management can be effectively resolved by AC. In this paper, we present a theoretical model, Symptom-Cause-Actuator (SCA), to enable autonomous service fault management in SOA. We derive SCA model from our rigorous observation on how physicians treat patients. In this paper, we first define a five-phase computing model and meta-model of SCA. And, we define a schema of SCA profile, which contains instances of symptoms, causes, actuators and their dependency values in a machine readable form. Then, we present detailed algorithms for the five phases that are used to manage faults the services. To show the applicability of our approach, we demonstrate the result of our case study for the domain of 'Flight Ticket Management Services'.

Program Analysis Activities for Development of Componentization Methodology for of Legacy System (레가시 시스템의 컴포넌트화 방법론 개발을 위한 프로그램 분석 활동)

  • Cha, Jung-Eun;Kim, Cheol-Hong;Yang, Young-Jong
    • Proceedings of the Korea Information Processing Society Conference
    • /
    • 2002.11c
    • /
    • pp.2119-2122
    • /
    • 2002
  • 기업의 비즈니스 프로세스가 복잡, 다양해짐에 따라, 현재 운영 시스템에 대한 급격한 기술적 변화를 수용하고 이를 조직적 측면의 기업 프로세스로 적용하기 위해 레가시 시스템의 현대화가 요구된다. 따라서, 현재의 기업들은 다양한 사용자들이 각자 그들의 관점에서 필요한 비즈니스 요구들을 웹 상에서 처리시킬 수 있도록 J2EE, .NET 등으로 대표되는 컴포넌트 및 웹 서비스 기술을 적용한 새로운 e-business 환경을 수용해야만 한다. 하지만, 기업 조직의 중요한 지식과 프로세스들을 처리하는 시스템들은 대부분 과거(Legacy)의 기술에 의해 개발되어졌으며, 이러한 시스템들을 새로운 비즈니스 환경에 적용하기에는 웹 환경을 위한 분산 아키텍쳐의 결여와 개방성과 표준화 미흡으로 시스템의 유지보수에 많은 어려움을 가진다. 또한, 방법론 차원에서 재공학의 절차와 기법을 체계적으로 정의하고 지원하기 위한 노력이 매우 부족한 실정이다. 본 논문에서는 레거시 시스템을 새로운 시스템 환경으로의 변환 및 통합을 위한 재공학 방법론 개발을 목적으로 프로그램 분석 활동을 설명한다. 본 논문에서 개발하고자 하는 방법론은 다양한 추상화 수준에서 역공학 정보를 복구하고, 컴포넌트화 단계를 통해 새로운 시스템으로 진화할 수 있는 절차 및 기법들을 제공한다. 레거시 시스템 컴포넌트화 방법론은 변환계획 단계, 역공학 단계, 컴포넌트화 단계, 인도 단계의 4 단계로 구성되어 있으며, 본 논문에서는 전체 단계 중 가장 기초가 되고 중요한 단계인 역공학 단계에 초점을 두고 프로그램 분석을 위한 절차 및 과정의 주요 지침들을 제시한다.0보다 유의적으로 우수하였으며, 맛, 다즙성 및 전체적인 기호도는 TMR-0 및 TMR-1 사이에 유의성이 없었다(p<0.05).能性)을 알아보고자 본(本) 실험(實驗)을 실시(實施)한 결과(結果), 유의성(有意性) 있는 결과(結果)를 얻었기에 보고(報告)하는 바이다.또한 이들 상피세포(上皮細胞)들을 투과전자현미경적(透過電子顯微鏡的)으로 관찰(觀察)하였을 때 초미세구조(超微細構造)가 잘 보존(保存)되었으나 Langer-hans cell내(內)의 Birbeck granule은 유리전(遊離前) 피부상피조직내(皮膚上皮組織內)의 Langerhans cell내(內)의 Birbeck granule에 비(比)해 수적(數的)으로 현저히 감소(減少)되어 있었다. 그러나 Thy-1 양성(陽性) dendritic cell에서 볼 수 있는 dense-core 과립(顆粒)은 별변화(別變化)없이 쉽게 관찰(觀察)될 수 있었다. 조직배양(組織培養)을 한 견(犬)의 keratinocyte에 대(對)해 사람 pemphigus vulgaris의 항체(抗體)로 반응(反應)시킨 후 protein-A gold(15 nm)로 표식(標識)시킨 바 제일 바깥 상층(上層)의 keratinocyte에 있어서 세포막표면(細胞膜表面)을 따라 표식(標識)되어 세포막항원(細胞膜抗元)을 나타내었으며, 이와 같은 소견(所見)으로 미루어 정상피부(正常皮膚) 중층편평상피세포(重層扁平上皮細胞)에서도 동일(同一)한 소견(所見)을 관찰(觀察)할 수 있다고 본다.al remnants, Resorption of fetus로 관찰된 것이다. Fetal death는 수정후

  • PDF

An Event-Driven Dynamic Monitor for Efficient Service Monitoring (효율적인 서비스 모니터링을 위한 이벤트 주도 동적 모니터)

  • Kum, Deuk-Kyu;Kim, Soo-Dong
    • Journal of KIISE:Software and Applications
    • /
    • v.37 no.12
    • /
    • pp.892-908
    • /
    • 2010
  • Services in SOA are typically perceived as black-box to service consumers, and can be dynamically evolved at runtime, and run on a number of unknown and heterogeneous environments. Because of these characteristics of the services, effective and efficient monitoring of various aspects on services is an essential functionality for autonomous management of service. But the problem with or limitation in conventional or existing approaches is, that they focus on services themselves, ignoring the effects by business processes. Consequently, there is a room for service monitoring which provides more useful information of business level by acquisition of only external monitoring data that depend on specific BPEL engine and middleware. Moreover, there is a strong demand to present effective methods to reduce monitoring overhead which can degrade quality of services. EDA can cope with such limitations in SOA by collecting and analyzing events efficiently. In this paper, we first describe EDA benefits in service monitoring, and classify monitorring target, and present an appropriate monitoring method for each monitoring target. Also to provide the applicability of our approach, an event meta-model is defined, and event processing model and architecture based on the meta-model are proposed. And, with the proposed architecture and method, we implement a prototype of an event-driven dynamic monitoring framework which can collect and process internal and external data at runtime. Finally, we present the result of a case study to demonstrate the effectiveness and applicability of the proposed approach.

Cost Effective Remote Subscription scheme for Ubiquitous Convergence IP-based Network (유비쿼터스 컨버젼스 IP 기반 대용량 네트워크에서 비용 절감형 리모트-서브스크립션 기법)

  • Shin, Soo-Young;Yoon, Young-Muk;Park, Soo-Hyun
    • The KIPS Transactions:PartC
    • /
    • v.14C no.1 s.111
    • /
    • pp.95-104
    • /
    • 2007
  • Mobile multimedia services such as TV-call or video streaming are gradually becoming popular in the 3rd or more generation mobile network (IMT-2000). Multimedia traffic is expected to continue increasing into the coming years, and IP technology is considered to be the efficient way of transporting such huge volumes of multimedia traffic. IP-based IMT network platform represents an evolution from IMT-2000. The structure of IP-based IMT network as ubiquitous platform is three-layered model : Middleware including Network Control PlatForm (NCPF) and Service Support PlatForm (SSPF), IP-BackBone (IP-BB), access network including sensor network. Mobility Management (MM) architecture in NCPF is proposed for IP-based IMT network in order to manage routing information and location information separately. The generous existing method of multicast control in IP-based IMT network is Remote Subscription. But Remote Subscription has problem that should be reconstructed whole multicast tree when sender in multicast tree moves to another area. To solve this problem, we propose the way to put Multicast-manager in NCPF.

A Trustworthiness Improving Link Evaluation Technique for LOD considering the Syntactic Properties of RDFS, OWL, and OWL2 (RDFS, OWL, OWL2의 문법특성을 고려한 신뢰향상적 LOD 연결성 평가 기법)

  • Park, Jaeyeong;Sohn, Yonglak
    • Journal of KIISE:Databases
    • /
    • v.41 no.4
    • /
    • pp.226-241
    • /
    • 2014
  • LOD(Linked Open Data) is composed of RDF triples which are based on ontologies. They are identified, linked, and accessed under the principles of linked data. Publications of LOD data sets lead to the extension of LOD cloud and ultimately progress to the web of data. However, if ontologically the same things in different LOD data sets are identified by different URIs, it is difficult to figure out their sameness and to provide trustworthy links among them. To solve this problem, we suggest a Trustworthiness Improving Link Evaluation, TILE for short, technique. TILE evaluates links in 4 steps. Step 1 is to consider the inference property of syntactic elements in LOD data set and then generate RDF triples which have existed implicitly. In Step 2, TILE appoints predicates, compares their objects in triples, and then evaluates links between the subjects in the triples. In Step 3, TILE evaluates the predicates' syntactic property at the standpoints of subject description and vocabulary definition and compensates the evaluation results of Step 2. The syntactic elements considered by TILE contain RDFS, OWL, OWL2 which are recommended by W3C. Finally, TILE makes the publisher of LOD data set review the evaluation results and then decide whether to re-evaluate or finalize the links. This leads the publishers' responsibility to be reflected in the trustworthiness of links among the data published.