• KOREAN POULTRY JOURNAL
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• v.40 no.1 s.459
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• pp.229-231
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• 2008

• The Korean Journal of Cytopathology
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• v.10 no.2
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• pp.121-126
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• 1999

The usefulness of E-cadherin immunostaining as a marker of malignancy in the body fluids was investigated in the present study. Thirty-three histologically proven cases of cell blocks from the pleural, peritoneal, and pericardial fluids were studied by immunocytochemistry for E-cadherin antibody using LSAB method. These cases were cytologically diagnosed as adenocarcinoma (25 cases) and atypical cells (8 cases). Tumor cells showed strong positive membranous staining for E-cadherin antibody in 21 out of 25 cases (84%) of adenocarcinoma. E-cadherin staining was not found in 6 of 8 cases of suspicious maligancy. The sensitivity and specificity were 84% and 75%, respectively. Reactive mesothelial cells and Inflammatory cells scattered were all negative. In conclusion, E-cadherin is an useful adjunctive marker to distinguish reactive mesothelial cells from the carcinoma cells in the body fluids.

• Tuberculosis and Respiratory Diseases
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• v.45 no.5
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• pp.1031-1038
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• 1998

Background: Most of malignant pleural effusions are serous but 8-33% of them are bloody. We wanted to evaluate the relationships between gross appearance and pleural CEA level or results of histocytology in malignancy associated pleural effusions. We also tried to reevaluate the meaning of CEA measurement in histocytologically proved or unproved malignancy associated pleural effusions. Methods: We studied 98 cases of malignancy associated pleural effusions, 50 cases of histocytologically proven malignant effusions and 48 cases of histocytologically unproven paramalignant effusions. We had observed gross appearance and conventional laboratory values and CEA levels for pleural effusions. Results: 44.9% of malignancy associated effusions were bloody(63.6% of bloody effusions were histocytologically proven malignant effusion). 65.0% of malignancy associated pleural effusions which have RBCs numbers over $100,000/mm^3$ were cytologically proven malignant effusions. 72.7% of cytologically proven malignant effusions had increased pleural fluid CEA level over 10 ng/ml. 58.2% of cases with pleural CEA over 10 ng/ml had positive results in pleural bistocytology. There was no definable relationships between pleural fluid CEA elevation and RBCs numbers and results of pleural fluid cytology. Conclusion: About half of the cases with malignancy associated pleural effusions were bloody. Histocytologically proven malignant effusions were more common in bloody effusion than non-bloody effusion(63.6% Vs 38.9%). But increased red blood cell numbers was not associated with positivity of pleural histocytology. Pleural fluid CEA elevation(over 10 ng/ml) was not correlated with positive pleural histocytology. But pleural fluid CEA elevation was rare in nonmalignant pleural effusions, and than pleural CEA measurement in uncertain pleural effusions maybe helpful to distinguishes its origin.

• Journal of the Korean Society of Radiology
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• v.81 no.1
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• pp.147-156
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• 2020

Purpose The objective of this study was to evaluate the diagnostic value and threshold levels of cytokeratin fragment 21-1 (CYFRA 21-1) in fine-needle aspiration (FNA) washouts for detection of lymph node (LN) recurrence in postoperative breast cancer patients. Materials and Methods FNA cytological assessments and CYFRA 21-1 measurement in FNA washouts were performed for 64 axillary LNs suspicious for recurrence in 64 post-operative breast cancer patients. Final diagnosis was made on the basis of FNA cytology and follow-up data over at least 2 years. The concentration of CYFRA 21-1 was compared between recurrent LNs and benign LNs. Diagnostic performance and cut-off value were evaluated using a receiver operating characteristic curve. Results Regardless of the non-diagnostic results, the median concentration of CYFRA 21-1 in recurrent LNs was significantly higher than that in benign LNs (p < 0.001). The optimal diagnostic cut-off value was 1.6 ng/mL. The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of CYFRA 21-1 for LN recurrence were 90.9%, 100%, 100%, 98.1%, and 98.4%, respectively. Conclusion Measurement of CYFRA 21-1 concentration from ultrasound-guided FNA biopsy aspirates showed excellent diagnostic performance with a cut-off value of 1.6 ng/mL. These results indicate that measurement of CYFRA 21-1 concentration in FNA washouts is useful for the diagnosis of axillary LN recurrence in post-operative breast cancer patients.

• Research in Plant Disease
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• v.23 no.4
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• pp.306-313
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• 2017

Recently fire blight occurred in the Republic of Korea and eradication program for the disease has been executed since then. Specificity and detection sensitivity of the 2 antibody-based diagnostic strips to Korean isolates of Erwinia amylovora (Ea) and their application for on-site diagnosis were evaluated in this study. Ea AgriStrip, a commercial diagnostic kit, and EB strip, developed in this study, reacted positively to the all tested Korean Ea strains and also to most of Erwinia pyrifoliae (Ep) strains causing black shoot blight. They reacted negatively to all Pusedomonas syringae pv. syringae (Pss) strains that cause shoot blight on apple. Detection sensitivity was similar between the 2 strips. For on-site diagnosis, the two strips reacted positively only to the extractions of the fire-blighted samples on all fire blight occurred orchards except one orchard at which on-site diagnosis was carried out at winter time. In addition, they reacted positively to the black-shoot blighted extractions from the black shoot blight occurred apple orchard. These results suggest that both EB strip and Ea AgriStrip would be useful for on-site diagnosis of fire blight in Korea.

• Proceedings of The Korean Society of Health Promotion Conference
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• 2005.11a
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• pp.234-243
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• 2005

상당히 성공적인 사람병원체인 결핵균을 Robert Koch가 발견한 이래, 결핵 방제의 상당한 진보에도 불구하고, 결핵의 발생은 현재까지 계속되어오고 있다(Tiruviluamala and Reichman 2002). 그러므로, 완전한 민감성과 특이성을 갖는 신속한 진단방법이 결핵과 결핵의 잠복감염을 진단하는데 요구되어지며(Tiruviluamala and Reichman 2002), 계속적으로 결핵균과 BCG의 유전체와 단백질체 연구를 통하여 결핵 방제수단이 발전되어질 것이다 (Jungblut et al. 1999). 본 연구는 PPD가 결핵을 진단하는 특이한 항원만으로 정확하게 조화를 이루고 있지 않다는 것을 보여주었으며, 그것의 역가는 결론적으로 생물학적으로 평가되어져야 하지만, PPD의 제조는 단백질체 분석 기법에 의해 분석되고 표준화될 수 있음을 보여주었다. 이를 통하여 결핵 피내진단의 일괄된 결과를 위해 PPD 사용 전에 이와같은 단백질체 분석기법을 적용할 수 있을 것으로 사료되며, 본 연구에서 밝혀진 특이 항원은 새로운 피내진단항원으로 적용될 수 있을 것으로 사료된다.

• Proceedings of the KIEE Conference
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• 2007.07a
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• pp.1546-1547
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• 2007

휴대용 면역진단 시스템을 구현하기 위하여 설계된 공기주입기로 구동되는 랩온어칩 내의 유체 유동을 컴퓨터 시뮬레이션을 통하여 해석하고, 문제점을 보완할 수 있는 구조로 랩온어칩을 재설계하였다. 공기주입기에서 흘러나오는 공기를 이용해 완충액 저장고 내에 있는 완충액을 토출시킬 때 다량의 기포가 발생함을 시뮬레이션 결과를 통해 알 수 있었다. 완충액 저장고의 내부에 계단형 구조를 삽입함으로서 완충액 이송 시 형성되는 기포를 상당히 억제할 수 있었다. 또한 계단형 구조는 유선을 역행 방지판 쪽으로 분산시켜 역행 방지판의 효율을 높일 것이다.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.4
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• pp.243-250
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• 2015

A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.

• 프린팅코리아
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• v.14 no.7
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• pp.88-89
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• 2015

대한인쇄문화협회(회장 조정석)는 한국콘텐츠진흥원의 지원으로 품질표준화와 친환경 인쇄를 위한 PUR 접착제, 인쇄세척액, 한자서체, 인쇄품질 공정진단 프로그램 개발 등 4개 부문의 연구개발 과제를 성공적으로 완료했다.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.12a
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• pp.122-122
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• 2004