• Title/Summary/Keyword: 직접접합

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The Amount of Telomeric DNA and Telomerase Activity on Cattle Cells (소의 생리적 특성에따름 세포내 텔로미어 함량과 텔로머레이스 활성도 분석)

  • Choi, Duk-Soon;Cho, Chang-Yeon;Sohn, Sea-Hwan
    • Journal of Animal Science and Technology
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    • v.50 no.4
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    • pp.445-456
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    • 2008
  • Telomeres consist of TTAGGG tandem repeated DNA sequences with specific proteins and locate at chromosome ends. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Telomerase is a ribonucleoprotein which has a template for the synthesis of telomeric DNA. This study was carried out to analyze the amount of telomeric DNA and telomerase activity in cattle cells. Analysis of the quantity of telomere in lymphocytes was done at different ages, sex and among Korean cattle and Holstein breeds. The telomerase activity was also analyzed in liver, brain, heart, kidney, and testis tissues of fetal calf and of 18 month old cattle. The amount of telomeres in lymphocytes and other tissue cells was analyzed by Quantitative-Fluorescence in situ Hybridization (Q-FISH) technique using a telomeric DNA probe. Telomerase activity was analyzed by Telomeric Repeat Amplification Protocol assay (TRAP). The amount of telomeric DNA on the lymphocytes during the whole life span was decreased along with age. Quantity of telomeres in Korean cattle was significantly higher than that in Holstein breed. The amount of telomeric DNA in males was significantly higher than that in females. Telomerase activity was up-regulated in most bovine tissues during fetal stage, but was down-regulated in most tissues at mature 18 month age except the testis cells. This study indicates that the amount of telomeres and telomerase activity of cells can be used as an age marker or/and a physiological marker of cattle.

Immediate Connection of Customized Zirconia Abutment Using Flapless Guided Surgery: A Clinical Report (무절개 수술을 이용한 맞춤형 지르코니아 지대주의 즉시 장착 증례)

  • Lee, Gyeong-Je;Choi, Byung-Ho;Kim, Hee-Jun;Jung, Seng-Mi
    • Journal of Dental Rehabilitation and Applied Science
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    • v.28 no.2
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    • pp.201-212
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    • 2012
  • There are some similar aspects at histological and morphological characteristics between the peri-implant tissue and periodontal tissue and the direct attachment between the titanium and soft tissue around the implant called as "Functional ankylosis" can prevent the apical infiltration of inflammatory and bone resorption around implant. But, the repeated connection and disconnection of the abutment can destroy the mucosal barrier of soft tissue around the implant and can cause the marginal bone resorption. The amount of marginal bone resorption may reduced if the prosthetic abutment is placed at that time of surgery. Connection of the prosthetic abutment at surgery was limited because the low accuracy of conventional method, but by using of Cone Beam Computed Tomography(CBCT) and guide surgery, the 3-dimensional accuracy of implant placement became much higher than before and it became possible. This is a clinical case of immediate connection of prosthetic abutment and provisional restoration by using of precise CBCT diagnosis and pre-fabricated zirconia customized abutment at surgery and the alternative method is described in this article because of the clinically contentable results.

Seam-line Determination in Image Mosaicking using Adaptive Cost Transform and Dynamic Programming (동적계획법과 적응 비용 변환을 이용한 영상 모자이크의 seam-line 결정)

  • Chon, Jae-Choon;Suh, Yong-Cheol;Kim, Hyong-Suk
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.2
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    • pp.16-28
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    • 2004
  • A seam-line determination algorithm is proposed to determine image border-line in mosaicing using the transformation of gray value differences and dynamic programming. Since visually good border-line is the one along which pixel differences are as small as possible, it can be determined in association with an optimal path finding algorithm. A well-known effective optimal path finding algorithm is the Dynamic Programming (DP). Direct application of the dynamic programming to the seam-line determination causes the distance effect, in which seam-line is affected by its length as well as the gray value difference. In this paper, an adaptive cost transform algorithm with which the distance effect is suppressed is proposed in order to utilize the dynamic programming on the transformed pixel difference space. Also, a figure of merit which is the summation of fixed number of the biggest pixel difference on the seam-line (SFBPD) is suggested as an evaluation measure of seamlines. The performance of the proposed algorithm has been tested in both quantitively and visually on various kinds of images.

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The Mechanical Sensitivity at Interfaces between Bone and Interbody Cage of Lumbar Spine Segments (Lumbar spine 의 뼈와 Interbody cage의 접촉면에서 기계공학적 민감성 고찰)

  • Kim Y.
    • Journal of Biomedical Engineering Research
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    • v.21 no.3 s.61
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    • pp.295-301
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    • 2000
  • It is known that among many factors, relative micromotion at bone/implant interfaces can hinder bone ingrowth into surface pores of an implant. Loading conditions, mechanical properties of spinal materials, friction coefficients at the interfaces and geometry of spinal segments would affect the relative micromotion and spinal stability. A finite clement model of the human lumbar spine segments (L4-L5) was constructed to investigate the mechanical sensitivity at the interfaces between bone and cage. Relative micromotion. Posterior axial displacement. bone stress, cage stress and friction force were predicted in changes of friction coefficients, loading conditions. bone density and age-related material/geometric properties of the spinal segments. Relative micromotion (slip distance in a static loading means relative micromotion in routine activity) at the interfaces increased significantly as the mechanical properties of cancellous bone, annulus fibers or/and ligaments decrease or/and as the friction coefficient at the interfaces decreases. The contact normal force at the interfaces decreased as cancellous bone density decreases or/and as the friction coefficient increases A significant increase of slip distance at anterior annulus occurred with an addition of torsion to compressive preload. Relative micromotion decreased with an increase of disc area. In conclusion. relative micromotion, stress response. Posterior axial displacement and contact normal force are sensitive to the friction coefficient of the interfaces, bone density, loading conditions and age-related geometric/material changes.

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Study on Adhesive Strength of Polymer Modified Cement Mortar for Maintenance in Concrete Structure (콘크리트 구조물 보수용 폴리머시멘트 모르타르의 부착강도 특성에 관한 연구)

  • Park, Sang-Soon;Kim, Jung-Heum
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.5
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    • pp.128-135
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    • 2010
  • Polymer-modified cement mortar(PCM) has been widely used for strengthening of the concrete structures due to its excellent physical properties such as high strength and durability. Adhesive strength or behavior, on the other hands, between PCM and concrete is very important in strengthening the concrete member using PCM. Therefore the adhesive failure mechanism between PCM and concrete should be fully verified and understood. This study was performed to evaluate adhesive strength of PCM to the concrete by the direct pull-out test. In the direct pull-out tests, the adhesive strength under the various pre-treatment conditions such as immersion, thunder shower, freezing and thawing are evaluated. Also, the field direct pull-out test are performed to investigate the adhesive strength of mock-up test specimens. In the results of the test, the adhesive strength value by field test are lower than those of the standard curing condition. From these comparison and investigation, field test result was similar with the thunder shower test result. The results of the test was used to evaluate the korean industrial standard of polymer modified cement mortars for maintenance in concrete.

Organic-inorganic Nanocomposite Adhesive with Improved Barrier Property to Water Vapor for Backsheets of Photovoltaic Modules (태양광모듈용 저가형 백시트 제조를 위한 고수분차단성 유무기 나노복합형 접착제)

  • Hwang, Jin Pyo;Lee, Chang Hyun
    • Membrane Journal
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    • v.25 no.6
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    • pp.530-537
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    • 2015
  • Photovoltaic (PV) modules are environmentally energy conversion devices to generate electricity via photovoltaic effect of semiconductors from solar energy. One of key elements in PV modules is "Backsheet," a multilayered barrier film, which determines their lifetime and energy conversion efficiency. The representative Backsheet is composed of chemically resistant poly(vinyl fluoride) (PVF) and cheap poly(ethylene terephthalate) (PET) films used as core and skin materials, respectively. PVF film is too expensive to satisfy the market requirements to Backsheet materials with production cost as low as possible. The promising alternatives to PVF-based Backsheet are hydrocarbon Backsheets employing semi-crystalline PET films instead of PVF film. It is, however, necessary to provide improved barrier property to water vapor to the PET films, since PET films are suffering from hydrolytic decomposition. In this study, a polyurethane adhesive with reduced water vapor permeation behavior is developed via a homogeneous distribution of hydrophobic silica nanoparticles. The modified adhesive is expected to retard the hydrolysis of PET films located in the core and inner skin. To clarify the efficacy of the proposed concept, the mechanical properties and electrochemical PV performances of the Backsheet are compared with those of a Backsheet employing the polyurethane adhesive without the silica nanoparticles, after the exposure under standard temperature and humidity conditions.

Characterization of Water Absorption by CFRP Using Air-Coupled Ultrasonic Testing (공기결합 초음파탐상에 의한 CFRP 복합재의 흡습 특성 평가)

  • Lee, Joo-Min;Lee, Joo-Sung;Kim, Yong-Kwon;Park, Ik-Keun
    • Journal of the Korean Society for Nondestructive Testing
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    • v.34 no.2
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    • pp.155-164
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    • 2014
  • Carbon-fiber-reinforced plastic (CFRP) composites are increasingly being used in a variety of industry applications, such as aircraft, automobiles, and ships because of their high specific stiffness and high specific strength. Aircraft are exposed to high temperatures and high humidity for a long duration during flights. CFRP materials of the aircraft can absorb water, which could decrease the adhesion strength of these materials and cause their volumes to change with variation in internal stress. Therefore, it is necessary to estimate the characteristics of CFRP composites under actual conditions from the viewpoint of aircraft safety. In this study air-coupled ultrasonic testing (ACUT) was applied to the evaluation of water absorption properties of CFRP composites. CFRP specimens were fabricated and immersed in distilled water at $75^{\circ}C$ for 30, 60, and 120 days, after which their ultrasonic images were obtained by ACUT. The water absorption properties were determined by quantitatively analyzing the changes in ultrasonic signals. Further, shear strength was applied to the specimens to verify the changes in their mechanical properties for water absorption.

An Experimental study on the Structural Performance by the Depth Variation of Capacity of U-shaped composite Beam (U-형 복합보의 춤 변화에 따른 구조성능에 관한 실험적 연구)

  • Ha, Sang-Su
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.24 no.5
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    • pp.111-118
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    • 2020
  • The U-shaped composite beam used in this study consist of a reinforced concrete structure, a beam steel structure supporting the slab, a reinforced concrete structure, and a U-shaped steel plate. The U-shaped composite beam was developed for the purpose of using it as a parking lot because it is highly constructible and has low floor height and long span. For the improvement of constructivity, the U-shaped composite beam ends are planned with standardized H-shaped steel and connected directly to the columns, and the middle of the U-shaped composite beam consists of U-shaped steel plates folded in U-shaped form using thin steel plates (t=6) instead of H-shaped steel. In the middle of the composite beam, where U-shaped steel plates are located, the depth of U-shaped beam may be planned to be small so as to satisfy the height limit of the parking lot. It is important to grasp the structural performance according to the change of depth because low beam depth is advantageous for the reduction of the floor height, but it is a inhibitor to the structural behaviors of U-shaped composite beam. In addition, since U-shaped composite beams are a mixture of steel frame structures, reinforced concrete structures and U-shaped steel plates, securing unity has a great influence on securing structural performance. Therefore, in this study, a structural experiment was conducted to understand the structural performance according to the depth change for U-shaped composite beam. A total of three specimens were planned, including two specimens that changed the depth using a criteria specimen planned for a general parking lot. The results of the experiment showed that the specimens who planned the depth greatly had better structural performance such as yield strength, maximum strength, and energy than the standard specimen.

Comparative Analysis of Pre-processing Method for Standardization of Multi-spectral Drone Images (다중분광 드론영상의 표준화를 위한 전처리 기법 비교·분석)

  • Ahn, Ho-Yong;Ryu, Jae-Hyun;Na, Sang-il;Lee, Byung-mo;Kim, Min-ji;Lee, Kyung-do
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1219-1230
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    • 2022
  • Multi-spectral drones in agricultural observation require quantitative and reliable data based on physical quantities such as radiance or reflectance in crop yield analysis. In the case of remote sensing data for crop monitoring, images taken in the same area over time-series are required. In particular, biophysical data such as leaf area index or chlorophyll are analyzed through time-series data under the same reference, it can be directly analyzed. So, comparable reflectance data are required. Orthoimagery using drone images, the entire image pixel values are distorted or there is a difference in pixel values at the junction boundary, which limits accurate physical quantity estimation. In this study, reflectance and vegetation index based on drone images were calculated according to the correction method of drone images for time-series crop monitoring. comparing the drone reflectance and ground measured data for spectral characteristics analysis.

Bond Characteristics and Splitting Bond Stress on Steel Fiber Reinforced Reactive Powder Concrete (강섬유로 보강된 반응성 분체 콘크리트의 부착특성과 쪼갬인장강도)

  • Choi, Hyun-Ki;Bae, Baek-Il;Choi, Chang-Sik
    • Journal of the Korea Concrete Institute
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    • v.26 no.5
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    • pp.651-660
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    • 2014
  • Structural members using ultra high strength concrete which usually used with steel fiber is designed with guidelines based on several investigation of SF-RPC(steel fiber reinforced reactive powder concrete). However, there are not clear design method yet. Especially, SF-RPC member should be casted with steam(90 degree delicious) and members with SF-RPC usually used with precast members. Although the most important design parameter is development method between SF-RPC and steel reinforcement(rebar), there are no clear design method in the SF-RPC member design guidelines. There are many controversial problems on safety and economy. Therefore, in order to make design more optimum safe design, in this study, we investigated bond stress between steel rebar and SF-RPC according to test. Test results were compared with previously suggested analysis method. Test was carried out with direct pull out test using variables of compressive strength of concrete, concrete cover and inclusion ratio of steel fiber. According to test results, bond stress between steel rebar and SF-RPC increased with increase of compressive strength of concrete and concrete cover. Increasing rate of bond stress were decrease with increase of compressive strength of SF-RPC and concrete cover significantly. 1% volume fraction inclusion of steel fiber increase the bond stress between steel rebar and SF-RPC with two times but 2% volume fraction cannot affect the bond stress significantly. There are no exact or empirical equations for evaluation of SF-RPC bond stress. In order to make safe bond design of SF-RPC precast members, previously suggested analysis method for bond stress by Tepfers were evaluated. This method have shown good agreement with test results, especially for steel fiber reinforced RPC.