• Korean Journal of Food Science and Technology
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• v.29 no.4
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• pp.671-679
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• 1997

Approximate composition and mineral content of eighty seven medicinal herbs marketed in Korea was analyzed on the basis of their dried weight. From the results anlyzed, it was known that Acanthopanax sessiliflorum Seem. (22.4%), Portulaca oleracea L. (20.6%), Torilis japonica DC. (15.0%) in crude ash, Ziziyphus spinosa Hu. (39.9%), Alisma orientale Juzep. (30.6%), Lepidium latifolium L. (28.8%), Cassia tora L. (27.5%) in crude protein, Ziziyphus spinosa Hu. (27.9%), Xantnium strumarium L. (24.0%), Perilla frutescens var. acuta Kude (22.8%), Benincasa hispida Cong. (22.4%) in crude lipid, Vitex rotundifolia L. (59.5%), Ganoderma lucidum Karst (53.9%), Pinus densifiora Sieb. et Zucc. (46.4%), Sambucus williamsii Hanse (41.2%) in crude fiber, and Poria cocos Wolf (91.2%), Gastrodiae elata Blume (87.9%), Gentiana scabra Bunge (86.3%), Rehnannia glutinosa Libosch (86.0%) in nitrogen free extract exhibited higher content than other kinds. Phosphrous contents were 1.5, 1.1 and 1.0% in order of Taxillus chinensis Danser, Malva verticillata L., Nelumbo nucifera Gaeten, and magnesium contents were 0.7, 0.5 and 0.5% in the order of Saururus chinesis Baill., Salivia milltriorrhiza Bunge and Pueraria thunberglans Benth. Calcium contents of Cimicifuge heradeifolia Komarov, Acanthopanax sessiliflorum Seem. and Rhus vericiflura Stokes were 8.5, 7.1 and 3.0%, and potassium contents of Tricholoma matsutake Sing., Houttuynia cordata Thunb., Achyranthes japonica Nakai were 5.6, 5,4 and 4.7%, respectively. Sodium contents were 2.1, 0.6 and 0.3% in the order of Boshinakia rossica Fedtsch., Eucommia ulmoides Oliv. and Prunus mume Seib., and iron contents were 0.3, 0.2 and 0.2% in the order of Cirsium japonicum var, ussuriense Kitamuraa, Gentiana scabra Bunge and Phlomis umbrosa Turcz.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.467-474
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• 2010

Cheonggukjang is one of the traditional fermented soy-based foods in Korean diets. Studies in cell cultures, humans have revealed anti-hypertension, anti-stress, anticancer, antioxidant, immune enhancing effects. Angelica gigas, Rehmanniae radix, and Red ginseng are popular medicinal plants and widely used for oriental medicine. In this study a strategy had been developed to mobilize beneficial phenolics from Angelica gigas, Rehmanniae radix, and Red ginseng combined with fermented soy by Cheonggukjang fermentation for antioxidant and Type II diabetes management. The quality and functional characteristics of Chenggukjang fermented with Angelica gigas, Rehmanniae radix and Red ginseng. Cheonggukjang (CKJ), Angelica gigas Cheonggukjang (CKJ-DD), Rehmanniae radix Cheonggukjang (CKJ-RG), Angelica gigas and Rehmanniae radix Cheonggukjang (CKJ-DD+RG) and Red ginseng Cheonggukjang (CKJ-RED) were evaluated. The mobilized phenolic profile was evaluated for antioxidant activity and the potential to inhibit ${\alpha}$-amylase linked to hyperglycaemia. This research has important implications for the development of functional soy-based-fermented foods enriched with Angelica gigas, Rehmanniae radix and Red ginseng phenolics for oxidative stress - induced diabetic complications. Furthermore, Hunter's color values of 5 types cheonggukjang, lightness (L-values), redness (a-values) and yellowness (b-values) were evaluated. Free amino acid content of CKJ-RED (0.993 mg/gd. w.) showed higher than that of CKJ (0.205 mg/g-d.w.).

• Korean Journal of Medicinal Crop Science
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• v.8 no.4
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• pp.351-361
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• 2000

The juice extract of Rehmannia glutinosa Liboschitz was used to improve the productivity of ethanol in alcohol fermentation process using a 5 L fermentor under batch and fed-batch cultivations. For batch cultivation, both cell density and ethanol production were increased as the extract of R. glutinosa was increased, showing 11.8 (g/L) of maximum cell density and 0.092 (% /hr) of maximum alcohol productivity in adding 30% (v/v) of the extract. However, in adding more than 40% of the extract both cell growth and ethanol production were dropped. The cell growth was severely inhibited in 50% addition. It was found that fed-batch cultivation in adding 30% of the extract of R. glutinosa was an effective process than batch cultivation, yielding up to 30% cell growth and ethanol production. This ethanol productivity was also 30-40% higher than that obtained from a conventional alcohol fermentation. It can tell that dried R. glutinosa Liboschitz is to be used for both enhancing the yield of alcohol fermentation and utilizing biologically active substances possibly transported from R. glutinosa Liboschitz into fermented broth.

• The Korean Journal of Pesticide Science
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• v.4 no.3
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• pp.60-67
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• 2000

This study was conducted to elucidate extraction efficiency by microwave in comparison with Soxhlet for extraction of pesticide residues in dried medical herbs; red-ginseng, white-ginseng, Bupleuri Radix, Angelica gigas Nakai, Rehmannia glutinosa. The acetone extraction by microwave of tolclofos-methyl and quintozene in medical herbs was efficient. The extraction efficiency by microwave with power 45 to 150 watts, extraction time 1 to 5 minutes and solvent volume 30 ml was compared with that of Soxhlet with extraction time 7 hours and solvent volume 150 ml. The extraction efficiency by microwave with extraction time 3 to 5 minutes was similar with extraction time of 7 hours by Soxhlet. When medical herbs spiked with tolclofos-methyl and quintozene was analyzed to how the extraction efficiency of microwave by kind of medical herbs, the extraction efficiency by microwave with extraction time of 3 to 5 minutes was the same as Soxhlet extraction. The optimal condition for extraction of tolclofos-methyl and quintozene in medical herbs by microwave was 45 to 90 watts of power supply, 3 to 5 minutes of extraction time and acetone 30 ml of solvent volume.

• Korean Journal of Medicinal Crop Science
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• v.27 no.5
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• pp.315-321
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• 2019

Background: There have been no studies to date on rhizome development and optimal harvest timing for Rehmannia glutinosa. We therefore, undertook this investigation. Methods and Results: R. glutinosa 'Jihwang 1' was sown in early May and harvested in early November. Growth investigations were carried out at intervals of 10 days between 90 and 180 days after sowing (DAS). Leaf length, leaf width, and number of leaves increased until 150 DAS but decreased after 160 DAS. Rhizome length increased until 120 DAS subsequently, rhizome diameter increased rapidly between 130 and 150 DAS. Thus, the key period for rhizome enlargement in R. glutinosa is thought to be 130 to 150 DAS. Fresh root yield increased sharply from 916 kg/10a to 1,914 kg/10a between 4 and 5 months after sowing (MAS). Dry matter ratio increased gradually from 19.2% at 4 MAS to 24.4% at 6 MAS. Finally, the level of catalpol, a key active ingredient, increased sharply from 0.41% to 4.21% between 5 and 6 MAS. Given the dry matter ratio, catalpol content and yield measured, we suggest that optimal R. glutinosa harvest time is 6 MAS. Conclusions: Based on our results, the key period for rhizome enlargement is 130 to 150 DAS and optimal harvest timing is 6 MAS. We anticipate that these and other results of this study can be used to inform cultivation of R. glutinosa.

• Korean Journal of Medicinal Crop Science
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• v.27 no.3
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• pp.202-207
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• 2019

Background: Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae and is used in traditional oriental medicine. This study was conducted to evaluate the appropriateness of plug-seedling cultivation for replanting of R. glutinosa. Methods and Results: The seed rhizomes of Rehmannia glutinosa 'Jihwang 1' were harvested in the middle of March, 2018. Direct seedling cultivation was conducted after air curing for 3 days. The seedlings for transplanting were cultivated for 30 (30D), 45 (45D), and 60 days (60D). The mat formation rates of the 30D, 45D and 60D treatment seedling were 81.3%, 100%, and 100%, respectively. Of the tree treatment groups, flowering occurred only in the 45D and 60D treatment groups, and their flowering rates were $7.3{\pm}3.06%$ and $13.3{\pm}1.15%$, respectively. The agronomic characteristics and active components of the three treatment groups were evaluated in comparison with the DS1 (directly seeded group, control) and DS2 (directly seeded group, replanting) groups. As a result, the leaf length and leaf width of the 30D seedlings were higher than other treatment groups, but the number of leaves per plant was statistically the same. In addition, root diameter and yield of the 30D seedling were statistically the same as DS1. Moreover the catalpol contents of 30D, 45D, DS1 and DS2 group seedlings were 2.23%, 1.86%, 2.50% and 2.08%, respectively. Conclusions: Taken together, these findings suggest it is feasible to use the plug-seedling cultivation method for replanting of R. glutinosa. seedlings. However, further evaluation of the economic aspect is needed.

• Korean Journal of Medicinal Crop Science
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• v.27 no.4
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• pp.271-277
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• 2019

Background: Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. has long been used as a traditional medicinal plant in Korea. This study was carried out to investigate the emergence conditions during the seedling periods in R. glutinosa. Methods and Results: The rhizomes of R. glutinosa variety (Jihwang 1) were harvested in the 22, March, 2018. The rhizomes were sown on in 50-cell plug trays. The emergence rates of seedlings at $15^{\circ}C$, $20^{\circ}C$, $25-40^{\circ}C$, and $45^{\circ}C$ treatment seedling were 1.3%, 96%, 100% and 0%, respectively. Rhizome rot was occurred at the temperature of $15^{\circ}C$ and $45^{\circ}C$. The emergence rates of seedlings in high moisture (HM), moderate moisture (MM) and low moisture (LM) treatments at $35^{\circ}C$ were 99.3%, 100%, and 0%, respectively. Drought damage was recorded in plant with the LM treatment. Seedling quality surveys were carried out at 10-days intervals from 10 to 60 days after sowing (DAS). Leaf length and leaf width were increasing until 50 DAS and the number of leaves was increasing until 60 DAS. Root length was increasing until 40 DAS, and then, flowering occurred from 30 to 60 DAS. Lastly, at 40 DAS, leaf aging and root enlargement was observed. Conclusions: We concluded that the emergence of seedlings was possible in the range of 20 to $40^{\circ}C$. Considering drying and rotting damage, we concluded that the moderate level of moisture is most appropriate for seedling emergence. In addition, we concluded that optimal seedling periods are between 30 and 40 DAS.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.491-501
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• 2021

This study was carried out to investigate the occurrence of seedlings of Korean fir (Abies koreana) in Korean fir forest located on the eastern slope of Mt. Halla National Park. Between June and August in 2016, the systematic sampling method was applied to 125 plots of 5 m × 5 m quadrats and field survey was came out to measure the characteristics of sites and understory vegetation. Correlation analysis was applied to the variables quantified from the data collected. Total number of flora found at the study site is 31 in families, 44 in genera, and 56 in species. The main lower layer vegetation was investigated such as Jeju dwarf bamboo, Toothed club moss (Lycopodium serratum), Pacific fir moss (Lycopodium chinense), Climbing hydrangea (Hydrangea petiolaris) etc. Among these plant species, the vegetation which had a high positive correlation with the frequency and the coverage of seedlings of Korean fir were Jeju dwarf bamboo, Toothed club moss and Pacific fir moss.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.321-328
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• 2007

Two pig trials were conducted to test Herb MixⓇ, a mixture of Rehmannia glutinosa, Angelica gigas, Discorea japonica, Glycyrrhiza uralensis, Schisandra chinensis and Ligusticum jeholense, as a herbal additive to weaning pig diet. Exp. 1 was conducted with 45 three-way cross-bred(Y×L×D) weaning pigs randomly allocated to 3 treatments; control, Herb MixⓇ 0.15% and Herb MixⓇ Gold(Plellidendron amurense fortified Herb MixⓇ) 0.15%. Exp. 2 was conducted with 48 weaning pigs randomly allocated to 4 treatments; control, 0.1%, 0.2% and 0.3% Herb MixⓇ. There was a significant(p=0.05) difference between the control and herbal additive groups, however, no significant difference was found between Herb MixⓇ and Herb MixⓇ Gold in growth performance of Exp. 1. In Ex. 2, supplementation of Herb MixⓇ at all level(0.1%, 0.2% and 0.3%) significantly(P<0.05) improved average daily gain and feed intake, however, there were no significant differences among supplemented groups. Among the blood parameters, serum IgG level and WBC numbers were significantly lowered by Herb Mix supplementation in both experiments. Stress indicator(SI) was significantly lower in herbal additive groups in Exp. 1. Nutrient digestibility of DM and NFE in supplemented groups was lower than the control in Exp. 1. Howener, it was not significantly different among treatments in Exp. 2. Number(cfu) of fecal E.coli decreased while that of Lactobacilli increased in treated groups. It was concluded that fortifying Herb MixⓇ with Plellidendron amurense was not effective in improving the efficacy of Herb MixⓇ and supplementation of Herb MixⓇ at 0.1~0.2% level improves growth performance of weaning pigs. Blood parameters especially immunity related ones(IgG, WBC and SI) were significantly influenced.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.212-217
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• 1996

This study was carried out to select proper intercrops in peony cultivation because peo­ny is damaged easily by disease and cannot make proper income with it's single crop system, so it needs rational intercropping system to raise it's productivity and income. The results of this study are as follows. The kinds of soil nematodes were Meloidogyne sp, Pratyienchus sp, Aphelenchoides sp, Ditylenchus sp, Xiphinema sp, and damaging dominant nematode was Meloidogyne sp. The density of Meloidogyne sp wa suppressed remarkably in the combination of peony+sesame, and peony + job's tears. This study shows that sesame and job's tear were most resistant crops to the IvIeloi­dogyne sp. The quanity of herbaceous peony was the best in the combination of peony+red pepper. The results of income analysis per lOa for three years shows next conclusion. Peony+red pepper combination and peony+ sesame combination were most effective when it's compared with single cropping of peony (1,490 thousand won). But red pepper was damaged easily by soil nematode, therefore, sesame was the most stable and high yield crop as a intercrop in the cultivation of herbaceous peony.