• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.8
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• pp.1157-1164
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• 2015

The present investigation evaluated the antioxidant activities of water extracts from dandelion (Taraxacum officinale) aerial parts, roots, and mixed extracts. Mixed extract of T. officinale was a mixture of aerial parts and roots at 9:1 and 8:2 weight ratios. Extracts from aerial parts (DAE), roots (DRE), and mixture of aerial parts and roots (DME) were measured for cell viability and catalase activity in HepG2 cells, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and lipid peroxidation inhibitory activity. Cell viabilities of HepG2 cells treated with DAE, DRE, DME 8:2, and DME 9:1 against $H_2O_2$-induced oxidative damage were 63.4%, 54.6%, 76.7% and 83.4% at a concentration of $400{\mu}g/mL$, respectively. Catalase activity was highest in DME 9:1 (12.2 mU/min/mg protein) compared with DAE (9.0 mU/min/mg protein) and DRE (9.7 mU/min/mg protein). DPPH radical scavenging activity of DME showed a significantly lower $EC_{50}$ value than DAE ($EC_{50}$ value of DME $9:1=163.3{\mu}g/mL$, DME $8:2=172.4{\mu}g/mL$, and $DAE=173.7{\mu}g/mL$). Lipid peroxidation inhibitory activity of DME showed a significantly lower $EC_{50}$ value than DAE [$EC_{50}$ values of DME $(9:1)=454.4{\mu}g/mL$, DME $(8:2)=426.6{\mu}g/mL$, and $DAE=654.7{\mu}g/mL$]. The results indicate that a small amount of T. officinale roots increased antioxidant activity of aerial parts. Especially, a 9:1 mixture was more valuable than 8:2 mixture for industry.

• Journal of Korean Society of Forest Science
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• v.100 no.1
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• pp.42-51
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• 2011

This study was conducted to analyze correlation of above- and below-ground biomass and to drive regression equation suitable for estimating standing tree biomass between natural and planted stands of Pinus densiflora for. erecta of one age-class in Gangwon province, Republic of Korea. Total 40 trees, 10 from the naturally regenerated and 10 from the planted stands in each of two studied sites, were uprooted to measure height, diameter at root color (DRC), and the dry weights of stem, branches, and needles. The length, weight, and volume of the main and horizontal roots were also measured. Most of the above-ground traits except height were highly correlated with most of the other above-ground traits and the below-ground traits except the length of roots (p < 0.05). Especially, the DRC, which is measured easily on the standing tree, was highly correlated with most of the traits in all studied stands (p < 0.01). Thus, the DRC would be the most desirable trait to estimate not only above-ground biomass but also below-ground biomass. However, height was not a good variable to estimate standing tree biomass of Pinus densiflora for. erecta of one age-class in Gangwon province because it was not correlated with most of other traits. Regression equations derived from the current study could be used effectively as a basic data for estimating above-ground and below-ground biomass using DRC.

• Ecology and Resilient Infrastructure
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• v.9 no.4
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• pp.259-268
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• 2022

In this study, seeds of Festuca ovina var. coreana growing in waste coal landfills exposed to heavy metal contamination for a long time were collected, and growth characteristics and heavy metal accumulation capacity were evaluated through greenhouse cultivation experiments with germinated seedlings, and was conducted for the applicability of phytoremediation technology. Concentration gradients of arsenic-treated artificial soil were 25, 62.5, 125, and 250 mg/kg, respectively, lead concentrations were 200, 500, 1000, and 2000 mg/kg, and cadmium concentrations were 15, 30, 60, and 100 mg/kg, respectively In the arsenic, lead, and cadmium-treated experimental groups, the number of leaves of F. ovina var. coreana decreased in all compared to the control group except for the lead-treated groups (200, 500, and 1000 mg/kg). Length growth of the shoot part was increased in all of the arsenic treatment groups compared to the control group, but decreased in all of the root parts. In the 1000 and 2000 mg/kg lead treatment groups, lengths increased compared to the control group, but in the other treatments, they were shorter than the control group. In the case of the cadmium treatment group, all of the shoot parts were increased compared to the control group, and all of the root parts were decreased. In the case of arsenic treatment, the biomass was decreased at all parts and all concentrations compared to the control group. The 200, 500, and 1000 mg/kg lead treatments showed larger biomass than the control group in both shoot and root parts. In the cadmium treatment group, the biomass of both shoot and root parts decreased compared to the control group. As the concentration of heavy metal treatment increased, both the number of leaves and the biomass by plant parts tended to decrease, and the length growth of the shoot part tended to increase slightly, but the root part tended to decrease slightly. The arsenic accumulation concentrations of the shoot and root parts of the 62.5 mg/kg arsenic treatment area were 9.4 mg/kg and 253.3 mg/kg, respectively. While the shoot part of the 250 mg/kg arsenic treatment area withered away, the arsenic accumulation concentration in the root part was analyzed to be 859.1 mg/kg, In the 2,000 mg/kg lead treatment area, the shoot and root parts accumulated 10,308.1 and 11,012.0 mg/kg, which were 1.1 times higher than the root parts. At 100 mg/kg cadmium treatment, the shoot and root parts were 176.0 and 287.2 mg/kg, and the root part accumulated 1.6 times higher than the shoot part. As a result of tolerance evaluation of F. ovina var. coreana, multi-tolerance to three heavy metals was confirmed by maintaining growth without dying in all treatment groups of arsenic, lead, and cadmium. Plant extraction (phytoextraction) of F. ovina var. coreana was verified as a species that can be applied up to 2,000 mg/kg of soil lead contamination.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.278-282
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• 2004

We investigated antimicrobial activity of methanol extract from Angelica gigas and A. acutiloba against three gram-positive bacteria(Bacilius cereus, B. subtilis, and Staphylococcus aureus), two gram-negative bacteria (Escherichia coli and Pseudomonas fluorescens), and one yeast (Saccharomyces cerevisiae). The antimicrobial activity was shown in only ethyl acetate fraction of shoot and root extracts from A. gigas. The activity of the fraction of shoot extract was remarkably potent against S. aureus. The ethyl acetate fraction of root extract showed great antimicrobial activity against B. subtilis and E. coli. The n-hexane fraction of shoot extract and the water fraction of root extract from A. acutiloba were not showed antimicrobial activity, in contrast, the ether fraction of shoot and root extracts showed great antimicrobial activity. S. cerevisiae was not inhibited by any fractions and species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.3
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• pp.294-300
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• 1985

This experiment was carried out to study the root and the other major agronomic traits of barley cultivars at the greenhouse and natural conditions for the accelerating of breeding generation. The barley cultivars tested in this experiment were Kangbori, Olbori and Suwon 18. In early stages, total dry weight of barley was vigorously increased at the greenhouse condition, whereas it was less increased than that of natural condition after heading stages. In dry root weight, it was decreased at natural condition rather than greenhouse condition. The appearance of harvest dry root weight was at four weeks after heading at natural condition and seven weeks after heading at greenhouse condition, respectively. It showed different tendency in T/R ratio, indicating the continuous increased at natural and greenhouse condition, even if there is more or less decrease until tillering stage at greenhouse condition.

• Journal of Bio-Environment Control
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• v.32 no.3
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• pp.205-209
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• 2023

Mechanical stimulation induce the morphological changes in plants. In this study, we investigated the growth changes of tomato seedlings applicated to mechanical stimulation. The brushing treatment was used for mechanical stimulation. The brushing treatment interval was 2 hr using transfer device attached acrylic film from 10 days after sowing. Growth parameter of tomato seedlings were measured 3-day intervals to investigate the growth changes during brushing treatment. The plant height and leaf area were decreased in brushing treatment than the control, and the fresh and dry weights of shoot didn't have significant difference in the control and brushing treatment. The total root length and root surface area were increased in brushing treatment compared than the control, and root volume has no significant difference in the control and brushing treatment. In conclusion, these results suggest that the application of brushing treatment on tomato seedling make shorten plant height and well-development root morphological characteristics.

• Journal of Plant Biology
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• v.6 no.3
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• pp.6-14
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• 1963

YIM, Kyong Bin (Coll. of Agriulture, Seoul National University) Effect of neutron and gamma irradiation on the germination of diploid and tetraploid rye seeds. Kor. Jour. Bot. VI(3):6-14, 1964. Tetraploid rye, Secale cereale 4x, was more tolerant to fast nuetron than diploid rye. Root growth was more suppressed than was seedling height in both diploid and tetraploid rye. A stimmulative effect on the dry weight of the shoot could be observed at very low doses of irradiation. It was the fact that the lower the moisture content of the seeds, the higher the radiosensitivity. Concerning seedling height growth, the effectiveness ratio of N/X equalled about 20.0 in diploid rye and about 18.2 in tettraploid rye, when the 50% dose ratios is used for this quotient calculaiton.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.20-24
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• 2019

Moringa oleifera Lam (MOL) has been used as a traditional medicine to treat various cancers and inflammation. Whereas the bioactivities of the MOL leaf and seed are well reported, the study of the root is still rudimentary. In this study, the ethanol extract of MOL (EEMOL) and its subsequent organic solvent fractions were prepared and their anticoagulation activity in vitro and platelet aggregation inhibitory activity were evaluated. The EEMOL had negligible anticoagulation and strong platelet aggregation activities. However, the hexane and ethyl acetate fractions of EEMOL showed significant inhibition against thrombin, prothrombin, coagulation factors, and platelet aggregation, without hemolytic activity up to 1.0 mg/ml. Our results suggest that the active fractions of MOL root have potential as new anti-thrombosis agents.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.30-30
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• 2022

최근 기후변화로 인한 국지성 호우 빈도 및 강수량이 급증하는 등 극한강우 발생 가능성이 높아지고 있는 실정이다. 공공 기반의 유역 및 지자체별 침수 대응은 지속적으로 이루어지고 있으나, 건설 현장 대응은 이에 비해 미흡한 실정이다. 특히, 건설 현장의 경우, 예측할 수 없는 홍수 유출에 대해서도 기존 설계시 반영된 홍수 유출량과 기상청 정보에만 의존하고 있어 극한강우 발생시 취약성을 나타낼 수 있다. 특히, Top-down 현장은 개구부, 표면 작업을 위한 포장 등에 의해 지하부로 유입되는 강우량이 많고, 지하 굴착공사시 단차 및 지하수 발생으로 극한강우시 침수에 의한 수재해 발생 확률이 높다. 이를 대비하기 위해 XP-SWMM 모형을 이용하여 지상부와 지하부의 강우-유출량을 산정하고 지하부 침수를 모의하였다. 실제 Top-down 현장조사를 통해 침수 관련 인자와 XP-SWMM을 연계하여 침수모의 기법에 적용하였다. 관련 주요인자는 강우량, 현장 지상부 면적, 지상부 배수로, 지하 유입부, 지하 배수펌프 등으로 현장 조사결과 나타났다. 강우자료의 경우, 극한강우를 고려하기 위해 현장 지역의 최대 강우량, 태풍 루사와 기상청 강우의 증가 시나리오를 고려하여 모의에 적용하였다. 본 연구에서는 극한강우에 대한 Top-down 침수 모의를 수행할 수 있는 상용 모델링과 이와연관된 인자를 도출하여 침수 모의 기법을 최적화 하였다. 이러한 침수 모의를 통해 Top-Down 현장 침수심 등을 예측할 수 있다. 향후 이를 통해 지하공간이 있는 건설현장의 강우-유출 현상및 침수 모의가 가능하고, 실시간 현장별 침수 예측 모델 개발로 현장별 대피경로 및 대응방안을 제시하여 인적 피해를 최소화할 수 있을 것으로 기대할 수 있다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.80-87
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• 2011

Panax ginseng has been used as a traditional medicine for several centuries in Korea. A laboratory experiment using methanol extracts of freeze-dried leaves and roots in the different ages of P. ginseng was conducted to determine the content of phenolics and flavonoids, antioxidant activity and cytotoxicity. The results indicate that the total phenolics level [mg ferulic acid equivalents (FAE) $kg^{-1}$ DW] was higher in leaves (22.0 to 76.3 mg $kg^{-1}$) than roots (19.0 to 28.3 mg $kg^{-1}$) of P. ginseng. The total content of phenolics in roots increased with increase in age of P. ginseng from one to six years. However, the content of phenolics in P. ginseng leaf decreased with the increase in age. Total flavonoid [mg naringin equivalents $kg^{-1}$ DW] was more detected in the leaves (30.3 to 138.6 mg $kg^{-1}$) than in the roots (0.0 to 10.6 mg $kg^{-1}$) of P. ginseng. The total flavonoid level in leaves decreased with increase in age of P. ginseng. The antioxidant potential of the methanol extracts from the plants dose-dependently increased. DPPH free radical scavenging activity was higher in leaves (36.9 to 82.8%) than in roots (14.8 to 39.4%), and in young plants than in old ones. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the methanol extracts from 5 year-root part showed the highest cytotoxicity against Calu-6, followed by 2 year- and 3 year-roots. However, the methanol extracts from 6 year- and 4 year-roots had lower cytotoxicity. Total phenolics content in both leaves and roots was highly correlated with the DPPH radical scavenging ($r^2=0.7366$ to 0.7870) and nitrite scavenging ($r^2=0.5604$ to 0.8794) activities, suggesting that they contribute to the antioxidant properties of the P. ginseng plants.