• Title/Summary/Keyword: 지연선

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Purificaton and Some Properties of Polyphenol Oxidase from Ginko biloba Leaves (은행잎에서 분리한 Polyphenol Oxidase의 정제 및 특성)

  • Seol, Ji-Yeon;Park, Soo-Sun;Kim, An-Keun
    • Korean Journal of Pharmacognosy
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    • v.30 no.3
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    • pp.306-313
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    • 1999
  • Polyphenol Oxidase(PPO) was purified from an extract of Ginkgo biloba leaves by ammonium sulfate fractionation followed by sephadex G-150 column chromatography, which resulted in a 18-fold increase in specific activity. The enzyme was most active at pH 8.5 and the temperature optimum for the PPO catechol oxidation reaction was $45^{\circ}C$. Heat inactivation studies showed that heating for 7, 9 and 48 min, at 80, 70 and $60^{\circ}C$ respectively caused a 50% loss in enzymatic activity and that the enzyme was completely inactivated after heat treatment at $90^{\circ}C$ for 60 min. Km values of the PPO for catechol, hydroquinone and 4-methylcatechol derived from Lineweaver-Burk plots were $6.06\;{\times}\;10^{-4}M,\;1.02\;{\times}\;10^{-3}M,\;1.41\;{\times}\;10^{-3}M$ respectively. Of the substrates tested, 4-methylcatechol was oxidized most readily and the enzyme did not oxidize monophenols. The enzyme datalyzed browning reaction was completely inhibited in the presence of reducing reagents, namely ascorbic acid, cysteine, glutathione, 2-mercaptoethanol, potassium metabisulfite at 0.5 mM level. Sodium chloride showed very little inhibition effect on Ginkgo biloba leaves PPO. Lineweaver-Burk analysis of inhibition data revealed that the inhibition by cysteine, 2-mercaptoethanol, potassium cyanide was competitive with ki values of $1.1\;{\times}\;10^{-5}M,\;2.4\;{\times}\;10^{-5}M,\;8\;{\times}\;10^{-5}M$, respectively. Among the divalent cations, $Cu^{2+}ion$ was a strong activator on PPO and $Mn^{2+}ion$ was little or no effect on PPO activity $Ni^{2+}ion$ was an inhibitor on PPO.

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A survey on hygiene management for raw by-products of beef in Gwangju area, Korea (광주지역 생식용 소 부산물의 위생관리 실태 조사)

  • Kim, Ji-Yeon;Jang, Mi-Sun;Koh, Ba-Ra-Da;Ji, Tea-Kyung;Sung, Chang-Min;Park, Da-Hae;Kim, Hyun-Joong;Kim, Eun-Sun;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
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    • v.36 no.3
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    • pp.209-216
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    • 2013
  • A total of 301 samples of bovine liver, spleen and omasum were collected from butchers and restaurants in Gwangju, Korea during 2012 and all samples were subjected to bacteriological examination and antibiotic residues. Also, this study was performed to survey the consciousness for hygiene of livestock workers who are handling bovine by-products in Gwangju. The detection rate of aerobic plate count (APC) was higher in summer than in other seasons in all by-products (P=0.000). The detection rate of E. coli count was lower in the liver than the spleen and omasum (P=0.000). Twenty four of the samples (8.0%) were contaminated with S. aureus while one spleen sample (0.3%) was contaminated with L. monocytogenes and finally 10 (3.3%) of the liver and omasum samples were contaminated with Cl. perfringens. Five of the twenty-four S. aureus isolates harbored enterotoxin gene. However, the cpe gene of Cl. perfingens was not detected among any of the 10 isolates. Antibiotic residues were not detected in the liver samples. The consciousness survey's results showed that most of them (58.8%) were safe.

MAC Address Spoofing Attack Detection and Prevention Mechanism with Access Point based IEEE 802.11 Wireless Network (Access Point 기반 무선 네트워크 환경에서의 MAC Address Spoofing 공격 탐지 및 차단 기법)

  • Jo, Je-Gyeong;Lee, Hyung-Woo
    • Journal of Internet Computing and Services
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    • v.9 no.4
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    • pp.85-96
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    • 2008
  • An authentication procedure on wired and wireless network will be done based on the registration and management process storing both the user's IP address and client device's MAC address information. However, existent MAC address registration/administration mechanisms were weak in MAC Spoofing attack as the attacker can change his/her own MAC address to client's MAC address. Therefore, an advanced mechanism should be proposed to protect the MAC address spoofing attack. But, existing techniques sequentially compare a sequence number on packet with previous one to distinguish the alteration and modification of MAC address. However, they are not sufficient to actively detect and protect the wireless MAC spoofing attack. In this paper, both AirSensor and AP are used in wireless network for collecting the MAC address on wireless packets. And then proposed module is used for detecting and protecting MAC spoofing attack in real time based on MAC Address Lookup table. The proposed mechanism provides enhanced detection/protection performance and it also provides a real time correspondence mechanism on wireless MAC spoofing attack with minimum delay.

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An Interpretative Phenomenological Analysis on University Members' Information Needs and Behaviors (대학구성원의 정보요구 및 행위에 대한 해석현상학적 연구)

  • Min, Yoonkyung;Kim, Giyeong;Lee, Jee Yeon;Kim, Hyeyoung;Seo, Jung Sun;Lee, Go Eun;Lee, Na Ri;Jung, Minji;Chae, Hyun Soo
    • Journal of the Korean Society for Library and Information Science
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    • v.54 no.2
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    • pp.269-297
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    • 2020
  • This study aims to understand the information needs and behaviors of university members given the societal shifts incited by the fourth industrial revolution as well as the changes in university research and education. We first conducted several in-depth interviews with diverse university members, and then adopted interpretative phenomenological analysis(IPA) to search the university members' information needs and behaviors including the contexts for such needs. The interviewees demonstrated more complex and convergent information needs than traditional information needs. They had very individualized information needs according their particular situations and took integrated problem-solving methods through their personal networks and/or making their own communities. When the interviewees' information needs were not satisfied by libraries, they tended to show low awareness of the libraries.

Preparation and Hypoglycemic Effect of Reconstituted Grain Added with Selected Medicinal Herb Extract (한방 생약재 추출물을 첨가한 재성형 당뇨쌀의 제조 및 혈당강하 효과)

  • Lee, Young-Tack
    • Korean Journal of Food Science and Technology
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    • v.35 no.3
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    • pp.527-531
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    • 2003
  • Hot-water extract from selected medicinal herbs of diabetes prescription was mixed with brown rice and pearled barley flours and extruded to prepare a reconstituted grain for diabetes millitus patients. Even though the cooked reconstituted grain containing medicinal herb extract was somewhat different from cooked ordinary rice in textural properties measured by texture analyzer, it was estimated to be organoleptically acceptable in sensory parameters. The reconstituted grain added with medicinal herb extract reduced the rate of dialysis of glucose evaluated by in vitro dialysis experiment. The effects of reconstituted grain diet on blood glucose levels in diabetes patients were studied during 3-week period. Compared to normal diet, the reconstituted grain diet for 2 weeks significantly decreased the fasting and 2-hr postprandial blood glucose levels in diabetes patients by 14% and 10%, respectively. These results suggested that the reconstituted grain could be used as an effective therapeutic diet for the control of diabetes mellitus.

Design of Ultra-Wide Band Antenna with Compact MCR Structure for BW Enhancement (대역폭 향상을 위한 소형 MCR 구조를 갖는 Ultra-Wide Band 안테나 설계)

  • Nadeem, Iram;Kim, Young-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.42 no.4
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    • pp.798-805
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    • 2017
  • This paper presents a low profile multi circular ring with different radius, patch antenna with modified feed line and slotted ground. The size of the antenna is $15{\times}12mm^2$, having electrical dimensions of $0.14{\lambda}{\times}0.12{\lambda}$ (at lower initial frequency) and footprints of $180mm^2$. The proposed antenna covers 3.1 to 12.3 GHz, reflection coefficient up to -38 dB with Bandwidth ratio of 4.13:1 and fractional Bandwidth of 122%. Bandwidth dimension ratio has been calculated (which is 6246) as a proof for compact size. Thin slots introduced on the feed line provide good impedance matching for whole frequency band. Numerical simulations of the proposed antenna are reported in terms of reflection coefficient ${\leq}-10dB$, vswr 2:1, radiation pattern and group delay (ns). The proposed antenna has advantage of very small size along with better impedance match that provides a practical approach to realize it for BW enhancement and UWB applications.

Influence of Vibration on Freezing and Fermentation of Watery Kimchi (진동이 물김치의 동결과 발효에 미치는 영향)

  • Kim, Gi-Nahm;Han, Sang-Bo;Kim, Eun-Jeong;Lee, Dong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.8
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    • pp.1094-1097
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    • 2007
  • Vibration at frequency of 20 Hz and 0.18 g of acceleration was applied to 600 g watery kimchi contained in a glass jar of 1 L at subfreezing temperature (-3 and $-6^{\circ}C$) and $10^{\circ}C$ in order to see its effect on freezing and fermentation behaviors, respectively. The vibration at the subfreezing temperature delayed the freezing process and contributed to maintaining small ice crystal slurry in subsequent frozen storage. The vibration at $10^{\circ}C$ accelerated the acid and carbon dioxide production from the watery kimchi, which may be beneficial in shortening ripening time and attaining fresh cool taste.

A Study on Channel Access Mechanism of LTE for Coexistence with Wi-Fi on 5 GHz Unlicensed Spectrum (5 GHz 비면허대역 무선랜과의 상호공존을 위한 LTE 시스템의 채널접속방법에 관한 연구)

  • Um, Jungsun;Yoo, Sungjin;Park, Seungkwon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.26 no.4
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    • pp.374-380
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    • 2015
  • With explosion of wireless traffic it is required to further investigate the technologies on acquiring available spectrum resources and on sharing frequency with existing users. In 3GPP, it is started to study on feasibility and functional requirement of LTE standard in order to extend cellular services offered on only licensed band to 5 GHz unlicensed band. Operating scenario on LTE in unlicensed band is focused on carrier aggregation with licensed band, and the coexistence with Wi-Fi services in 5 GHz band is concerned as a major requirement. For a single global solution framework for licensed assisted access to unlicensed spectrum, listen-before-talk(LBT) mechanism of European regulation for fair access to channel under the coexistence environments is currently examined in 3GPP. In this paper, we evaluate two types of LBT, frame based equipment and load based equipment, with considering LTE carrier aggregation feature and performances of file transferred time and throughput.

PHYSIOLOGIC ERUPTION INDUCTION OF TRANSPOSED IMPACTED UPPER INCISORS THROUGH AUTOTRANSPLANTATION (변위매복된 상악전치의 자가이식을 통한 자발적 맹출 유도)

  • Kim, Jae-Gon;Lee, Doo-Cheol;Oh, Kyong-Seon;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.28 no.2
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    • pp.281-286
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    • 2001
  • The treatment method of impacted tooth is various from simple observation to surgical exposure and orthodontic methods, autotransplantation is concerned to severe malposed impacted tooth. Autotransplantation is the transplantation of embedded, impacted, of unerupted tooth, into extraction socket of surgically prepared in the same individual. Autotransplantation of tooth with $\frac{1}{2}{\sim}\frac{3}{4}$ root development provides a good chance of easily extracted, a little complication, pulp survival, and complete root formation. Transplantation of uncompleted root apex tooth is aim to pulpal healing, not endodontic treatment. The case which were treated with autotransplantation is reported, and induced normal physiologic eruption and good dental alignment.

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TREATMENT OF AN IMPACTED DILACERATED INCISOR BY SURGICAL REPOITION (외과적 재위치술에 의한 매복된 만곡절치의 치험례)

  • Seo, Yun-Jin;Lee, Kwang-Hee;La, Ji-Young;An, So-Youn;Kim, Yun-Hee;Keum, Ki-Seok;Lee, Sang-Bong
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.2
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    • pp.166-173
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    • 2012
  • Dilaceration is a developmental disturbance in shape of teeth. It refers to an angulation, or a sharp bend or curve, in the root or crown of a formed tooth. Dilacerations are estimated to occur in 3% of all permanent dentitions. However, the etiology of dilaceration is not yet fully understood. Traumatic injury to the deciduous predecessors and ectopic development of the tooth germ are the two commonly cited causes of this anomaly. The involved tooth is usually the maxillary central incisor and it also shows high prevalence of impaction. At first, We tried to do surgical exposure with orthodontic traction but to no avail. After then surgical repositioning of the dilacerated tooth was performed. Finally, Satisfactory results were obtained at the end of treatment, with improvement in dental esthetics and functional aspects.