• The Hankook-Saengyark Bo
• /
• no.122
• /
• pp.2-2
• /
• 1988

• Korean Journal of Medicinal Crop Science
• /
• v.10 no.2
• /
• pp.144-146
• /
• 2002

Cytogenetic analysis of Anemarrhena ashodeloides BUNGE, which is one of medicinal plants belonging to Haemodoraceae was carried out using Feulgen staining. The somatic metaphase chromosome number was identified to 2n=22 (x=11) and the size of chromosomes ranges from $1.27-3.80\;{\mu}m$. Three pairs of chromosomes were relatively long in total length and the others were short. The karyotype was bimodal in chromosome length and arm ratios. The chromosome complement comprise eight pairs of metacentric (chromosome 2, 3, 6, 7, 8, 9, 10, and 11), two pairs of submetacentric (chromosome 4 and 5), and one pair of subtelocentric (chromosome 1).

• Microbiology and Biotechnology Letters
• /
• v.46 no.4
• /
• pp.360-371
• /
• 2018

Extracts and fractions of Anemarrhena asphodeloides Bunge were prepared and their physiological activities and components were analyzed. Antimicrobial activities of the ethyl acetate and aglycone fractions were $78{\mu}g/ml$ and $31{\mu}g/ml$, respectively, for Staphylococcus aureus and $156{\mu}g/ml$ and $125{\mu}g/ml$, respectively, for Pseudomonas aeruginosa. 1,1-Diphenyl-2-picrylhydrazyl free radical scavenging activities ($FSC_{50}$) of 50% ethanol extract, ethyl acetate fraction, and aglycone fraction of A. asphodeloides extracts were $146.2{\mu}g/ml$, $23.19{\mu}g/ml$, and $71.06{\mu}g/ml$, respectively. The total antioxidant capacity ($OSC_{50}$) in an $Fe^{3+}$-EDTA/hydrogen peroxide ($H_2O_2$) system were $17.5{\mu}g/ml$, $1.5{\mu}g/ml$, and $1.4{\mu}g/ml$, respectively. The cytoprotective effect (${\tau}_{50}$) in $^1O_2$-induced erythrocyte hemolysis was 181 min with $4{\mu}g/ml$ of the aglycone fraction. The ${\tau}_{50}$ of the aglycone fraction was approximately 4-times higher than that of (+)-${\alpha}$-tocopherol (${\tau}_{50}$, 41 min). Analysis of $H_2O_2$-induced damage of HaCaT cells revealed that the maximum cell viabilities for the 50% ethanol extract, ethyl acetate fraction, and aglycone fraction were 86.23%, 86.59%, and 89.70%, respectively. The aglycone fraction increased cell viability up to 11.53% at $1{\mu}g/ml$ compared to the positive control treated with $H_2O_2$. Analysis of ultraviolet B radiation-induced HaCaT cell damage revealed up to 41.77% decreased intracellular reactive oxygen species in the $2{\mu}g/ml$ aglycone fraction compared with the positive control treated with ultraviolet B radiation. The findings suggest that the extracts and fractions of A. asphodeloides Bunge have potential applications in the field of cosmetics as natural preservatives and antioxidants.

• Journal of Sasang Constitution and Immune Medicine
• /
• v.9 no.1
• /
• pp.255-271
• /
• 1997

• Health and Mission
• /
• s.19
• /
• pp.38-40
• /
• 2010

• Korean Journal of Medicinal Crop Science
• /
• v.5 no.4
• /
• pp.266-275
• /
• 1997

Anemarrhena asphodeloides BUNGE is one of important medicinal crops, which has been collected or/and cultivated for its rhizomes. The main medicinal ingredient of the A. asphodeloides Bunge rhizomes is a saponin, which is known to have medical values for diaphoresis, sedatives and biuresis. However, studies on cultural methods and breeding works on this plant have not been made in detail. To increase productivity and to improve quality of crops, it is important to collect cultivated and wild lines, to classify them based on morphological and genetic characteristics, and to select superior pure lines at first. Therefore, total 20 A. asphodeloides lines collected were cultivated at the fieldof Chungnam Provincial Administration of Rural Development in 1995 to study the agronomic characteristics and to classify them based on morphological characteristics. Characteristics related with reproductive organ such as the number of spikes per plant and peduncle length showed greater variations than vegetative organrelated characteristics such as leaf length and rhizome length based on the coefficient of variation. Vigorous shoot growth resulted in better development of reproductive organs such as peduncle length and number of spikes per plant. However, the development of spikes was negatively correlated with chlorophyll content. Characteristics of underground parts were more significantly correlated with spike characteristics than aerial part characteristics. A. asphodeloides tested in this study were classified into 2 groups (group A and group B) based on centroid linkage cluster analysis. Group A showed more vigorous shoot growth with more leaves and spikes per plant, longer peduncle length, thicker peduncle diameter and higher shoot dry weight than group B. Group A could be further classified into 2 sub-groups based on leaf size, spike length and peduncle diameter, while group B also could be classified based on number of leaves, number of spikes and shoot dry weight.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.31 no.2
• /
• pp.11-23
• /
• 2018

목적 : 본 연구에서는 $TNF-{\alpha}$와 $IFN-{\gamma}$로 자극한 인간피부각질형성세포 (HaCaT keratinocytes) 모델을 사용하여 지모가 피부장벽 기능에 미치는 영향을 알아보고자 하였다. 방법 : MTT assay를 통하여 지모 주정(70% 에탄올) 추출물 (EAA)이 HaCaT keratinocytes의 세포생존율에 미치는 영향을 확인하였으며 wound healing assay를 통해 EAA가 HaCaT 세포의 이주 능력에 영향을 주는지 관찰하였다. 또한 western blot analysis와 qRT-PCR을 통하여 EAA가 $TNF-{\alpha}/IFN-{\gamma}$로 자극한 HaCaT 세포에서 iNOS의 단백질 발현 및 IL-4, IL-13, IL-6의 mRNA 발현, filaggrin의 단백질과 mRNA 발현에 미치는 영향을 조사하였다. 결과 : EAA는 처리 농도 $500{\mu}g/ml$까지 HaCaT keratinocytes의 세포생존율에 영향을 미치지 않았다. EAA는 wound healing assay에서 HaCaT 세포의 이주 능력을 증가시켰으며, $TNF-{\alpha}/IFN-{\gamma}$로 자극한 HaCaT 세포에서 iNOS의 단백질 수준을 감소시켰다. 또한 EAA가 IL-4, IL-13, IL-6의 mRNA 발현을 억제하는 것 역시 확인할 수 있었다. 뿐만 아니라 EAA는 $TNF-{\alpha}/IFN-{\gamma}$ 자극에 의해 감소했던 filaggrin을 단백질과 mRNA 수준에서 회복시켰다. 결론 : EAA가 HaCaT 세포에서 Th2 type cytokines, pro-inflammatory cytokine의 억제와 filaggrin 회복을 통해 피부장벽 기능 손상에 대한 억제활성을 갖는 것을 확인하였으며, 이를 통해 EAA가 염증으로 인해 손상된 피부장벽 기능 개선에 효과적일 것으로 사료된다.

• Korean Journal of Plant Resources
• /
• v.10 no.4
• /
• pp.351-359
• /
• 1997

Antifungal activities of the crude extracts of Anemarrhena asphodeloides. Coptis japonica and Phellodendron amurense were tested against Phvtophthora capsici. and the control effect on red-pepper phytophthora hlight and phytotoxicities of red-pepper were investigated. The results were summarized as follows; Mycelial growth and zoosporangial germination of the red-peppcr phytophthora hlight organism P. capsici were inhihited hy thc crude extracts of plant materials. Methanol extracts or plant materials had hctter antifungal activity than water extracts at hoth a room temperature and a hoiling condition. Antifungal activities of three crude extracts were gradually decreased with prolonged storage period. Red-pepper phytophthora hlight was effectively controlled hy the crude extracts of three plant materials. Of these. the crude extract of C. japonica was marvelously effective. Phytotoxic symptom to red-pepper seedling showed hy water cultural method hut not by pot test. Seed germination and radicle growth of red-pepper were inhihited hy the crude extracts of three plant materials. Phytotoxic symptoms in the leaves and fruits of red-pepper were not ohserved with exogenous foliage application of the three crude extracts.

• Journal of Investigative Cosmetology
• /
• v.14 no.4
• /
• pp.455-462
• /
• 2018

This study aimed to investigate the antioxidant, antimicrobial and anti-inflammatory effects of Anemarrhena asphodeloides extracts using supercritical extraction. In the antioxidant test, the total concentration of polyphenol compounds was determined to be 22.58 mg/g (supercritical extraction), 18.75 mg/g (ethanol extraction) and 17.69 mg/g (hydrothermal extraction). The DPPH radical scavenging effects were 89.84% (ethanol extraction) and 64.86% (supercritical extraction) at 2000 mg/L. ABTS radical scavenging ability had an IC50 value at 250~500 mg/L for supercritical extraction, higher than that for hydrothermal and ethanol extractions. At 1000 mg/L, elastase inhibition rates were 64.08% for ethanol extraction and 45.84% for supercritical extraction. The antimicrobial effect of the supercritical extraction was higher than that of hydrothermal and ethanol extractions. NO production with stimulated LPS was decreased in a concentration-dependent manner and, at 20 ㎛/mL, NO production was reduced by 77% for supercritical extraction compared with 46% (ethanol extract) and 63% (hydrothermal extract). Here, we confirmed that superficial extracts of Anemarrhena asphodeloides displayed excellent antioxidant, antimicrobial and antiinflammatory effects and have potential to be used as a safe and natural cosmetic material in the future.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2003.10a
• /
• pp.198-198
• /
• 2003