• Title/Summary/Keyword: 지가 변화

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Ttrosine Hydroxylase in Japanese Medaka (Oryzias latipes): cDNA Cloning and Molecular Monitoring of TH Gene Expression As a Biomarker (송사리 Tyrosine Hydroxylase: cDNA 클로닝 및 생물지표로서의 TH 유전자 발현의 분자생물학적 추적)

  • Shin, Sung-Woo;Kim, Jung-Sang;Chon, Tae-Soo;Lee, Sung-Kyu;Koh, Sung-Cheol
    • Environmental Analysis Health and Toxicology
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    • v.15 no.4
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    • pp.131-137
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    • 2000
  • The release of hazardous waste materials into the environment poses serious risks in humans and ecosystems. The risk assessment of environmental pollutants including hazardous chemicals requires a comprehensive measurement of hazard and exposure of the chemicals that can be achieved by toxicity evaluation using a biological system such as biomarkers. In this report we have tried to develop a biomarker used to elucidate a molecular basis of, and to monitor abnormal behaviors caused by diazinon in Japanese medaka (Oryzias latipes) as a model organism. First, an attempt was made to clone tyrosine hydroxylase gene from Japanese medaka that would be a candidate for a biomarker for neuronal modulations and behaviors. For monitoring experiments at behavioral and molecular biological levels, the fish were treated under different sublethal conditions of diazinon and their behavioral responses were observed . In this study we have successfully cloned a partial TH gene from the medaka fish through PCR screening of an ovary cDNA library. DNA sequencing analysis revealed that the amplified fragment was 327 bp encoding 109 amino acids. Comparing the DNA sequence of medaka TH with other species, TH gene revealed the DNA sequence was completely identical to that of rat TH. In the RT-PCR, 330 Up of mRNA was consistently amplified in all the treated samples including control There were no significant differences in the TH expression level regardless of treating concentrations (1∼5,000 ppb) and time (0∼48 hr) The reason appeared to be that RT-PCR was not performed using through a quantitative analysis normalized against an actin gene expression. Organ or tissue - specific detection of TH activity and mRNA as biomarkers will be a useful monitoring tool for neurobehavioral changes in fish influenced by toxic chemicals. Furthermore, quantitative analysis of locomotive patterns and its correlation with the neurochemical and molecular data would be highly useful in measuring toxicity and hazard ofvarious environmental pollutants.

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A study on the white porcelain in Sangju focus on the literature data (문헌자료를 중심으로 한 상주백자 연구)

  • Kim, Jong-Tae
    • Archives of design research
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    • v.19 no.5 s.67
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    • pp.17-30
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    • 2006
  • The early Joseon Dynasty is very important period of studying about Korean history of ceramics because various kinds of porcelain were produced like celadon, brown porcelain, white porcelain, inlaid white porcelain, blue porcelain etc, $\ulcorner$sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ was published and Saongwon established its branch. Moreover, there also had a sudden political change to Joseon Dynasty, so ceramic industry in Gangjin was closed and artisans in Gangjin scattered to find safer place. One of these place was Imiwueri and Chuhyunri, Joongmohyun in Sangju.(the name of the place in that times, now Modongmyun) Also, Sangju in Gyeongsangbuk-Do is one of the places where can get materials for producing white porcelain, so artisans in Gangjin could migrate to the place during change of regime to Joseon Dynasty which based on Sung Confucianism. In $\ulcorner$Tejongsilrok$\lrcorner$, it mentioned specific areas like Joongmo, Hwaryeong in Sangju, Gyeongsang-Do and we can find out about situations of ceramic production in the early 15 centuries with producing dishes for a Royal family. $\ulcorner$Sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ mentioned Imiwueri and Chuhyunri and Sangju produced the half of porcelains. In $\ulcorner$Gyeongsangdojiriji$\lrcorner$, Sangju takes charge of 8 spots which is one third of ceramic production. $\ulcorner$Gyeongsangdojiriji$\lrcorner$ and $\ulcorner$Sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ were published with same goals at the same year. In $\ulcorner$Gyeongsangdosokchanjiriji$\lrcorner$, there was deleted nine spots which included high, middle, low level compared with $\ulcorner$Seiongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$, and in $\ulcorner$Donggukyeojiseungram$\lrcorner$ there was entirely deleted the locations of porcelain spots and potteries.

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Study of Crustal Structure in North Korea Using 3D Velocity Tomography (3차원 속도 토모그래피를 이용한 북한지역의 지각구조 연구)

  • So Gu Kim;Jong Woo Shin
    • The Journal of Engineering Geology
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    • v.13 no.3
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    • pp.293-308
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    • 2003
  • New results about the crustal structure down to a depth of 60 km beneath North Korea were obtained using the seismic tomography method. About 1013 P- and S-wave travel times from local earthquakes recorded by the Korean stations and the vicinity were used in the research. All earthquakes were relocated on the basis of an algorithm proposed in this study. Parameterization of the velocity structure is realized with a set of nodes distributed in the study volume according to the ray density. 120 nodes located at four depth levels were used to obtain the resulting P- and S-wave velocity structures. As a result, it is found that P- and S-wave velocity anomalies of the Rangnim Massif at depth of 8 km are high and low, respectively, whereas those of the Pyongnam Basin are low up to 24 km. It indicates that the Rangnim Massif contains Archean-early Lower Proterozoic Massif foldings with many faults and fractures which may be saturated with underground water and/or hot springs. On the other hand, the Pyongyang-Sariwon in the Pyongnam Basin is an intraplatform depression which was filled with sediments for the motion of the Upper Proterozoic, Silurian and Upper Paleozoic, and Lower Mesozoic origin. In particular, the high P- and S-wave velocity anomalies are observed at depth of 8, 16, and 24 km beneath Mt. Backdu, indicating that they may be the shallow conduits of the solidified magma bodies, while the low P-and S-wave velocity anomalies at depth of 38 km must be related with the magma chamber of low velocity bodies with partial melting. We also found the Moho discontinuities beneath the Origin Basin including Sari won to be about 55 km deep, whereas those of Mt. Backdu is found to be about 38 km. The high ratio of P-wave velocity/S-wave velocity at Moho suggests that there must be a partial melting body near the boundary of the crust and mantle. Consequently we may well consider Mt. Backdu as a dormant volcano which is holding the intermediate magma chamber near the Moho discontinuity. This study also brought interesting and important findings that there exist some materials with very high P- and S-wave velocity annomoalies at depth of about 40 km near Mt. Myohyang area at the edge of the Rangnim Massif shield.

Establishment of Pre-Harvest Residue Limit for Pyrimethanil and Methoxyfenozide during Cultivation of grape (포도(Vitis vinifera L.) 중 Pyrimethanil 및 Methoxyfenozide의 생산단계 잔류허용기준 설정)

  • Kim, Ji Yoon;Woo, Min Ji;Hur, Kyung Jin;Manoharan, Saravanan;Kwon, Chan-Hyeok;Hur, Jang Hyun
    • The Korean Journal of Pesticide Science
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    • v.19 no.2
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    • pp.81-87
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    • 2015
  • The present study was aimed to predict the pre-harvest residue limits (PHRLs) of pyrimethanil (fungicide) and methoxyfenozide (insecticide) in grape, and to estimate their biological half-lives and residual characteristics. The pesticides were sprayed once on grape in two different fields 10 days before harvest. At the end of 0, 1, 2, 3, 5, 7 and 10 days after application, samples were harvested for further analysis. The residual pesticides were extracted with acetonitrile and partitioned with dichloromethane, and the high-performance liquid chromatography with diode array detector (HPLC/DAD) was employed for the residue analysis. The results obtained in the present study show that the limit of detection of both pesticides were found to be $0.01mg\;kg^{-1}$. The recoveries of these pesticides were ranged between 80.6% and 102.5% with coefficient of variation lower than 10%. The biological half-lives of both pesticides were observed in field 1 and field 2 which shows 7.7 and 7.4 days for pyrimethanil and 5.1 and 6.1 days for methoxyfenozide, respectively. Further, the PHRL of pyrimethanil and methoxyfenozide was found to be $8.90mg\;kg^{-1}$ and $5.51mg\;kg^{-1}$, respectively at 10 days before harvest. Consequently, the present study suggests that the residual amounts of both pesticides will be lower than the maximum residue limits (MRLs) when grape is harvested.

The Influence of Ring-Back-Tone(RBT) on Evaluation of the Phone-call Receiver's Personality II -a comparison study between unknown and known people as the receivers- (통화 연결 음악이 통화 상대자의 개성 판단에 끼치는 영향 II -통화상대자를 알고 있는 경우와 모르는 경우에 대한 비교를 중심으로-)

  • Jeong, Sang-Hoon;Suk, Hyeon-Jeong
    • Science of Emotion and Sensibility
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    • v.11 no.3
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    • pp.313-324
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    • 2008
  • The purpose of the study is to investigate the influence of Ring-Back-Tone(RBT) music on the evaluation of the phone-call receiver's personality in dimensions of Openness, Extroversion, and Neuroticism. In a preliminary test, the subjects listened to 17 RBT music stimuli in random order and assessed the personality associated with liking for each music(N=15). Among the 17 RBTs, three were selected to be used in Experiments I and II and they were distinguished from each other in terms of the three personality dimensions(p<0.001). In Experiment I, the subjects were divided into four groups and were asked to make a call to interview an unknown receiver(N=60). Different RBT music was installed depending on the group to which each subject belonged. It was found that different RBT influences the caller's evaluation of the receiver's personality, supporting Hypothesis 1(p<0.001). Moreover, the ratings of the receiver's were highly correlated with those of the RBT music stimuli in terms of Openness(r=0.722, p<0.001) and Extroversion(r=0.753, p<0.001). In Experiment II, an identical experiment design was applied for a new group of subjects who were acquainted with the receiver(N=40). It was hypothesized that previous knowledge about a person would weaken the RBT effect. The results showed that RBT exerted no effect on the evaluation of the receiver's personality when the caller knew the receiver. It was also found that 12 personality traits, where each of the three personality dimensions is described by four traits, facilitated assessment of the character of the RBT music as well as the personality of the receiver.

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A Study on the Plan for Creating a Youth Entrepreneurship Education Environment (청소년 기업가정신 교육 환경 조성을 위한 방안 연구)

  • Kang, Kyoung-Kyoon
    • 대한공업교육학회지
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    • v.42 no.2
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    • pp.67-88
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    • 2017
  • The purpose of this research was educational needs of experts for revitalizing youth entrepreneurship education and creating effective conditions for such education. The subjects of the survey were chosen 100 teachers who had participated in entrepreneurship-related professional training for teachers were selected and surveyed. A total of 100 questionnaires were collected, of which 92 (92.00%) were used for the analysis. Eight were excluded as they were not properly answered. As for the used survey tool, a total of 8 areas and 30 items were derived from the review of the literature, and the validity of the contents was examined through expert meetings. The data were analyzed using the SPSS (ver. 20.0) statistical program. The analysis was conducted in terms of the required competency level, perceived competency level and educational needs. As for the used analytical methods, first, the averages of the required competency level and perceived competency level were calculated and the education needs were calculated using Borich's formula, and then the averages were compared through paired t-test. The results turned out to be statistically significant (p<.000). The details are as follows: As a result of the calculation of the educational needs the educational needs in all areas turned out to be very high with the average being 4.94 points, which indicates that the teachers strongly feel the need for educational strengthening in relation to entrepreneurship. These results show that all the educational conditions such as entrepreneurship-related curriculum, teacher professionalism, educational environment, educational support and the perception among school community members are insufficient in the current school settings. For the improvement of the current status, the education conditions in the following areas should be improved: the cooperation from school community members including principals, teacher support such as an exclusive responsibility teacher system, the development of an entrepreneurship curriculum, the securing of teacher professionalism through the implementation of the curriculum, teacher training support for the enhancement of their professionalism and the provision of educational environment and facilities. For enhancing the perception of parents and society regarding entrepreneurship, it is necessary to establish the precise concept of entrepreneurship and promote it based on such work.

Effects of the Applications of Slaked Lime and Silicate Fertilizers on the Seedling Growth and Yield of Grass/Clover Mixed Swards on Newly Reclaimed Hilly Soil (신개간 산지토양에서 소석회 및 규산질 비료의 시용이 혼파목초의 초기생육 및 수량에 미치는 효과 비교)

  • Jung, Yeun-Kyu
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.23 no.1
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    • pp.23-30
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    • 2003
  • This pot experiment was conducted to find out the effects of slaked lime($L_{0}$ ; 0.00, $L_1$; 3.75, $L_2$; 7.50, $L_3$; 11.25g/pot) and silicate fertilizers($S_1$; 3.75, $S_2$; 7.50, $S_3$; 11.25g/pot) on the seedling growth and yield of grass/clover mired swards on newly reclaimed hilly soil, and it's differences between both the soil improvers. The results obtained are summarized as fellows: Comparing with the $L_{0}$ treatment without soil improver, the seedling vigour and yields of both forages were markedly enhanced by the application of soil improver. The positive offsets of soil improvers tended to be relative higher on ladino clover than orchardgrass in mixed swards. The seeding vigour, botanical composition, and yield of ladino clover were more markedly influenced by the application of silicate fertilizer than slaked line. The yields of ladino clover were increased 75.8% by the 51 over $L_1$, and 32.0% by the $S_2$ over $L_2$, respectively.

Studies on Application of Organic-Compound Fertilizer on Hilly Mixed Sward Ⅰ. Effect of organic-compound fertilizer application on dry matter yield and botanical composition of grass-clover mixtures (山地草地에 對한 有機質 複合肥料의 施用에 關한 硏究 Ⅰ. 有機質 複合肥料의 施用이 混燔牧草의 乾物收量과 植生變化에 미치는 影響)

  • Park, Geun-Je;Lee, Hyuk-Ho;Shin, Jae-Soon;Lee, Jong-Yeol
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.8 no.2
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    • pp.92-98
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    • 1988
  • With a purpose of finding out the effects of magnesium and boron enriched organic-compound fertilizer application on the dry matter yield, yield components and changes in the botanical composition on the hilly pasture, a field experiment was arranged with five different treatments as a randomized block desigh and lasted from September, 1984 to the end of growing season in 1986. The results obtained are summarized as follows: 1. As a early plant growth and development, winter hardiness, growth vigour and coverate of grasses at the plots with organic-compound fertilizer application were better comparing to single dressing. 2. Average dry matter yields for two years were shown significantly high due to increasing amount of fertilizer. This trend was same both single and organic-compound fertilizer. And dry matter yields with organic-compound fertilizer application of low (8,693.1 kg/ha) and conventional level (12,758.7 kg/ha) were appeared to increase by 10 and 15% than those of single dressing of Low (7,930.6 kg/ha) and conventional level (11,122.6 kg/ha), respectively. But it was not significant difference. 3. Dry matter yield of grasses was significantly gained by increasing amount of fertilizer. The yield of legumes at the plots with organic-compound fertilizer application the yields of grasses were a litle more increased by 8-14%, and legumes were much more gained by 26-29% than those of the same species groups with single dressing, but it was not significant between the differnet kinds of fertilizer in the same fertilizing level. 4. At the grassland management, the rate of legumes tended to dominate at the plot without fertilization gradually. On the other hand, the botanical compositions and the rates of grasses were much better maintained at the plots with fertilization. But the rates of legumes with organic-compound fertilizer application tended to increase a little more than those of single dressing gradually. 5. All of the soil chemical properties after the experiment were much more improved comparing to before the experiment. But the average contents of soil organic matter and available $P_2O_5$ were slightly higher at the plot with organic-compound fertilization, on the other hand, pH and exchangeable cations tended to a little less than those of single dressing.

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Effects of Shipping Temperature and Precooling Treatment of Everbearing Strawberry Cultivars 'Goha' and 'Flamenco' Grown on Highland through Export Simulation (모의 수출 실험을 통한 고랭지 사계성 딸기 'Goha'와 'Flamenco'의 유통 온도 및 예냉 처리효과)

  • Eum, Hyang Lan;Bae, Sang Jun;Hwang, Dae Keun;Yeoung, Young Rog;Hong, Sae Jin
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.202-209
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    • 2014
  • This study was conducted to investigate the effects of low temperature shipping condition and precooling treatments (forced air and room cooling) on everbearing strawberry through export simulation of 'Goha' and 'Flamenco' cultivars. After harvest, it took two days to prepare export procedure such as precooling, sorting, storage, transportation, quarantine and handling, and then everbearing strawberry was carried out shipping at room ($20-25^{\circ}C$) or low ($8^{\circ}C$) temperature conditions. In the case of shipping at room temperature, weight losses of both cultivars were increased up to 10% after 2 days where 'Goha' being 2% higher than that of ' Flamenco'. In 'Flamenco' cultivar, shipping temperature and precooling treatment were not effective in firmness and soluble solids contents during transporting periods. However, in 'Goha' cultivar, room cooling treatment and low shipping temperature were effective in maintaining firmness until 4 days after shipping. Especially titratable acidity was affected by shipping temperature ($P{\leq}0.001$) and precooling treatments ($P{\leq}0.05$) in 'Goha' cultivar. Also shipping temperature under $8^{\circ}C$ delayed coloring and decay incidence of both strawberry cultivars, and precooling treatments of both forced air and room cooling reduced frequency of decay. The shelf life of everbearing strawberry at low shipping temperature was extended more than 4 days compared with shipping at room temperature. Precooling treatment including forced air or room cooling will be useful for the two cultivars when they are transported at low temperature. In 'Flamenco' cultivar, the effect of forced air and room cooling was similar, whereas in 'Goha' room cooling was more effective.

Development of Root Media Containing Carbonized and Expanded Rice Hull for Container Cultivation of Horticultural Crops (팽연왕겨와 훈탄을 포함한 원예작물 용기재배용 혼합상토의 개발)

  • Park, Eun Young;Choi, Jong Myung;Shim, Chang Young
    • Horticultural Science & Technology
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    • v.32 no.2
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    • pp.157-164
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    • 2014
  • Objective of this research was to develop root media containing expanded rice hull (ERH) and carbonized rice hull (CRH). To achieve this, the physico chemical properties of two materials were analysed and blended with peatmoss (PM) or coir dust (CD) with various ratio. Based on the physical properties of the blended materials, 4 root media were selected for future experiment. After the analysis of pH and EC of the selected root media, the kinds and amount of pre-planting nutrient charge fertilizers (PNCF) incorporated into each root medium were varied, and then, final chemical properties of the root media were analysed. The total porosity (TP), container capacity (CC), and air-filled porosity (AFP) were 81.3%. 39.9%, and 41.4% in ERH and 77.6%, 64.1%, and 13.5% in CRH, respectively. The percentage of easily available water (EAW, from CC to 4.90 kPa tension) and buffering water (BW, 4.91-9.81 kPa tension) were 11.37% and 5.27%, in ERH and 17.26% and 14.28% in CRH, respectively. The pH of ERH was 7.1, but it was extremely high in CRH such as 11.2. The EC and CEC were $1.31dS{\cdot}m^{-1}$ and $12.1meq{\cdot}100g^{-1}$ in ERH and $6.53dS{\cdot}m^{-1}$ and CEC 7.79 $meq{\cdot}100g^{-1}$ in CRH, respectively. The ranges of TP, CC and AFP in 4 selected media (PM + ERH, 6:4, v/v; CD + ERH, 8:2; PM + CRH, 7:3; CD+CRH 6:4) were 89.2-90.3%, 67.3-81.8%, and 8.3-21.9%, respectively. The pHs and ECs in root media containing peatmoss such as PM + ERH (6:4) and PM + CRH (7:3) were 4.0-4.3 and $0.33-0.365dS{\cdot}m^{-1}$, whereas those of CD + CRH were 7.4-7.9 and $1.282dS{\cdot}m^{-1}$. The pHs and ECs, however, analysed before and after the incorporation of PNCF in each root medium were not significant different. This result indicated that the incorporated fertilizers in PNCF to adjust medium pH did not dissolve enough to influence medium pH, but it is very normal in root media containing dolomitic lime and sulfur powder in adjusting pH. The Information obtained in this study may facilitate an effective formulation of root media containing rice hulls.