• Title/Summary/Keyword: 증식특성

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Characterization of Avian Adenovirus Associated Virus II. Replication of AAAV in Chicken Embryos (Avian Adenovirus Associated Virus (AAAV)의 특성에 관한 연구 II. 계태아에서의 AAAV의 증식)

  • Rhee, Yong O.;Yates, Vance J.
    • Korean Journal of Veterinary Research
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    • v.20 no.1
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    • pp.11-15
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    • 1980
  • 순수하게 분리 정제된 AAAV를 "helper"인 가금 adenovirus와 공동으로 11일령의 계태아에 감염시켰으며 토끼 유래 특히 항혈청을 사용하여 AAAV의 증식성을 추시하였다. AAAV의 증식은 "helper"와의 공동감염인 경우에만 인정되었으며 "helper"의 양과 AAAV의 증식은 서로 일치하였으나, AAAV와의 공동감염인 경우 "helper"의 증식은 억제되는 경향을 보였다. 이 시험에 공여된 7주의 가금 adenovirus중 6주는 AAAV의 증식을 위한 "helper"능을 부여하였으며 "helper"로써의 기능은 계태아에서의 증식성과 일치하였다. 계태아에 순화된 (CELO) 바이러스를 "helper"로 하여 계태아에서의 AAAV의 증식을 조사한바, $10^5$ PFU의 CELO 바이러스를 "helper"로 하였을 경우 접종후 5일에 최고에 달하였다.

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비산화물 세라믹 핵연료 : 특성, 제조 및 성능

  • Lee, Jeong-Won;Kim, Bong-Gu;Lee, Yeong-U;Son, Dong-Seong
    • Ceramist
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    • v.1 no.2
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    • pp.63-73
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    • 1998
  • 현재 원자력발전에 사용되는 핵연료는 산화물 핵연료가 그 주종을 이루고 있다. 특히, 고속증식로용 핵연료로는 최근까지 혼합산화물 핵연료가 개발, 사용되어 왔다. 그러나 이 산화물 핵연료 못지 않게 경제성 및 안전성 관점에서 이를 능가할 수 있는 핵연료 재료로서 탄화물 및 질화물 등의 비산화물 세라믹 핵연료도 그 동안 꾸준히 연구되어 왔으며, 최근 들어서는 새로운 개념의 Pu 혹은 Minor Actinide burning용 핵연료 후보재료로도 각광을 받고 있다. 이들 비산화물 세라믹 핵연료는 산화물 핵연료에 비해 증식능력이 좋아, 특히 8~12년의 증배시간을 갖는 고속증식로 개발의 가능성을 보여준다. 이는 그 특성이 산화물 핵연료의 경우에 비해 더 높은 중금속(Heavy Metal) 밀도와 열전도도를 갖는 장점 때문이며, 이로 인해 높은 선출력(linear power)을 낼 수 있어 소형 노심의 설계가 가능하다. 본 고에서는 고속증식로 뿐만 아니라 다른 형태의 원자로에의 사용에서도 그 응용가치와 개발의 여지가 충분히 있는 비산화물 세라믹 핵연료의 기초특성, 제조기술, 그리고 그 성능에 대하여 비교, 분석함으로써 앞으로의 핵연료 개발연구에 보다 효율적이고 다양한 방향을 제시하고자 하였다.

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The Growth Characteristics of Candida sp. JY-Cells on Ethanol, Acetic acid and Acetaldehyde Substrate (Ethanol, Acetic acid 및 Acetaldehyde 기질에 대한 Candida sp. JY-5 효모의 증식 특징)

  • Yeehn Yeeh;Jun, Hong-Ki
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.239-243
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    • 1985
  • The growth characteristics of Candida sp. JY-5 were examined on ethanol, acetic acid, or acetaldehyde as sole source of carbon by batch culture. The specific growth rates ($\mu$) of this strain on an ethanol during tile exponential period were changed depending upon the initial concentrations at above 0.5 g/l, but not in the proportion. The highest $\mu$ value was 0.291 hr$^{-1}$ and the maximum growth yield was 61.2% at the concentration of 10 g/l. The $\mu$ values on an acetic acid substrate were constant regardless of the initial concentrations presenting 0.106 hr$^{-1}$ in the highest value. The maximum growth yield was shown as 46.8% at the initial concentration of 10 g/l. The $\mu$ value on an acetaldehyde during the exponential period was 0.063 hr$^{-1}$ and the maximum growth yield was 44.9% at the initial acetaldehyde concentration of 0.2 g/l.

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Characteristics of Coastal Water Quality after Diatom Blooms Due to Freshwater Inflow (담수유입에 의한 식물플랑크톤의 대량번식 후의 연안 수질변동 특성)

  • Lee Young-Sik;Park Young-Tae;Kim Kui-Young;Choi Yong-Kyu;Lee Pil-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.12 no.2 s.25
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    • pp.75-79
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    • 2006
  • In order to study the characteristics of water quality in coastal water and mechanism of phytoplankton blooms, factors of water quality were investigated in diatom bloom area due to rainfall event and no diatom bloom area Diatom blooms occurred after heavy rain and the dominant species were Skeletonema costatum($1,200{\sim}5,000cells/mL$) and Thalassiosira spp.($750{\sim}1,200cells/mL$). In diatom bloom area, water temperature, pH, and dissolved oxygen were observed at higher level than in no diatom bloom area Although these two areas were only 20 meters apart from each other, sharp difference in coastal water quality between two areas was observed. In diatom bloom area, concentrations of nitrogen, phosphorus, and silicate were observed at lower level than in no diatom blooms area. This seems to be due to inflow of much trace metal such as Fe, Mo, Se and so on than nitrogen, phosphorus, and silicate by rainfall events. However, distinct differences in DIN/DIP and $DIN/SiO_2-Si$ between these two areas were not observed.

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Characteristics of Tofu Added with Soybeans Cultured by Mycelia of Pleurotus eryngii (큰느타리버섯 균사체를 증식시킨 콩을 첨가제조한 두부의 품질특성)

  • Lee, Ka-Soon;Kim, Hong-Kyu;Oh, Man-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.8
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    • pp.1038-1044
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    • 2006
  • The effects of the addition of soybeans cultured by mycelia of Pleurotus eryngii (SMP) on the characteristics of tofu were studied. Protein and ash contents of SMP were higher than those of untreated soybeans: 43.23 and 6.34% for treated soybeans, respectively, and 40.42 and 5.90% for untreated soybeans. But lipid and carbohydrate contents of SMP were lower. For minor elements, Mg and Ca contents of SMP were higher and P was lower than control. Tofu could be manufactured when SMP was added below 25% to the untreated soybean. In scanning electron microscopic observation, tofu tended to break down as the ratio of SMP was over 25% to the untreated soybean. The addition of SMP $(5{\sim}15%)$ increased the yield of tofu to $1.5{\sim}3.5%$. In textural characteristics, hardness of tofu increased as the ratio of SMP increased up to 20%. Cohesiveness, chewiness, springiness and gumminess were high in tofu which was made with $5{\sim}15%$ SMP. Savory taste and overall acceptability of the tofu prepared with $5{\sim}15%$ SMP were higher than those of control when evaluated by sensory test.

Growth Characteristics of Paecilomyces tenuipes by the Passage in Liquid Media (액체배지에서 눈꽃동충하초균(Paecilomyces tenuipes)의 계대증식에 따른 증식 특성)

  • Oh, Jeong-Mi;Lee, Jae-Kyung;Koo, Hyun-Na;Woo, Soo-Dong
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.169-174
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    • 2008
  • The growth characteristics of Paecilomyces tenuipes according to the passage in the two kind of liquid media were investigated by comparing the mycelium and conidium formation degrees. The potato dextrose broth medium and the silkworm larvae medium containing the silkworm powder were used as the liquid media, and the potato dextrose agar medium and the brown rice medium containing the powder of silkworm pupa were used as the solid media. The conidium formation degree in liquid media differed by the passages but that in solid media was not. This suggested that the passage in liquid media did not affect significantly the conidium formation in solid media. When the brown rice media were inoculated with the concentration of $1{\times}10^{10}$ conidia/ml, $1{\times}10^8$ conidia/ml and $1{\times}10^6$ conidia/ml, respectively, the conidium formation degrees were similar. This indicated that the optimal inoculation concentration of conidium to the brown rice media is $1{\times}10^6$ conidia/ml.

Controlling Bacterial Regrowth Potential by the Limitation of Nutrients in Drinking Water (영양원의 제한에 의한 수돗물에서의 세균재증식능 억제)

  • Oh, Jung-Woo
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.4
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    • pp.431-437
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    • 2005
  • In this study, the profiles of the bacterial regrowth of indigenous bacteria in tap water and Pseudomonas fluorescence P17 were investigated for cases when carbon (glucose), and/or nitrogen ($NO_3^-$-N), and/or phosphorus ($PO_4^{3-}$-P) were added below sufficient nutrient concentration (SNC) and when carbon sources (glucose and acetate) and nitrogen sources ($NH_4^+$-N and $NO_3^-$-N) were added together. The bacterial regrowth was decreased with limitation of nutrients, and were lowered relatively in the sample, which plural nutrients were limited. In addition, phosphate might be the effective nutrient to control the bacterial regrowth in drinking water because the bacterial regrowth was significantly decreased by the limitation of phosphate. In contrast, the bacterial regrowth was retarded with increasing the concentration of $NO_3^-$-N. For simultaneously adding carbon(glucose or acetate) and nitrogen sources ($NH_4^+$-N and $NO_3^-$-N), the regrowth counts appeared highly in the condition, for both glucose and acetate. And, the regrowth was increased with increasing $NH_4^+$-N concentration as a nitrogen source.

Batch Kinetics of Exo-polysaccharide Production by Submerged Cultivation of Ganoderma lucidum (영지의 액체배양에 의한 세포외 다당 생산의 동력학적 특성)

  • Lee, Shin-Young;Lee, Hak-Su;Park, Heung-Cho
    • The Korean Journal of Mycology
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    • v.27 no.4 s.91
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    • pp.304-311
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    • 1999
  • Batch kinetics during the exo-polysaccharide (EPS) fermentation of Ganoderma lucidum was investigated as a function of different substrates (glucose and starch), substrate concentration $(1{\sim}7%,\;w/v)$ and subculture (3 times). Logistic model for mycelial growth fitted the experimental data better than Monod and two thirds power model. The Luedeking-Pirt equation was adequate to fit the kinetic data of product formation and substrate consumption. The EPS production was strongly non-growth associated, although it was mixed type. The product formation and sustrate consumption by growth associated mechanism decreased as the concentration of glucose increased, while those of the non-growth associated mechanism increased. However, starch medium increased the growth associated and non-growth associated substrate consumption indicating higher availability of substrate. Also, batch culture in starch medium showed the higher specific growth rate and stability during subculture than those in glucose medium. In conclusion, the enhanced EPS production and stability in the subculture was found to be remarkably improved by use of starch as sole carbon source in medium. The maximum mycelium dry weight and EPS production of 9.463 and 10.410 g/l, respectively, were obtained after shake culture of 7 days at $30^{\circ}C$ from the media containing 7% starch.

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Characteristics of the Algal Growth inhibition Substances Produced by Alteromonas sp. SR-14 (Alteromonas sp. SR-14가 생산하는 조류증식 저해 물질의 특성)

  • 김지회;이희정;이태식;김형락;이명숙;장독석
    • Journal of Food Hygiene and Safety
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    • v.14 no.3
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    • pp.270-276
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    • 1999
  • In previous reports, the authors isolated the algicidal marine bacterium, Alteromonas sp. SR-14 and demonstrated its growth inhibition of diatom, Chaetoceros calcitrans (C. calcitrans). In this paper, we studied the effects of cell free culture filtrate of Alteromonas sp. SR-14 on the growth of C. calcitrans, and the characteristics of the algal growth inhibition substance. The culture filtrate of Alteromonas sp. SR-14 grown in peptone broth showed growth inhibition activity against C. calcitrans. The reasonable culture conditions of the bacterium for producing of algal growth inhibition substances were $15~20^{\circ}$ in temperature, 7.0-9.0 in pH and $23~30{\textperthousand}$ in salinity, respectively. The algal growth inhibition activity of culture filtrate was increased from stationary phase in growth curve of Alteromonas sp. SR-14. The molecular weights of algal growth inhibition substances produced by Alteromonas sp. SR-14 were ranged about from 3 KDa to 12 KDa. Among the substances, less than 10 KDa fraction were stable by heating at $100^{\circ}$ for 10 minutes, while more than 10 KDa fraction were heat labile. According to the experimental results, the algal growth inhibition substance produced by the bacterium was not a single compound.

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