• Journal of The Korean Society of Grassland and Forage Science
• /
• v.22 no.1
• /
• pp.51-58
• /
• 2002

PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.

• Journal of Life Science
• /
• v.16 no.6
• /
• pp.877-883
• /
• 2006

An alternative method to remove and recover heavy metals is biosorption based on metal-sequestering properties of natural or biological origin. In this study, the effects of factors such as temperature, pH, initial concentration of lead, and initial amount of biomass on biosorption of lead using Aureobasdium pullulans were investigated. A. pullulans has an excellent selectivity to remove lead than other heavy metals such as cadmium, chromium, nickel in pure and mixed solution. The optimum temperature of biosorption with A. pullulans was $40^{\circ}C$ and the amount of removal increased at high pH. The higher initial lead concentration or the lower cell dry weight, the higher amount of lead was adsorbed. The adsorption isotherm of lead was accorded with Freundlich model. The adsorption capacity and initial adsorption rate of living A. pullulans were about twice higher than that of dead one.

• KSBB Journal
• /
• v.24 no.3
• /
• pp.291-295
• /
• 2009

This study performed verification experiment for the removal of total nitrogen in sewage from a Town M village sewage treatment plant using YPNR processes. The total nitrogen discharged after the denitrification process was maintained at a level of 8-15 mg/L, which results in the total nitrogen removal efficiency above 68% on average. The total nitrogen components in discharged water consisted of 16% of ammonia nitrogen, 6% of nitrite nitrogen, and 77% of nitrate nitrogen, which reaches a 95% nitrification efficiency. Hence, the YPNR advanced treatment process used in this study can be successfully applied to sewage treatment.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.29 no.9C
• /
• pp.1269-1278
• /
• 2004

While the management of digital contents is getting more and more important, many researchers have studied about scene change detection algorithms to reduce similar scenes in the video contents and to efficiently summarize video data. The algorithms using histogram and pixel information are found out as being sensitive to light changes and motion. Therefore, visual rhythm gets used in recent work to solve this problem, which shows some characteristics of scenes and requires even less computational power. In this paper, a new scene detection algorithm using visual rhythm by direction is proposed. The proposed algorithm needs less computational power and is able to keep good performance even in the scenes with motion. Experimental results show the performance improvement of about 30% comparing with conventional methods with histogram. They also show that the proposed algorithm is able to keep the same performance even to music video contents with lots of motion.

• Journal of Adhesion and Interface
• /
• v.3 no.3
• /
• pp.15-21
• /
• 2002

The modified polyethylene(m-PE) films were prepared from aqueous dispersions which were synthesized by reacting poly(ethylene-co-methylacrylate) with aqueous KOH and ammonia solution to attach -COOK, $CONH_2$, and COOH as pendent groups in the polyethylene backbones. Thermal, tensile and heat-seal properties were measured to investigate the effect of the pendent groups. Endothermic peaks on DSC curves were affected by the thermal history of the samples and a melting peak was observed at around $86^{\circ}C$ on the second heating curve while three melting peaks were shown on the first heating curve. A minimum value of tensile strength was shown at 70 mole% of COOK based on pendent groups. Tensile modulus increased with increasing the amide content in pendent groups. The m-PE films were heat-sealed at lower temperature than LDPE films and showed a minimum heat seal strength at around 70 mole% COOK content in pendent groups.

• Journal of Digital Convergence
• /
• v.14 no.6
• /
• pp.21-32
• /
• 2016

Baby boomers who were born right after Korea War make up about 15% of population of Korea and lead an aging society rapidly. They're retiring since from 2012 and going to make up nearly 80% of total of old retirees. Baby Boomers with much higher income and education levels are going to enjoy their retirement as traveling. Therefore, this study invests the relationship between lifestyle and travel product selection of baby boomer consumer. Two big results are as follows. One is that baby boomers progress consumption activity(because they have their own purchasing power) and have health and leisure lifestyle and family lifestyle. The other is when they select travel product, they eventually consider three elements- travel company, transportation, and travel colleague.

• Korean Journal of Microbiology
• /
• v.22 no.1
• /
• pp.49-56
• /
• 1984

Formation and regeneration of protoplasts by Novozym 234 from Kluyveromyces fragilis N100 and Candida pseudotropicalis CBS607 were studied. This enzyme was more effective on cells grown at exponential phase than those at stationary one to convert intact cells into protoplasts. As osmotic stabilizer, ammonium sulphate was suitable for not only protoplast formation but also regeneration in K. fragilis as well as in C. pseudotropicalis. Optimal enzyme concentration was 3mg per ml in K. fragilis and 1~3mg per ml in C. pseudotropicalis, respectively. After the exposure of K. fragilis cells to 3mg per ml of enzyme for 3hr at 30$^{\circ}C$ , approximately 95% of protoplast formation of all observed cells was obgained, while about 100% from C. pseudotropicalis under the same condition was produced. The regeneration frequency of protoplasts by this enzyme was much lower than that by snail enzyme(Glusulase) although Novozym 234 converted cells from above two species into protoplasts free of cell debris effectively, compared with Glusulase. Novozym 234 appears to be suitable for subcellular fractionation to obtain nuclei or other organelles rather than protoplast regeneration.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.18 no.12
• /
• pp.2953-2960
• /
• 2014

In the field of image recognition, circle detection is one of the most widely used techniques. Conventional algorithms are mainly based on Hough transform, which is the most straightforward algorithm for detecting circles and for providing enough robust algorithm. However, it suffers from large memory requirements and high computational loads, and sometimes tends to detect incorrect circles. This paper proposes an optimal circle detection and approximation method which is applicable for inspecting fiber optic connector endface. The proposed method finds initial center coordinates and radius based on the initial edge lines. Then, by introducing the simplified K-means algorithm, the proposed method investigates a substitute-circle by minimizing the area of non-overlapped regions. Through extensive simulations, it is shown that the proposed method can improve the error rate by as much as 67% and also can reduce the computing time by as much as 80%, compared to the Hough transform provided by the OpenCV library.

• The Korean Journal of Zoology
• /
• v.38 no.4
• /
• pp.490-497
• /
• 1995

In vitro tissue culture of fat body of Hyphantria cunea in the medium containing [35S]-methionine reveaied that storage protein-i (SP-1) is taken up into fat body of prepupae and 1-day-old pupae. Using Western blotting and ligand binding method, we were able to identify the protein band of the SP-1 receptor protein. For the partial purification, the membrane proteins of fat body cells were solubilixed with 1% Triton X-1OO and applied to anion exchange chromatography. The results revealed the molecular weight of the receptor protein to be about 80 kl)a in SDSPAGE, and the P1 was estimated to be about 6.1. The mobility of the receptor protein in 8D8-PAGE was highly dependent on both temperature during electrophoresls and the condition of samples whether they were in reducing or nonreducing.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.35 no.6
• /
• pp.666-670
• /
• 2002

To investigate changes of components in salt-fermented sardine, Sardinops melanostictus sauce during 18 months fermentation, various chemical properties were examined at 2$\~$3 months intervals. The degree of hydrolysis increased sharply until 5 months of fermentation and showed the gentle increasement after that. On the other hand, the contents of total and amino nitrogens, total ATP related compounds increased gradually during 18 months of fermentation. The hypoxanthine and uric acid were abundant in ATP related compounds, ranging from $75\%$ to $87\%$. The contents of inosine+hypoxanthine and uric acid were crossed at 13.9 months of fermentation. After 18 months of fermentation, sauce was rich in free amino acids, glutamic acid, aspartic acid, lysine, alanine, threonine in that order.