• Journal of Oral Medicine and Pain
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• v.22 no.2
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• pp.219-232
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• 1997

치아는 성별과 연령의 추정은 물론 혈형 검사와 유전자 검사까지 가능하게 하는 중요한 법의치과학적 자료이다 대부분 치아를 이용한 연구는 핵 DNA가 들어있는 치수에서의 연구로 치수내에는 풍부한 혈액 및 세포가 분포해 있어 핵 DNA가 다량 함유되어 있다. 그러나 순수 상아질에는 핵이 없고 따라서 핵 DNA도 없는 것으로 알려졌지만 치수내에 존재하는 핵 DNA가 상아세관을 통하여 상아질내로 침투할 가능성이 있고 실제 근관치료가 되어 있는 무수치를 감정하게 되는 경우도 있다. 본 연구에서는 이러한 치아중에서도 근관치료를 받은 무수치에서 개인식별에 활용되는 유전자가 검출되는지 여부를 확인하고자 하였다. 40개의 근관치료된 치아상아질에서 DNA출 추출하고 중합효소반응을 이용하여 증폭절편다형(Amp-FLPs)을 실시하고 X-Y homologous amelogenin gene과 STR 유전좌위 F13A01, LPL를 검색하여 다음과 같은 결과를 얻었다. 1. 40개의 근관치료된 치아중 19개에서 DNA가 추출되었다. 2. X-Y homologous amelogenin gene 검색으로 40개의 근관치료된 치아에서 21의 남자 치아중 5개, 19개의 여자치아중 7개 등 모두 12개 치아에서 성별검사가 가능하였다. 3. F13A01 유전자는 43개의 근관치료된 치아중 6개의 치아에서 검색되었으며, 4개의 대립유전자 및 5개의 유전자형을 관찰하였다. 4. LPL_유전자는 40개의 근관치료된 치아중 7개의 치아에서 검색되었으며, 3개의 대립유전자 및 3개의 유건자형을 관찰하였다. 이상의 결과를 종합하여 볼 때 근관치료된 치아상아질에서 중합효소반응을 이용한 성별검사 및 STR 유전자위의 검색은 일부 치아에서만 가능하였으나, 근관치료된 치아들도 개인식별을 위한 법의치과학적 자료로서 유용할 것으로 사료된다.

• Journal of the korean academy of Pediatric Dentistry
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• v.27 no.1
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• pp.151-160
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• 2000

The effect of concentration as factor in cytotoxicity, protein synthesis and alkaline phosphatase activity was compared for pulpotomy medicaments (formaldehyde, formocresol, paraformaldehyde, ferric sulfate) Human pulp fibroblasts were exposed to a range of concentrations(0.01, 0.05, 0.10, 0.25, 0.50, $1.00{\mu}l/ml$) of each agents, for period of 24hrs. The cell activities were evaluated by MTT assay, protein assay and alkaline phosphatase activity examination. The results as follows : 1. After 24hrs culture, pulp fibroblasts adding formaldehyde, formocresol and paraformaldehyde were suppressed cell activities with concentration increasing, but, no depression of cell activities by ferric sulfate. No significant difference was in formaldehyde, formocresol and paraformaldehyde. 2. Protein synthesis by pulpotomy agents were not significant difference in pulp fibroblasts but protein synthesis were a little decreased by paraformaldehyde. 3. Alkaline phosphatase activity was a little decreased by pulpotomy medicaments.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.349-355
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• 2017

Bending work using press dies involves bending a flat blank to a desired angle. The bending produces a flange (the bent part) and a web (the unbent part). The bending line will have a bending angle, and there is an inner and outer bending radius. The minimum inner radius size is determined by the material used. When the inner radius size is too small, there will be excess metal welding, which will cause a crack in the outer radius part. The outer bending radius size cannot be controlled by a bending punch and die block. Types of bending include V-bending, U-bending, O-bending, edge bending, twist bending, and crimping. Z-bending involves two bending lines, which are set on the upper side and under surface of the blank, respectively, and upward or downward bending is used. Z-bending is also called crank bending. Z-bending using this type of die structure will produce a standard inner bending radius. The standard size is the minimum bending radius that represents the angle radius of the bending punch. In industry, there is a need for a sharp edge shape with a very small size (R=0.2mm), but that is not possible when using bending punch and die block. The purpose of this research is to meet the need by development.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.491-501
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• 2021

This study was carried out to investigate the occurrence of seedlings of Korean fir (Abies koreana) in Korean fir forest located on the eastern slope of Mt. Halla National Park. Between June and August in 2016, the systematic sampling method was applied to 125 plots of 5 m × 5 m quadrats and field survey was came out to measure the characteristics of sites and understory vegetation. Correlation analysis was applied to the variables quantified from the data collected. Total number of flora found at the study site is 31 in families, 44 in genera, and 56 in species. The main lower layer vegetation was investigated such as Jeju dwarf bamboo, Toothed club moss (Lycopodium serratum), Pacific fir moss (Lycopodium chinense), Climbing hydrangea (Hydrangea petiolaris) etc. Among these plant species, the vegetation which had a high positive correlation with the frequency and the coverage of seedlings of Korean fir were Jeju dwarf bamboo, Toothed club moss and Pacific fir moss.

• Restorative Dentistry and Endodontics
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• v.24 no.4
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• pp.560-569
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• 1999

Blood supply rather than nerve supply implies pulp vitality. To evaluate pulp vitality clinically, electric pulp test and thermal test which are based on sensory nerve response have been used in addition to many auxiliary data such as past dental history, visual inspection, radiographic examination, percussion, palpation and transillumination test. However, reactivity of the nerves to the stimulation is not synonymous with normalcy. Therefore measurement of pulpal blood flow using a laser Doppler flowmeter became a new trial to test the pulp vitality. The purpose of the present study was to evaluate normal pulpal blood flow level of maxillary teeth in adult to provide a guideline in determining the vitality of dental pulp. Pulpal blood flow was measured in maxillary central and lateral incisors, canines, first and second premolars and first molars of seventy nine adults of 22 - 30 years old using a laser Doppler flowmeter (PeriFlux 4001, Perimed Co., Stockholm, Sweden, 780 nm infrared laser, 1mW). For directly-made splints, silicone rubber impressions were taken directly from the mouth. For indirectly-made splints, alginate impressions were taken from the mouth and stone cast were made. After making depressions on the buccal surfaces of the cast teeth to indicate the hole positions, second impressions with vinyl polysyloxane putty were taken from the cast. Holes for the laser probes were made at the putty impressions 4mm above the gingival level. Laser probe (PF416 dental probe, 1.5mm) was inserted in the prepared hole and the splint was set in the mouth. After 10 minutes of patient relaxing, pulpal blood flow was recorded for 5 minutes on each tooth. The recorded flow was saved in the computer and calculated with a software 'Perisoft' version 5.1. Pulpal blood flow was also recorded in six teeth of five individuals with no response to electric pulp test and cold test, with periapical radiolucency, or with history of root canal treatment to compare with nonvital teeth. The difference between the mean flow values of each group of teeth were analyzed using one-way ANOVA and Duncan's Multiple Range test. The results were as follows: 1. The average pulpal blood flow values of all the tested teeth of each location were between 9 - 16 Perfusion Unit. Pulpal blood flow value was highest in maxillary lateral incisors, followed by first premolars, second premolars, canines, central incisors, and then first molars (p<0.01). 2. In six anterior teeth, indirectly-made splint group showed higher pulpal blood flow values than directly-made splint group (p<0.01). In posterior teeth, however, there was no significant flow value difference between directly-made splint group and indirectly-made splint one (p>0.05). 3. Teeth with vital pulps showed higher signal values than teeth with nonvital pulps (p<0.01), and the flow photographs showed heartbeat-synchronous fluctuations and vasomotions, while those were absent in non vital tooth.

• Journal of Korean Society of Forest Science
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• v.112 no.3
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• pp.280-289
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• 2023

Diverse native tree species are distributed in Korean forests; however, information on their regeneration strategies is limited. This study analyzed seed rain from 2011 to 2013, seedling emergence, seedling survival, lifespan distribution, and the relationship between seedling mortality and herbaceous vegetation for 3 years, from 2012 to 2014, to understand the natural regeneration and early survival characteristics of Fraxinus rhynchophylla Hance in natural broad-leaved forests. Seed rain and emerged seedling density significantly differed among the years (p < .05). An increase in seed rain was followed by an increase in seedling emergence in the following year. Seed rain in 2013 and seedling emergence in 2014 showed a significant correlation (p < .05), confirming the trait of F. rhynchophylla seeds that germinate in the following year after seed production. Seedlings emerged in late spring and early summer. Of the seedlings, 78% emerged in the early summer of 2014. Most seedlings died immediately after emergence. Of the seedlings that emerged in August 2012, 56% died within 2 months, and 38% of those that emerged in July 2014 died within 1 month. Shade and competition against ground vegetation chiefly affected the seedling survival of F. rhynchophylla. Fraxinus rhynchophylla showed mast seeding, with a large number of seed production in a particular year. A surge of seedling emergence followed in the early summer of the next year. However, most seedlings died in the same year of emergence. Fraxinus rhynchophylla has a high seedling mortality, but mast seeding and the resulting high seedling emergence produce a few survived seedlings, enabling the continuation of the F. rhynchophylla population. Efforts and research on the natural regeneration of native tree species are required to effectively manage natural forests in Korea.

• Journal of the Korean Wood Science and Technology
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• v.43 no.1
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• pp.43-51
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• 2015

Wood shrinkage, an important study subject with regard to the use of wood, has long been studied by researchers. However, when the size of a wood specimen is measured, distortion must be taken into account, which can be accomplished by applying external force on the wood specimen. However, when measuring a large number of specimens, this technique can be a lengthy process. If the size is measured and the shrinkage is calculated from images acquired with a flatbed scanner, it is possible to reduce the error in the measurement and to shorten the measurement time because the images of many specimens can be acquired with one scan. To clearly establish the boundary between a wood specimen and the background in a scan, an image threshold method was applied here. The size of a wood specimen measured by means of a scanner image was found to be longer than the value determined with a vernier caliper. The maximum pixel size of a scan image for highly accurate shrinkage calculations compared with the use of a vernier caliper was 0.053 mm/pixel.

• Restorative Dentistry and Endodontics
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• v.32 no.6
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• pp.514-521
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• 2007

The purinoreceptor, $P2X_3$ is a ligand-gated cation channel activated by extracellular ATP. It has been reported that ATP can be released during inflammation and tissue damage, which in turn may activate $P2X_3$ receptors to initiate nociceptive signals. However, little is known about the contribution of $P2X_3$ to the dental pain during pulpal inflammation. Therefore, the purpose of this study was to investigate the expression of $P2X_3$ and its colocalization with TRPV1 to understand the mechanism of pain transmission through $P2X_3$ in the human dental pulp with double labeling immunofluorescence method. In the human dental pulp, intense $P2X_3$ immunoreactiyity was observed throughout the coronal and radicular pulp. Of all $P2X_3$-positive fibers examined, 79.4% coexpressed TRPV1. This result suggests that $P2X_3$ along with TRPV1 may be involved in the transmission of pain and potentiation of noxious stimuli during pulpal inflammation.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.1827-1831
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• 2010

국내의 저수지 비상대처계획수립은 저수용량 100만$m^3$이상의 중 대규모의 댐 및 저수지를 대상으로 수립되고 있다. 반면, 전국 14,208개의 시 군 구 지자체관리 저수지 가운데 80% 이상을 차지하고 있는 30만$m^3$ 미만의 중 소규모 저수지에 대해서는 피해규모의 정량화 방안이 체계적으로 구축되어 있지 못한 실정이다. 이에 본 연구는 중 소규모 농업용 저수지의 붕괴로 인하여 하류부에서 발생하는 피해를 산정하고, 잠재적인 피해액을 예측할 수 있는 홍수피해액 산정방법을 연구하고 인명 및 재산피해를 추정하는 기법을 연구하였다. 홍수피해액 산정에 대한 연구는 1970년대에 치수경제성 분석의 필요성이 대두되면서 원단위법(1993, 건설부, 하천시설기준), 회귀분석법(2001, 건설교통부, 치수사업 경제성분석 개선방안 연구), 다차원법(2004, 건설교통부, 치수사업 경제성분석 연구 방법) 순으로 발전되어 왔다. 원단위법과 회귀분석법의 문제점을 보완하기 위하여 다차원법이 제시되었지만 사용하는 자료가 방대하고 실제 가용성이 제한적인 자료를 포함하고 있어서 적용성이 떨어지는 단점을 가지고 있다. 본 연구에서는 중 소규모의 농업용 저수지 붕괴로 인한 홍수범람시의 피해액산정을 위하여 기존의 원단위법과 다차원법의 장점을 취하여 침수피해추정곡선법(IDEM : Inundation Damage Estimation Method)을 적용하였다. 인명손실액, 이재민피해손실액, 건물피해액, 건물내용물 피해액, 농작물 피해액, 농경지피해액, 공공시설물 피해액으로 구분하고 현재 제공되는 통계자료와 GIS 기법을 이용하여 대상 저수지인 창리저수지의 붕괴에 따른 소규모 범람구역의 침수심별 홍수피해액을 산정 후 침수심-피해액 곡선을 작성하였다. 창리저수지의 경우 저수지 붕괴시 0.4m의 침수심부터 제내지의 침수피해가 발생함을 알 수 있었으며, 침수심-피해액 곡선을 이용하여 침수심별 피해규모를 예상할 수 있었다. 향후 다양한 저수지에 적용성을 검토하여 국내 중 소규모 농업용 저수지 붕괴에 따른 홍수피해액 산정에 이용될 수 있을 것으로 판단된다.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.816-816
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• 2012

최근 우리나라는 기후변화와 이상기후 현상 등의 영향으로 홍수에 대한 위험이 커지고 있다. 홍수에 의해 발생하는 인명 및 재산피해를 최소화하기 위해서는 구조적 및 비구조적 대책 등을 통한 치수정책 수립이 필요하며, 대표적인 비구조적 대책으로는 홍수위험지도(flood hazard map) 제작 등이 있다. 홍수위험지도는 홍수시 침수범위, 침수심 등의 정보를 지도상에 표시하고 있으므로 홍수피해가 발생가능한 공간적 범위를 나타내는 것이다. 홍수위험지도는 침수정보 뿐만 아니라 대피로, 대피처 등 홍수시 피난정보도 함께 나타낼 수 있으므로 홍수발생시 인명 피해를 최소화하기 위하여 활용될 수 있다. 또한 홍수피해 예상범위를 미리 추정할 수 있으므로 피해가능 지역의 특성을 고려하여 구조적 치수계획 수립의 적절한 기준을 제시할 수 있다. 즉, 침수 가능지구 내의 사회적, 경제적, 문화적 중요도를 평가함으로써 지역 특성을 고려한 선택적 홍수방어에 활용될 수 있는 것이다. 본 연구에서는 이러한 중요도 중 홍수피해 예상인구에 대한 현실적인 계산 방법을 제안하고자 한다. 홍수가 발생하여 침수가 예상되는 지역에서 사람이 실제 거주하는 면적을 고려하는 방법은 토지등기부의 지목을 활용하는 것이다. 토지지목은 토지의 종류를 사용목적에 따라 구분한 명칭이며,"측량, 수로조사 및 지적에 관한 법률" 제67조에서 전 답 과수원 목장용지 임야 광천지 염전 대(垈) 공장용지 학교용지 주차장 주유소용지 창고용지 도로 철도용지 제방(堤防) 하천 구거(溝渠) 유지(溜池) 양어장 수도용지 공원 체육용지 유원지 종교용지 사적지 묘지 잡종지 등 28가지 항목으로 구분된다. 여기서 대(垈) 지목은 영구적 건축물 중 주거 사무실 등의 부지를 의미하므로 상주인구가 거주하는 지역으로 가정할 수 있다. 침수가 예상되는 지역에서 대(垈) 지목의 면적을 고려하여 계산된 인구수와 단순 면적비로 계산된 인구수를 비교한 결과, 도시화의 비율이 높은 지역일수록 단순 면적비에 비해 인구수가 높게 계산되었고, 농경지 및 산지가 발달된 지역일수록 단순 면적비에 비해 인구수가 낮게 계산되었다. 따라서 침수가 예상되는 지역의 인구수를 예측할 경우 토지지목 중 대(垈) 지목의 면적을 고려하여 계산하는 것이 보다 합리적인 홍수피해 예상인구를 계산하는 방법으로 판단된다.