• Title/Summary/Keyword: 중성당

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Phenolic Compounds and Antioxidant Activity in Cell Wall Materials from Deodeok (Codonopsis lanceolata) (더덕 부위별 세포벽 물질의 페놀성화합물과 항산화 활성)

  • Kan, Yoon-Han
    • Korean Journal of Food Science and Technology
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    • v.41 no.3
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    • pp.345-349
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    • 2009
  • This study measured the amounts of uronic acid, total sugars, non-cellulosic neutral sugars, phenolic compounds as well as antioxidants activity in cell wall materials (CWM) derived from different parts of deodeok (Codonopsis lanceolata). The values of the uronic acid (UA): neutral sugars (NS) ratio in polymers extracted from the CWM of the flesh and skin were 4 and 6, respectively. The total sugar contents of the flesh and skin were 788.6 and 824.9 ${mu}g/mg$ of CWM, respectively. Galactose and arabinose were the main noncellulosic neutral sugars. The chemical structure of five phenolic compounds from the CWM were analyzed and identified as vanillic acid, p-OH-benzaldehyde, vanillin, ferulic acid, and 8-O-4' diferulic acid by HPLC spectral data. Among them, p-OH-benzaldehyde, vanillin, and 8-O-4' diferulic acid were the first compounds identified from the deodeok. The content of 8-O-4' diferulic acid in the skin CWM was 56.1 ${mu}g/g$ AIR (alcohol insoluble residue). The ethanol-NaOH fractions from CWM had the highest oxygen radical absorbance capacity (ORAC) activities, followed by the AIR fractions and ethanol fractions.

Changes in the Non-cellulosic Neutral Sugars of Cell Wall of Persimmon Fruit by Treatment of Cell Wall-Degrading Enzymes (세포벽 분해효소의 처리에 따른 감과실의 세포벽 구성 비섬유성 중성당의 변화)

  • 김광수;신승렬;송준희;정용진
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.2
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    • pp.247-253
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    • 1995
  • This paper was performed to investigate the changes of non-cellulosic neutral sugars composition in cell wall of persimmon fruit by treatment of cell wall degrading enzyme in vitro. Rhamnose, xylose and galactose in cell wall by polygalacturonase treatment, arabinose, galactose and rhamnose in cell wall by mixed enzyme treatment and arabinose and galactose in cell wall by ${\beta}-galactosidase$ treatment decreased, respectively. Noncellulosic neutral sugars of pectins extracted cell wall by enzyme treatments decreased and those by polygalacturonase treatment decreased remarkably. Rhamnose, arabinose and xylose in hemicellulose I of cell wall by polygalacturonase treatment were higher than those of untreated, and rhamnose and xylose in that by ${\beta}-galactosidase$ treatment were higher but arabinose, mnnose and galactose decreased. Xylose, mannose and glucose in that by mixed enzyme treatment were higher than those of untreatment and arabinose and galactose decreased. Contents of total non-cellulosic neutral sugars in hemicellulose of untreatment, and contents xylose, and glucose in hemicellulose II of cell wall by polygalacturonase treatmet decreased but those of other treatments were not changed.

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Changes in the Non-cellulosic Monosaccharides of Cell Wall Polysaccharides of Persimmon Fruits during Maturation and Postharvest (감과실의 성숙과 추숙중의 세포벽 다당류의 비섬유성 단당류의 변화)

  • Shin, Seung-Ryeul;Song, Jun-Hee;Kim, Soon-Dong;Kim, Kwang-Soo
    • Korean Journal of Food Science and Technology
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    • v.22 no.7
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    • pp.743-747
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    • 1990
  • Glucose, arabinose and total non-cellulosic neutral sugar contents of alcohol-insoluble substance were increased during maturation of persimmon, but arabinose, galactose and total non-cellulosic neutral sugar contents were decreased in soft persimmon. The main non-cellulosic neutral sugars of cell wall were galactose, arabinose and glucose. Arbinose and galactose contents were decreased during maturity and this tend was remarkable in soft persimmon. Pectic fraction contained $70{\sim}82%$ of uronic acid, and galactose, arabinose and uronic acid of pectic fraction were decreased. The main non-cellulosic neutral sugars of hemicelluloses were glucose, xylose, and galactose. Galactose was decreased during maturation and postharvest, and contents of non-cellulosic neutral sugar were decreased in soft persimmon.

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대추의 성숙중에 일어나는 연화현상에 관한 연구

  • 손미애;김광수
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 1994.07a
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    • pp.23-24
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    • 1994
  • 본 연구는 성숙과의 저장중에 세포벽분해효소가 세포벽을 분해해서 물성의 변화를 유발함으로 일어나는 과실의 연과가 품질과 저장성의 저하 뿐만 아니라 영양적, 경제적 손실을 초래한다는 점을 고려하여 대추의 성숙중에 일어나는 연화현상을 연구하고자 성숙에 따른 경도, 세포벽분해효소의 활성, 세포벽 다당류 pectin질, 비섬유성 중성당 및 조직의 변화를 조사하였다. 경도는 대추의 숙성에 따라 감소하였고, polygalacturonase와 $\beta$-galactosidase의 활성은 각각 변색기와 완숙기에 나타난 이후 급격히 증가하였다. 세포벽 다당류인 pectin질과 알칼리 지용성 hemicellulose는 완숙기가지 증가햐였으나 cellulose는 완숙기에 산가용성 hemicellulose와 cellulose를 제외한 세포벽 다당류의 함량은 다소 감소하였다. 대추의 세포벽 비섬유성 중성당으로 rhamnose, arabinose, xylose, mannose, galactose, glucose가 동정되었고, 성숙동안에 pectin질에서는 arabinose, mannose, galactose와 총 비섬유성 중성당의 함량이 감소하였고, 산가용성 hemicellulose에서는 xylose와 mannose가 뚜렸하게 증가하였으나 중성당은 변화없었으며, 알칼리 가용성 hemicellulose에서는 성숙에 따른 변화가 거의 없었다. pectin질의 경우 수용성 pectin, EDTA 용해성 pectin 및 총 pectin은 성숙중에 증가하는 경향이있으나 불용성 pectin은 감소하는 경향이였으며 과숙기에는 불용성 psctin EDTA용해성 pectin 및 총 pectin의 함량은 모두 현저히 감소하였다. 대추의 성숙중 조직에서는 pectin질로 구성된 중충의 붕괴현상이 뚜렸하게 나타났다.발이 절실히 필요한 실정이다. 이러한 배경으로 본 강연에서는 효소적갈변 저해제의 개발과 그들의 식품가공에의 적용 현환 및 화장품, 의약품으로의 응용에 대해 설명하고자 한다.L주에 비해 S주는 수정후 용과가 더 심하다. 9) 화분관의 행동은 수정력과 완전히 일치된다. 즉 L-selfing, $L{\times}L$, S-selfing, $S{\times}S$등의 부적법 수분에서는 화분관은 화주의 미중에서 정지되지만 $L{\times}S$, $S{\times}L$,에서는 수분 약 40-50분 후이면 화분관은 자방까지 도달된다. 10) S주는 웅본으로 오인되어 있지만 인위적법수분을 하면 수정력이나 화분관의 행동은 L주에서와 동일하다. 11) S화분은 완전하지만 L화분은 약 70%가 내용공허한 Adortive pollen 이다. 12) L화분중 나머지 30%도 S화분에 비해서 염색도가 낮은것이 많고 S화분 같이 농염되는 것은 극히 소수이다. 13) 본장물은 분화가 고도로 진행된 전형적인 이형예작물이여 마치 Dimorphism 에서 Dioecious 에로 이행되는 수가 있다는 것을 표시하는 증거가 되는 것 같다. 다소 높은 산소농도 3%~5% 이산화탄소 농도 5~8%에서 저장하는 것이 효과적일 것으로 판단되었다.철쭉군목으로 대표되나 군단이 하의 군목들은 다소 차이를 보이는 것으로 나타났다. 중간상인이론의 수정이 필요하다고 본다.가\ulcorner 본 논문에서는 표면적 형태에도 불구하고 [-wh]의미의 겹의문사는 병렬적 관계의 합성어가 아니라 내부구조를 지니지 않은 단순한 단어(minimal $X^{0}$ elements)로 가정한다. 즉, [+wh] 의미의 겹의문사는 동일한 구성요 소를 지닌 병렬적 합성어([

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Active Transport of Acidic Amino Acids in Suspension Cultured Brassica sp. Cells (배추과 식물현탁배양 세포내에서 산성 아미노산의 능동수송)

  • 조봉희
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.137-142
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    • 1995
  • The acidic amino acids, aspartate and glutamate, which have a negative charge in physiological pH, possess the same transport system as neutral amino acids according to the competitive inhibitory studies with the neutral amino acids. The neutral amino acids cotransported with one H+ per molecule, and one K+efflux per one molecule for charge compensation (Cho,1994), but the acidic amino acids cotransported with two H+ per one molecule, and one K+ efflux per one molecule. The active transport system, which possess the same carrier but cotransported with the different number of H+, reported for the first time. from the results, we can see that one of cotransported H+ protonated at first carboxyl group of pK$_3$ of acidic amino acids, and then as a neutral form cotransported with H+ Therefore, Brassica possess two amino acids transport system for 20 amino acids, namely general - and basic amino acids transport system. The evolutionary meaning of amino acid carriers described with other reported plants.

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Lipid Composition of Kidney Bean (Phaseolus vulgaris L.) (강남콩(phaseolus vulgaris L.)의 지방질 성분)

  • Kwon, Yong-Ju;Uhm, Tai-Boong;Song, Geun-Seoup;Kim, Choong-Ki;Lee, Tae-Kyoo;Yang, Hee-Cheon
    • Korean Journal of Food Science and Technology
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    • v.19 no.6
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    • pp.528-532
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    • 1987
  • Lipids of kidney bean were extracted by the mixture of chloroform-methanol-water (1:2:0.8 v/v), fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography, and the composition of these lipid classes were determined by TLC and GLC. The lipid content of kidney bean was 1.9%, and the lipid was consisted of 48.3% neutral lipids. 7.5% glycolipids and 44.2% phospholipids. Triglyceride was the major component of neutral lipids (64.6%). The major glycolipid and phosphlolipid were esterified steryl glycoside (38.3%) and phosphatidyl choline (32.9%). The major fatty acids of kidney bean lipid were linolenic, linoleic, palmitic and oleic acid. Linolenic acid contents were very high to be 37.1% in total lipid and 50.5% in neutral lipid.

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Effects of Cooking and Drying Methods on the Lipid Content and Neutral Lipid Composition of Shrimp (가열 및 건조방법이 새우의 지방질 함량과 중성지방질 조성에 미치는 영향)

  • Kim, Hyun-Ku;Hawer, Woo-Derck S.;Shin, Hyo-Sun
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.17-24
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    • 1989
  • Effects of cooking and drying methods on the lipids content and neutral lipid component of shrimp, Metapenaeus joyneri, were investigated. Total lipid content of the fresh shrimp was 6.0%(dry basis), which was not changed significantly depending on the cooking and drying methods. Lipid of the fresh shrimp was composed of 36.8% of neutral lipids, 21.5% of glycolipids, and 41.7% of phospholipids. Freeze dried shrimp was substantially higher in neutral lipid contents but lower in glycolipid and phospholipid contents than hot air dried one. Main components of the neutral lipids were triglycerides, free sterols, free fatty acids and esterified sterols. Freeze dried shrimp contained higher triglycerides contents than hot air dried shrimp, whereas free fatty acids content in hot air dried shrimp was greater than that of freeze dried one due to the decomposition of triglycerides to free fatty acids during hot air drying. Major fatty acid composition of the total lipid were palmitoleic acid, eicosapentaenoic acid and docosahexaenoic acid, and that of neutral lipid were palmitic acid, palmitic acid, oleic acid, eicosapentaenoic acid and docosahexaenoic acid in fresh shrimp.

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Analytical Studies on the Lipids in Carotenoprotein purified form Saimo Salar Eggs (연어알에서 분리 정제한 carotenoprotein 지질 성분에 관한 연구)

  • 김재웅;이정복
    • The Korean Journal of Food And Nutrition
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    • v.1 no.2
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    • pp.18-24
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    • 1988
  • 이온교환 크로마토그라피와 젤 여과법으로 연어알에서 carotenoprotein을 충분히 정제하였다. CHCls/MeOH (211)용매로 지질 성분을 추출한 후, Sephadex G-25로 정제하고 silicic acid 컬럼으로 분획하여 중성지질, 당지질, 인지질 그리고 콜레스테롤의 량을 각각 정량하였다. T.L.C.와 HPLC로 개별 성분을 분리, 정량한 결과 중성지질은 tripalmitate와 cholesteryl oleate로 구성되었고, 당지질은 esterified steryglycoside와 소량의 cerebroside, 인지질은 p.c, co, lp.c 및 p.e로 구성 되었음을 밝혔다. 결합당 성분으로서는 galactose만이 검출되었다. 지방산은 palmitate와 oleate가 대부분이었으며, 6종류의 sterol성분을 분리 확인하였다. 지질과 결합된 단백질의 아미노산도 분석하여 이들로부터 carotenoprotein의 본성을 추론하였다.

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