• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.77-77
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• 2002

본 연구는 체세포 및 체세포 핵이식란의 염색체 이상에 체세포의 계대의 영향성을 조사하고자 하였다. 한우 성축의 귀 조직으로 얻어진 세포를 공여세포를 체외성숙 후 제핵된 난자에 핵이식을 실시하였으며, 1.9kv/cm, 20$\mu\textrm{s}$/2times의 전기자극으로 융합후 5$\mu\textrm{g}$/ml의 ionomycin에서 4min, 1.9mM 6-DMAP에서 4h동안 배양함으로써 활성화를 유도하였다. 핵이식란은 CRlaa에서 4일간 배양 후 8 -cell단계에서 중기상의 유도를 위하여 상기 배양액 1ml당 0.05$\mu\textrm{g}$ colcemid에서 6-8시간 더 배양하였다. 이후, 6% Fetal bovine serum이 함유된 1% sodium citrate용액에 20분간 저장처리 후, methanol 5 : aceticacid 1 : distilled water 4로 1차, methanol 3: aceticacid 1 로 조성된 2차, methanol 4 : acetic acid 3 : distilled water 1의 3차고정액으로 1분간 재 침지시켰다. 고정 처리가 완료된 slide는 4% Giemsa용액으로 염색한 후 광학현미경 하에서 핵형 양상을 검경하였다. 체세포의 5계대에서는 684개의 spreads를 검경한 결과 염색체 수는 72%가 정상으로 60개이었고, 24%가 60개 이하였으며 4%가 60개 이상을 보였다. 10계대도 5계대와 비슷하여 71%가 정상, 26%가 60개 이하, 3%가 60개 이상이었고, 15계대에서는 55%가 정상이었고, 30%가 60개이하, 15%가 60개 이상을 보였다. 10계대 까지는 mixoploid의 비율의 변화가 없었으나 15계대에서 현저하게 늘어남을 볼 수 있었다. 또한 체외수정란과 핵이식란의 비교에서는 체외수정란은 250개의 spreads를 검경한 결과 염색체 수는 95.6%가 정상으로 60개이었고, 2.0%가 60개 이하, 2.4%가 60개 이상이었으나, 핵이식란은 204개를 검경하여 88%가 정상이었고, 4.9%가 60개이하, 7.1%가 60개 이상을 보임으로써, 핵이식란이 체외수정란에 비하여 염색체 이상의 비율이 높았다. 따라서 계대에 따라 체세포의 염색체이상의 비율이 상대적으로 증가하고, 체세포 핵이식에 따른 염색체 이상이 생길 수 있음을 알 수 있었다. (이 논문은 농림부 연구비에 의해 수행되었음)

• Korean Journal of Food Science and Technology
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• v.26 no.4
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• pp.359-364
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• 1994

Volatile flavor components from various edible portions of Calystegia japonica $(T_{HUNB})\;C_{HOIS}$ were collected by simultaneous steam distillation-extraction(SDE) method using diethyl ether as solvent. Essential oils were analyzed by gas chromatography(GC) and combined gas chromatography-mass spectrometry(GC-MS). Thirty nine volatile flavor components, including 21 hydrocarbons, 1 aldehyde, 4 ketones, 7 alcohols, 4 esters, 1 acid and 1 miscellaneous one were confirmed in leaves. Twenty six components, including 16 hydrocarbons, 2 aldehydes, 3 ketones and 5 alcohols were confirmed in stems, and 52 components, including 26 hydrocarbons, 2 aldehydes, 5 ketones, 13 alcohols, 1 ester, 1 acid and 4 miscellaneous ones were confirmed in roots. The kinds and amounts of volatile flavor components revealed different patterns depending upon various edible portions. Relatively greater numbers of volatile flavor components were identified in roots compared with other portions of this wild plant.

• 한국해양학회지
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• v.16 no.2
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• pp.57-98
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• 1981

This study deals with the marine dinoflagellates observed in 50 samples collected in the vincinity of Yeosu from August 1979 to May 1980. Forty-nine species were identified and these taxa are attributed to 10 genera, of which 2 are infraspecific taxa. Two families, 3 genera, and 32 species are new records for the Korean coastal waters. Small dinoflagellates recorded in this study are ascribed to the use of volumetric samplers instead of nets. In this area, the armored forms are the most important components of the dinoflagellates (39 taxa). All species are illustrated by photomicroscopy. There are 49 pictures of taxa. An attempt has been made to provide more correct references to each species. There are comparative descriptive information, taxonomic comments, and distributional data for each species.

• Journal of Chest Surgery
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• v.41 no.5
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• pp.640-642
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• 2008

Advanced age is known to be a risk factor for early mortality after heart transplantation and is considered to be a relative contraindication. However, recent studies have shown that there are no significant differences in early and midterm survival rates between older and younger recipients. With rising life expectancy and improvements in medical support, the demand for heart transplantation in elderly patients continues to grow. We present a successful case of heart transplantation in a 78-year-old patient.

• Korean Journal of Microbiology
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• v.53 no.2
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• pp.126-128
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• 2017

Cutibacterium acnes (formerly Propionibacterium acnes) is an anaerobic, Gram-positive rod and that is a normal flora of human skin and mucosal surface as well as an opportunistic pathogen related to acnes vulgaris, sarcoidosis, brain abscess, endocarditis, periodontitis, and endodontic infections. C. acnes KCOM 1861 (= ChDC B594) was isolated from a human jaw osteomyelitis lesion. Here, we present the complete genome sequence of C. acnes KCOM 1861.

• Journal of Food Hygiene and Safety
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• v.27 no.4
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• pp.406-414
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• 2012

This study was designed to investigate the effect of fucoidan on the activation of macrophage and on induction of apoptosis in AGS cell. To measure the activity of macrophages, NO and TNF-${\alpha}$ assays were performed in Raw 264.7 cell. Treatment with fucoidan significantly increased production of NO and TNF-${\alpha}$, indicating activation of macrophages. The result of MTT assay shows that cell viability was significantly decreased in a dose and time-dependent manner. Fucoidan increased to enhance mitochondrial membrane permeability, as well as the cytochrome c release from the mitochondria. Fucoidan decreased Bcl-2 and XIAP expression, whereas the expression of Bax was increased in a time-dependent manner compared to the control. In addition, the active forms of caspase-9 were increased, and the inactivation of Akt was decreased in a time-dependent manner. Caspase inhibitor, z-VAD-FMK, canceled the apoptosis of fucoidan, expression of Bax and caspase-9 were decrease. These results indicate that fucoidan induces activation of macrophage and apoptosis through activation of caspase on AGS cell.

• Current Research on Agriculture and Life Sciences
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• v.1
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• pp.11-18
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• 1983

This study was performed to obtain the basic informations for red dry pepper fruit with more pericarp weight(or in percentage) with a complete diallel cross(excluding reciprocals) using eight cultivars. Heterosis, combining ability and inheritance of the dry red fruit weight and its components(stem, placenta, seed, and pericarp) were evaluated. The results obtained were summarized as follows : Dry weight/fruit and its four antomical components were heavier in the earlier harvest fruit than in that of the later fruit. They showed 1% significance among parents and $F_1s$, and those of $F_1$ were significantly heavier than in parent. All characters in earlier fruit of parent, however, were higher than in later fruit of $F_1$. Dry weight percentage of pericarp to dry weight/fruit was highest followed by seed. Percentage of pericarp in the later fruit was increased while the seed decreased and percentages of stem and placenta were not differed between the earlier and later fruit. $F_1$ hybrids above the higher parent were observed in all characters. Mean heterosis (%) was positive in all characters while mean heterobeltiosis (%) was negative excepting seed and dry weight/fruit. GCA and SCA variances were highly significant, and GCA vaiances were greater than SCA in all characters. The directions of dominance were positive. Partial dominance was shown in stem, complete dominance in placenta, pericarp and dry weight/fruit, and over dominance in seed. The effective genes were estimated as one for stem and placenta, and two for seed, pericarp and dry weight/fruit. Heritabilities in narrow and broad sense were higher.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.28 no.2
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• pp.57-66
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• 2010

In reviewing 'Dongkukesangkukjip(東國李相國集)' a selection of prose and poetry describing Toesikje, the house of Kee Heung-soo, a military official in the middle Goryeo Dynasty, the outcome of the study intended to infer garden scenery and landscape culture managed and possessed by high society at that time is outlined as follows. First, Kee Heung-soo, the owner of the house and Lee Kyu-bo, the author of Toesikje Palryeng(退食齋八詠) are thought to be those who first designated the 8 scenic points and recreated the concept of public enjoyment of into the concept of personal enjoyment in Korea. Second, the spatial configuration of the Toesikje garden is presumed to be in the Gee-Seung-Jeon-Gyul(introduction-development-turn-conclusion) style in which a fairyland is built to enjoy a free and peaceful life, while anticipating coming days and pursuing creation-prosperity-transcendence-return. Third, the viewpoint structure of the scenery Wongyeong(Toesikje), Donggyeong(Youngcheondong), Cheonggyeong(Cheokseojung), Myunggyeong(Dokrakwon), Jingyeong(Yeonmukdang), Sigyeong(Yeoneuiji), Yunggyeong(Nokgunheon), and Hyungyeong(Daehoseok) contains a symbolic universal vision of Palchejigyeongsek(the 8 scenic points) which incorporates Samwon(heaven, earth and water) and Obangwi(orientations), and the harmonization principle of the scenic points where the building and garden are harmonized. Fourth, Je, Dang, and Heon, Jeong(齋, 堂, 軒, 亭) are introduced to the garden, and Geehwayeecho, Jingeumgeesoo, Gasan and Goeseok(stone) were used in a variety of ways. Fifth, it was found that the management of Shinseongyeong and enjoyment of Yoosanggoksu events, with the study and seeking of perfect knowledge and discipline, wandering and rest led to a sublime appreciation of aesthetic beauty and divine glory. Sixth, a miniature garden was built to enjoy the view from above, together with the view in the distance, and a culture of secluded living, along with scenery expressing the heart's desire for Utopia was induced.

• Journal of the Economic Geographical Society of Korea
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• v.17 no.2
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• pp.335-348
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• 2014

This study investigates the technical level and industrial structure for the case of the Gazelle industry in Chungnam Province. As a results of the empirical study, the Gazelle industry of Chungnam Province shows a competitive position in terms of scale and growth capacity, which. in turn, provides income and tax collection effects due to the high level of industrial efficiency. Among the Gazelle industry of Chungnam Province, low-level technology industry, compared to high and middle-level technology industries, accelerates higher level of agglomeration and industrial growth, which provides a rapid change of industrial structure of the province. Theses research results imply that research and development investment in the Gazelle industry is essential and industrial development strategy should be conducted via step-by-step with business scale quotient (BQ) and industrial growth rate (IG). Meanwhile, there should be a serious policy review whether low-level technology industry is better off for the province compared to high and middle-level technology industries. This study expects that regional industrial policy applying the concept of Gazelle industry can accelerate regional development since the Gazelle industry contributes to the high level of income growth and employment creation.

• Geophysics and Geophysical Exploration
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• v.14 no.2
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• pp.127-132
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• 2011

We acquired and interpreted more than 650 km of high-resolution seismic reflection profiles in the Hupo Basin, offshore east coast of Korea at $37^{\circ}N$ in the East Sea (Japan Sea) to image shallow and basement deformation. The seismic profiles reveal that the main depocenter of the Hupo Basin in the study area is bounded by the large offset Hupo Fault on the east and an antithetic fault on the west; however, the antithetic fault is much smaller both in horizontal extension and in vertical displacement than the Hupo Fault. Sediment infill in the Hupo Basin consists of syn-rift (late Oligocene. early Miocene) and post-rift (middle Miocene.Holocene) units. The Hupo Fault and other faults newly defined in the Hupo Basin strike dominantly north and show a sense of normal displacement. Considering that the East Sea has been subjected to compression since the middle Miocene, we interpret that these normal faults were created during continental rifting in late Oligocene to early Miocene times. We suggest that the current ENE direction of maximum principal compressive stress observed in and around the Korean peninsula associated with the motion of the Amurian Plate induces the faults in the Hupo Basin to have reverse and right-lateral, strike-slip motion, when reactivated. A recent earthquake positioned on the Hupo Fault indicates that in the study area and possibly further in the eastern Korean margin, earthquakes would occur on the faults created during continental rifting in the Tertiary.