• Microbiology and Biotechnology Letters
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• v.3 no.1
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• pp.41-48
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• 1975

The chemical components and quality of Takjoo mash were examined on the product brewed by the case of commercial amylase, Lactobacillus bulgaricus and the yeast. 1. Lactic acid was produced at the range of 917~1,350 mg per 100 ml of the mash brewed using wheat flour, corn flour, rice and pressed barley, while succinic acid was formed at the range of 6~32 mg per 100m1 of the mash. 2. Citric acid was formed at the concentration of 6.5mg per 100m1 of the mash on the use of pressed barley, and malic acid formed at the concentration of 1.7 mg and 1.4 mg per 100m1 of the mash respectively, on the use of wheat flour and corn flour. 3. No remarkable differences between the kind of raw materials and commercial amylases and compared with the mash brewed with Nurook were observed in the components of fusel oil formed in the mash. 4. Seventeen kinds of the amino acids of the mash brewed with commercial amylase, Lactobacillus and the yeast were detected and its content was 31mg per 100ml, which is less than that of the conventional takjoo, but the contents of glycine and alanine which pray as the component of seasoning in takjoo were sufficient.

• 대한공업교육학회지
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• v.34 no.2
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• pp.62-86
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• 2009

The major purpose of this study is to contribute to activating the career education. To realize the goal, the study has investigated and analyzed an actual condition of the career instruction, which was conducted by the after-school class in the vocational high school. Based on such a research, it has also diagnosed the problems and suggested the plans in order to improve the troubles and manage the career instruction effectively. There are some summarized results of the study as follows. First, Students hope that as well. Students tend to prefer the career educational program for subject-related further study, career experience courses and getting certificates of qualification in order to choose their proper job. Second, the career instruction of after-school in the vocational school focuses on the entrance of universities and gaining certificates of qualification. Third, students have participated in the career educational program to enter the universities and get some certificates of qualification. Forth, teachers have considered the overwork of teachers attributed to the program and the lack of the cost as a major factor to interrupt the effective progress of the program. Finally, most teachers and students hope to have the program after school during weekday.Therefore, for more effective management of career education in the after-school of the vocational high school, the administrative and financial support should be required, and it is necessary to have good qualified teachers and consistently keep investing the funds in improving the program.

• Microbiology and Biotechnology Letters
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• v.8 no.1
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• pp.33-39
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• 1980

In order to invertigate penicillin acylase produced by a strain of Genus Escherichia, 47 strains of Genus Escherichia were isolated and examined to the extend of the inactivation of penicillins and the ability of 6-amino penicillanic acid (6-APA) production. Among them, 12 strains were found to produce penicillin acylase and to form 6-APA. The strain No. 11 of the isolates was selected to be the most excellent one producing the acylase. Optimum culture conditions for the production of the acylase of the strain were found as follows : pH at 7.6~8.0, time on 18 hrs, temperature at 34 to 38$^{\circ}C$. And effective levels of the medium were found to be contained 0.3% phenylacetic acid, 1.0％ yeast extract, 1.0％ peptone and 0.3％ L-glutamate. And, the production of the acylase by the isolate was strongly inhibited by 1% glycerol and the growth was remarkably retarded by the addition of 1.0% n-butylacetate. The acylase was extracted from the isolate and the crude enzyme of the acylase was prepared and the characteristies of the enzyme were primary examined in optimum pH, temperature, stabilities and reaction time.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.85-93
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• 2020

Despite the development of modern medical sciences, the outbreak and spread of infections continue to threaten populations, and the spread of infections among populations is caused by social contacts. There have only been a few studies on social contacts in the Republic of Korea (hereafter Korea). This study identified the types of social contacts in other countries through a systemic literature review and this helped provide basic data for Korea. Twelve studies were finally selected and then published from 2000 to December 2017. The most common study method was a retrospective contact diary (7cases, 58.3%), and random sampling (9cases, 75%). The number of contacts was 4.9-17.7/day, there were more frequent contacts during the weekdays than on weekends, and the most contacted people were those people that met daily. Physical contact was frequent at home. The highest frequency of contacts was between people 5-19 years old. In case of an epidemic, the spread of infection by this age group could be a concern. Assortative mixing was high in the 5-20 years old group, suggesting that this age group may be more likely to spread infection by contact within the same age group. Therefore, a national-wide survey on social contacts will be needed to predict the spread of infections, and to develop infection prevention based on a domestic basis in Korea.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.8
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• pp.3777-3785
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• 2013

This study aimed to find out adolescents' media literacy in terms of reading, writing, and enjoying media or messages. For the purpose, we analyzed the data garnered from 1,036 students enrolled in 5 middle and 5 high schools, respectively. The analyses of the data are summarized as follows: First, mobile phone was most widely used among others, followed by TV, the Internet, mp3 player, computer or video game, radio, a video or DVD in the order. Second, a gender effect was significant in terms of the variety of activities with the Internet, mobile, and SNS: boys were more active in the use of the Internet while girls carried out more activities with a mobile phone and SNS media. Third, high school students showed more critical attitude towards information on the Website than did the middle school students. Fourth, Girls than boys, and high schoolers than middle schoolers, tended to be more active in the production of contents, self-expression, as well as participating in social and political issues online. Fifth, for the enjoyment experiences, time spent for games took up the most of the students' leisure activities, followed by watching TV and the use of the Internet. The findings of the study were discussed in light of media convergence, with implications for developing media literacy education for the youth.

• KIPS Transactions on Computer and Communication Systems
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• v.6 no.4
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• pp.189-196
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• 2017

BIS(Bus Information System) provides the different information related to buses including predictions of arriving times at stations. BIS have been deployed almost all cities in our country and played active roles to improve the convenience of public transportation systems. Moving average filters, Kalman filter and regression models have been representative in forecasting the arriving times of buses in current BIS. The accuracy in prediction of arriving times depends largely on the forecasting algorithms and traffic conditions considered when forecasting in BIS. In present BIS, the simple prediction algorithms are used only considering the passage times and distances between stations. The forecasting of arrivals, however, have been influenced by the traffic conditions such as traffic signals, traffic accidents and pedestrians ets., and missing data. To improve the accuracy of bus arriving estimates, there are big troubles in building models including the above problems. Hidden Markov Models have been effective algorithms considering various restrictions above. So, we have built the HMM forecasting models for bus arriving times in the current BIS. When building models, the data collected from Sunchean City at 2015 have been utilized. There are about 2298 stations and 217 routes in Suncheon city. The models are developed differently week days and weekend. And then the models are conformed with the data from different districts and times. We find that our HMM models can provide more accurate forecasting than other existing methods like moving average filters, Kalmam filters, or regression models. In this paper, we propose Hidden Markov Model to obtain more precise and accurate model better than Moving Average Filter, Kalman Filter and regression model. With the help of Hidden Markov Model, two different sections were used to find the pattern and verified using Bootstrap process.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.44-52
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• 2005

The study, using sequence analysis and phylogenetic relationship of the fusion protein gene, divided the Korean epizootic isolates of Newcastle disease virus (NDV) into several lineages to determine the molecular epidemiology of the virus. A 695 base pair fragment was amplified by polymerase chain reaction between matrix protein gene and fusion protein gene of 30 Korean NDV isolates, which were isolated from field outbreaks of Newcastle disease between 1949 and 2002. All isolates showed the amino acid sequence 112 R-R-Q/R-K-R116 at the C-terminus of the F2 protein and phenylalanine (F) at the N-terminus of the F1 protein, residue 117. These amino acid sequences were identical to a known virulent motif. The region of the F gene between nucleotides 47 and 435 was compared by phylogenetic analysis. Based on nucleotide sequence, the Korean NDV isolates belonged to genotype III, V, VI and VII corresponding to isolates in 1949, 1982 to 1984, 1988 to 1997, and 1995 to 2002, respectively. These data showed that genotypes of five Korean Newcastle disease epizootics had replaced each other serially (III, V, VI and VII) in chronological order. Further, the five Korean Newcastle disease epizootics were closely related with the Necastle disease panzootics or Newcastle disease epizootics in other countries. Present study showed that the Korean genotype V isolated before 1984 was related with European Newcastle disease epizootics in the 1970s, whereas the Korean genotype VI and VII isolated after 1988 were more closely related with Far East Newcastle disease epizootics, especially Newcastle disease3 epizootics in Japan, Taiwan and China. Since 1988, the genotype VI and VII of Far East origin were dominant in South Korea. That might be due to the increased trade of agricultural products including poultry among Far East Asian countries.

• Ecology and Resilient Infrastructure
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• v.7 no.3
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• pp.171-180
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• 2020

With the increase in industrialization and urbanization, scarcity of space for leisure life has become an important issue. Opportunities such as natural scenery and ecological experiences provided by waterfront spaces around streams are fundamental factors in the development of the community and creation of a hydrophilic park. In the past, on-site surveys have been conducted using human resources to quantify the number of river visitors, but the accuracy of the results was not sufficient owing to limitations in expenses, manpower, space, and time. In this study, to overcome this problem, we estimated the number of visitors using the location information related to hydrophilic parks. The study areas were Samrak Ecological Park and Daejeo Ecological Park located downstream of the Nakdong River. We compared and analyzed the pattern of the visitors by using the large communication data and the visiting pattern based on GPS location information. The GPS location information is based on Google Popular Times and Kakao visitor data. When the GPS location data were used, the pattern for weekday and weekend visitors was clearer than when the large communication data were used. Therefore, it is expected to be similar to the result of GPS location information if the number of visitors is extracted under the condition of precision of pCELL size and residence time of 30 minutes or more when using future communication big data. In addition, if revisions such as the Personal Information Protection Act are made to extract more accurate data, by estimating the number of visitors based on GPS data, more accurate indicators of the number of visitors can be derived.

• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.23-30
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• 1996

대략 36,000 base pairs (bp)의 두 가닥짜리 DNA를 지놈으로 가진 사람 아데노바이러스 (Ad)는 DNA 상동성(相同性) 및 생물학적/생화학적 성격이 특이한 49개의 혈청형이 알려져 있는데, 이들 대부분의 Ad가 영유아군 및 면역능이 저하된 성인에서 치사적 결과를 초래할 수 있다. Ad의 세포향성(向性)(tropism)은 매우 다양하여 종류에 따라 상기도 감염, 각결막염, 영유아 장염등을 유발하는데 최근 Ad의 다양한 병원성에 대한 원인을 분자생물학적 수준에서 규명하려는 노력의 일환으로 지역에 따라 주되게 출현하는 Ad형 규명이 활발히 이루어지고 있다. Ad 동정/확인은 표면을 이루고 있는 group 공통항원인 hexon 단백질을 탐지하는 효소면역 측정법 (EIA)에 의하며, Ad형별은 Ad fiber의 세포독성 중화시험에의 한다. 그러나, 세포독성 중화시험이 엄청난 노동력 및 시간을 요구하면서도 민감도/특이도가 만족스럽지 못하여 이를 개선하기 위하여 검체 또는 세포배양에서 Ad DNA를 추출하여 제한효소 절단형태를 비교하는 방법이 개발되었는데 이는 세포배양에 잘 자라지 않는 바이러스주의 형별뿐만 아니라 지역 분리주들의 지놈 변형주를 관찰하는 분자생물학적/분자역학적 연구에도 도움이 되고 있다. 국내에는 Ad와 관련된 소아장염의 빈도가 rotavirus에 의한 것 다음으로 빈번한데도 Ad40/41외에 주되게 출현하는 장내 Ad형들이 전혀 규명된 바 없고, 한국형 Ad들의 지놈형태가 전혀 보고된 바가 없다. 또한 세계적으로 Ad형별 조사지역이 늘어감에 따라 유아장염과 연관된 Ad 역시 Ad40, 41이 외의 형들이 Ad40, 41을 능가하는 것으로 보고되고 있는 지역도 있으나 국내에서는 Ad40, 41이외의 형들은 그 역학적 중요도가 전혀 알려져 있지 않다. 이로서 본 연구의 목적은 Ad주들에 특이 중화항체를 이용한 세포독성 중화시험과 Ad DNA 절단법을 적용하여 한국형 장내 Ad주들의 형별을 처음으로 시도함과 동시에 1989-1991사이 출현한 Ad들의 유전적 변형을 관찰하려는 것이었다. 두 방법 모두 사용하였을 때 주되게 출현하는 장내 Ad형들은 Ad4l, Ad2, Ad7, Ad5, 및 Ad40이었다. Ad40/41-양성 검체를 제외한 Ad hexon-EIA양성들의 77.5%를 형별 할 수 있었던 Ad DNA의 제한효소 절단방법은 형들간의 교차중화로 특이성이 낮았던 중화방법 (47.5%)보다 매우 효율적이어서 두 가지 방법을 함께 적응하였을 때는 40주중의 81.5%인 35주를 형별 할 수 있었다. 또한Ad DNA 제한 효소 절단방법은 Ad7 변이주 (Ad7b)도 탐지 할 수 있었다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.3
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• pp.364-372
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• 1995

On the outbreak of paralytic shellfish poisoning in April 1993 in most of shellfish harvesting areas in Jinhae Bay, Korea, to clarify the toxin production of causative organism Alexandrium species, 19 axenic clonal isolates established from the benthic resting cysts in three different stations of those culture grounds were subjected to PSP toxin analysis by HPLC. Individual toxin content per cell was highly variable among the strains isolated from a sampling area and originated from an individual cyst. Average toxin contents in those areas revealed higher values of 54-70 fmol/cell. Toxin profiles included C1/C2(epiGTX8/GTX8), GTX1/GTX4 and neoSTX as the major components, and GTX2/GTX3, GTX5, C4, dcSTX and STX as the minor or sporadic ones. neoSTX on the dominant toxins showed not only most diverse compositional changes comprising $5-54 mol\%$ ranges but also no detection on the half of the strains examined, which were implicated in arising of heterogeneity with a genetic trait within a geographical region. When average toxin composition was compared, carbamate toxins comprised large proportions of $57\%,\;54\%\;and\;67\%$ as total toxin in St. 1, St. 2 and St. 4, respectively. These results suggested that an extensive paralytic shellfish toxification in Jinhae Bay could be largely due to the production of highly potent carbamate toxins in the causative dinoflagellate Alexandrium species.