• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.3
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• pp.235-245
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• 2016

In this study, we examined the effects of various molecular weights (1, 10, 50, 100, 660, and 1500 kDa) of sodium hyaluronate (HA), which were prepared by enzyme hydrolysis, on the collagen synthesis, anti-inflammation and skin absorption. These HA did not significantly affect the viability of human dermal fibroblast Hs68 cells. Among them, 1500 kDa, 50 kDa HA most significantly increased collagen production by 59%, and 50% in the Hs 68 cells, respectively. Whereas 1500 and 660 kDa HA hardly pass through mouse transdermis membrane, lower molecular weights (1, 10, or 50 kDa) of HA showed time-dependent increase in skin permeation. HA of 50 kDa showed highest anti-inflammatory effects by reducing nitric oxide and tumor necrosis factor-${alpha}$ production in the RAW 264.7 cells, comparing to other HA (1, 10, and 100 kDa HA). Recently, there is no report about anti-wrinkle and anti-inflammatory effects and skin permeation of different molecular weights HA (1, 10, 50, 100, 660 and 1500 kDa), which were produced by enzyme hydrolysis. These results suggested that 50 kDa HA can be potent candidates for the development of effective anti-aging and anti-wrinkle cosmetic agents. The results of this study demonstrated that among those HA with different molecular weights, 50 kDa HA showed highest anti-inflammatory activity, significant capability to induce collagen synthesis and high level of skin permeation.

• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.101-108
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• 2017

The phenolic contents which were extracted with water and 70% ethanol from O. undulatifolius were 7.7, 10.1 mg/g, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of water and ethanol extracts were 78, 82% at $50{\mu}g/mL$ phenolics, respectively. The 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization activity were 92, 76% at $100{\mu}g/mL$ phenolics. Antioxidant protection factor in water and ethanol extracts at $200{\mu}g/mL$ phenolics were 1.51 and 2.08 PF, respectively. Thiobarbituric acid reactive substance were 84% in water extracts and 99% in ethanol extracts at $50{\mu}g/mL$ phenolics, respectively. The inhibition activity on ${\alpha}-Glucosidase$ was 44% in ethanol extracts at $200{\mu}g/mL$ phenolics. The inhibition activity on ${\alpha}-amylase$ was 37-88% in water extracts at $50-200{\mu}g/mL$ phenolics. The tyrosinase inhibition activity as whitening effect were 82% in ethanol extracts. The elastase inhibition activity were 4, 61% in water and ethanol extracts, respectively. The collagenase inhibition activity of antiwrinkle effect showed an excellent wrinkle improvement effect as 39% in water extracts and 67% in ethanol extracts at $200{\mu}g/mL$ phenolics, respectively. The hyaluronidase inhibition activity as anti-inflammation effect of ethanol extracts was confirmed to 46% of inhibition at $200{\mu}g/mL$ phenolic. The astringent effect of water and ethanol extracts was confirmed to 13, 32% of effect at $200{\mu}g/mL$ phenolic, respectively.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.4
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• pp.259-268
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• 2008

In this study, the antioxidative effects, inhibitory effects on tyrosinase and elastase and components of Castanea crenata leaf were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl radical, DPPH) scavenging activity ($FSC_{50}$) of extract / fractions of Castanea crenata left was in the order: 50% ethanol extract ($13.6{\mu}g/mL$) < ethyl acetate fraction (6.2) < aglycone fraction (2.1). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$ of extract / fractions from Castanea crenata leaf extract / fractions on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity was in the order: aglycone fraction (0.8) < 50% ethanol extract (0.5) < ethyl acetate fraction (0.3). The scavenging activity ($IC_{50}$ for ${O_2}^{{\cdot}\;-}$ (superoxide anion radical) generated by NBT method was in the order: ethyl acetate fraction (145.5) < aglycone fraction (65.5). The protective effects on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethyl acetate fraction exhibited the most prominent cellular protective effect (${\tau}_{50}$, $191.9{\pm}12.2\;min$ at $10{\mu}g/mL$). The inhibitory effect of aglycone fraction ($9.1{\mu}g/mL$) on elastase was higher than oleanolic and ($13.7{\mu}g/mL$). And the inhibitory effect of aglycone fraction ($21.6{\mu}g/mL$) on tyrosinase was higher than arbutin ($226.2{\mu}g/mL$). But 50% ethanol extract rarely exhibited the inhibitory activity on tryosinase and elastase. Flavonoids were contained in Castanea crenata left (96.3 mg / 100 g dried Castanea crenata leaf). And flavonoids contained in ethyl acetate fraction were kaempferol, quercetin, quercitrin, and so on. Quercitrin is the most abundant component. These results indicate that extract / fractions of Castanea crenata can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging free radical and ROS, Castanea crenata leaf extract/ fractions could be used as new cosmeceutical for whitening and anti-wrinkle products.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.5
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• pp.572-580
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• 2017

The contents of phenolic compounds in water and 40% ethanol extracts from Okkwang (Castanea crenata) chestnut bur solid (OCS) were $11.24{\mu}g/50{\mu}g$ solid and $10.28{\mu}g/50{\mu}g$ solid, respectively. The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and 2,2'-azino-bis(3-ethylbenzothiazoline- 6-sulfonic acid) radical decolorization activities of water and ethanol extracts were 85% and 100% as well as 87% and 86% at a solid content of $50{\mu}g/mL$, respectively. The anti-oxidant protection factors (PFs) of water and ethanol extracts at a solid content of $200{\mu}g/mL$ were 1.22 PF and 1.45 PF, respectively. Thiobarbituric acid reactive substance were 83% in water extract and 73% in ethanol extract at a solid content of $200{\mu}g/mL$. The inhibitory activities against xanthine oxidase in water and ethanol extracts were 54% and 43% at a solid content of $200{\mu}g/mL$, respectively. The inhibitory activities against ${\alpha}$-glucosidase were 95% in water extract and 96% in ethanol extract at a solid content of $50{\mu}g/mL$. Tyrosinase inhibitory activity was 27% in ethanol extract at a solid content of $200{\mu}g/mL$. The collagenase and elastase inhibitory activities as anti-wrinkle effect were 93% and 11% in water extract as well as 94% and 56% in ethanol extract at a solid content of $200{\mu}g/mL$. Hyaluronidase inhibitory activity as anti-inflammatory effect of water and ethanol extracts were 96% and 52% at a solid content of $200{\mu}g/mL$, respectively. The results show that extracts from OCS can be used as a functional resource with antioxidant, anti-gout, carbohydrate degradation inhibitory, whitening, anti-wrinkle, and anti-inflammatory activities.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.3
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• pp.691-698
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• 2008

The aims of this study are to search herbal medicines that have anti-aging, anti-wrinkle, anti-oxidant and whitening effects. This is preliminary study as a finding for excellent and specific natural agents which inhibit the skin aging and whitening formation. In this study, the articles and the documents, which have been published in domestically or internationally, have been searched and analyzed. Articles were gathered by taking advantage of Society of Cosmetic Scientists of Korea's documents, Pub med database, Korean Medical Database and Korean Studies Information Service System. Among the many species of herbal medicines, we selected the herbal medicines showing superoxide scavenging activities, and 1.1-diphenyl-2-picrylhydrazyl(DPPH) scavenging effects. The herbal medicines were showed anti-wrinkle effects on Metrixmetallo proteinase (MMP-1) inhibition activity and elastase inhibition activity. Also we found herbal medicines that have highly effect of tyrosinase inhibition, L- DOPA inhibition and melanin synthesis inhibition.

• Journal of Convergence for Information Technology
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• v.10 no.11
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• pp.272-278
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• 2020

This study was conducted to confirm the component and efficacy evaluation of Buxus Koreana by different part. Leaf, stem, root, and fruit were extracted with 70% ethanol, and the contents of polyphenols and flavonoids were analyzed, and the efficacy against anti-inflammatory, moisturizing and wrinkles were confirmed. The contents of polyphenols and flavonoids of B. Koreana were the highest in fruit extracts at 77.32 and 51.91 mg/g, respectively. As a result of confirming the NO production inhibitory effect, the root extracts showed an inhibitory effect of 29.6%, and iNOS significantly decreased as the concentration of the extracts increased compared to the LPS treatment group. As a result of checking the mRNA expression level of MMP1, it was found to be effective in the root and stem extracts. Through the efficacy evaluation of B. Koreana the possibility of use as a cosmetic material was confirmed.

• Journal of Convergence for Information Technology
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• v.9 no.12
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• pp.286-293
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• 2019

In this study, four types of retinol, retinyl palmitate, adenocin, and polytoxylate-dretinamide, which are the ingredients of the Ministry of Food and Drug Safety Notice, included in the study. also we looked at trends in research on Sciadopitys verticillata, Prunella vulgaris, Celosia cristata L., Brazilin, Persicaria hydropiper, Astragalus membranaceus Bunge, Forsythiae Fructus, Lithospermum root, Rheum undulatum L. and Cistanche deserticola Y. C. Ma a natural material that has the efficacy of antioxidant aging. The anti-aging study so far has been found to be centered mainly on collagen production and elastase synthesis inhibition mechanisms. However, given that the aging process of the skin is caused by various ageing processes, it is believed that anti-aging studies using safe and effective natural materials that can help the skin age with various mechanisms should be conducted.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.25 no.2
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• pp.221-227
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• 2021

Recently, interests in mask-type skin care devices using light-emitting diodes have been increasing and optical stimuli at certain wavelengths have been known to have various therapeutic effects, such as skin whitening, acne treatment, elasticity and wrinkle improvement by controlling the exposure to wavelengths of light and irradiation time. In particular, light sources of different wavelengths are applied in masks for the purpose of suppressing skin aging, inducing cell proliferation, and alleviating skin inflammation. In this paper, we developed a light-emitting diode control system that is actively used in skin regeneration masks using a microcontroller. Optical stimuli with different manners were applied to skin fibroblast cells in a single or complex wavelengths, and then confirmed how they are effective in the cell differentiation. In addition, we found a specific wavelength that has a positive effect on cell proliferation rates, and confirm the effectiveness of cell proliferation by image processing based quantitative analysis.

• Journal of the Korean Applied Science and Technology
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• v.40 no.1
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• pp.88-94
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• 2023

Sericin is a protein extracted from the cocoon and has many health benefits. This study was conducted to evaluate the anti-wrinkle and anti-inflammatory activity of sericin derived from cocoon as a cosmetic material. The antioxidant activity of sericin was measured by DPPH and ABTS assays. In addition, cell viability was confirmed in Raw 264.7 cells, which are macrophages, and the anti-inflammatory effect of sericin was investigated using the inflammatory response induced using lipopolysaccharide (LPS). It shows that sericin has antioxidant activity and can be an excellent material for anti-aging and anti-inflammatory cosmetics.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.117-117
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• 2022

표고(Lentinula edodes (Berk.))는 담자균강 주름버섯목 느타리과 잣버섯속에 속하는 식용버섯으로 봄에서 가을에 걸쳐 주로 재배하며, 예부터 식용 및 약용으로 널리 이용되어져 왔다. 우리나라를 비롯하여 일본, 중국 등의 동남아시아 지역뿐만 아니라 최근에는 미국, 캐나다 및 네덜란드 등의 서구 나라에서도 관심이 높으며, 전 세계적으로 중요한 식용 버섯으로써 주목을 받고 있다. 표고는 혈중 콜레스테롤 수준의 감소, 항당뇨, 혈압조절, 면역증강, 간 보호 및 비만 억제 등의 다양한 생리활성이 밝혀져 있어 현대인의 식생활에서 수요가 더욱 증가되고 있는 추세에 있다. 버섯의 생육은 크게 균사의 생장과 자실체의 생육으로 구분되는데, 표고 균사의 배양 기간이 86~123일 정도 소요되며, 자실체의 생육 기간은 10~20일 정도로, 균사의 배양 기간이 훨씬 긴 것으로 나타났다. 버섯의 생육 단계에서 영양, 생식, 생장은 모두 수분으로부터 비롯되며, 생육 조건, 조도, 환기 정도 등에 따라 버섯의 품질은 상당한 차이를 보일 수 있다. 일반적으로 봄에 수확한 표고는 대가 짧고 단단하며 감칠맛이 풍부하며, 가을에 수확한 표고는 대가 길고 육질이 부드럽고 비교적 강수량이 높아 수분함량이 높은 것으로 알려져 있다. 이러한 현상은 표고의 수확 시기에 따라 기후, 온도, 강수량 등의 차이가 이화학적 특성에 큰 영향을 미치는 것으로 생각되어, 본 연구에서는 봄과 가을에 각각 표고를 수확하여 이화학적 특성 및 기능성성분을 비교하였다. 분석결과 가을에 수확한 표고가 봄에 수확한 표고에 비해 수분, β-glucan, ergosterol 그리고 ergothioneine 함량이 유의적으로 높게 나타나, 소재 개발 시에 경제적 경쟁력을 고려했을 때 가을에 수확한 표고 활용이 유리할 것으로 고려된다.