• Title/Summary/Keyword: 주기 성분

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Ethanol Extract from Cnidium monnieri (L.) Cusson Induces G1 Cell Cycle Arrest by Regulating Akt/GSK-3β/p53 Signaling Pathways in AGS Gastric Cancer Cells (AGS 위암세포에서 Akt/GSK-3β/p53 신호경로 조절을 통한 벌사상자 에탄올 추출물의 G1 Cell Cycle Arrest 유도 효과)

  • Lim, Eun Gyeong;Kim, Eun Ji;Kim, Bo Min;Kim, Sang-Yong;Ha, Sung Ho;Kim, Young Min
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.4
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    • pp.417-425
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    • 2017
  • Cnidium monnieri (L.) Cusson is distributed in China and Korea, and the fruit of C. monnieri is used as traditional Chinese medicine to treat carbuncle and pain in female genitalia. In this study, we examined the anti-proliferation and cell cycle arrest effects of ethanol extracts from C. monnieri (CME) in AGS gastric cancer cells. Our results show that CME suppressed cell proliferation and induced release of lactate dehydrogenase (LDH) in AGS cells by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and LDH assay. Cell morphology was altered by CME in a dose-dependent manner. In order to identify the cell cycle arrest effects of CME, we investigated cell cycle analysis after CME treatment. In our results, CME induced cell cycle arrest at G1 phase. Protein kinase B (Akt) plays a major role in cell survival mechanisms such as growth, division, and metastasis. Akt protein regulates various downstream proteins such as glycogen synthase kinase-$3{\beta}$ (GSK-$3{\beta}$) and tumor protein p53 (p53). Expression levels of p-Akt, p-GSK-$3{\beta}$, p53, p21, cyclin E, and cyclin-dependent kinase 2 (CDK2) were determined by Western blot analysis. Protein levels of p-Akt, p-GSK-$3{\beta}$, and cyclin E were reduced while those of p53, p21, and p-CDK2 (T14/Y15) were elevated by CME. Moreover, treatment with CME, LY294002 (phosphoinositide 3-kinase/Akt inhibitor), BIO (GSK-$3{\beta}$ inhibitor), and Pifithrin-${\alpha}$ (p53 inhibitor) showed that cell cycle arrest effects were mediated through regulation of the Akt/GSK-$3{\beta}$/p53 signaling pathway. These results suggest that CME induces cell cycle arrest at G1 phase via the Akt/GSK-$3{\beta}$/p53 signaling pathway in AGS gastric cancer cells.

Comparative Study on the Composition of Floral Volatile Components in the Flowering Stages of Robinia pseudoacacia L. (아까시나무(Robinia pseudoacacia L.) 꽃의 개화 단계별 향기성분 조성 비교)

  • Jung, Je Won;Lee, Hyun Sook;Noh, Gwang Rae;Lee, Andosung;Kim, Moon Sup;Kim, Sea Hyun;Kwon, Hyung Wook
    • Journal of Apiculture
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    • v.32 no.3
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    • pp.139-146
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    • 2017
  • Floral scent emitted from many plants is the critical factors for pollinator attraction and defense for adaptation in environments. The fragrance components of flowers are different in composition by geographical origins, climate factors and the development stages of flowers. In the present study, we investigated the volatile-floral compounds in flowers of Robinia pseudoacacia L. and defined the chemical contribution for flowering periods. The volatile compounds analysis was performed by gas chromatography with mass selective detector after solid phase microextraction (SPME). We reported different compositional features of fragrance compounds according to flowering periods. The abundant compounds identified in stage 1 were ${\alpha}$-pinene (66.80%) and ${\beta}$-pinene (26.53%). Those of the stage 2 were (Z)-${\beta}$-ocimene (37.57%), ${\alpha}$-pinene (15.16%), benzaldehyde (16.63%), linalool (12.13%). The volatiles of stage 3 comprised an abundance of (Z)-${\beta}$-ocimene (64.94%), ${\alpha}$-pinene (9.84%), linalool (8.92%), benzaldehyde (1.71%). Leaf volatiles were distinct from those in the reproductive plant parts by their high relative amount of (E)-${\beta}$-ocimene (23.50%) and (Z)-3-Hexenyl acetate (27.87%). Differences in flower scents of the different stages and leaves are discussed in light of biochemical constraints on volatile chemical synthesis and of the role of flower scent in evolutionary ecology of R. pseudoacacia.

A study on collecting representative food samples for the 10th Korean standard foods composition table (국가표준식품성분 데이터베이스 대표시료 선정을 위한 표본설계)

  • Kim, Jinheum;Hwang, Hae-Won;Cho, Yu Jung;Park, Jinwoo
    • The Korean Journal of Applied Statistics
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    • v.33 no.2
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    • pp.215-228
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    • 2020
  • Under Article 19, Paragraph 1 of the Food Industry Promotion Act, Rural Development Administration renews the Korean foods composition table every five years. Before the publication of the tenth revision of the Korean foods composition table in 2021, this paper suggests methods for collecting representative samples of 182 highly consumed foods in Korea. Food markets are categorized by their distribution channels, which are supermarkets and local markets. Eight samples are collected from each category by applying the National Food and Nutrient Analysis Program (NFNAP)'s stratified multi-stage sampling. The NFNAP was implemented in 1997 as a collaborative food composition research effort between the National Institute of Health (NIH) and the US Department of Agriculture (USDA) to secure reliable estimates for the nutrient content of food and beverages consumed by the US population. Selected supermarkets for selecting representative food samples are Emart Kayang, Homeplus Siheung, Lottemart Dongducheon, Emart Suwon, Lottemart Dunsan, Lottemart Yeosu, Emart Ulsan, and Hanaroclub Ulsan. Selected local markets also are Doksandongusijang in Geumcheon-gu and Pungnapsijang in Songpa-gu, Seoul, Ilsansijang in Ilsanseo-gu, Goyang, Unamsijang in Buk-gu, Gwangju, Beopdongsijang in Daedeok-gu, Daejeon, Bongnaesijang in Yeongdo-gu and Jwadongjaeraesijang in Haeundae-gu, Busan, and Jungangsijang in Jinhae-gu, Changwon.

Monitoring on Chemical Properties of Bench Marked Paddy Soils in Korea (우리나라 논토양(土壤)의 화학적(化學的) 특성(特性) 분석(分析))

  • Jung, Beung-Gan;Jo, Guk-Hyun;Yun, Eul-Soo;Yoon, Jung-Hui;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.3
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    • pp.246-252
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    • 1998
  • A survey was conducted to investigate the status of soils involving pH, electrical conductivity, total organic matter content, available phosphate and major exchangeable cations in the paddy soils sampled from 1,168 sites throughout the country. The content of soil chemical properties was lower on the average than the optimum levels for cropping. An average value showed pH 5.6, organic matter $25g\;kg^{-1}$, available phosphate $128mg\;kg^{-1}$, available silicate $72mg\;kg^{-1}$, and exchangeable potassium and the calcium and magnesium were 0.32, 4.0, $1.2cmol^+\;kg^{-1}$, respectively. Soil chemical properties was related with topography except soil pH. A soil pH and organic matter, available phosphate, exchangeable potassium increased with time while exchangeable calcium, magnesium available silicate decreased with time.

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Development of Irreversible Micro-size Ferromagnetic Structures by Hydrogenation and Electron-beam Lithography (수소화 및 전자빔 사진식각 기술에 의한 비가역적 마이크로 크기의 강자성 구조체 개발)

  • Yun Eui-Jung
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.43 no.1 s.343
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    • pp.7-12
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    • 2006
  • In this study, we developed irreversible and stable micro-size ferromagnetic structures utilizing hydrogenation and electron-beam lithography processes. The compositionally modulated (CM) Fe-Zr thin films that had average compositions $Fe_XZr_{100-x}$ with $x=65-85\%$ modulation periods of similar to 1 nm, and total thicknesses of similar to 100 m were prepared. The magnetic properties of CM Fe-Zr thin films were measured using a SQUID magnetometer, VSM and B-H loop tracer. After hydrogenation, the CM films exhibited larger magnetic moment increases than similar homogeneous alloy films for all compositions and かey showed largest increase in $Fe_{80}Zr_{20}$ composition. After aging in air at $300^{\circ}K$ the hydrogenated $Fe_{80}Zr_{20}$ CM films showed much larger magnetic moment increases, indicating that they relax to a stable, irreversible, soft magnetic state. The selective hydrogenation through electron-beam lithographed windows were performed after the circle shaped windows were prepared on $Fe_{80}Zr_{20}$ CM films by electron beam lithography. The hydrogenation through electron-beam resist and W lithographic techniques give a $49\%$ magnetic moment increase. This method can be applied to nano scale structures.

Design and Performance Analysis of the Efficient Equalization Method for OFDM system using QAM in multipath fading channel (다중경로 페이딩 채널에서 QAM을 사용하는 OFDM시스템의 효율적인 등화기법 설계 및 성능분석)

  • 남성식;백인기;조성호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.6B
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    • pp.1082-1091
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    • 2000
  • In this paper, the efficient equalization method for OFDM(Orthogonal Frequency Division Multiflexing) System using the QAM(Quadrature Amplitude Modulation) in multipath fading channel is proposed in order to faster and more efficiently equalize the received signals that are sent over real channel. In generally, the one-tap linear equalizers have been used in the frequency-domain as the existing equalization method for OFDM system. In this technique, if characteristics of the channel are changed fast, the one-tap linear equalizers cannot compensate for the distortion due to time variant multipath channels. Therefore, in this paper, we use one-tap non-linear equalizers instead of using one-tap linear equalizers in the frequency-domain, and also use the linear equalizer in the time-domain to compensate the rapid performance reduction at the low SNR(Signal-to-Noise Ratio) that is the disadvantage of the non-linear equalizer. In the frequency-domain, when QAM signals, consisting of in-phase components and quadrature (out-phase) components, are sent over the complex channel, the only in-phase and quadrature components of signals distorted by the multipath fading are changed the same as signals distorted by the noise. So the cross components are canceled in the frequency-domain equalizer. The time-domain equalizer and the adaptive algorithm that has lower-error probability and fast convergence speed are applied to compensate for the error that is caused by canceling the cross components in the frequency-domain equalizer. In the time-domain, To compensate for the performance of frequency-domain equalizer the time-domain equalizes the distorted signals at a frame by using the Gold-code as a training sequence in the receiver after the Gold-codes are inserted into the guard signal in the transmitter. By using the proposed equalization method, we can achieve faster and more efficient equalization method that has the reduced computational complexity and improved performance.

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A Study on the Correlation between Frequency Components and Sound Quality of a Vacuum Cleaner using the Orthogonal Array (직교배열표를 이용한 진공 청소기의 음질과 주파수 특성의 상관 관계에 관한 연구)

  • Lim Do-Hyeong;Jeong Hyuk;Ih Jeong-Guon
    • Proceedings of the Acoustical Society of Korea Conference
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    • spring
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    • pp.295-298
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    • 2000
  • 제품의 음질 특성과 관련된 주요 주파수 대역을 파악하기 위해 본 연구에서는 청소기 소리의 주파수 대역을 음성인식에 중요한 주파수 대역인 4개의 대역으로 나누고, 각 대역 성분을 직교배열표에 따라 가감한 16개의 소리를 만들었다 만들어진 소리에 대해 10명을 대상으로 4가지의 표현어로 Semantic Differential Method(SDM)로 주관적 평가를 하여, 청소기음의 주파수 특성과의 상관관계를 살펴보았다. 불쾌한 느낌과 관련이 깊은 주파수 대역은 고주파수 대역이었으며, 성능이 좋은 느낌을 주기 위해서는 저주파수 대역을 증가시키는 것 이 효과적이었다.

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Maneuvering pattern Analysis Algorithm for Maneuvering Target base on FCM (퍼지 클러스터링에 의한 기동표적의 기동패턴 분석 알고리즘)

  • Son, Hyun-Seung;Park, Jin-Bae;Joo, Young-Hoon
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.1924-1925
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    • 2011
  • 본 논문에서는 비선형 기동을 하는 기동표적의 추정된 잡음을 분석하여 표적의 기동패턴을 분석하는 알고리즘을 제시하고자 한다. 기동표적의 추정위치와 측정치에서 발생하는 잡음을 가속도와 순수 잡음으로 분리하고 분리된 성분을 분석하여 표적의 기동 패턴을 인식하고 동시에 추적을 실시하는 알고리즘을 구성한다. 잡음의 분리는 퍼지 클러스터링(FCM : Fuzzy C-means Clustering) 기법을 이용하여 적절한 추정값을 이용한다. 추정된 표적의 속도와 가속도, 잡음을 재 구성하여 기동표적의 기동패턴을 분석하고, 동시에 추적을 실시한다. 위의 과정을 통해 가속도를 분리한 후 비선형성을 지닌 기동표적의 기동패턴을 선형화 하여 칼만필터를 이용 잡음을 분리하고 가속도를 다시 보상하여 추적 알로리즘을 구성한다. 그리고 제안된 알고리즘의 수행 가능성을 보여 주기 위하여 몇 가지 예를 제시하였다.

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The Implementation of Information Management System for Eddy Current Testing Evaluation Result (와전류 탐상검사 평가결과 정보관리 시스템 구현)

  • Shin, Jin-Ho;Yi, Bong-Jae;Song, Jae-Ju
    • Proceedings of the Korea Information Processing Society Conference
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    • 2001.10b
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    • pp.1229-1232
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    • 2001
  • 코일에 고주파전압을 걸면 교류자계가 발생하며 이 자게 내의 금속재료에 와전류가 발생하는데 와전류는 재료의 재질, 결함, 이종금속, 형상변화 등에 의해 그 발생상태가 다르기 때문에 검출용 코일에서 얻어진 신호성분을 해석함으로써 재료의 비파괴검사가 가능하다. 원자력발전소에서는 증기발생기와 열교환기를 정기적으로 이 와전류 탐상검사를 수행하고 결함상태를 평가하여 그 결과를 대용량 Media에 저장한다. 본 논문에서는 평가결과 정보를 추출하고 변환 처리하여 RDBMS에 입력 관리하는 과정과 다양한 형태의 열교환기 Tubesheet를 편집하는 기능, 사용자의 검색조건 선택에 따라 데이터베이스를 Query하여 Tubesheet에 Mapping 처리하는 기능, 검사주기 및 기기별로 평가결과를 비교 분석할 수 있는 통계/보고서 기기사양정보 및 검사정보관리 등 제반 Application 시스템 구현 결과를 소개한다.

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A Study on the Improvement of the Sound Quality of the Interior Noise of A/T Vehicle in Idle State (공 회전시 자동변속기 차량의 실내소음 음질 개선에 관한 연구)

  • 이상권;최병욱;여승동
    • Transactions of the Korean Society of Automotive Engineers
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    • v.1 no.1
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    • pp.110-119
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    • 1993
  • 본 논문은 자동변속기를 탑재한 차량에서 에어콘(air condition)을 작동시키고, 공 회전시 기어의 변속을 "D"단에 두었을 때 실내에서 발생하는 이상음의 원인규명 및 해결 방 법에 관한 연구 결과를 논하고자 한다. 이 이상한 소음의 원인을 규명하기 위하여 실린더 내부의 연소압력, 메인 베어링캡(main bearing cap)의 진동, 엔진 마운팅 보스의 진동 및 차 량의 실내소음을 동시에 측정하여 분석하였으며 이 결과에 의하면 이상음의 원인은 크랭크 샤프트(crank shaft)의 굽힘진동이 파워플랜트(power plant)를 가진하여 진동을 증가시키고, 이 진동이 마운팅 보스를 통하여 차량의 차체에 전달되며, 차체의 진동에 의해서 발생하는 고체 전달음(structure-borne noise)이었다. 또한 이상음의 주기는 주파수 성분은 200-400Hz 이었다. 이 이상음은 크랭크 샤프트의 댐퍼 풀리의 질량을 저감하여 크랭크 샤프트의 동특 성을 개선함으로서 해결가능하고, 혹은 점화시기를 지연하여 연소 압력을 낮춤으로서 해결 가능하다.

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