• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.39-39
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• 2022

기온, 강수량 등 기후변화에 따른 식물 멸종이 증가함에 따라 기후변화에 민감한 북방계식물의 중요성이 증대되고 있는 실정이다. 본 연구 대상종의 큰금매화(Trollius chinensis)는 북한 및 북방계 식물로 고산 초지에서 자생하는 미나리아재비과 여러해살이풀이며, 기후변화에 직·간접적인 영향을 받고 있는 실정이다. 이에, 큰금매화의 발아조건을 규명하여 종 복원과 대량증식법 개발을 위한 기초자료를 확보하는 데 목적이 있다. 종자의 형태학적 특성은 원형~계란형(circular~obovate)에 작은 돌기가 겹겹이 있는(Colliculate) 형태였으며, 종피는 진갈색이다. 크기는 길이 1.37±0.741mm, 너비 0.81±0.531mm이었다. 종자의 단면을 확인한 결과, 배는 작은 선형태(Axial-miniature)이었으며, 종자는 91%의 충실도를 보였으며 TZ 테스트에서 모두 배가 전체적으로 붉은색으로 염색되어 양호한 활력을 보였다. 처리구별 발아실험 전 종자 소독은 파종 전 70% 에탄올 처리 후 2%의 NaOCl을 넣어 10분 동안 소독 후 멸균수로 5회 수세하고 멸균수를 제거하여 침지처리에 사용하였다. 발아실험 결과 종자의 최종발아율(FGP)은 15/6℃ GA3 1000mg·L^-1 처리에서 40.0%로 가장 높았다. 평균발아일수(MGT)는 15/6℃ 무처리에서 22.8일로 가장 높았으며, 최종발아일의 평균은 약 9.5일로 확인되었다. 대부분 GA3 처리에서 최종발아율(FGP)이 20% 이상 높게 나타났으며, GA3 500mg·L^-1 보다는 GA3 1000mg·L^-1의 조건에서 3.3~5.5% 최종발아율(FGP)이 향상된 것으로 확인되었다. 한편, 온도 조건 25/15℃ 무처리에서는 발아가 확인되지 않았다.

• Korean Journal of Agricultural Science
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• v.15 no.1
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• pp.47-68
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• 1988

This study was conducted to define the optimal conditions for embryo growth during seed stratification and for breaking dormancy as well as seed germination of stratified ginseng seeds. The experiments were also carried out to detect some materials which were expected to induce seed dormancy in the ginseng seeds. The results summarized as follows; 1. The growth of embryo during seed stratification was significantly inhibited by the existence of endocarp. The fastest embryo growth was resulted at $15^{\circ}C$ and an estimated optimal temperature for embryo growth was about $18^{\circ}C$. 2. There was no significant difference between the embryo growth and germination ratio of ginseng seeds which were sown in seed bed at Aug-5 without seed stratification and that of artificial seed stratification. 3. Embryo growth and germination ratio was significantly inhibited by high temperature treatment at $30^{\circ}C$ for 24 hours or respiration stress by immersing seeds in water for 10 days or more. 4. When the seed stratification was started at $10^{\circ}C$, growth of embryo in the ginseng seeds were almost stopped. But, when the seeds were stratified first at $20^{\circ}C$ for 50 days and next at $10^{\circ}C$ for 50 days, the embryo growth was significantly promoted compared with the embryo growth in the seeds which were stratified at $20^{\circ}C$ for 100 days. 5. The successive embryo growth after seed stratification was significantly accelerated at $10^{\circ}C$ but the seeds chilled at $5^{\circ}C$ for 100 days were resulted in the highest germination ratio as well as the shortest days for germination. 6. The successive embryo growth during chilling treatment and seed germination were significantly inhibited by immersing seeds in water just before chilling treatment or during chilling treatment and by interruption of chilling treatment with raising temperature to $20^{\circ}C$ for 20 days during chilling treatment. 7. The germination ratio of ginseng seeds which finished chilling treatment was highest at $10^{\circ}C$ and 62.5% was the estimated soil moisture for the best germination of ginseng seeds. The ginseng seeds were found to require high amount of oxygen for germination. 8. Only water soluble material in homogenized ginseng seeds showed a significant inhibiting effect on the seed germination of sesame, millet and soybean. Water soluble material dissolved from undehisced ginseng seeds showed stronger inhibiting effect on the seedling growth of sesame than material from dehisced ginseng seeds. Extraction temperature did not influence the inhibiting effect of the material dissolved from ginseng seeds on the seedling growth of sesame. 9. Water soluble materials dissolved from the berry pulps, leaves, fresh roots and dried roots also showed a significant inhibiting effect on the seedling growth of sesame. 10. Water soluble materials dissolved from the ginseng seeds, leaves and fresh roots showed a significant inhibiting effect on the germination of true fungi and the growth of spawn but the growth of phytopathogenic bacteria was not. 11. Among the water soluble materials dissolved from ginseng seeds, the materials of low molecular weight less than 3,000 were resulted a significant inhibiting effect on the seedling growth of sesame and the materials of high molecular weight also showed an inhibiting effect.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.97-104
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• 2006

This study was conducted to investigate effect of temperature and NaCl on the seed germination and water absorption of Thuja orientalis. Seeds were treated with 0, 500, 1,000, 2,000 and 4,000ppm of NaCl and placed in different chambers at 15, 20, 25 and $30^{\circ}C$, respectively. And seed properties and relative water absorptions were analyzed. Germination decreased with the increase of both temperature and NaCl concentration, and especially the difference was obvious at $30^{\circ}C$. Dormancy and mortality increased with the increase of temperature in non-NaCl treatment. Two-way analysis of variance showed significant effects of temperature, NaCl concentration and interaction between temperature and NaCl concentration (p<0.001). Mean germination time increased with the increase of NaCl concentration at 15, 20 and $25^{\circ}C$ but decreased at $30^{\circ}C$ because the seeds were mortal by NaCl high concentration, Germination speed and germination performance index decreased with the increase of NaCl concentration. Those represented decreasing tendency with NaCl concentration but high positive correlation with germination. Relative water absorption decreased with the increase of NaCl concentration and represented high values at $15^{\circ}C$, and showed high positive correlations with germination, germination speed and germination performance index. It was reported that the high temperature and salinity were inhibitive factors of seed germination of Thuja orientalis.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.31-35
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• 2010

Several experiments were conducted to investigate the germination characteristics among the Rumex crispus, R. japonicus, R. obtusifolius, and R. nipponicus. The germination point of R. japonicus was started 4 days after application, and then R. obtusifolius (5th), R. crispus (6th), R. nipponicus (10th) with appeared order. The optimum temperatures for germination of all tested Rumex spp. occurred at the $15^{\circ}C$. Especially, R. obtusifolius will have the widest germination range at $10^{\circ}C{\sim}30^{\circ}C$. To compared with 1996 and 2004 year selected R. japonicus germination were did not significantly difference, otherwise, all tested Rumex spp. on dark condition was not germinated. The parameters of germination characteristics of Rumex spp. were investigated mean germination rate (MGR, %), mean germination time (MGT, day), mean germination velocity (MGV, No./day), and coefficient of germination uniformity (CGU). The R. japonicus compared in tested Rumex spp. showed the highest MGR. The MGT and MGV of R. obtusifolius was appeared the earliest compared with R. obtusifolius and R. crispus.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.145-156
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• 1996

The effects of priming with different polyethylene glycol (PEG 6, 000) solutions on the germination, emergence characteristics and early plant growth in rice, barley and wheat were investigated. Rice, barley and wheat seeds were subjected to various priming conditions of osmotic potentials (-0. 75, -1.00 and -1.50 MPa) of PEG, and incubation period were 5 days at 25, 20, 2$0^{\circ}C$, respectively. The percentage of water absorption was highest in barely, and lowest in rice, and all the treatments enhanced water absorption in barley, but showed no significant effects in rice and wheat. Respiration quotient was lower than none PEG treatment seeds, and those of barley and wheat were higher than rice regardless of PEG concentration, and that of rice increased with high PEG concentration. Total germination percentage of osmoconditioning seeds with PEG was higher than that of none treatment seed, and those of barley and wheat were significant. The artificially deteriorated seeds with PEG treatment seeds after ageing treatment could recover to nearly the same germination level as that of the control seeds. The effects of coating polymer were higher than osmoconditioning with PEG, and germination characteristics in rice showed varietal difference at PVP and waterlock at recoated seeds after PEG treatment. Osmoconditioning with PEG reduced mean germination and emergence time, but there was no difference among PEG concentrations. The plant height of PEG treaed seed in rice was taller and those of barely and wheat showed varietal difference, and those of polymer-coated seed after PEG treatment were different among the polymers. The dry weight of PEG treatment were different among the crops, and those were increased with the high PEG concentration. The emergence percentage of PEG-treated seed were higher than none-treated seed, and those were decreased with the increased PEG concentration, and the highest emergence percentage of rice, barley and wheat were 90, 50 and 50% soil moisture content, respectively. The time to emergence in rice was longer than barley and wheat, and those in rice was shortened in high soil moisture content, and barley and wheat were shortened in low soil moisture content.

• Korean Journal of Environmental Agriculture
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• v.19 no.1
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• pp.58-61
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• 2000

This study was conducted to determine the effect of low dose ${\gamma}-ray$ on the germination rate and physiology of germinative seeds of welsh onion ( Allicm fistulosum L. cv. Sukchangwoidae ) and spinach ( Spinacia oleracea L. cv. Chungrok ). The germination rate of irradiation group was much higher than that of the control. Especially it was noticeably higher in 1 or 2 Gy irradiation groups in the sowing spinach seeds on paper towel. On the welsh onion, the germination rate of the 1 Gy irradiation group increased by 17% compared to that of the control. Ion leakage from seeds irradiated with low dose of ${\gamma}-ray$ was decreased compared to that from the control especially at the early stage of incubation when examined by means of electric conductance. This tendency was confirmed in seeds of welsh onion and spinach. Starch hydrolysis was stimulated by ${\gamma}-ray$ irradiation in welsh onion. Furthermore ${\gamma}-ray$ irradiation was beneficial to keeping the vitality of seeds as determined through decarboxylation of glutamic acid.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.50-50
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• 2019

본 연구는 국내 자생 국화과의 산국속 3종을 대상으로 국립백두대간수목원 시드뱅크 내 장기저장 가능성을 확인하고자 수행되었다. 국화과 3종의 종자는 경북 봉화군 춘양면에서 2017년 11월에 채종한 후 선별하였다. 종자는 저장조건별($-20^{\circ}C$, 40%RH /$5^{\circ}C$, 30%RH /$15^{\circ}C$, 15%RH) 12주간 저장하여 2주단위로 발아실험을 실시하였다. 발아실험은 광12시간으로 25/15(12/12h)$^{\circ}C$ 변온조건으로 진행하였다. 종자는 10립 3반복으로 1% Agar배지에 치상하여 유근이 1mm 이상 출현한 것을 발아립으로 정의하였다. 평균발아율(GP)과 발아속도(MGT)를 조사하였다. 구절초를 조건별 2주 저장 후 평균발아율과 평균발아일수를 조사한 결과 $-20^{\circ}C/5^{\circ}C/15^{\circ}C$에서 각각 100%, $4.0{\pm}0.09$일 / $96.7{\pm}3.33%$, $3.7{\pm}0.08$일 / $93.3{\pm}3.33%$, $3.6{\pm}0.11$일로 조사 되었으며 12주 저장 후 100%, $4.1{\pm}0.27$일 / $96.7{\pm}3.33%$, $4.4{\pm}0.21$일 / $96.7{\pm}3.33%$, $3.7{\pm}0.13$일로 조사되어 저장 후 발아율의 감소를 보이지 않았으며 $5^{\circ}C$ 저장에서 평균발아일수가 길어지는 경향을 보였다. 포천구절초의 경우 $-20^{\circ}C/5^{\circ}C/15^{\circ}C$에서 각각 $96.7{\pm}3.33%$, $4.4{\pm}0.36$일 / 100%, $4.2{\pm}0.09$일 / $93.3{\pm}6.67%$, $4.1{\pm}0.22$일로 조사 되었으며 12주 저장 후 $96.7{\pm}3.33%$, $4.2{\pm}0.30$일 / $96.7{\pm}3.33%$, $4.2{\pm}0.15$일 / 100%, $4.0{\pm}0.09$일로 조사 되어 저장 전 후 차이를 확인 할 수 없었다. 한라구절초의 경우 $-20^{\circ}C/5^{\circ}C/15^{\circ}C$에서 각각 $93.3{\pm}3.33%$, $3.4{\pm}0.10$일 / $93.3{\pm}3.33%$, $3.7{\pm}0.08$일 / $93.3{\pm}6.67%$, $3.8{\pm}0.02$일로 조사 되었으며 12주 저장 후 $96.7{\pm}3.33%$, $4.0{\pm}0.21$일 / $96.7{\pm}3.33%$, $4.2{\pm}0.03$일 / $96.7{\pm}3.33%$, $3.8{\pm}0.09$일로 조사되어 발아율이 소폭 상승하는 경향을 보였다. 산국속 3종의 종자는 12주 저장 후 기간 및 저장조건에 따른 평균발아율과 발아속도에서 유의적인 차이를 나타내지 않았다. 이상의 결과로 산국속 3종 구절초, 포천구절초, 한라구절초의 종자는 저장기간 및 조건에 대해 민감한 반응을 보이지 않아 장기저장이 가능한 진정종자(Orthodox seed)로 판단된다.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.274-279
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• 2011

In order to know the effect of seed size on the early plant life history, we measured the rate of germination, early growth and winter survival of four oak species with different acorn size (large, medium and small). The mean germination rate was higher in the larger seed species ($Q.$ $acutissima$ and ($Q.$ $variabilis$) than those of the smaller seed species ($Q.$ $aliena$ and $Q.$ $serrata$). Within $Q.$ $variabilis$, the germination rate decreased with larger acorn size class but that of rest other species was not significantly affected by the acorn size. The early mean growth rate decreased with acorn size in the order of $Q.$ $acutissima$, $Q.$ $variabilis$, $Q.$ $aliena$ and $Q.$ $serrata$. The early mean growth rate was higher in large or medium size than in small size of $Q.$ $acutissima$ and $Q.$ $variabilis$, but it showed no difference with acorn size in $Q.$ $aliena$ and $Q.$ $serrata$. Survival rate in winter was the highest in the medium acorn size among 4 oak species, the seedling of $Q.$ $serrata$ and $Q.$ $variabilis$ did not survive in both large and small acorn size. These results indicate that seed size influences a different effect on the early plant life history stage.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.169-174
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• 1997

This study was conducted to obtain basic information needed to develop the effective weed control method by using thermal weeder which uses LPG as fuel. For survey of weed distribution in herb field, weed species were investigated at two kinds of herb field of Codonopsis lanveolata and Aster scaber located in Hoengseongkun and Hwacheonkun, Kangwondo. In Codonopsis lanveolata field, Digitaria sanguinalis and Persicaria viridis L were dominated. In Aster scaber field, Digitaria sanguinalis and Erigeron canadensis were dominated. In general, Grasses weeds in herb were more dominated than other broadleaf and Cyperaceae weeds. The treatments of $H_2SO_4$ in weed seeds were more effective for germination of several weeds, compared with that nontreated weed seeds. However, the rate of germination differed depending on weed species and tereatment time of $H_2SO_4. Also, viability of weed seeds was significantly affected by irradiation time and temperature. As the temterature and treatment time of irradiation increased, the rate of germination was decreased.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.84-84
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• 2019

산채류의 종자 및 종묘는 시중 거래가격이 높게 형성되어 재배농가의 경영비 증가로 이어지고 있다. 특히 산채 꾸러미에 분류되는 작물 중 개미취(Aster tataricus), 섬쑥부쟁이(Aster glehnii ), 우산나물(Syneilesis palmata)은 주로 종묘의 형태로 공급되어 수급이 안정치 못하다. 이에 본시험은 개미취, 섬쑥부쟁이, 우산나물의 우량종자 생산을 위한 종자 결실률 향상 재배조건과 채종적기를 구명하고자 하였다. 시험작목을 전북 남원시 허브산채시험장에서 2016년 4월에 종묘를 노지에 정식하였다. 적정 채종 재배조건을 구명하기 위해 2018년 노지, 하우스, 55% 차광막을 설치한 처리구별 각 작물의 개화시기, 개화율, 생육특성, 결실률 등을 조사하였다. 또한 채종적기를 설정하기 위해서 각 작물별 개화 후 50일, 70일, 90일, 110일, 130일 간격으로 채종하여 결실률과 종자 발아율을 조사하였다. 개화가 시작된 일자는 개미취의 경우 하우스재배, 노지재배, 차광재배 순으로 빨랐으며 섬쑥부쟁이는 노지재배에서 개화시기가 가장 늦었다. 모든 작물의 개화가 종료되는 시점은 처리 간 차이가 없었다. 개화율은 개미취와 섬쑥부쟁이는 노지재배, 하우스재배, 차광재배 순으로 많았으며 우산나물은 노지재배시 개화율이 9.3%로 매우 낮았다. 개화기 생육특성을 조사한 결과 개미취는 초장과 엽폭이 차광재배, 하우스재배, 노지재배 순으로 커지는 경향이었으며, 섬쑥부쟁이는 하우스 재배에서 타 재배방법에 비해 우수하였다. 우산나물은 차광재배에서 생육이 가장 좋았다. 결실률은 개미취와 섬쑥부쟁이는 재배방법에 상관없이 결실률이 60%이상이었다. 반면 우산나물은 평균 31%로 타작물에 비해 상대적으로 결실률이 낮았으며 차광 재배일 때 43%로 가장 결실률이 좋았다. 개미취는 개화 50일 이후부터 종자 결실률이 65%이상으로 높았으며, 섬쑥부쟁이의 결실률은 개화 후 50일이 가장 좋았으며, 그 이후로 점차 결실률이 떨어지는 경향이었다. 우산나물은 개화 후 90~110일경에 가장 결실률이 높은 경향이었다. 개미취는 개화 후 70일일 때 가장 발아율이 낮았으며, 90일일 때 가장 발아율이 좋았다. 섬쑥부쟁이의 발아율은 개화 50일 이후부터 최종 채종시기까지 80%이상으로 우수하였다. 우산나물은 채종시기에 따라 발아율 정도가 매우 상이하여, 채종시기가 늦어질수록 발아율이 높아졌다. 따라서 개미취는 하우스 재배를 통해 개화 후 90일 때 채종하는 것이 가장 발아율이 좋았으며, 섬쑥부쟁이는 차광재배일 경우 개화 50일 이후에는 발아율이 90% 이상이므로 이때부터 종자비산 전까지가 채종적기로 판단된다. 우산나물은 타작물에 비해 다소 결실률이 떨어지나 차광재배 시 개화 110일 후 우량종자를 채종할 수 있을 것으로 여겨진다.