• Journal of Korean Society of Forest Science
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• v.89 no.2
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• pp.185-197
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• 2000

This study was carried out to research structure of forest vegetation and site condition, and supply basic data for conservation of pine forest and ecologically sustainable forest management and control in Anmyondo area. The forest of Anmyondo were largely classified as four forest community : Pinus densiflora community, Pinus thunbergii community, Pinus rigida community, and Quercus variabilis community. Organic matter, total nitrogen, and other nutrients in soil of the study area were relatively low, and average soil pH was 4.9. Soil texture was sandy clay or heavy clay and so the aeration and permeability could be poor. In this study site, the methods such as the promotion of germination of pine seeds through removal of forest floor and inducement of natural regeneration through such method as regeneration under shelter wood would be better than the method such as afforestation after clear-cutting for conservation the pine forests. Also, it will be a good method to practice tending for hardwood species in the dominant areas by hardwood species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.21-24
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• 2004

To determine lethal temperature of alfalfa (Medicago sativa L. cv Vernal) at heat-stressed conditions, seedlings grown in a small pots for 4 weeks were subjected to different temperature regimes of heat treatment. No apparent demage was observed when the plants were treated at 45, 50 or $60^{\circ}C$ for 1 h. Heat treatments at 60 and $65^{\circ}C$ for 1 h, several plants were withered and showed damage symptom on their leaves. When the plants were exposed to $70^{\circ}C$ for 1 h, most of leaves were severely withered, but it was not lethal conditions for the whole plants. By contrast, most of plants were died within one day after heat treatment at $80^{\circ}C$ for 1h. Furthermore, plants exposed to $80^{\circ}C$ for 50 min were also died within 7 days. It was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 45 min. These results indicate that heat treatment at $80^{\circ}C$ for 50 min is an optimum condition to distinguish the lethality of alfalfa plants. Simple viability assay system established in this study will be useful fer selection and characterization of heat-tolerant transgenic alfalfa plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.25-28
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• 2004

To determine lethal temperature of orchardgrass (Dactylis glomerata L. cv. Janbeol 102) developed in Korea at heat-stressed conditions, seedlings grown in a amall pots for 4 weeks were treated at $45^{\circ}C$, $50^{\circ}C$ or $55^{\circ}C$ for 1 h. Heat treatments at $60^{\circ}C$ and $65^{\circ}C$ for 1 h, several plants were withered and showed damage symptom on their leaves. When the plants were exposed to $70^{\circ}C$ for 1 h, most of leaves were severely withered, but it was not lethal conditions for the whole plants. By contrast, most of plants were died within one day after heat treatment at $80^{\circ}C$ for 1h. Furthermore, plants exposed to $80^{\circ}C$ for 55 min were also died within 7 days. It was found that new shoots were regenerated from the plants that had been treated at $80^{\circ}C$ within 50 min. These results indicate that heat treatment at $80^{\circ}C$ for 55 min is an optimum condition to distinguish the lethality of orchardgrass plants. Simple viability assay system established in this study will be useful for selection and characterization of heat-tolerant transgenic orchardgrass plants.

• Weed & Turfgrass Science
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• v.4 no.2
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• pp.118-123
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• 2015

Metagenomics is a powerful tool to isolate novel biocatalyst and biomolecules directly from the environmental DNA libraries. Since the metagenomics approach bypasses cultivation of microorganisms, un-cultured microorganisms that are majority of exists can be the richest reservoir for natural products discovery. To discover novel herbicidal substances from soil metagenome, we established three easy, simple and effective high throughput screening methods such as cucumber cotyledon leaf disc assay, microalgae assay and seed germination assay. Employing the methods, we isolated two active single clones (9-G1 and 9-G12) expressing herbicidal activity which whitened leaf discs, inhibited growth of microalgae and inhibited root growth of germinated Arabidopsis seeds. Spraying butanol fraction of the isolated active clones' culture broth led to growth retardation or desiccation of Digitalia sanguinalis (L) Scop. in vivo. These results represent that the screening methods established in this study are useful to screen herbicidal substances from metagenome libraries. Further identifying molecular structure of the herbicidal active substances and analyzing gene clusters encoding synthesis systems for the active substances are in progress.

• Korean Journal of Medicinal Crop Science
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• v.24 no.2
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• pp.101-109
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• 2016

Background: The purpose of this study was to evaluate methods to reduce seeding expenses, thereby increasing farm income. This study investigated the effects of rain shelter controlled cultivation and adapted seeding times on the stable seed production of Scutellaria baicalensis Georgi. Methods and Results: Seed germination was conducted under 10 condition compose to control, water washing, cold storage at $4^{\circ}C$ for 15 days, seed sterilization with a benomyl pesticides, hormone treated seed by submerging in 100 ppm $GA_3$ with the cold storage at $20^{\circ}C$ and $25^{\circ}C$, darkness in a covered petridish and illuminated with a 1,500 Lux lamp. There were three cultivation type, open cultivation with non-woven fabric mulching, cultivation with a vinyl covering and rain sheltered in a plastic greenhouse. Sowing dates were April 27, May 18, June 7 and June 28, 2013. Plants were spaced 10 cm apart in rows 30 cm apart. Mixed oil cake fertilizer, $N-P_2O_5-K_2O$ (12-10-10) was applied at $600kg{\cdot}10a^{-1}$. Conclusions: Optimum germination occured in darkness at $25^{\circ}C$ and cold storage after submerging in $GA_3$. The highest seed yields ($4.5kg{\cdot}10a^{-1}$) occurred in the plastic greenhouse for the April 25 sowing. The highest root yield (17%) was found on April 1, under greenhouse conditions.

• Weed & Turfgrass Science
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• v.1 no.4
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• pp.44-49
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• 2012

The objective of this study was to know the herbicidal activity of the essential oil from amyris (Amyris balsamifera). In a seed bioassay experiment, the amyris essential oil inhibited the growth of rapeseed (Brassica napus) by fifty percent at 8.8 ${\mu}g\;g^{-1}$. And in a greenhouse experiment, sorghum, barnyard grass and Indian jointvetch, which was applied in above-ground parts, with the amyris essential oil at 4,000 ${\mu}g\;ml^{-1}$ showed visual injuries of 90, 70, and 70, respectively (0, no damage; 100, total damage). However, soil application of the essential oil did not show such herbicidal injuries. In a field experiment, foliar application of the amyris essential oil at 5% controlled effectively weeds such as barnyardgrass, shepherd's purse, and clover in 24 hours. Our results indicated that the amyris essential oil had herbicidal activity. To understand the composition of the amyris essential oil, the oil was analyzed by gas chromatography-mass spectometry with solid-phase micro-extraction apparatus. There were 15 organic chemicals in the oil and the major constituents were calarene, elemol, ${\gamma}$-eudesmol, curcumene, ${\beta}$-sesquiphellandrene, zingiberene, selina-3,7(11)-diene, 1,3-diisopropenyl-6-methyl-cyclohexene, ${\beta}$-bisabolene, and ${\beta}$-maaliene. Overall results suggest that the amyris essential oil had a herbicidal activity with fast, contact, and non-selective mechanism.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.595-598
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• 2009

A new yellow gerbera (Gerbera hybrida Hort.) cultivar "Golden Time" was developed from a cross between '99-ga-2' and '99-na-7-6', followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2004 to 2007. "Golden Time" has semi-double type large flowers in a good harmony with yellow (RHS, 17-B) ray floret and brown center. It has good, stable flower shape and strong peduncles, and its vase life was 12.3 days. The average flower yield of "Golden Time" was about 49.5 stems per plant/year in greenhouse trails during 2004 and 2007. This cultivar was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009. Year-round production of this cultivar is possible in the greenhouse condition throughout the Korean climate.

• Korean Journal of Breeding Science
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• v.40 no.3
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• pp.278-285
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• 2008

An efficient transformation method for soybean [Glycine max (L.) Merr.] using meristematic tissues of germinating seeds has been established. The embryonic axes were excised from germinating seeds of Korean soybean cultivar, Iksannamulkong and 0.5-2 cm long segment containing meristematic tissues were prepared by cutting hypocotyl region. The explants were inoculated with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector with the bar gene as a selectable marker gene and a ${\beta}-glucuronidase$ (GUSINT) reporter gene, and then co-cultured for 7 days on co-cultivation medium (CCM). The meristematic tissues were cultured on shoot induction medium (SIMP6) supplemented with 0.4 mg/l $N_6-benzylaminopurine$ (BAP) and 0.1 mg/l indolebutyric acid (IBA) in the presence of 6 mg/l L-phosphinotricin (PPT) for 2 weeks and the surviving explants were transferred to shoot elongation medium (SEMP6). Transformation was confirmed by Southern blot analysis and the transformation efficiencies ranged from 1.48 to 2.07%. The new modified transformation method was successfully implemented for obtaining several transgenic lines with SMV-CP gene. It is expected that this method could efficiently be used for the transformation of recalcitrant soybean cultivars.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.284-290
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• 2010

This study was carried out to breed a self-compatible variety of Tiger Lily from the seedlings originated from Chinju city, Gyeongsangnam-do. The morphological characteristics, ploidy, and the resistance of seedlings to virus infection were investigated. A progeny test was also conducted to examine whether the propagated progenies had the same characteristics as the mother plant. The self-compatible diploid lily variety developed in this study showed a tall type like native triploid lily, Lilium tigrinum, and bulbils were formed on both lily varieties. The morphological characteristics of the flowers and leaves were not much different between the varieties, while the size of the bulbils and bulbs showed significant differences. The percentage of seed generation by self-pollination was 72.6% for the self-compatible variety, while there was no seed generated for native triploid lily. The number of chromosome was 2n = 26 (x = 13, diploid) for the self-compatible variety, while 2n = 39 (x = 13, triploid) in native lily variety. The progenies of the self-compatible diploid lily variety showed the same characteristics as those of its mother plant in morphology, seed germination, and polyploidy. The mother plant of the self-compatible diploid lily variety showed 58% pollen germination and the 2-year-old and 3-year-old progenies showed similar germination percentages. The pollen grains of Korean native triploid lily, however, never germinated.

• FLOWER RESEARCH JOURNAL
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• v.16 no.2
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• pp.124-127
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• 2008

A new orange-colored, cut flower gerbera (Gerbera hybrida Hort.) cultivar 'Sweety' was developed from a cross between 'Grandeur' and 'Songsongee', followed by selections of seedlings and lines at the Flower Breeding Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) from 1999 to 2004. Three time evaluations were conducted from 2003 for the detailed characteristics of the new cultivar. 'Sweety' has semi-double type mini- or medium-sized flowers in good harmony with orange(RHS, 38-A) ray florets and a green center. It has good, stable flower shape and strong peduncle, and its vase life was 10.4 days. The average flower yield was about 51.0 stems per plant/year in greenhouse trails during 2003 and 2004. This cultivar was registered to the Korea Seed and Variety Service for commercialization in 2006. Year-round production of this cultivar is possible in the greenhouse condition in Korean climate.