• Title/Summary/Keyword: 종묘

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Development and growth in fertilized eggs and larvae of the Japanese geoduck, Panopea japonica reared in the laboratory (코끼리조개, Panopea japonica (A. Adams)의 수정란 발생과 유생 성장)

  • Nam, Myung-Mo;Lee, Chu;Kim, MeeKyung;Kim, Jae Won;Kim, Young Dae
    • The Korean Journal of Malacology
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    • v.30 no.4
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    • pp.303-309
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    • 2014
  • The development of Japanese geoduck, Panopea japonica, grown under culture conditions, has been examined through the morphological characteristics in fertilized egg, larvae and juvenile. Gametes were stripped from ripe broodstock and placed into two separate containers. Eggs were washed through a $40{\mu}m$ sieve and fertilized with dilute sperm solution. Developing larvae were maintained at $19{\pm}1^{\circ}C$. Fertilized eggs with $81.6{\mu}m$ diameter developed to trochophores within 14 h and to D-stage larvae ($116{\mu}m$ shell length) within 27 h. Larvae were spontaneously settled at shell length of $311{\mu}m$ after 20 days. The hatching from fertilized eggs and larval rearing were normally available in $18.5-21.5^{\circ}C$, and the growth was good in a cashmilon substrate, as well as sand. After rearing of day 108 from metamorphosis, the shell length of juvenile P. japonica reached 13 mm, and growth rate of shell length of the juvenile was $117.5{\mu}m/d$.

The Effect of Neuroactive Compounds on Settlement of Pacific Oyster, Crassostrea gigas Pediveliger Larvae (굴, Crassostrea gigas 부착기 유생의 부착에 미치는 신경전달물질종의 영향)

  • Hur, Youngbaek;Jo, Qtae;Byun, Soongyu;Mun, Tesek
    • The Korean Journal of Malacology
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    • v.30 no.4
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    • pp.343-351
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    • 2014
  • We determined the effects of neuroactive compounds known as synthetic larval settlement inducers on the settlement of the Pacific oyster C. gigas pediveliger on the larval collector. Six types of the inducers, serotonin (5-HT), ${\gamma}$-amino butyric acid (GABA), L-3,4-dihydroxyphenylalanine (L-DOPA), norepinephrine, epinephrine and methyl bromide (MB) were tested. All the chemicals induced larval settlement, MB being the most effective with settlement rate of $42.7{\pm}2.7%$, followed by GABA ($35.4{\pm}2.0%$), 5-HT ($29.1{\pm}2.2%$), L-DOPA ($19.2{\pm}2.1%$), epinephrine ($15.2{\pm}0.9%$), and norepinephrine ($11.0{\pm}1.2%$). The chemicals ${\gamma}$-amino butyric acid and methyl bromide were also better in terms of settled density on the collector with their respective density of $1.97{\pm}1.42$ and $2.37{\pm}1.86ind/cm^2$, reminiscent of being most effective candidates for a larval settlement inducer in the oyster hatchery.

Growth characteristic of lettuce (Lactuca sativa L.) based on Pulse Width modulation of artificial light sources with UV-A. (UV광원을 포함한 인공광원의 펄스폭 변조에 따른 적치마 상추의 생육특성)

  • Kwak, Su-Ji;Kwak, Eun-Ji;Lee, Hyun-Hee;Lee, Seung-Ki;Han, Jae-Woong;Jeon, Myung-Jin;Kim, Woong
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2017.04a
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    • pp.123-123
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    • 2017
  • 식물공장 내 작물의 생육은 제공되는 인공광원의 광원 및 광질에 영향을 받으며, 광흡수 파장에 따라 다르다. 또한 광합성에 효과적인 형태의 빛의 계속적인 빛의 조사보다는 광 펄스를 조절하여 공급하여 준다면 더욱더 효과적인 생육 환경을 제공해 줄 수 있다. 식물의 최적 생장을 위해 특정 파장대의 빛을 선택적으로 조사할 수 있는 LED특성을 사용하여 UV광원을 포함한 인공광원의 펄스폭 변조에 따른 적치마 상추의 생육특성을 알아보고자 하였다. 광환경은 Red(660 nm), Blue(450 nm), UV(395 nm) LED를 8:1:1 비율로 광량 $160{\mu}mol{\cdot}m^2{\cdot}s^{-1}$, 주파수 1.25, 2.5, 3.75, 5.0 kHz로 조사하여 주었으며, 온도 $20{\sim}23^{\circ}C$, 습도 50~60%, $CO_2$농도 1,000 ppm으로 조성하여 주었다. 아시아종묘 적치마상추를 파종 후 18일 째 되는날 정식, 정식 후 14일 28일 째 되는날 SPAD, 지상부 지하부의 생체중 및 건물중, 엽폭, 엽장을 측정하였으며, 측정한 엽폭과 엽장을 이용하여 엽형지수 산출, 지상부 지하부 생체중 값을 이용하여 S/R율을 산출하였다. SPAD 측정결과 생육시기가 증가할수록 SPAD함량은 감소하였으며, 1.25와 2.5 kHz에서 생육 시기 증가에 대한 SPAD함량 감소가 컸고, 3.75와 5.0 kHz의 경우 SPAD함량의 감소량은 작았다. 지상부 생체중은 3.75 kHz에서 121.51 g으로 가장 높은 값을 나타냈으며, 2.5, 1.75, 5.0 kHz 순으로 높은 값을 나타났다. 지하부 생체중의 경우 3.75 kHz에서 31.31 g으로 가장 높은 값을 나타냈으며 2.5, 5.0, 1.25 kHz 순으로 높게 나타났다. 엽형지수는 생육 시기가 증가에 따라 감소하는 것으로 나타났으며, 1.75, 2.5, 5.0, 3.75 kHz 순으로 크게 나타났다. 지상부 건물중 측정결과는 지상부 생체중 결과와, 지하부 건물중의 측정결과는 지하부 생체중 결과와 동일하였다. S/R율은 1.75 kHz를 제외하고 생육시기가 증가할수록 S/R율은 감소하는 것으로 나타났으며, 주파수가 높아질수록 S/R율은 감소하는 것으로 나타났다. 엽형지수는 엽폭/엽장으로 엽형지수를 산출한 결과로 값이 작을수록 엽폭이 넓은 형태를 의미하며, 생체중값이 가장 크게 나타났던 3.75 kHz에서 엽형지수의 값이 가장 낮게 나타났으며 3.75 kHz를 제외하고 주파수가 높을수록 엽형지수의 값이 낮게 나타났다. 이에 주파수에 따른 적상추의 생육은 3.75 kHz에서 가장 좋은 것으로 판단할 수 있었다.

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Effect of Water Temperature Condition on Growth and Survival of Juvenile Geoduck (Panopea japonica A. Adams, 1850) (사육 수온이 코끼리조개 Panopea japonica (A. Adams, 1850) 치패의 성장과 생존에 미치는 영향)

  • Nam, Myung-Mo;Lee, JooYoung;Lee, Chu;Kang, Hee Woong;Kim, Young Dae;Byun, Soon-Gyu;Yoo, Hae-Kyun
    • The Korean Journal of Malacology
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    • v.31 no.4
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    • pp.263-266
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    • 2015
  • This study was performed to determine the effect of water temperature condition on survival rate and growth of juvenile geoduck (Panopea japonica). Panopea japonica juveniles were bred for 6 weeks at 12, 15, 18, 21, 24 and $27^{\circ}C$ on incubator. Juveniles median lethal times (LT50) were 29 hours in $27^{\circ}C$, 14.5 day in $24^{\circ}C$, 37.4 day in $21^{\circ}C$. Survival rate of water temperature 12, 15 and $18^{\circ}C$ showed a high survival rate in 95.0%, 95.0% and 93.3% at 6 weeks. As a result of culturing for 6 weeks at 12, 15, 18, 21 and $24^{\circ}C$, a daily growth of shell length and total weight was 0.10 mm, 0.12 mm, 0.13 mm, 0.16 mm and 0.14 mm, and 2.21 mg, 2.65 mg, 2.84 mg, 3.13 mg and 2.93 mg. Juvenile shell length and total weight have significantly increased at $15-21^{\circ}C$. However, mortality rate has significantly increased at more than $21^{\circ}C$. As a result, appropriate water temperature for juveniles culturing considered $15-18^{\circ}C$.

Effects of Media and Plant Growth Regulators on Germination of Somatic Embryos of Aralia elata Seem (두릅나무 체세포배(體細胞胚)의 발아(發芽)에 미치는 배지(培地) 및 식물생장조절제(植物生長調節劑)의 영향(影響))

  • Park, Cheol-Ho;Lee, Youn-Su;Jhang, Han-Ho;Kim, Nam-Soo;Shin, Young-Boum
    • Korean Journal of Medicinal Crop Science
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    • v.2 no.3
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    • pp.241-245
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    • 1994
  • Effects of media and plant growth regulators on the germination of somatic embryos of Angelica tree(Aralia elata Seem.) was studied for the mass production of Angelica tree through tissue culture. MS medium was found to be the most effective for the germination of somatic embryos(65% germination rate), Among the MS medium, the medium containing 25% less inorganic salts and 1% less sucrose was found to be the most effective. Gelling agent with 0.2-0.3% gelrite promoted the germination of somatic embryo$(65{\sim}70%)$ and caused good growth of shoots and roots. 0.1 mg/l of BA and kinetin treatment caused $65{\sim}70%$ germination rate of somatic embryos and good growth of shoots and roots, and resulted in high percentage of dry matter. 1mg/l or 5 mg/l treatment of putrescine, and 10 mg/l treatment of spermidine caused 90% germination rate of somatic embryos and good growth of plant organs, and inhibited vitrification of regenerated plants.

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Feeding and Digestion by Postlarvae and Juveniles of the Flounder, Paralichthys olivaceus (넙치, Paralichthys olivaceus 자어 및 치어의 섭식과 소화)

  • WON Moon Seong;CHANG Young Jin;YOO Sung Kyoo
    • Journal of Aquaculture
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    • v.1 no.1
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    • pp.1-11
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    • 1988
  • There are many problems to be solved for the establishment of more feasible and simplified method of seed production of marine fishes. One of the most important tasks in seed production is to clarify the feeding ecology of larvae and juveniles under. rearing conditions. In the present study, two groups of postlarvae-juveniles of the flounder, Paralichthys olivaceus were used. One ranging 11.38 to 17.08 mm in mean total length was fed with Artemia nauplii at two different water temperatures (19 to $21^{\circ}C$ and 24 to $25^{\circ}C$) and the other ranging 4.39 to 10.64 cm in mean total length was fed with prepared diet. Time required from the start of feeding to satiation was 45 to 55 minutes at 24 to $25^{\circ}C$ and nearly one hour at 19 to $21^{\circ}C$. Mean numbers of Artemia nauplii in digestive tract were 236 to 375 individuals per fish at 24 to $25^{\circ}C$. The amount of food remained in the intestine was $24.8\%$ of the total food in the digestive tract. In juveniles of 4.39 to 10.64 cm in mean total length, amount of prepared diet consumed at satiation was 0.04 to 0.46 g per fish. Digestive tract index in juveniles was rapidly decreased for 24 hours after satiation and kept nearly constant after 48 hours, showing that the time to completely digest the prepared diet in juveniles was estimated to be about 48 hours.

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Biological Studies on Aquaculture for Resources Enhancement of Protothaca jedoensis I. Egg Development and Larva Reared (살조개, Protothaca jedoensis 자원조성을 위한 양식생물학적 연구 I. 난발생과 유생사육)

  • Yoon, Ho-Seop;Kim, Jung;Choi, Sang-Duk
    • Journal of Aquaculture
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    • v.18 no.4
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    • pp.260-266
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    • 2005
  • In order to obtain the aquaculture fundamental data far resources enhancelnent of the Protothaca jedoensis, the egg development and larva growth were investigated at different conditions such as water temperature, phytoplankton and density. Water temperature, at which P. jedoensis egg successfully completed development, ranged from $15{\~}30^{\circ}C$. The required time from fertilization to D-shaped larva was 39.7 hours at $15^{\circ}C$, 31.2 hours at $20^{\circ}C$, 26.8 hours at $25^{\circ}C$ and 26.2 hours at $30^{\circ}C$ P. jedoensis. In regard to water temporature, growth and survival rates of larvae were high at $30^{\circ}C$. In growth and survival rates of larvae with various rearing densities, the highest aver-age growth and survival rates were 4${\~}$6 ind./ml When larvae were fed mixed phytoplankton, such as Isochrysis galbana, Pavlova lutheri and Chaetoceros calcitrans, their growth and survival rates were the high among the groups. In growth and survival rates of larvae with various rearing food concentrations, the highest average growth and survival rates were $218{\mu}m$, and $45\%$ at the food concentration of $1{\times}10^4$ cells/ml, respectively.

Size and Resting Egg Formation of Korean Rotifer, Brachionus plicatilis and B. calyciflorus (한국산 Rotifer, Brachinus plicatilis와 B. calyciflorus의 크기 및 내구란 형성)

  • Hur, Sung-Bum;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.9 no.3
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    • pp.187-194
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    • 1996
  • Sixteen strains of marine rotifer, Braohionus plicatilis were isolated from salt pond, estuary and lagoon. Among 16 strains, 2 strains were large (L)-type and the others were small (S) or ultra small (US)-type. Four strains of fresh water rotifer, B. calyciflorus were isolated from commercial fish ponds. The size of lorica and resting egg were measured. In B. plicatilis, the range of lorica length from S-type and S-type were $244.3{\~}255.3\;{\mu}m$ and $131.0{\~}165.8\;{\mu}m$, respectively. The major axis of resting egg in the marine rotifer were $93.7\~116.4\;{\mu}m$ for S-type and $142.4{\~}145.5\;{mu}m$ for L-type, respectively. In freshwater rotifer, B. calyciflorus, the size range of lorica and major axis of resting egg were $211.8\~229.9\;{\mu}m$ and $126.8\~140.2\;{mu}m$, respectively. The size of freshwater rotifer was larger than that of S-type marine rotifer, but smaller than that of L-type one. Growth and formation of resting egg of B. plicatilis were different among the strains. The maximum density of S-type and L-type rotifer was 753.3 inds./ml for H-S strain and 220 inds./ml for O-L strain, respectively. The largest production of resting egg of S-type and L-type rotifer were 86.7 inds./ml for YY-S strain and 45.8 inds./ml for O-L strain, respectively.

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Induced Ovulation in Catfish (Silurus asotus) by GnRH-Analogue (생식소자극호르몬방출호르몬 유사물질에 의한 메기(Silurus asotus)의 배란유도)

  • Kwon Hyuk-Chu;Choi Nack-Joong;Park Hong-Yang
    • Journal of Aquaculture
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    • v.9 no.3
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    • pp.205-213
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    • 1996
  • Experiments were carried out to investigate the effect of GnRH-analogue (GnRH-a) on the induction of ovulation in catfish, S. asotus. Fully matured female catfish ($250\~600\;g$) received a single intraperitoneal injection of GnRH-a ($50\~200\;{\mu}g/kg{\cdot}body$ weight) showed the successful induction of ovulation. More than $86\%$ of treated females were ovulated after injection of GnRH-a ($90\;{\mu}g/kg$) at $25{\pm}1^{\circ}C$. The majority of spawning took place within 22 to 25 hours after the injection. The gonadosomatic index (GSI) and pseudo-GSI in the group treated with $120\;{\mu}g/kg$ GnRH-a were $23-30\%$ and $18-21\%$, respectively. Average fertilization and hatching rates were $94\%\;and\;81\%$, respectively. Electron microscopically, gonadotrophs of maturing female catfish were characterized by the presence of numerous small, electron-dense granules of approximately $150\~300$ nm in diameter and a few larger, less electron-dense granules of approximately $800\~1000$ nm in size in their cytoplasm. Gonadotrophs of GnRH-a treated catfish showed that their was a distinct decrease in number of small and large granules. The rough endoplasmic reticulum was composed of numerous cisternae conspicuosly dilated to various degrees.

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Allozyme Variation of Pinus rigida Mill. in an F1-Hybrid Seed Orchard and Estimation of the Proportion of F1-Hybrid Seeds by Allozyme Analysis (잡종(雜種) 채종원(採種園)에서 리기다소나무의 Allozyme 변이(變異)와 Allozyme 분석(分析)에 의(依)한 잡종종자(雜種種字) 발생률(發生率)의 추정(推定))

  • Chung, Min Sup
    • Journal of Korean Society of Forest Science
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    • v.66 no.1
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    • pp.109-117
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    • 1984
  • Allozyme study for open pollinated seeds of forty nine pitch pine families in an $F_1$-hybrid seed orchard demonstrated that allozyme variants in aspartate aminotransferase(AAT), glutamate dehydrogenase(GDH) and leucine aminopeptidase(LAP) systems are encoded by at Least eight loci; five for AAT, one for GDH and two fur LAP. Allozyme variations showed polymorphisms at seven of the eight loci, except GDH. Average number of alleles examined over six loci were 2.33 and 2.67 for maternal and progeny groups, respectively. Average heterozygosity and genetic diversity computed over six loci were, respectively, 0.235 and 5.409 for maternal tree group, 0.238 and 5.569 for progeny group. The proportion of $F_1$-hybrid seeds estimated by allozyme analysis was 0.77%. The estimated proportion of $F_1$ hybrid seeds by allozyme study is in good agreement with the value 0.73% estimated by morphological study for the proportion of pitch ${\times}$ Loblolly $F_1$ hybrid seedlings at a nursery. Indications for Wahlund effect, high levels of self-fertilization and for non-random matings in the $F_1$ hybrid seed orchard call for cautions in estimating allele frequency changes and mating probabilities for the parental and progeny groups.

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