• Title/Summary/Keyword: 존재양식

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Purificatio and Characterization of Yolk Protein in an Abalone (Haliotis discus hannai) (참전복 (Haliotis discus hannai)의 난황단백질 분리와 특성)

  • 정태항;한명숙;김대중;임상구;김명희;한창희
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.271-278
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    • 1998
  • To clarify characteristics of yolk protein of abalone, yolk protein was purified from the ovarian egg extracts of mature female Haliotis discus hannai by a gel chromatography of sepharose CL-4B. From the results of immuno-electrophoresis and Ouchterlony's diffusion test to male and female sera and ovarian egg extracts using antibodies raised against mature female and male sera and male sera and ovarian egg extrascts, it was identified that the mature female serum had female specific serum protein and its antigenecity shared with ovarian egg extracts. A single type of yolk protein was purified from ovarian egg extracts, and it was composed of two subunits. Their molecular weights were estimated to be approximately 166 KDa and 113 KDa by SDSPAGE. The antiserum against yolk proteins cross-reacted with a mature female specific serum protein and extracts of hepatopancreas of vitellogeing females, but did not reacted with extracts of hepatopancreas of mature male.

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Science News Frame: A Study of Longitudinal Framing Analysis for Biotechnology (과학뉴스(Science News)연구: 생명공학 뉴스의 장기적인 보도경향연구)

  • Kweon, Sang-Hee
    • Korean journal of communication and information
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    • v.32
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    • pp.7-48
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    • 2006
  • The study explores how Korea's major newspaper cover about science news, especially how newspaper frame biotechnology news including new source, news construction ways, coverage trend. The research has a research design to find out coverage pattern or model with frame theory. The result shows that the newspaper has some aspect of frame through out the biotechnology development in the section, theme, source, complexity. The section has been expend to the society and international section, while the theme shift from disease or cancer cure to life itself, genome, or stem cell. In the complexity, the biotechnology news stories have been developed a story plot (event-problem-development-solution). In the climax, the news coverage focuses on the explanation of biotechnology news.

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Feeding of Bacteria by Copepod Tigriopus japonicus (Tigripus japonicus의 세균 섭이)

  • Jung, Min-Min;Rho, Sum;Kim, Pil-Yun
    • Journal of Aquaculture
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    • v.11 no.1
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    • pp.113-118
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    • 1998
  • This study was carried out to investigate feeding of bacteria by Tigripus japonicus (Copepoda : Harpacticoida) under axenic culture. The ovigerous females and nauplii were grown with feed of aquatic bacteria. Growth of RT bacteria strain was suppressed by feeding of co-existing T. japonicus. T. japonicus of non-axenic culture was observed with oil bead in the egg sac. On the other hand, early nhauplius stage did not develop to the next stage without stage took bacteria as food. And the adult of T. japonicus may utilize the baxteria as nutrient source for egg development.

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Reliability-based Design Criteria for Reinforced Concrete Structures Based on Partial and Combined Resistance Factor Formats (부분(部分) 및 조합저항계수형식(組合抵抗係數形式)에 의한 철근(鐵筋)콘크리트구조(構造)의 신뢰성(信賴性) 계기준(計基準))

  • Oh, Byung Hwan;Lee, Seong Lo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.10 no.1
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    • pp.87-97
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    • 1990
  • There is the need to balance safety, economy and serviceability in all phases of society problems. This is especially true in structural code formulation. where a framework is established by which practicing structural engineers can be assured of designing structures that reasonably meet the above three objectives. The existing design codes, which are generally based on the structural theory and certain engineering experience, do not realistically consider the uncertainties of loads and resistances and the basic reliability concepts. The purpose of the present study is therefore to develop the realistic reliability-based design criteria to secure adequate safety arid reliability, and to derive the models for partial and combined resistance factor formats. To this end, the reliability levels of our existing design code for concrete structures are first evaluated and the target reliabilities are determined, the new code formats are evolved from these target reliabilities. The present study indicates that the proposed formats exhibit relatively-uniform reliability and reasonably take into account the different material characteristics of concrete and steel in concrete structures.

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Effect of EM-fermented Orange in Commercial Diet on Growth of Juvenile Flounder, Paralichthys olivaceus (상품 사료에 첨가한 감귤발효액이 치어기 넙치, Paralichthys olivaceus의 성장에 미치는 영향)

  • 송영보;문상욱;김세재;이영돈
    • Journal of Aquaculture
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    • v.15 no.2
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    • pp.103-110
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    • 2002
  • Diets containing 0.02 to 10.0% EM-fermented orange (EFO) were fed to Paralichthys olivaceus(8.5 g), which were reared in flow-through system for 16 weeks. Groups fed on diets containing 0.1 and 0.2% EFO grew significantly faster; their feed coefficient and daily feeding rate were also higher. The number of goblet cells present in the mid-intestine of the fish receiving dietary EFO was significantly more. Total cholesterol level in plasma of the fish fed with 0.02% EFO was lower. However, there was no significant difference in GOT and GPT among the groups receiving different levels of EFO.

Genomic Characterization and Pathogenicity of Iridovirus Isolated from Pearl Gourami (Trichogaster leeri) (Pearl Gourami (Trichogaster leeri)로부터 분리한 Iridovirus의 유전적 특성과 병원성 분석)

  • Kim, Ho Yeoul;Jeong, Joon Bum;Jun, Lyu Jin;Yoon, So Hye;Cho, Hye Jin;Jeong, Hyun Do
    • Journal of Marine Bioscience and Biotechnology
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    • v.1 no.3
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    • pp.163-169
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    • 2006
  • In the survey of the samples infected by iridovirus, we found the natural outbreak of iridovirus disease in the ornamental fish, pearl gourami (Trichogaster leeri) in Korea. It was characterized by the appearance of enlarged cells and necrosis in the observation of the imprinted spleen cells under iridovirus. To determine the infection of iridovirus more accurately, PCR using 2 different primer sets for MCP and ATPase gene used for the diagnosis of iridovirus infection was done and found the produced fragments matched with the size of expectation. Partially determined nucleotide sequences of the MCP gene of the iridovirus isolated in this study showed very high homology (99.6%) with that of ISKNV as a reference strain. In challenge experiment with the iridovirus isolated from the moribund of pearl gourami, the pathogenicity of the isolated iridovirus was confirmed and suggested the potential of the risk associated the transfer of iridovirus from the ornamental fishes to the marine culturing fishes.

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Annual Reproductive Cycle of Korean Yellow Croaker Larimichthys polyactis (자연산 수컷 참조기 Larimichthys polyactis의 생식년 주기)

  • Kang, Duk-Young;Cho, Kee-Chae;Lee, Jin-Ho;Kang, Hee-Woong;Kim, Gyu-Hee;Kim, Hyo-Chan
    • Journal of Aquaculture
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    • v.22 no.1
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    • pp.5-10
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    • 2009
  • Spermatogenesis in male yellow croaker Larimichthys polyactis was histologically investigated by sampling testicular tissue from $2{\sim}3$ years old wild fishes captured from the coast of Mok-Po, South Korea. Spermatogenesis was characterized histologically, and staged according to the most advanced type of germ cell present. Annual reproductive cycle was classified into the following successive 4 stages: spermatogonia from August to September (rest stage), spermatogonia and spermatocytes from October to December (growth stage), spermatogonia, spermatocytes and spermatids from January to February (maturation stage), spermatogonia, spermatocytes, spermatids and spermatozoa from March to May (spermiation stage IV), and regressing testis from June to July (degeneration stage).

A Measurement Study of User Behavior and File Pollution in DHT-based P2P Networks (DHT 기반의 P2P 네트워크에서 사용자 행동양식 및 파일 오염에 관한 측정 연구)

  • Shin, Kyu-Yong;Yoo, Jin-Cheol;Lee, Jong-Deog
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.3
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    • pp.131-140
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    • 2011
  • File pollution (i.e., sharing of corrupted files, or contaminating index information with bogus index records) is a de facto problem in many file sharing Peer-to-Peer (P2P) systems in use today. Since pollution squanders network resources and frustrates users with unprofitable downloads (due to corrupted files) and unproductive download trials (due to bogus index records), the viability of P2P systems (and similar distributed information-sharing applications) is questionable unless properly addressed. Thus, developing effective anti-pollution mechanisms is an immediate problem in this literature. This paper provides useful information and deep insight with future researchers who want to design an effective anti-pollution mechanism throughout an extensive measurement study of user behavior and file pollution in a representative DHT-based P2P system, the Kad network.

Morphological Characteristics of Growth of Rice and Barnyardgrass under Various Cropping Patterns - II. Difference in Anatomical Characteristics (재배양식(栽培樣式)에 따른 벼와 피의 생장(生長) 및 해부형택학적(解剖形態學的) 차이(差異) - II. 재배양식(栽培樣式)에 따른 벼와 피간(間)의 해부학적(解剖學的) 차이(差異))

  • Chon, S.U.;Guh, J.O.;Park, S.J.
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.128-143
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    • 1994
  • At 5 DAS/T, leaf primordia of rice stems that were grown under dry condition in transverse sections were strongly stained while those under water condition had many aerenchyma cells well developed. On the other hand, leaf primordia and large air spaces in stem of transplanted rice were well developed. Rice in leaf anatomy had small and fine epidermal cells, chlorophyllous mesophylls, and bulliform cells but had no chlorophyllous vascular bundle sheath cells, while barnyardgrass leaf had large, rough and irregularly arranged epidermal cells, chlorophyllous vascular bundle sheath cells, and non-bulliform cells but had no chlorophyllous mesophylls. Epidermal cells of transplanted rice, however, were well developed, differentiated and sclerified. Cross sections of rice root under dry condition showed cell contents, regularly arranged cells, non-intercellular spaces and non-aerenchyma while under water condition had well-developed intercellular spaces, aerenchyma cells, small and densely arranged epidermis, sclerified exodermis and sclerenchyma cells. But root anatomy of transplanted rice consisted of finely, regularly arranged epidermis, well-developed intercellular spaces and nucleous cells.

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Effect of Continuous Cultivation Years on Soil Properties, Weed Occurrence, and Rice Yield in No-tillage Machine Transplanting and Direct Dry-seeding Culture of Rice (벼 무경운 기계이앙 및 건답직파 연속재배년수가 토양특성, 잡초발생 및 벼 생육에 미치는 영향)

  • Park, Hong-Kyu;Kim, Sang-Su;Choi, Won-Yong;Lee, Ki-Sang;Lee, Jae-Kil
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.167-173
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    • 2002
  • This experiment was conducted to figure out the change of soil physical properties, rice growth and yield with the years of continuous cultivation in direct dry-seeding and no-tillage machine transplanting. Experiments were conducted at NHAES(National Honam Agricultural Experiment Station, RDA, Iksan, Cheon Buk Province, South Korea) with a rice variety "Dongjinbyeo" from 1995 to 2000. In no-tillage machine transplanting cultivation, organic matter in soil was higher than that on direct dry-seeding and was significantly high in topsoil. Problematic weed species were E. crus-galli B., A. keisak H., and L. japonica M. Plant height and tiller number m-2 were higher in common-tillage during the total growth duration. The highest weedy rice occurrence of 27.5% was observed in live years' continuous direct dry-seeding and followed by 6.2%, in four years', and 3.7%, in three years'. The highest yield reduction of 38% was observed in five years' continuous direct dry-seeding. The reduction may resulted from the competition between weedy rice and cultivated rice.