• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.639-643
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• 2002

Water quality, bacterial phase and fish growth rate were analyzed in the process of artificial seed production of flounder (Paralichtys oliraceus) larvae to investigate the water quality in rearing tank using Ultra Filtration System (UES). Sand Filtration System (SFS) and Ultra Filtration System (Ins) were set up in the experimental group. For the analysis of water quality, pH, salinity, DO, SS, COD, $NH_{4}^{+},\;NO_{2}^{-},\;NO^-,\;DIN$ (dissolved inorganic nitrogen) and DU (dissolved inorganic phosphate) were measured. There was no data difference between SFS group and UES group in most analysis items, but the UEs group showed low salinity and low 55 values, such that salinity was $33.5\%_{\circ}$ in SES group and $30.2\%_{\circ}$ in WS group and 55 was 15.5 mL/L in SES group and 7.0 mL/L for UPS group. For changes in bacterial phase and TBC (Total Bacterial Counts), in SES group, 6$\times$10^{5}CFU/mL in seawater decreased to the ratio of about 116, and TBC, Genus Vibrio and bacteria in the Genus Acinetobacter and Genus Micrococcus sharply increased after nine days, while stable bacterial phase was maintained low in UES group during the experiment except for Genus Ajteromonas. In the growth of the larvae, fish length was 17.0 mm (SGR 14.0) in the SES group and 18.8 mm (SGR 14.3) in the UFS group. It is concluded that when water is supplied for artificial seed production with WS, stabilization of water quality condition and inhibition of bacterial multiplication are possible. When production environment becomes stable, stable growth of fish becomes possible by reduction of environmental stress.

• Restorative Dentistry and Endodontics
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• v.35 no.2
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• pp.125-133
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• 2010

Objectives: Rapid polymerization of overlying composite resin causes high polymerization shrinkage stress at the adhesive layer. In order to alleviate the shrinkage stress, increasing the light intensity over the first 5 seconds was suggested as an exponential curing mode by an LED light curing unit (Elipar FreeLight2, 3M ESPE). In this study, the effectiveness of the exponential curing mode on reducing stress was evaluated with measuring microtensile bond strength of three adhesives after the overlying composite resin was polymerized with either continuous or exponential curing mode. Methods: Scotchbond Multipurpose Plus (MP, 3M ESPE), Single Bond 2 (SB, 3M ESPE), and Adper Prompt (AP, 3M ESPE) were applied onto the flat occlusal dentin of extracted human molar. The overlying hybrid composite (Denfil, Vericom, Korea) was cured under one of two exposing modes of the curing unit. At 48h from bonding, microtensile bond strength was measured at a crosshead speed of 1.0 mm/min. The fractured surfaces were observed under FE-SEM. Results: There was no statistically significant difference in the microtensile bond strengths of each adhesive between curing methods (Two-way ANOVA, p > 0.05). The microtensile bond strengths of MP and SB were significantly higher than that of AP (p < 0.05). Mixed failures were observed in most of the fractured surfaces, and differences in the failure mode were not observed among groups. Conclusion: The exponential curing method had no beneficial effect on the microtensile dentin bond strengths of three adhesives compared to continuous curing method.

• Journal of Chest Surgery
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• v.40 no.1 s.270
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• pp.32-36
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• 2007

Background: Although acute renal failure (ARF) after coronary artery bypass graft (CABG) is relatively rare, but devastating complication with high mortality. Our study aims to evaluate the effectiveness of early application of CRRT in patients with ARF which developed after on-pump CABG. Material and Method: Two hundred and eighty seven patients underwent isolated on-pump CABG between May 2002 and Feb. 2006 at our institution, of whom 15 (5.2%) needed CRRT (11 patients for postoperatively developed ARF and the remaining 4 patients with preexisting dialysis-dependent chronic renal failure (CRF) for postoperative hemodynamic and metabolic control). Criteria for early application of CRRT were as follows; decreased urine output less than 0.5cc/h/kg for 2 consecutive hours and elevated serum creatinine level greater than 2.0 mg/dL. Result: The incidence of ARF requiring CRRT after on-pump CABG was 3.9% (11/283) and the overall hospital mortality of patient with CRRT was 33.3% (5/15). Of 5 deaths, 4 were patients with postoperatively developed ARF, and 1 was a patient with pre-existing dialysis-dependent CRF patient. The mean time between the operation and the initiation of CRRT was $25.8{\pm}5.8$ hours and the mean duration of CRRT was $62.1{\pm}41.2$ hours. Of the 7 survivors who were not on dialysis-dependent preoperatively, 6 patients fully recovered renal function during hospital stay and 1 patient required permanent renal supportive treatment after discharge from hospital. Conclusion: Early application of CRRT could maintain stable postoperative hemodynamic status and make outcomes better than those of previous reports in patients with ARF which developed after on-pump CABG.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.153-161
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• 2002

Sox4, a transcription factor, consists of three functional domains: an HMG-box domain as a DNA binding domain, serine rich region as a transactivation domain and glycine rich region (GRR), an unknown functional domain. Although Sox4 is known to be functionally involved in heart, B-cell and reproductive system development, its physiological function remains to be elucidated. We used pGEX expression system to develop a simple and rapid method for purifying Sox4 protein in suitable forms for biochemical studies of their functions. Unexpectedly, we observed that full-length Sox4 appears to be protease-sensitive during expression and purification in E. coli. To map the protease-sensitive site in Sox4, we generated various constructs with each of functional domains of Sox4 and purified as the GST-Sox4 fusion proteins using glutathione beads. We found that the specific cleavage site for the proteolytic enzyme, which exists in E. coli, is localized within the novel GRR of Sox4. Our study suggest that the GRR of Sox4 may a target for the cellular protease action and this cleavage in the GRR may be involved in regulating physiological function of Sox4. Additionally, our study may provide a useful method for investigating the proteolytic cleavage of the target molecule in E. coli.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.4
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• pp.844-852
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• 2006

This study was to evaluate the effect of Yeongyupaedog-san (YGPDS) on mouse Thl and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of YGPDS extract were measured as well as the amount of ${\beta}-hexosaminidase$ in RBL-2H3 cells and the levels of $TNF-{\alpha}$ and 1L-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with YGPDS extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total IgE, OVA-specific IgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4 T cell were stimulated with anti-CD3 and anti-CD28 for 48 hours in various concentrations of YGPDS extract, it increased proliferation of CD4 cells by 11% in $100\;{\mu}g/^{ml}$ concentration but it showed an inhibition by 37% at $200\;{\mu}g/^{ml}$ CD4 T cells under Th1/Th2 polarizing conditions for 3 days with YGPDS resulted in mild decrease of IFN- g in Thl cells and significant decrease of IL-4 in Th2 cells at $500\;{\mu}g/^{ml}\;and\;100\;{\mu}g/^{ml}$ by 18% and 21%, respectively. YGPDS extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}-hexosaminidase$ in RBL-2H3 cells. Treatment of YGPDS extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in TNF-n production. Oral administration of YGPDS extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum IgE and OVA-specific IgE by 25% and 34% , respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 44% and significant decrease of IL-4 and IL-5 by 56%, and 24%, respectively. The results show that YGPDS does not strongly induce mouse T cells to transform into Thl or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.6
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• pp.1467-1476
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• 2006

This study was to evaluate the effect of Bulhwangeumjeonggi-san (BS) on mouse Th1 and Th2 cells' differentiation and ovalbumin (OVA)-induced allergic inflammation. The proliferation of mouse CD4 T cells and the secretion of Th1/Th2 cytokines under the influence of BS extract were measured as well as the amount ${\beta}$-hexosaminidase in RBL-wH3 cells and the levels of TNF-${\alpha}$ and IL-6 secretion in Raw264.7 cells. BALB/c mice were orally administered with BS extract and simultaneously inoculated with OVA to induce allergic reaction and measure the level of total lgE, OVA-specific lgE and the production of IFN- g, IL-4, IL-5 by the spleen cells. When mouse CD4- T cell were stimulated with anti-CD3 and anti-CD28 for 48 hours in various concentrations of BS extract, it increased proliferation of CD4 cells by 14% in 50 ${\mu}g/m{\ell}$ concentration but it showed an inhibition in higher concentrations. CD4 T cells under Th1/Th2 polarizing conditions for 3 days with BS resulted in mild decrease of IFN- g in Th1 cells and mild increase of IL-4 in Th2 cell at 50 ${\mu}g/m{\ell}$ but the level of IL-4 decreased by 18% at 100 ${\mu}g/m{\ell}$. BS extract had a dose-dependent inhibitory effect on antigen-induced release of ${\beta}$-hexosaminidase in RBL-2H3 cells. Treatment of BS extract on LPS stimulated Raw 264.7 cells showed dose-dependent decrease in TNF-${\alpha}$ production. Oral administration of BS extract on OVA-induced allergic mice showed an inhibitory effect on the levels of total serum lgE and OVA-specific lgE by 50% and 55%, respectively. Culture of spleen cells with OVA resulted in significant increase of IFN- g by 25% and significant decrease of IL-4 and IL-5 by 53%, and 38%, respectively. The results show that BS does not strongly induce mouse T cells to transform into Th1 or Th2 but it has an anti-allergic effect in vitro, and that it also corrects the unbalance between the reactions of Th cells in allergic diseases.

• Pediatric Infection and Vaccine
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• v.22 no.3
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• pp.172-177
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• 2015

Purpose: This study aimed to analyze the epidemic period of RSV infection and evaluate the appropriate time of palivizumab immunoprophylaxis. Methods: From January 1991 to July 2012, nasopharyngeal (NP) aspirates were obtained from patients who visited Seoul National University Children's Hospital for respiratory symptoms. NP samples were used to detect respiratory viruses. Among them, we analyzed the positive number and detection rate of RSV infection in two-week interval. The beginning of RSV season was defined when RSV positive number was more than 4 and RSV detection rate was over 10%. From January 2007 to March 2014, we analyzed the starting time of palivizumab immunoprophylaxis for the infants at high risk. Results: The RSV detection rate was 2,013/21,698 (9.69%) over 22 years. The median RSV season was from $2^{nd}-3^{rd}$ week of October to $1^{st}-2^{nd}$ week of February. The earliest starting week was the 3rd week of July in year 2001, and the latest end week was the 3rd week of May in year 1990. Palivizumab immunoprophylaxis was initiated most frequently at the 3rd week of October (18.7%). However, the percentage of starting palivizumab on the 1st week of September has increased from 3.8% in the year 2007 to 14.1% in 2013. Conclusions: The year to year variability of RSV season exists. The starting time of palivizumab immunoprophylaxis should be adjusted based on the season of RSV epidemic.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.56-64
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• 2013

Recent unusual weather due to global warming causes shortage of daily sunlight and constitutes one of the primary reasons for agricultural damages. LED light sources are frequently utilized to compensate for the shortage of sunlight in greenhouse agriculture. The present study is aimed at evaluating formations of phytochemicals as well as growth characteristics of mature strawberry fruits ('Daewang' cultivar) during cultivation in a closed growth chamber equipped with artificial LED light as a sole light source. Each LED light of blue (448 nm), red (634 and 661 nm) or mixed blue plus red (blue:red = 3:7) was separately supplied and the intensity of each light was adjusted to $200{\pm}1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ at plant level with a photoperiod consisted of 16 hours light and 8 hours darkness. Strawberries grown under mixed LED light of blue and red wavelengths showed a higher production of fruits than those grown under other LED treatments. Fructose, one of the free sugars, increased in mixed LED light-grown fruits. Anthocyanin contents were elevated remarkably in the mixed LED light-grown fruits compared with those in other LED treatments. Contrastingly, contents of total phenolics and flavonoids were not of much different from one another among the fruits treated with various LED lights. On the other hand, ripening of strawberry fruits was found to be faster when grown under blue LED light compared with other LED treatments. Moreover, antioxidant activities of blue or red LED light-grown fruits, respectively, were significantly higher than those of mixed LED light-grown fruits. We suggest that when daylight is in shortage during cultivation in a greenhouse, supplementation of sunlight with LED light, which is composed of blue and red wavelengths, could be useful for the enhancement of productivity as well as of free sugar content in strawberry fruits. In addition, for the strawberry culture in the plant factory, selective adoption of LED light wavelength would be required to accomplish the purpose of controlling fruit maturation time as well as of enhancing contents of sugars and antioxidants of fruits.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.50-55
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• 2013

The objective of this research was to investigate the impact of Ca fertilizer on the growth of mother plants, physiological disorders, and occurrence of daughter plants in propagation of 'Seolhyang' strawberry through plastic bag cultivation. To achieve this, chemicals of 1.125 mM $Ca(OH)_2$, 0.375 mM $MgCl_2$, 1.250 mM KCl were blended and designated to combined fertilizer (CF). Then, the effect was compared to the treatment of no Ca application (control) and a commercial fertilizer, CalMag (13-0-1.9-16-6:N-$P_2O_5$-$K_2O$-CaO-MgO), when those were applied every two weeks with controlled electrical conductivity (EC) of solution such as 0.6 or 1.0 $dS{\cdot}m^{-1}$. The number of plants showing Ca deficiency symptoms was the highest in control treatment, but those decreased significantly in CF or CalMag treatments. The differences between CF and CalMag in appearance of Ca deficient plants were not significant when two fertilizers were applied with same EC. The fresh and dry weights of mother plants were the greater in the treatments of CF or CalMag than those of control treatment. The treatments of 1.0 $dS{\cdot}m^{-1}$ of CF or CalMag were more effective than those of 0.6 $dS{\cdot}m^{-1}$ on the fresh and dry weight of the mother plants. The runner lengths in the treatment of 1.0 $dS{\cdot}m^{-1}$ of CF were the longest among the all treatments tested. The treatments of 1.0 $dS{\cdot}m^{-1}$ were more effective than those of 0.6 $dS{\cdot}m^{-1}$ of CF or CalMag in lengths and fresh and dry weight of runners. The fresh and dry weights of daughter plants were heavier in the treatments of CF than those of CalMag. Based on the above results, we concluded that the Ca deficiency in raising 'Seolhyang' strawberry can be lessened by the application of Ca containing fertilizers, but combined application of Ca, K and Mg was more effective than that of Ca alone for seedling production.

• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.181-190
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• 2003

An investigation was conducted on the aquatic environment of the Okchon Stream watershed six times from May to September 2002. The results of investigation revealed that variation of environmental factors were quite significant for each stream and reach, showing a significant difference between running water and stagnant water. Aquatic nutrients were relatively low in the upstream, gradually increasing as the influx of treated wastewater into the stream increased. This suggests that the point source definitely affected the nutrient content of the stream. In particular, the variations of SRP and $NH_4$ were very distinct in the watershed compared to other nutrients. Thus, it can be considered as a major factor in evaluating the effect of treated wastewater. Immediately after the influx of treated waste-water, the average content of SRP rose to 919.3 ${\mu}g$ P/l. This was a very effective level in the watershed, suggesting that the percentage of the nutrients in the water was controlled by the content of P. The constant supply of treated wastewater was found to be a critical factor in triggering the increase in chl-a in the embayment of the stream. With the proliferation of the blue-green algae, the content of chl- a ranged 234.5${\sim}$1,692.2 ${\mu}g/l$. The maximum standing crops exceeded $1.0{\times}10^6$ cells/ml in August, which was more than 200 times the level for red tide in the freshwater. This result was well reflected in other environmental factors, with 100% of AFDM/TSS reflecting the severity of water pollution by algae. Therefore, the reduction of P and N con-tents in the treated wastewater is critical in improving the aquatic environment of the stream as well as water quality management for the reservoir.