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Growth and Microsomal ATPase Activity of Lettuce(Lactuca sativa. L.) Cultured in the $KNO_3-Added$ Nutrient Solution (($KNO_3$를 첨가한 양액에서 상추의 생육 및 마이크로솜 ATPase 활성 변화)

  • Lee, Gyeong-Ja;Kang, Bo-Goo;Kim, Hyun-Ju;Min, Kyeong-Beom;Kim, Young-Kee
    • Korean Journal of Environmental Agriculture
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    • v.20 no.1
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    • pp.28-33
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    • 2001
  • Lettuces were grown hydroponically in three different nutrient solutions, normal and 30 or 50 mM $KNO_3-added$ nutrient solutions, and the electrical conductivities of the nutrient solutions were 1.0, 4.5, and 6.5 dS/m, respectively. Lettuces grown in the $KNO_3-added$ nutrient solutions showed a decrease in the germination ratio and the lower indices of growth, such as plant height, stem diameter, leaf length, and leaf width. Microsomes were prepared from the roots of lettuce and characteristics of microsomal ATPases were investigated. The activities of microsomal ATPases grown in the 30 mM and 50 mM $KNO_3-added$ nutrient solutions were higher than that grown in the normal nutrient solution. The highest activities of microsomal ATPases were observed at pH 7.0 in all culture conditions. The activities of microsomal ATPases were increased in a reaction buffer solution containing high concentration of $K^+$, whereas they were decreased in a reaction buffer containing $Na^+$. The stimulating effect of $K^+$ in the reaction buffer was greater on the microsomal ATPases of lettuces grown in the $KNO_3-added$ nutrient solutions than that grown in the normal nutrient solution. These results imply that the activities of microsomal ATPases in the root tissue are increased as increasing the $KNO_3$ concentration in the hydroponical nutrient solution.

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Dissolution Profile Analysis of Hydroxypropyl Methylcellulose-based Vitamin C Tablets (Hydroxypropyl methylcellulose를 활용한 비타민 C 지속성 정제의 용출 특성 분석)

  • Cha, Ja-Hyun;Hong, Jun-Kee;Lee, Sung-Wan;Cha, Jae-Uk;Ko, Won-Hwa;Baek, Hyon-Ho;Park, Hyun-Jin
    • Korean Journal of Food Science and Technology
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    • v.44 no.3
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    • pp.274-279
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    • 2012
  • The objective of this study was to develop oral matrix tablets for the sustained release of vitamin C. In this study hydroxypropyl methylcellulose (HPMC) has been utilized as an excipient, as it is one of the most widely used polymers, for use during long periods of time in formations. The vitamin C tablet formulation depends on the molecular weight and concentration of sustained-delivery in HPMC. Anti-oxidants have been added as a dissolution medium in order to prevent vitamin C degradation in water. The dissolution test was carried out in a distilled water medium, and the release model equation was applied to analyze the vitamin C release pattern. The results demonstrated that the release and lasting power of vitamin C tablets, containing HPMC, lasted for more than 12 h.

Effect of Long-day and Night-break Treatment on Growth and Anthesis of Orostachys japonicus A. Berger (장일과 암기중단 처리가 바위솔의 생장과 개화에 미치는 영향)

  • 강진호;박진서;김재우
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.5
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    • pp.600-607
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    • 1995
  • Orostachys japonicus, called Wasong and used as anti-tumor medicinal plant, was cultivated in plastic house. The experiment was done to clarify the effect of long-day and night-break treatment at the timing of bolting on its morphological characters, organ dry weight and flowering of florets. After grown in 15cm plastic boxes containing 2:1 soil:peat moss mixture for about 4 months, long-day of 16 hours and night-break of 2 hours around midnight were treated from Sept. 9. The plants were sampled 5 times at 2-week interval after the treatments. Long-day and night-break treatment delayed the growth of inflorescence and showed greater stem diameter on the last sampling and no. of leaves and bracts than the natural daylength. The treatments also had greater leaf and bract dry weight since 2 weeks, and the other fraction and total dry weights since 4 weeks but less floret dry weight from 4 to 6 weeks after the treatments than the natural daylength. The treatments, however, decreased no. of flowered florets and ratio of flowering plants although all the treatments showed nearly the same no. of total florets per plant until 6 weeks after the treatments, late October, which resulted in the modification of source to sink or vice versa. In the natural daylength, the florets were functioned as sink, while root, leaf and bract as source, but in the long-day and night-break treatments stem and florets were done as sink.

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Bioconjugation by dual heterobifunctional coupling method: Use of the conjugates for the detection of dopamine (서로 다른 두 작용기를 이용한 결합법에 의한 접합체: 도파민 면역분석법)

  • Ryu, Ji-Eun;Rhee Paeng, In-Sook
    • Analytical Science and Technology
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    • v.23 no.6
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    • pp.537-543
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    • 2010
  • Dopamine (DA) is an important neurotransmitter molecule of catecholamines. Its deficiency could lead to brain disorder such as Parkinson's disease and schizophrenia. Therefore, it is necessary to establish a suitable analytical technique with sensitivity and simplicity. A competitive enzyme-linked immunosorbent assay for DA has been optimized and characterized. Assay sensitivity is controlled by two factors in competitive immunoassay. One is a nature and concentration of competitor, and the other is those of binder, antibody. Thus, optimization was performed: BSA-DA conjugate and antibody-avidin conjugate were prepared by dual heterobifunctional coupling method using SATA and SMCC. Assay condition was optimized with $6.66\;{\mu}gmL^{-1}$ of BSA-DA and $4.17{\times}10^{-10}\;M$ of antibody-avidin conjugate. A dose-response curve was constructed, and a limit of detection and a dynamic range for DA were accomplished to $2.3{\times}10^{-2}\;{\mu}g\;mL^{-1}$ and four orders of magnitude ($1.0{\times}10^{-7}\;M$ to $1.0{\times}10^{-3}\;M$), respectively. Calibration curve was constructed on dynamic range and least-squares regression of this data gave the following relationship: absorbance = -0.1098 log[DA]+0.0353 ($R^2$ = 0.9956).

Persuasion Effects of Imagery Information Processing caused by Brand in Advertisement Design (광고디자인에 있어서 브랜드명에 의해 유발된 심상정보처리의 설득효과에 관한 연구)

  • Lee Jin-Ryeol;You Si-Cheon
    • Science of Emotion and Sensibility
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    • v.8 no.3
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    • pp.177-187
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    • 2005
  • This article examined how the amount of information presented in ad design affect consumer's evaluation on the ad Existing researches have mainly considered external information presented in the ad from the resource principle perspective. This study investigated how internal information processing such as imagery information processing caused by brand names moderates persuasion effects according to the amount of external information through two experiments. The experiments leave us the conclusion that brand names presented in the ads stimulate imagery information processing when surplus cognitive resources do exist and they lead ad recipients' evaluation to positive direction in prestige brand condition and to the contrary to negative direction in non-prestige brand condition. The results contribute to the direction of ad design in that ad needs not contain as muck product-related information as needed in order to increase persuasion effects in prestige brand condition. Rather, it's necessary to leave the room for internal information processing such as imagery information processing by well structured brand equity. On the contrary, non-prestige brand needs to contain explicit product-relevant information to exert a potent influence on a4 persuasion. We hope this study result is helpful for design direction of advertisement in order to heighten persuasion effects toward ad recipients.

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Preparation of Flexible 3D Porous Polyaniline Film for High-Performance Electrochemical pH Sensor (고성능 전기 화학 pH 센서를 위한 유연한 3차원 다공성 폴리아닐린 필름 제조)

  • Park, Hong Jun;Park, Seung Hwa;Kim, Ho Jun;Lee, Kyoung G.;Choi, Bong Gill
    • Applied Chemistry for Engineering
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    • v.31 no.5
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    • pp.539-544
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    • 2020
  • A three-dimensional (3D) porous polyaniline (PANI) film was fabricated by a combined photo-and soft-lithography technique based on a large-area nanopillar array, followed by a controlled chemical dilute polymerization. The as-obtained 3D PANI film consisted of hierarchically interconnected PANI nanofibers, resulting in a 3D hierarchical nanoweb film with a large surface and open porous structure. Using electrochemical measurements, the resulting 3D PANI film was demonstrated as a flexible pH sensor electrode, exhibiting a high sensitivity of 60.3 mV/pH, which is close to the ideal Nernstian behavior. In addition, the 3D PANI electrode showed a fast response time of 10 s, good repeatability, and good selectivity. When the 3D PANI electrode was measured under a mechanically bent state, the electrode exhibited a high sensitivity of 60.4 mV/pH, demonstrating flexible pH sensor performance.

Alteration of Stress Fiber in Fibroblastic Reticular Cells via Lymphotoxin β Receptor Stimulation is Associated with Myosin (Lymphotoxin β 수용체를 통한 fibroblastic reticular cell의 stress fiber 변화와 myosin의 연관성)

  • Kim, Min Hwan;Kim, Yeon Hee;Choi, Woobong;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.25 no.5
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    • pp.585-593
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    • 2015
  • Stress fiber (SF) alteration is mediated by cellular receptors, which, upon interaction with the extracellular counterpart, signal to the actin cytoskeleton for remodeling. This association is mediated by a variety of scaffold and signaling factors, which control the mechanical and signaling activities of the interaction site. The heterotrimeric transmembrane lymphotoxin α1β2 (LTα1β2), a member of the tumor necrosis factor (TNF) family of cytokines, including soluble homotrimeric lymphotoxin (LT α), plays an important role in lymphoid tissue architecture. Ligation between LTα1β2 and the lymphotoxin β receptor (LTβR) activates signal-cascade in fibroblastic reticular cells (FRCs). We found LTβR stimulation using an agonistic anti-LTβR antibody alone or combined with LTα or TNFα induced changes in the actin and plasticity of cells. To clarify the involvement of myosin underlying the alteration, we analyzed the effect of myosin light chain kinase (MLCK) with an MLCK inhibitor (ML7), the phosphorylation level of myosin light chains (MLC), and the level of phospho-myosin phosphatase target subunit 1 (MYPT1) after treatment with an agonistic anti-LTβR antibody for cytoskeleton reorganization in FRCs. The inhibition of MLCK activity induced changes in the actin cytoskeleton organization and cell morphology in FRC. In addition, we showed the phosphorylation of MLC and MYPT1 was reduced by LTβR stimulation in cells. A DNA chip revealed the LTβR stimulation of FRC down-regulated transcripts of myosin and actin components. Collectively, these results suggest LTβR stimulation is linked to myosin regarding SF alteration in FRC.

Hepatic Vessel Segmentation using Edge Detection (Edge Detection을 이용한 간 혈관 추출)

  • Seo, Jeong-Joo;Park, Jong-Won
    • Journal of the Korea Society of Computer and Information
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    • v.17 no.3
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    • pp.51-57
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    • 2012
  • Hepatic vessel tree is the key structure for hepatic disease diagnosis and liver surgery planning. Especially, it is used to evaluate the donors' and recipients' liver for the LDLT(Living Donors Liver Transplantation) and estimate the volumes of left and right hepatic lobes for securing their life in the LDLT. In this study, we propose a method to apply canny edge detection that is not affected by noise to the liver images for automatic segmentation of hepatic vessels tree in contrast abdominal MDCT image. Using histograms and average pixel values of the various liver CT images, optimized parameters of the Canny algorithm are determined. It is more time-efficient to use the common parameters than to change parameters manually according to CT images. Candidates of hepatic vessels are extracted by threshold filtering around the detected the vessel edge. Finally, using a system which detects the true-negatives and the false-positives in horizontal and vertical direction, the true-negatives are added in candidate of hepatic vessels and the false-positives are removed. As a result of the process, the various hepatic vessel trees of patients are accurately reconstructed in 3D.

Synthesis and Latent Characteristics of Thermal Cationic Latent Catalysts by Change of Substituent (치환기 변화에 따른 열잠재성 양이온 촉매의 합성과 잠재특성 연구)

  • Park, Soo-Jin;Heo, Gun-Young;Lee, Jae-Rock;Shim, Sang-Yeon;Suh, Dong-Hack
    • Polymer(Korea)
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    • v.25 no.4
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    • pp.558-567
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    • 2001
  • The syntheses of thermal latent catalysts have been carried out by modifying the substituent of pyrazinium salts. The thermal latent properties and cure behaviors of difunctional epoxy resin (diglycidylether of bisphenol-A, DGEBA) with 1 wt% of catalyst as an initiator were investigated by dynamic DSC method. As a result, the synthesized catalysts showed the good latent thermal properties in epoxy system. With increasing the basicity of substituted catalyst, the cure temperature and activation energy of epoxy system were increased, whereas the activity was decreased. This was probably due to the fact that the activity and cure behavior were controlled by ring strain and basicity of substituent. Consequently, the catalyst activity modified by methyl group as an electron donor was decreased in increasing of basicity in an initiation step of epoxy cure system. This is due to a decreasing of stabilities of both leaving group of pyrazinium salts and benzyl cation. However, the catalyst activity modified by cyano group as an electron acceptor was increased in increasing the stability of benzyl cation resulting from organic effects and resonance.

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A Study on the Warpage of Glass Fiber Reinforced Plastics for Part Design and Operation Condition: Part 1. Amorphous Plastics (유리섬유로 보강된 수지에서 제품설계 및 성형조건에 따른 휨의 연구: Part 1. 비결정성 수지)

  • Lee, Min;Kim, Hyeok;Lyu, Min-Young
    • Polymer(Korea)
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    • v.36 no.5
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    • pp.555-563
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    • 2012
  • Warpage of injection molded product is caused by non-uniform shrinkage during shaping operation and relaxation of residual stress. Robust part design and glass fiber reinforced reins have been adopted to prevent warpage of part. Warpages for part designs have been investigated in this study according to the injection molding conditions. Part design contains flat specimen and two different rib designs in the flat part. Resins used in this study were glass fiber reinforced amorphous plastics, PC and ABS. Different rib designs showed significant differences of warpages in the parts. Various warpages have been observed in the three regions of the part, near gate region, opposite region to the gate, and flow direction region. Results of computer simulation revealed that the warpages were strongly related to glass fiber orientation. Flat specimen showed the smallest warpage and the specimen with ribs to the flow direction showed a high resistance to warpage. Warpage highly depended upon part design rather than molding condition. It was concluded that the rib design and selection of gate location in injection molding would be the most important factors for the control of warpage since those are directly related to the fiber orientation during molding.