• Title/Summary/Keyword: 조작주의

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Establishment and Characterization of Clonal Cell Lines from Zebrafish, Danio rerio (제브라피쉬(Danio rerio) 배아로부터 동형세포주 확립)

  • Lee, Ki-Young
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.1-6
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    • 2008
  • Three types of clonal cell lines were isolated according to their size and phenotype from the adherent cell populations in long-term liquid cultures from the embryonic fibroblast cells of Zebrafish, Danio rerio. All kind of cell lines were well proliferated. The size and number of clonal cell lines derived colonies from stable embryonic cells were significantly increased in the presence of NAC and A2P conditioned medium from the cell lines. The stable cell lines and clonal cell lines were cap-able of well proliferation in vitro. These cell lines have been maintained in continuous culture without change in characteristics. A majority of the clonal cells (80%) was shown a normal chromosomal complement (50 chromosomes, 2N) in according with FACs analysis. Majority of cells were positive to vimentin staining and none of them were positive for nestin and Oct -4 by immunocytochemistry. These results indicate that the clonal cell lines obtained from cultured cells are fibroblasts and may be extremely useful in genetic manipulation for further nuclear transfer and fish cloning.

A Study on Measures to Reduce Traffic Accidents caused by Using Smartphones While Driving (운전 중 스마트폰 사용으로 인한 교통사고 저감대책 연구)

  • You, Seung-Hee
    • Journal of Digital Convergence
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    • v.14 no.7
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    • pp.175-184
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    • 2016
  • The purpose of the study focuses on increasing dangers of using smartphones while driving recently, and then is to come up with measures to reduce traffic accidents caused by using devices like a smartphone. This study conducted a survey of drivers using their smartphones while driving to understand risks caused by using a smartphones while operating vehicles. Results showed that a lot of activities may lead to distracted driving, such as texting, making phone calls, using GPS or road maps, game, etc. In this paper, we presented that functions of smartphone should be controlled partially while driving for safe driving performance. These results suggest that using IoT-based smart devices like a beacon and a smartphone application implemented, tentatively called "Safe driving solution", while driving can reduce traffic accidents. Thus, in order to effectively prevent dangerous driving due to the use of smartphones, a "Safe driving solution" which restricts all functions except for calls and driver assistance functions is suggested.

A Study of Aggressive Driver Detection Combining Machine Learning Model and Questionnaire Approaches (기계학습 모델과 설문결과를 융합한 공격적 성향 운전자 탐색 연구)

  • Park, Kwi Woo;Park, Chansik
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.7 no.3
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    • pp.361-370
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    • 2017
  • In this paper, correlation analysis was performed between questionnaire and machine learning based aggressive tendency measurements. this study is part of a aggressive driver detection using machine learning and questionnaire. To collect two types tendency from questionnaire and measurements system, we constructed experiments environments and acquired the data from 30 drivers. In experiment, the machine learning based aggressive tendency measurements system was designed using a driver behavior detection model. And the model was constructed using accelerate and brake position data and hidden markov model method through supervised learning. We performed a correlation analysis between two types tendency using Pearson method. The result was represented to high correlation. The results will be utilize for fusing questionnaire and machine learning. Furthermore, It is verified that the machine learning based aggressive tendency is unique to each driver. The aggressive tendency of driver will be utilized as measurements for advanced driver assistance system such as attention assist, driver identification and anti-theft system.

Development of the motivating efficacy scale for mathematics teachers (수학교사의 수학 학습동기 유발 효능감 측정 도구 개발 연구)

  • Somin Kim;Hee-jeong Kim
    • Journal of the Korean School Mathematics Society
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    • v.26 no.2
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    • pp.159-184
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    • 2023
  • In this study, after defining motivating efficacy operationally, we developed a draft of the Motivating Efficacy Scale for Mathematics Teachers (MESMT), a measure of mathematics teachers' motivating efficacy, through the literature review and an expert Delphi survey, and conducted the exploratory factor analysis using online survey responses from 347 elementary and secondary mathematics teachers across the country to explore the factor structure of the measure and to test its validity and reliability. The exploratory factor analysis resulted in the deletion of 17 items from the initial 42 items developed through the literature review and expert Delphi survey and the identification of four factors (Providing successful experiences, Eliciting attention and engagement, Creating mathematics case-based relevance, and Providing extrinsic rewards), resulting in a final MESMT of 25 items. The MESMT developed in this study is a valid and reliable measure of mathematics teachers' motivating efficacy, and is expected to serve as a starting point for many subsequent studies to understand mathematics teachers' motivating efficacy and improve mathematics teachers' ability to motivate students' mathematics learning.

Strategies for Increasing the Value and Sustainability of Archaeological Education in the Post-COVID-19 Era (포스트 코로나 시대 고고유산 교육의 가치와 지속가능성을 위한 전략)

  • KIM, Eunkyung
    • Korean Journal of Heritage: History & Science
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    • v.55 no.2
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    • pp.82-100
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    • 2022
  • With the crisis of the COVID-19 pandemic and the era of the 4th industrial revolution, archaeological heritage education has entered a new phase. This article responds to the trends in the post-COVID-19 era, seeking ways to develop archaeological heritage education and sustainable strategies necessary in the era of the 4th industrial revolution. The program of archaeological heritage education required in the era of the 4th industrial revolution must cultivate creative talent, solve problems, and improve self-efficacy. It should also draw attention to archaeological heritage maker education. Such maker education should be delivered based on constructivism and be designed by setting specific learning goals in consideration of various age-specific characteristics. Moreover, various ICT-based contents applying VR, AR, cloud, and drone imaging technologies should be developed and expanded, and, above all, ontact digital education(real-time virtual learning) should seek ways to revitalize communities capable of interactive communication in non-face-to-face situations. The development of such ancient heritage content needs to add AI functions that consider learners' interests, learning abilities, and learning purposes while producing various convergent contents from the standpoint of "cultural collage." Online archaeological heritage content education should be delivered following prior learning or with supplementary learning in consideration of motivation or field learning to access the real thing in the future. Ultimately, archaeological ontact education will be delivered using cutting-edge technologies that reflect the current trends. In conjunction with this, continuous efforts are needed for constructive learning that enables discovery and question-exploration.

The Dental Hygienists' Perception of the National Practical Examination (치과위생사의 국가 실기시험에 대한 인식)

  • Ko, Da-Kyung;Bae, Sung-Suk
    • Journal of dental hygiene science
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    • v.16 no.6
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    • pp.488-494
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    • 2016
  • The purpose of the present study was to examine dental hygienists' perception of the current national practical examination. This research was performed using 199 self-reported surveys answered by professors of dental hygiene studies and clinical dental hygienist. Frequency analysis, chi-square tests, and analysis of variance were performed by using IBM SPSS Statistics ver. 20.0. The results revealed that many of the respondents consider the current national practical examination to be neutral. They did not think that the current national practical examination questions are useful for assessing occupation-centric integrated clinical practice ability and counseling techniques for patient intervention. The professors of dental hygiene studies believed that among the research tasks required as mentioned in the national practical examination questions, dental polishing and tooth brushing education are of paramount importance, whereas clinical dental hygienists believed that ultrasonic scaling is the most important (p<0.05). Most of the professors of dental hygiene studies reported that they conducted skills education for dental polishing and tooth brushing education, while most of the clinical dental hygienists reported that tasks actually performed in the clinic included impression taking, model fabrication, ultrasonic scaling, and explaining treatment precautions (p<0.05). Therefore, these tasks can be effectively carried out with the improvement of the national dental hygienist practical examination.

Development Discourse and Its Representation in Middle School Geography Textbooks (중학교 지리 교과서에 재현된 개발 담론 분석)

  • Cho, Chul-Ki
    • Journal of the Korean association of regional geographers
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    • v.20 no.4
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    • pp.454-472
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    • 2014
  • This study is to analyze the approaches of development discourse and its representation in geography curriculum and textbooks for middle school in Korea. The paper examines the spectrum of development discourse in terms of modernization theory, dependency theory, neoliberalism, grassroots development, sustainable development, postcolonism, post-structuralism and post-development. The findings on geography textbook analysis based on them are as follows. First, Most of the textbooks don't include the definition and operational definition on development and sustainable development. Second, development indicators rely on normal economic indicators like GDP or GNI per capita. HDI that includes GDP per capita, level of education and life expectancy is treated in some of the textbooks, and gender index is never presented. Third, a textbook still uses biased terms such as developed and most developed countries instead of developing countries. Fourth, in plans to solve economic unequality and geographical problems, personal level is treated less than the global, nation and NGOs. Finally, statements on sustainable development contain only intergenerational equity, not intergenerational equity. And approaches on sustainable development are based on technocentric approaches more than ecocentric approaches. Thus geography textbooks should be carefully written on development discourse by authors.

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Tn5 insertions in the agrocin 84 plasmid the conjugal nature of pAgK84 and the locations of determinants for transfer and agrocin 84 production

  • 심재섭
    • The Microorganisms and Industry
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    • v.12 no.2
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    • pp.2-13
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    • 1986
  • Agrobacterium tumefaciens의 연구는 유전공학 시대를 맞이하여 많은 연구자들의 커다란 주목을 끌고있다. 식물세포내에 외부 유전자를 도입시키는, 확실히 믿을 수 있는 vector로 등장된 때문이다. 원래 이세균은 식물 줄기나 뿌리에 암종을 유발시키므로서 암발성 원인 구명 연구로 흥미를 끌게 되었다. 연구결과는 암발생 예방및 치료에 목적을 둠은 덩연할 것이다. 많은 약제가 시험되었으나 별로 진전을 보지 못하던중 비 원인성인 Agrobacterium radiobacter, strain 84에 의한 생물학적 방제의 성공으로 유일한 방제법을 갖게되었다. 뒤이어 암종발생 기작도 밝혀졌다. Agrobacterium의 세계는 온통 유전공학 기술로 채워져 있다. 암종발생에서 방제원리에 이르기까지 수없이 먼 옛날부터 이미 익혀오던 DNA 조작기술이었던가\ulcorner 암종을 유발시키는 agrocin84 plasmid를 갖는 비병원성 Agrobacterium을 찾아 생물학적 방제법을 확립하였다. 그후 병원성 Agrobacterium은 이에 대하여 어떻게 살아남을 것인가\ulcorner 실로 놀라운 일이라 아니할 수 있을까\ulcorner 이 병원성 Agrobacterium은 비 병원성 Agrobacterium 속에 있는 agrocin 84 plasmid을 탈취하여 자신이 agrocin84를 생성분비하며 암종 유발을 계소하여 간다. 아니면 비병원성 Agrobacterium이 병원성 Agrobacterium에게 agrocin 84 plasmid를 넘겨주었을까\ulcorner 왜 넘겨주었을까\ulcorner 공존을 위하여서일까\ulcorner 우리의 유전공학 기술은 이것을 막아줄수 있을까\ulcorner 생물학적 방제의 재성공을 위하여 논제의 연구는 왜 필요했던가\ulcorner 그 전후를 여기에 서술해 본다.닭이며 또한 제한된 지면에서 충분히 고찰하기는 어렵다. 우리나라에서 자주 거론되는 백신 및 종류에 국한하여 그 문제점과 앞으로의 전망을 고찰해 보기로 한다.ocking electrode를 제작하여 복합고분자 전해질과의 계면저항을 측정하였다.nm (1.2921eV)는 acceptor-bound exciton 인 I1(AO,X) 이고, 964.6nm(1.2853eV)는 donor-acceptor pair(DAP) 발광, 1341.9nm (0.9239eV)는 self activated(SA)에 기인하는 광발광 봉우리로 고찰되었다.가 높을수록 방출전류가 시간에 따라 급격히 감소하였다. 각 duty비에서 방출전류의 양이 1/2로 감소하는 시점을 에미터의 수명으로 볼 때 duty비 대 에미터 수명관계를 구해 높은 duty비에서 전계방출을 시킴으로써 실제의 구동조건인 낮은 duty비에서의 수명을 단시간에 예측할 수 있었다. 단속적으로 일어난 것으로 생각된다.리 폐 관류는 정맥주입 방법에 비해 고농도의 cisplatin 투여로 인한 다른 장기에서의 농도 증가 없이 폐 조직에 약 50배 정도의 고농도 cisplatin을 투여할 수 있었으며, 또한 분리 폐 관류 시 cisplatin에 의한 직접적 폐 독성은 발견되지 않았다이 낮았으나 통계학적 의의는 없었다[10.0%(4/40) : 8.2%(20/244), p>0.05]. 결론: 비디오흉강경술에서 재발을 낮추기 위해 수술시 폐야 전체를 관찰하여 존재하는 폐기포를 놓치지 않는 것이 중요하며, 폐기포를 확인하지 못한 경우와 이차성 자연기흉에 대해서는 흉막유착술에 더 세심한 주의가 필요하다는 것을 확인하였다. 비디오흉강경수술은 통증이 적고, 입원기간이 짧고, 사회로의 복귀가 빠르며, 고위험군에 적용할 수 있고, 무엇보다도 미용상의 이점이 크다는 면에서 자연기흉에 대해 유용한 치료방법임에는

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Development of Reading Comprehensive Strategy Program for Underachieving Gifted Students (중학교 영재학생의 미성취 문제 해결을 위한 중재방안으로서의 읽기 이해 전략 프로그램 개발)

  • Choi, Sun Ill;Jin, Sukun
    • Journal of Gifted/Talented Education
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    • v.25 no.4
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    • pp.649-667
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    • 2015
  • This study handled underachievement issue of gifted students by developing and validating educational programs as a solution, or relief, of their academic underachievement problems. The proposed educational program was designed to enhancing reading comprehension ability of gifted students with underachievement. Based on comprehensive literature review, this study found that key issues of underachievement have to do with learning strategy, and also that learning strategy was established as 'reading comprehension strategy.' Considering diverse reading comprehension models and strategies suggested by preceding studies, the reading comprehension strategy program in Korean was customized for underachieving gifted students on the middle school level. The effectiveness of the reading comprehension strategy program as a solution to underachievement of gifted students was explored by applying the developed program to 36 identified students after school twice a week for 6 weeks, conducting pre- and post-tests that were selected to measure their reading comprehension abilities, collecting their academic achievement data before and after the intervention by this study, and interviewing students. As the results of this study, reading metacognition ability, reading comprehension skills, and school grades of gifted students with underachievement were meaningfully improved as a group. On the individual level, 12 identified students, exactly one third of the whole group of underachieving gifted students, showed so improved academic achievement as we can say they overcame underachievement based on the Supplee's definition.

Monitoring system for packet analysis on Wi-Fi environment (Wi-Fi 환경에서 패킷 분석을 위한 모니터링 시스템)

  • Seo, Hee-Suk;Kim, Hee-Wan;Ahn, Woo-Young
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.12
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    • pp.227-234
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    • 2011
  • Many technologies for wireless internet are increasing as more and more laptop computers, net books, smart phone and other terminals, which provide wireless network, are created. IEEE 802.11 is computer wireless network technology that used in small area, called wireless LAN or Wi-Fi. IEEE 802.11 is a set of standards for implementing wireless local area network (WLAN) computer communication in the 2.4, 3.6 and 5 GHz frequency bands. They are created and maintained by the IEEE LAN/MAN Standards Committee (IEEE 802). AP (Access Point) is installed at cafes and public places providing wireless environment. It is more convenient to use wireless internet, however, It can be seen easily around us and possible to communicate with AP. IEEE 802.11 has many vulnerability, such as packet manipulation and information disclosure, so we should pay more attention when using IEEE 802.11. Therefore this paper developing monitoring system which can find out AP and Stations that connect with it, and capturing AP's information to find out vulnerability. This paper suggests monitoring system which traffic analysis in wireless environment.