• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.359-369
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• 2018

Harmful algal blooms (HABs) are a serious problem for public health and fisheries industries, thus there exists a need to investigate the possible ways for effective control of HABs. In the present study, we investigated the algicidal effects of a newly developed GreenTD against the HABs (Chattonella marina, Heterosigma akashiwo, Cochlodinium polykriokides, and Heterocapsa circularisquama) and non-HABs (Chaetoceros simplex, Skeletonema sp. and Tetraselmis sp.), which is focused on the different population density and concentration gradients of algicidal substances. The time series viability of target alga was assessed based on the activity of Chl. a photosynthetic efficiency in terms of $F_v/F_m$, and in vivo fluorescence (FSU). Effective control of Raphidophyta, C. marina and H. akashiwo was achieved at a GreenTD concentration of $0.5{\mu}gL^{-1}$ and $0.2{\mu}gL^{-1}$, respectively, and regrowth of both the species was not observed even after 14 days. The inhibitory ratio of the dinoflagellate, C. polykriokides was more than 80% at $0.2{\mu}gL^{-1}$ of GreenTD. H. circularisquama was constantly affected in the presence of $0.2{\mu}gL^{-1}$ of GreenTD in the high- and low-population density experimental groups. On the other hand, diatoms, C. simplex, and Skeletonema sp. were not significantly affected even in the presence of $0.2{\mu}gL^{-1}$ of GreenTD and exhibited re-growth activity with the passage of incubation time. In particular, green alga Tetraselmis sp. remained unaffected even in the presence of the highest concentration of GreenTD ($1.0{\mu}gL^{-1}$), implying that non-HABs were not greatly influenced by the algicidal substances. As a result, the algicidal activity of GreenTD on the harmful and nonharmful algae was as follows: raphidophyte>dinoflagellates>diatoms>green alga. Consequently, our results indicate that inoculation of GreenTD substances into natural blooms at a threshold concentration ($0.2{\mu}gL^{-1}$) can maximize the algicidal activity against HABs species. If we consider the dilution and diffusion rate in the field application, it is hypothesized that GreenTD will demonstrate economic efficiency, thus leading to effective control against the target HABs in the closed bay.

• Korean Journal of Ecology and Environment
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• v.40 no.1
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• pp.121-129
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• 2007

This study was conducted to evaluate the causes and effects of cyanobacterial development and succession in a shallow eutrophic reservoir from March 2003 to February 2004. Phytoplankton succession, sedimentation rate, and sediment composition were analyzed. Algal bioassay also was conducted with the consideration of light, water temperature and nutrients. Cyanobacteria dominated throughout the year, except for spring season (March${\sim}$April) in which diatoms and flagellates dominated. Total cell density increased in July and November when P loading through inflows was high. Oscillatoria spp. and Aphanizomenon sp. were dominant in May and June, respectively, but replaced with Microcystis spp. in July. Thereafter, Microcystis spp. sustained until December, and again shifted to Oscillatoria spp. and Aphanizomenon sp. The dominance of Oscillatoria spp. in May was accompanied with high TN/TP ratio and the increase of water temperature and light intensity. While the dominance of Microcystis spp. was related with relatively low TN/TP ratio, ranging from 46 to 13 (average: 27). The sedimentation rate was highest in March (0.6 m $day^{-1}$) when diatoms dominated. During the period of cyanobacterial dominance, relatively high sedimentation rate was observed in May (0.4 m $day^{-1}$) and October (0.36m $day^{-1}$). C/N ratio of the sediment ranged $6{\sim}8$. Inorganic P concentration in the pore water was low when DO concentration was < 2 mg $O_2$ $L^{-1}$ in the hypolimnion, reflecting the P release from the sediment. Cyanobacterial growth rate depended on phosphorus concentration and water temperature, and high P concentration compensated for the low temperature in the growth rate. Our results suggest that the potential of cyanobacterial development and substantiality in eutrophic reservoirs be high throughout the year, as being supplied with enough P, and emphasize the consideration of sediment man. agement for the water quality improvement and algal bloom control.

• Ecology and Resilient Infrastructure
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• v.3 no.1
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• pp.22-34
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• 2016

We developed a biologically-derived substance naphthoquinone (NQ) derivate for the eco-safe mitigation of harmful cyanobacteria blooms such as Microcystis and Dolichospermum. NQ was reacted with various substituents ($R_n$) to produce different NQ derivatives. We tested a total of 92 algicidal compounds based on the algicidal activity of Microcystis and Dolichospermum. 22 compounds of NQ were selected as candidates (algicidal activity >80% at $1{\mu}M$). Among them, NQ 40 compound showed the highest algicidal activity of 99.6% and 100% at the optimal concentration of $1{\mu}M$ on Microcystis and Dolichospermum, respectively. No algicidal effects of NQ 40 ($1{\mu}M$) were observed against non-target algae such as Stephanodiscus, Cyclotella and Peridinium. According to the results of acute eco-toxicity assessment, the $EC_{50}$ values of NQ 40 compound for Selenastrum capricornutum and Daphnia magna were 3.2 and $14.5{\mu}M$, respectively, and the $LC_{50}$ for Danio rerio was $15.7{\mu}M$. In addition, for D. magna chronic eco-toxicity assessment, no toxicity toward survival, growth and reproduction was observed. Therefore, we suggested the NQ 40 ($1{\mu}M$) compound as an alternative eco-safe algicidal substance to effectively mitigate harmful cyanobacteria blooms.

• Analytical Science and Technology
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• v.28 no.3
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• pp.168-174
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• 2015

Cyanobacterial toxins, such as microcystins, which exist in Korean lakes, are strongly toxic in fish, cattle, and humans. This study performs a quantitative analysis of cyanobacterial toxins in water by comparing the strip method and the HPLC method. Because the detection ranges of the strip method and the HPLC method are different, the water samples were diluted. The comparison of the strip method and the HPLC method was made using seven samples that contained different concentrations of microcystin. The quantitative results produced by the strip analysis were significantly aligned with the results of the HPLC analysis. The results of correlation analysis were r = 0.99998 and p = 0.00001.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.362-367
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• 2009

Among total 176 strains with antialgal effects isolated from So-ok stream in Korea, Pseudomonas aeruginosa AJ1 showed the highest removal efficiency for an algal species Microcystis aeruginosa (clear zone of diameter 50.0 mm on algal lawn after 20 days). The algal growth was suppressed even when the supernatant of AJ1 culture was applied, suggesting that extracellular substances are responsible for its antialgal activity. The removal activity of AJ1 was optimal under the following condition: pH 8, $30^{\circ}C$, and mannitol as a carbon source. The antialgal activity of AJ1 appeared to be dependent of the growth phase of M. aeruginosa, i.e., the highest at the early phase, but not its own phase. As expected, the algicidal effect was improved as the amount of the treated supernatant was increased; the highest removal efficiency (80.3%) was achieved when 40 ml/L of the supernatant was used. Interestingly, however, the removal rate was opposite. The highest removal rate ($8.2{\mu}g$ chl-a/ml supernatant/day) was achieved when low concentration (10 ml/L) was applied. These results suggest that P. aeruginosa AJ1 is a promising biological agent to control the problematic algal bloom.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.310-314
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• 2016

The search for natural antioxidants as alternatives to synthetic products is growing. Microalgae have emerged as a source of natural antioxidants with significant and diverse health-promoting properties. In this study, the effects of hot-water extraction conditions on total polyphenol compounds (TPC) production were investigated for lipid extracted microalgae (LEA). In order to enhance the polyphenol productivity, the extraction variables including solvents, temperature, time and ethanol concentration were optimized. The results showed hot-water extraction provided a higher extraction efficiency than the organic solvents and extraction at high temperatures showed a better extraction efficiency. While hot-water extract showed a higher extraction efficiency compared to 98% ethanol extraction, the mixture of water and ethanol (40:60 v/v) showed the highest production of polyphenols. The maximum polyphenols of 3.35 mg GAE (gallic acid equivalent)/g DM were obtained at the optimized extraction time of 10 min, $100^{\circ}C$ and 40% ethanol, respectively. Although Tetraselmis KCTC 12236BP was preprocessed by hexane to remove lipid for bio-diesel production, the results showed LEA contains relatively high level of polyphenols compared to untreated microalgae which can be used in the production of value-added materials. The predictions obtained from the developed Peleg's model were compared with the experimental data under the same operating conditions. The predicted and experimental data were consistent, indicating the reliability of the model.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.52-52
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• 2022

남조류 (cyanobacteria)의 대량증식에 의한 녹조현상은 수질 악화 및 수중 생태계에 악영향을 끼친다. 기존 연구에서는 시아노박테리아 증식에 영향을 미칠 수 있는 다양한 요인을 식별하였다. 특히, 최근 (2020)에 수행된 한 연구는 2013년부터 2018년까지 4대 강에 위치한 16개의 지점에서수집된 세 가지 환경 변수, 수온, 유속 및 인(phosphorus)농도 데이터를 사용하여 시아노박테리아 발생에 대한 예측 모델을 개발하였으며 온도가 남조류에 발생에 대한 가장 지배적인 요인임을 시사하였다. 온실가스 배출에 의한 기온의 상승으로 특징되는 기후변화는 전 지구에 급격한 환경변화를 유발하고 있으며 이는 남조류 증식에도 영향을 줄 수 있다. 이에 본 연구에서는 한국의 4대 강에서 다년간 수집된 수온(℃) 및 녹조 (cells/㎖) 데이터에 기반하여 온도-환경변수 민감도 (scaling)를 분석함으로써 기후변화와 남조류 증식의 연관성을 판단해 보고자 한다.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.517-517
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• 2023

현재 우리나라에서는 4대강 및 주요 호소 29지점을 대상으로 조류경보제가 시행되고 있으며 조류 경보 단계는 실시간 모니터링지점에서 측정되는 유해 조류의 셀농도를 기반으로 발령 단계가 결정된다. 상수원 구간은 관심, 경계, 조류 대발생, 해제 또는 미발생 총 4구간으로 구성되며, 친수 활동 구간의 경우 조류 대발생을 제외한 3구간으로 구성된다. 현재 시행되는 조류 경보제의 목적은 유해 조류 발생 시 사후 대응 방안 마련에 보다 초점이 맞춰져 있으며 특히, 모니터링 주기 확대 여부, 오염원 관리 방안 마련, 조류 제거 여부 등의 의사 결정 수단으로 사용되고 있다. 하지만 조류 경보 단계에 대한 사전 예측이 가능한 경우 유해 조류의 성장을 억제할 수 있으며 이를 통해 안전하고 깨끗한 수자원을 확보할 수 있다. 본 연구에서는 조류 경보 단계의 사전적 예측을 위해 국가 실시간 측정망에서 제공하는 전국 보 모니터링 종합 정보 자료, 기상측정망 자료, 실시간 보 현황 자료를 활용하여 예측 모델을 구축하였다. 또한, 단계 예측의 정확도를 개선하기 위해 변수 선택 기법을 활용하여 조류 경보 단계에 영향을 미치는 환경변수를 선정하였으며 자료의 불균형으로 인해 모델 학습 과정에서 발생하는 예측 오류를 최소화하기 위해 다양한 샘플링 기법을 적용하여 모델의 성능을 평가하였다. 변수 선택 및 샘플링 기법을 고려하지 않은 원자료를 사용하여 예측 모델을 구축한 결과 관심 단계(Level-1) 및 경보 단계(Level-2)에 대해 각각 50%, 62.5%의 예측 정확도를 보인 반면 비선형 변수 선택 기법 및 Synthetic Minority Over-sampling Technique-Edited Nearrest Neighbor(SMOTE-ENN) 샘플링 기법을 적용하여 구축한 모델에서는 Level-1은 85.7%, Level-2는 75.0%의 예측 정확도를 보였다.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.508-514
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• 2017

Water-bloom and red tide due to eutrophication have been overgrown and have caused various environmental problems. Recently, however, research on bid-diesel that can utilize algae as an energy source has been actively carried out. In particular, many studies variously have been conducted to utilize algal photosynthesis oxygen as a supply method for reducing the energy by an air blower in MWTP. In this study, a lab scale algae-nitrification reactor was operated to replace the oxygen required for nitrogen removal and the operation period was largely divided into three sections. In the first section, ammonia nitrogen removal efficiency was 24 ~ 38% according to the MLSS (Mixed Liquer Suspended Solid) concentration. In the second section, ammonia nitrogen removal efficiency was 38 ~ 50% according to the micro-algae concentration and in the last section ammonia nitrogen removal efficiency was 61 ~ 80% according to HRT (Hydraulic Retention Time). As a result, as the MLSS decreased and algae biomass increased, the ammonia nitrogen removal efficiency tended to increase, but the effect of Algae biomass was greater than that of MLSS.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.186-191
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• 1999

The three speices of miroalgae (Chlorella vulgaris, Dunaliella salina and Porphyridium purpureum) were immobilized in Ca-alginate capsules as a basic study for development of economic cultivation process, and then were cultivated in an air-bubble column bioreactor. Under the batch culture of aerobic conditions, the thickness of the capsule membrane and $CO_2$ supply did not affect the growth of the immobilized microalga, Chlorella vulgaris. Cell concentration of immobilized microalgae in the capsule was higher than those of imobilized microalgae in beads and free cells. The cell concentration of microencapsulated Dunaliella salina was greater about 5 times than that of free cells. Based on these results, it is concluded that the application of microencapsulation technology to the culture of microalgae was an effective method for high-density cultivation.