• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.801-804
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• 1994

The early change observed in lead-induced neurophathy in the rat was Schwann cell swelling. In order to quantify this cell swelling, Schwann cell thickness and major diameter of the nucleus were measured using tranverse section with associated myelinated fiber of sciatic nerves. Group I rats were intoxicated with 0.5% lead acetate in the drinking water for 30 days; group II animals were treated as in group I and then restored to normal laboratory conditions for 30 days; and group III were controls. The results showed that the cell sizes were significantly greater in intoxicated animals, compared with control, and the cell sizes of group II did not differ significantly from control rats.

• Journal of Chest Surgery
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• v.37 no.8
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• pp.632-643
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• 2004

Background: The Histidine-Tryptophan-Ketoglutarate (HTK) solution has been shown to provide the excellent myocardial protection as a cardioplegia. The HTK solution has relatively low potassium as an arresting agent of myocardium, and low sodium content, and high. concentration of histidine biological buffer which confer a buffering capacity superior to that of blood.. Since HTK solution has an excellent myocardial protective ability, it is reported to protect myocardium from ischemia for a considerable time (120 minutes) with the single infusion of HTK solution as a cardioplegia. The purpose of this study is to evaluate the cardioprotective effect of HTK solution on myocardium when the ischemia is. exceeding 120 minutes at two different temperature (10 to 12$^{\circ}C$, 22 to 24$^{\circ}C$) using the Langendorff apparatus, Material and Method: Hearts from Sprague-Dawley rat, weighing 300 to 340 g, were perfused with Krebs-Henseleit solution at a perfusion pressure of 100 cm $H_2O$. After the stabilization, the heart rate, left ventricular developed pressure (LVDP), and coronary flow were measured. Single dose of HTK solution was infused into the ascending aorta of isolated rat heart and hearts were preserved at four different conditions. In group 1 (n=10), hearts were preserved at deep hypothermia (10∼12$^{\circ}C$) for 2 hours, in group 2 (n=10), hearts were preserved at moderate hypothermia (22∼24$^{\circ}C$) for 2 hours, in group 3 (n=10), hearts were preserved at deep hypothermia for 3 hours, and in group 4 (n=10), hearts were preserved at moderate hypothermia for 3 hours. After the completion of the preservation, the heart rate, left ventricular developed pressure, and coronary flow were measured at 15 minutes, 30 minutes, and 45 minutes after the initiation of reperfusion to assess the cardiac function. Biopsies were also done and mitochondrial scores were counted in two cases of each group for ultrastructural assessment. Result: The present study showed that the change of heart rate was not different between group 1 and group 2, and group 1 and group 3. The heart rate was significantly decreased at 15 minutes in group 4 compared to that of group 1 (p<0.05 by ANCOVA). The heart rate was recovered at 30 minutes and 45 minutes in group 4 with no significant difference compared to that of group 1. The decrease of LVDP was significant at 15 minutes, 30 minutes and 45 minutes in group 4 compared to that of group 1 (p < 0.001 by ANCOVA). Coronary flow was significantly decreased at 15 minutes, 30 minutes, and 45 minutes in group 4 compared to that of group 1 (p < 0.001 by ANCOVA). In ultrastructural assessment, the mean myocardial mitochondrial scores in group 1, group 2, group 3, and group 4 were 1.02$\pm$0.29, 1.52$\pm$0.26, 1.56$\pm$0.45, 2.22$\pm$0.44 respectively. Conclusion: The HTK solution provided excellent myocardial protection regardless of myocardial temperature for 2 hours. But, when ischemic time exceeded 2 hours, the myocardial hemodynamic function and ultrastructural changes were significantly deteriorated at moderate hypotherma (22∼ 24$^{\circ}C$). This indicates that it is recommended to decrease myocardial temperature when myocardial ischemic time exceeds 2 hours with single infusion of HTK solution as a cardioplegia.

• Restorative Dentistry and Endodontics
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• v.26 no.3
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• pp.206-214
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• 2001

근관계의 완전한 충전을 위해 기계적인 근관 확대 및 화학적인 세척은 필수 불가결하다. 근관내 기구 조작 시 근관벽에는 무기물과 유기물로 구성된 도말층이 형성되는데, 이 층은 서서히 분해되어 충전재 주위에서 미세누출을 야기하고, 세균과 그 부산물이 이동할 수 있는 통로를 제공하기 때문에 제거되어야 한다. 현재 이러한 목적을 위해 다양한 기구들과 세척 용액들이 이용되고 있다. 최근에는 근관확대를 위해 엔진 구동형 니켈-티타늄 파일이 개발되어 임상에서 널리 사용되고 있으며, 이러한 엔진 구동형 파일을 사용할 때는 윤활제의 사용이 필수적이다. 현재 시판되고 있는 윤활제들에는 도말층 제거를 위한 EDTA가 함유되어 있다. 따라서, 본 연구의 목적은 엔진 구동형 니켈-티타늄 파일을 이용해 근관을 확대할 경우 이런 윤활제들의 도말층 제거 효과를 비교 평가하는 것이다. 본 실험에는 75개의 치아가 각각 15개씩 5개의 군으로 분류되어 사용되었다. 대조군은 윤활제를 사용하지 않은 경우이고, 실험 1군에서는 윤활제로 RC-PREP$^{TM}$이, 실험 2군에서는 Glyde$^{TM}$가 각각 사용되었고. 실험 3군은 RC-PREP$^{TM}$을 사용한 후 17% EDTA로 처리하였으며. 실험 4군은 Glyde$^{TM}$를 사용한 후 17% EDTA로 처리하였다. 처리된 시편을 절단한 후 주사전자현미경을 통해 시편의 근관의 중앙부와 치근단 부위를 관찰하여 얻어진 결과는 다음과 같다. 1. 대조군과 실험군의 비교시, 대조군에서 더 많은 양의 도말층이 관찰되었고, 이는 통계학적으로 유의성이 있었다 (p<0.01). 2. 윤활제의 종류에 따른 비교 시, Glyde$^{TM}$를 사용한 2군에서 RC-PREPTM을 사용한 1군 보다 더 적은 양의 도말층이 관찰되었지만, 이는 통계학적으로 유의성이 없었다. 3. 윤활제 사용과 EDTA 처리에 따른 효과 비교 시, EDTA로 처리한 3, 4군에서 윤활제만을 사용한 1, 2군 보다 더 적은 양의 도말층이 관찰되었으며, 이는 통계학적으로 유의성 이 있었다(P<0.01). 4. 치근 중앙부와 치근단 부위에서의 도말층 제거 효과 비교 시, 1, 2군에서는 치근 중앙부에서 더 적은 양의 도말층이 관찰되었고, 통계학적으로 유의성이 있었다(P<0.01). 3, 4군에서는 중앙부의 도말층이 더 적었지만 통계학적으로 유의성이 없었다. 이상으로부터 EDTA가 함유된 윤활제를 함께 사용하면서 엔진구동형 니켈-티타늄 파일로 근관확대 시, 근관벽에 형성된 도말층 제거에 대한 파일의 효능이 상승되었다. 그러나, 윤활제의 도말층 제거 효과는 17% EDTA용액으로 근관벽을 처리하는 것보다는 낮았다 따라서, 엔진구동형 니켈-티타늄 파일로 근관확대 시 EDTA가 함유된 윤활제를 함께 사용하는 것이 요구되고, 보다 완벽하게 도말층을 제거하기 위해서는 충전 전에 17% EDTA 용액으로 근관벽을 처리하는 것이 필요하다고 사료된다.

• Journal of Periodontal and Implant Science
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• v.37 no.sup2
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• pp.385-396
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• 2007

This study proposed a new classification system for maxillary anterior alveolar ridge deformities based on CAT-scan implant simulation as a useful concept in order to more precisely predict treatment outcomes and the necessity for ridge augmentation prior to implant placement. The results indicate that a high number of cases in the maxillary anterior area would require augmentation procedures in order to achieve ideal implant placement and restoration.

• The Korean Journal of Nuclear Medicine
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• v.35 no.3
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• pp.168-184
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• 2001

Purpose: KR-30035 (KR), a new MDR reversing agent, has been found to produce a similar degree of increased Tc-99m MIBI uptake in cultured tumor cells over-expressing mdr1 mRNA compared to verapamil (VP), with less cardiovascular effects. We assessed the MDR-reversing ability of KR in vivo, and effects of various doses of KR on MIBI uptake un nude mice hearing P-glycoprotein (P-gp) positive (+) and P-gp negative (-) human tumor xenografts. Methods: P-gp (+) HCT15/CL02 colorectal and P-gp (-) A549 non-small cell cancer cells were inoculated in each flank of 120 nude mice (20 mice ${\times}$ 6 groups). Group 1 (Gr1) mice received 10mg/kg KR i.p. 3 times $({\times}3)$; Gr2, 10mg/kg VP i.p. ${\times}3$; Gr3, 10mg/kg KR i.p. ${\times}2$ + 25mg/kg KR i.p. ${\times}1$; Gr4, 10mg/kg KR i.p. ${\times}2$ + 50mg/kg i.p. ${\times}1$; Gr5, 10mg/kg KR i.p. ${\times}2$ + 25mg/kg KR i.v. ${\times}1$, GrC, controls. The mice were then injected with Tc-99m MIBI and sacrificed after 10 min, 30 min, 90 min and 240 min. Tumor uptake of MIBI (TU) in each group was compared. Results: TU in P-gp (+) and (-) tumors were both higher in Gr1 than Gr2. Washout rate between the 10 min and 4 hours was lower in Gr5 of P-gp (+) cell(0.93) than the control. Percentage increases in TU were higher in P-gp (+) than P-gp (-) tumors with all KR doses. Pgp (+) TU were highest at 10 mon (173% of GrC) and persisted up to 240 min (144%) in Gr3. Larger doses of KR resulted in a lesser degree of increase in P-gp (+) TU at 10 min (130% in Gr4 and 117% un Gr5) and 30 min (178%, 129%), but TU increased by time up to 240 min (177%, 196%). Heart and lung uptakes were markedly increased in Gr4 and Gr5 at 10 and 30 min, likely due to cardiovascular effects. No mice died. Conclusion: These data further suggest that KR that has significantly lower cardiovascular toxicity than verapamil can be used as an active inhibitor of MDR. Even a relatively low dose of KR significantly increased Tc-99m MIBI uptake in P-gp (+) tumors in vivo.

• Journal of Chest Surgery
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• v.34 no.1
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• pp.41-44
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• 2001

배경: 최근 들어 최소 절개술의 도입의 장점으로 입원간의 단축이 거론되고 있다. 이에 본 연구에서는 최소 흉골 절개술(mini-sternotomy)하에 시행된 개심술 환자에 대하여 전향적으로 수술 후 문제가 없는 환아에 대하여 5일 이내에 퇴원을 시도하고 그 안전성, 경제성 등을 평가하였다. 대상 및 방법: 1998년 11월부터 1999년 7월까지 최소 흉골 절개술하에 개심술을 시행한 환자 중 5일 이내에 퇴원한 환자 29명과 1997년 1월부터 5월까지 전통적인 흉골 절개술로 개심술을 시행한 29명의 환아들을 각각 실험군(제1군)과 대조군(제2군)으로 하였다. 환자는 동일한 수술자로부터 수술을 받았고 질병군은 유사하였다. 결과: 제1군과 제2군의 수술 후 재원일수, 총 병원비, 합병증, 수술 후 소견 등에 차이가 있는지 비교하였다. 평균 수술 후 재원일수는 4.5$\pm$0.6일 및 9.1$\pm$2.4일이었다(p<0.05). 두 환자군에서 수술 후 합병증으로 인한 재입원은 없었고 수술 후 시행한 심초음파에서도 심장수술과 관련된 병변은 관찰되지 않았다. 제1군의 총 입원비의 평균은 7,333,184$\pm$113원이었고, 환자부담액은 3,464,383$\pm$80원이었다. 반면 제2군에서의 총 입원비의 평균은 7,486,136$\pm$140원, 환자부담액은 3,660,194$\pm$92원이었다(p>0.05). 결론: 본 연구를 통해 단기 퇴원 지침하에 환자를 치료함으로써 수술 후 재원일수를 단축시키는 시도가 합병증이나 잔존 병변을 남기지 않고 안정적으로 시행될 수 있다는 사실을 확인하였다. 하지만 경제적 이점은 통계적인 유의성을 찾을 수 없었다. 향후 환자군의 크기와 질병군의 난이도 등을 조절한 비교 평가가 필요하다고 생각된다.

• The Journal of the Korean dental association
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• v.18 no.1 s.130
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• pp.55-58
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• 1980

치주질환 발생시의 대식세포의 형태적 변화 및 기능적인 상관관계를 연구하고저 성견 5마리를 사용 실험하였다. 실험동물을 전신마취한 후 상하악 우측 제 1 및 제 2 소구치의 치경부에 1.3mm굵기의 wire를 4주간 결찰하여 인위적으로 치주염을 유발시켜 실험적으로 하였고, 동일동물의 좌측 제 1 및 제 2 소구치를 대조군으로 하였다. 실험군 및 대조군의 치아협측치은을 0.5 0.5 1mm의 형태로 절제하여 전자현미경적 관찰을 위한 통법의 조직처리를 한 다음 관찰하였다. 그 관찰 결과를 보면 다음과 같았다. 1. 실첨군의 치은의 고유층에 탐식성 돌기를 가진 많은 대식세포들이 출현하였다. 2. 변성된 교원 섬유군 인접부위에 phagocytic vesicle을 가진 대식세포들의 세포질내에 지방과립 및 잔존물을 볼 수 있었다. 한편 RER 및 Golgi complex는 매우 적었다. 3. 세포막은 그 기능상으로 많은 ruffles or folds를 나타냈으며 이 물질을 포식하는 양식을 뛰기도 하였다. pinocytotic activity를 띄는 대식세포들도 보였다. 4. 신생섬유아세포 주위에 밀접한 관계를 띄고 대식세포가 나타났으며 상호 유사하다. 대식세포에는 많은 residual body가 나타났고 RER은 매우 적었다.

• Restorative Dentistry and Endodontics
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• v.31 no.4
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• pp.282-289
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• 2006

The purpose of this study was to compare the ability of three resin surface sealants to prevent microleakage in Class V composite resin restorations. Forty Class V cavities with the occlusal margin in enamel and gingival margin in dentin were prepared on the buccal surfaces of sound extracted molars, and restored with composite resin. Restorations were randomly assigned into one of four equal groups (n = 10): a control group, without resin sealing, and three experimental groups in which margins were sealed with Fortify Plus, Biscover and Permaseal, respectively. Specimens were thermocycled, immersed in a 2% methylene blue solution for 4 hours, sectioned longitudinally, and observed the leakage at the occlusal and gingival margins. The result was analyzed using Kruskal-Wallis test, Mann-Whitney test and Wilcoxon signed rank test. In conclusion, the ability to reduce microleakage at occlusal margins was similar in all of three sealants. However at gingival margin, it depended on the type of used resin surface sealant. At gingival margin. control and Fortify Plus group showed statistically higher microleakage than PermaSeal group. and Fortify Plus group also showed higher microleakage than BisCover group (p < 0.05).

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.209-218
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• 2018

This study was carried out to investigate the occurrence and distribution of ALS inhibiting herbicide-resistant weeds and to estimate the appeared areas of resistant weeds in the paddy fields of Gyeongnam province of Korea in 2017 and 2018 using a soil assay method. Compared with the 2012 survey, this study showed that the infested ratio of ALS inhibiting herbicide-resistant weeds increased from 1.0% to 66.8% and the infested area increased from 876 ha to 49,008 ha. The infested area of ALS inhibiting herbicide-resistant weeds was estimated in Ulsan-si (8.4%), Hapcheon-gun (8.3%), Haman-gun (7.9%), Goseong-gun (7.9%), Hadong-gun (7.3%), Jinju-si (7.2%), Changnyeong-gun (7.0%), Gimhae-si (6.4%), Miryang-si (5.5%), Busan-si (4.9%), Uiryeong-gun (4.6%), Namhae-gun (4.3%), Geochang-gun (4.2%), Changwon-si (3.8%), Geoje-si (2.9%), Yangsan-si (1.8%), Sancheong-gun (0.9%) and Tongyeong-si (0.4%), and the herbicide resistant weeds was not occurred in Hamyang-gun. The most dominant ALS inhibiting herbicide-resistant weeds in paddy fields were Monochoria vaginalis, followed by Echinochloa oryzicola, Lindernia dubia, Scirpus juncoides, Ludwigia prostrata, Cyperus difformis, Sagittaria trifolia and Rotala indica. ALS inhibiting herbicide-resistant M. vaginalis, L. dubia, and E. oryzoides occurred throughout Gyeongnam province, and ALS inhibiting herbicide-resistant S. trifolia and R. indica were only found in Gimhae-si. Therefore, these results will be utilized to estimate population dynamics of ALS inhibiting herbicide-resistant weeds and provide proper management practices in the paddy fields of Gyeongnam province.

• Tuberculosis and Respiratory Diseases
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• v.40 no.2
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• pp.123-134
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• 1993

Background: Tissue-type plasminogen activator is a physiologic activator, which has high affinity for fibrin and is activated by fibrin. Because of these properties, t-PA has the potential to induce effective thrombolysis without producing a systemic lytic state. In practice, however, therapeutically efficacious doses of t-PA has been associated with the development of a systemic lytic state. As experience with t-PA has accumulated, it has suggested that the fibrin selectivity is influenced by the dose and duration of t-PA infusion, and many studies have performed in an attempt to optimize the duration of t-PA regimen. Methods: This study was designed to assess the thrombolytic efficacy of t-PA and the differences of two dosing regimens of t-PA (infusion of 1 mg/kg t-PA over 15 or 180 minutes) in a canine model of pulmonary embolism, induced by injection of radioactive autologous blood clots. By continuously counting over both lung fields with a external gamma counter, we correlated rate and extent of pulmonary thrombolysis with corresponding pulmonary hemodynamics in addition to the gas analyses of arterial and mixed venous blood. Results: 1) While total clot lysis was similar ($36.2{\pm}3.3%$ and $39.6{\pm}2.3%$ respectively, p>0.05) when t-PA was infused over 15 or 180 minutes, the rate of lysis during infusion was markedly increased with the shorter infusion ($81.4{\pm}16.8%/hr$ vs $37.3{\pm}2.4%/hr$, p<0.05). 2) The duration of thrombolysis was $63.3{\pm}22.2$ minutes although t-PA was administered over 15 minutes, and it was only $148.5{\pm}14.0$ minutes in case of the infusion over 180 minutes (p<0.05). 3) The increased rate of thrombolysis with the shorter infusion was accompanied by a faster amelioration of cardiopulmonary impairment from pulmonary embolism (p<0.05). Conclusion: It is concluded that the shorter (15 minutes) infusion of t-PA is superior to the longer (180 minutes) infusion when the dose is equal, in consideration of the faster improvement in cardiopulmonary impairment from pulmonary embolism.